MED6

Summary

Gene Symbol: MED6
Description: mediator complex subunit MED6
Alias: mediator complex subunit MED6
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Takahashi H, Kasahara K, Kokubo T. Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation. Genes Cells. 2009;14:53-67 pubmed publisher
    ..Add-back experiments indicate that some unidentified factor(s) in med9 extracts may impact the binding of TFIID to the promoter. ..
  2. Baidoobonso S, Guidi B, Myers L. Med19(Rox3) regulates Intermodule interactions in the Saccharomyces cerevisiae mediator complex. J Biol Chem. 2007;282:5551-9 pubmed
    ..Although the Middle module is unnecessary for holding the Head and Tail modules together, it is required for the complex to function as a conduit between activators and the core transcription machinery. ..
  3. Imasaki T, Calero G, Cai G, Tsai K, Yamada K, Cardelli F, et al. Architecture of the Mediator head module. Nature. 2011;475:240-3 pubmed publisher
    ..Our results reveal architectural principles underlying the role of Mediator in the regulation of gene expression. ..
  4. Lee Y, Park J, Min S, Han S, Kim Y. An activator binding module of yeast RNA polymerase II holoenzyme. Mol Cell Biol. 1999;19:2967-76 pubmed
  5. Lariviere L, Seizl M, van Wageningen S, Röther S, van de Pasch L, Feldmann H, et al. Structure-system correlation identifies a gene regulatory Mediator submodule. Genes Dev. 2008;22:872-7 pubmed publisher
    ..The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation. ..
  6. Gromöller A, Lehming N. Srb7p is a physical and physiological target of Tup1p. EMBO J. 2000;19:6845-52 pubmed
    ..Our finding that Med6p and Tup1p compete for the interaction with Srb7p suggests a model for Tup1p-mediated repression. ..
  7. Cai G, Imasaki T, Takagi Y, Asturias F. Mediator structural conservation and implications for the regulation mechanism. Structure. 2009;17:559-67 pubmed publisher
    ..This suggests that the topology and structural dynamics of Mediator constitute important elements of a conserved regulation mechanism. ..
  8. Baumli S, Hoeppner S, Cramer P. A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer. J Biol Chem. 2005;280:18171-8 pubmed
    ..sites on the surface allow for assembly of the Mediator middle module and for binding of the conserved subunit MED6, which is shown to bridge to the Mediator head module. A flexible MED6 bridge and the MED7...
  9. Lorch Y, Beve J, Gustafsson C, Myers L, Kornberg R. Mediator-nucleosome interaction. Mol Cell. 2000;6:197-201 pubmed
    ..Sequence alignment shows significant similarity of Nut1 to the GCN5-related N-acetyltransferase superfamily. Finally, recombinant Nut1 exhibits HAT activity in an in-gel assay. ..

More Information

Publications31

  1. Myers L, Gustafsson C, Bushnell D, Lui M, Erdjument Bromage H, Tempst P, et al. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev. 1998;12:45-54 pubmed
    ..Evidence for human homologs of several mediator proteins, including Med7, points to similar mechanisms in higher cells. ..
  2. Han S, Lee J, Kang J, Kim Y. Med9/Cse2 and Gal11 modules are required for transcriptional repression of distinct group of genes. J Biol Chem. 2001;276:37020-6 pubmed
  3. Takagi Y, Calero G, Komori H, Brown J, Ehrensberger A, Hudmon A, et al. Head module control of mediator interactions. Mol Cell. 2006;23:355-64 pubmed
    ..The head module evidently controls Mediator-RNA polymerase II and Mediator-promoter interactions. ..
  4. Balciunas D, Gälman C, Ronne H, Bjorklund S. The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression. Proc Natl Acad Sci U S A. 1999;96:376-81 pubmed
    ..Finally, we show that the Med2 protein is lost from the mediator on purification from Med1-deficient cells, indicating a physical interaction between Med1 and Med2. ..
  5. Hallberg M, Hu G, Tronnersjö S, Shaikhibrahim Z, Balciunas D, Bjorklund S, et al. Functional and physical interactions within the middle domain of the yeast mediator. Mol Genet Genomics. 2006;276:197-210 pubmed
    ..In the yeast Saccharomyces cerevisiae, Med21 is known to interact both with the Mediator subunit Med6 and the global co-repressor Tup1...
  6. Keogh M, Podolny V, Buratowski S. Bur1 kinase is required for efficient transcription elongation by RNA polymerase II. Mol Cell Biol. 2003;23:7005-18 pubmed
    ..These results show that Bur1 functions in transcription elongation but may phosphorylate a substrate other than the CTD. ..
  7. Lee T, Wyrick J, Koh S, Jennings E, Gadbois E, Young R. Interplay of positive and negative regulators in transcription initiation by RNA polymerase II holoenzyme. Mol Cell Biol. 1998;18:4455-62 pubmed
    ..We report here that MED6 and SRB6, both of which encode essential components of the holoenzyme, are among the dominant suppressors and that ..
  8. Zhong P, Melcher K. Identification and characterization of the activation domain of Ifh1, an activator of model TATA-less genes. Biochem Biophys Res Commun. 2010;392:77-82 pubmed publisher
  9. Yudkovsky N, Ranish J, Hahn S. A transcription reinitiation intermediate that is stabilized by activator. Nature. 2000;408:225-9 pubmed
    ..The scaffold is stabilized in the presence of the activator Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription. ..
  10. Eyboulet F, Wydau Dematteis S, Eychenne T, Alibert O, Neil H, Boschiero C, et al. Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo. Nucleic Acids Res. 2015;43:9214-31 pubmed publisher
    ..This study provides an extensive genome-wide view of Mediator's role in PIC formation, suggesting that Mediator coordinates multiple steps of a PIC assembly pathway. ..
  11. Petty E, Lafon A, Tomlinson S, Mendelsohn B, Pillus L. Promotion of Cell Viability and Histone Gene Expression by the Acetyltransferase Gcn5 and the Protein Phosphatase PP2A in Saccharomyces cerevisiae. Genetics. 2016;203:1693-707 pubmed publisher
    ..These data highlight the potential significance of dynamic phosphorylation and dephosphorylation of these deeply conserved histone residues for cell viability. ..
  12. Lee Y, Min S, Gim B, Kim Y. A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans. Mol Cell Biol. 1997;17:4622-32 pubmed
    ..A database search revealed the existence of MED6-related genes in humans and Caenorhabditis elegans, suggesting that the role of mediator in transcriptional ..
  13. Cai G, Imasaki T, Yamada K, Cardelli F, Takagi Y, Asturias F. Mediator head module structure and functional interactions. Nat Struct Mol Biol. 2010;17:273-9 pubmed publisher
  14. Gustafsson C, Myers L, Li Y, Redd M, Lui M, Erdjument Bromage H, et al. Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme. J Biol Chem. 1997;272:48-50 pubmed
  15. Eychenne T, Novikova E, Barrault M, Alibert O, Boschiero C, Peixeiro N, et al. Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture. Genes Dev. 2016;30:2119-2132 pubmed
    ..This study thus provides mechanistic insights into the coordinated function of Mediator and TFIIB in PIC assembly in different chromatin contexts. ..
  16. Esnault C, Ghavi Helm Y, Brun S, Soutourina J, Van Berkum N, Boschiero C, et al. Mediator-dependent recruitment of TFIIH modules in preinitiation complex. Mol Cell. 2008;31:337-46 pubmed publisher
    ..We conclude that the Mediator head module plays a critical role in TFIIH and TFIIE recruitment to the PIC. We identify steps in PIC formation that suggest a branched assembly pathway. ..
  17. Ang K, Ee G, Ang E, Koh E, Siew W, Chan Y, et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol. 2012;10:e1001290 pubmed publisher
    ..The ability of Mediator to control the protein degradation of transcriptional inhibitors indicates that Mediator is actually able to direct its own recruitment to gene promoters. ..
  18. Miller C, Matic I, Maier K, Schwalb B, Roether S, Strasser K, et al. Mediator phosphorylation prevents stress response transcription during non-stress conditions. J Biol Chem. 2012;287:44017-26 pubmed publisher
    ..Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells. ..
  19. Lee Y, Kim Y. Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription. Mol Cell Biol. 1998;18:5364-70 pubmed
    ..In particular, MED6 is essential for activated transcription from many class II promoters, suggesting that it functions as a key player ..
  20. Koschubs T, Lorenzen K, Baumli S, Sandström S, Heck A, Cramer P. Preparation and topology of the Mediator middle module. Nucleic Acids Res. 2010;38:3186-95 pubmed publisher
    ..The subunits, Med1 and Med10, which bridge to the Mediator tail module, bind to both Med7 and Med4. ..
  21. Liu Z, Myers L. Med5(Nut1) and Med17(Srb4) are direct targets of mediator histone H4 tail interactions. PLoS ONE. 2012;7:e38416 pubmed publisher
    ..This analysis has identified the Med5 subunit of Mediator as a target for histone tail interactions and suggests that the previously observed effect of med5 mutations on telomeric heterochromatin and silencing is direct. ..
  22. Han S, Lee Y, Gim B, Ryu G, Park S, Lane W, et al. Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation. Mol Cell Biol. 1999;19:979-88 pubmed
    ..Med11 was also required specifically for MFalpha1 transcription. On the other hand, Med6 was required for all of these transcriptional activation processes...