Gene Symbol: MDH3
Description: malate dehydrogenase MDH3
Alias: malate dehydrogenase MDH3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Tsvetanova N, Klass D, Salzman J, Brown P. Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae. PLoS ONE. 2010;5: pubmed publisher
    ..Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation. ..
  2. Elgersma Y, Vos A, Van den Berg M, van Roermund C, van der Sluijs P, Distel B, et al. Analysis of the carboxyl-terminal peroxisomal targeting signal 1 in a homologous context in Saccharomyces cerevisiae. J Biol Chem. 1996;271:26375-82 pubmed a homologous context, using Saccharomyces cerevisiae and endogenous peroxisomal malate dehydrogenase (MDH3)...
  3. McAlister Henn L, Small W. Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol. 1997;57:317-39 pubmed
  4. Scherrer T, Mittal N, Janga S, Gerber A. A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes. PLoS ONE. 2010;5:e15499 pubmed publisher
    ..Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation. ..
  5. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
  6. Steffan J, McAlister Henn L. Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase. J Biol Chem. 1992;267:24708-15 pubmed
    The MDH3 isozyme of Saccharomyces cerevisiae was purified from a haploid strain containing disruptions in genomic loci encoding the mitochondrial MDH1 and nonmitochondrial MDH2 isozymes...
  7. Minard K, McAlister Henn L. Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase. Mol Cell Biol. 1991;11:370-80 pubmed
    ..The detection of residual malate dehydrogenase activity in the latter strain confirmed the existence of at least three isozymes in yeast cells. ..
  8. van Roermund C, Elgersma Y, Singh N, Wanders R, Tabak H. The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions. EMBO J. 1995;14:3480-6 pubmed
    ..Disruption of the peroxisomal malate dehydrogenase 3 gene (MDH3) resulted in impaired beta-oxidation capacity as measured in intact cells, whereas beta-oxidation was perfectly ..
  9. McAlister Henn L, Steffan J, Minard K, Anderson S. Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast. J Biol Chem. 1995;270:21220-5 pubmed
    The malate dehydrogenase isozyme MDH3 of Saccharomyces cerevisiae was found to be localized to peroxisomes by cellular fractionation and density gradient centrifugation...

More Information


  1. Bottger G, Barnett P, Klein A, Kragt A, Tabak H, Distel B. Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell. 2000;11:3963-76 pubmed
    ..Taken together, this indicates that in the absence of Pex13-SH3 interaction, other protein(s) is able to bind Pex5p at the peroxisome; Pex14p is a likely candidate for this function. ..
  2. Marelli M, Smith J, Jung S, Yi E, Nesvizhskii A, Christmas R, et al. Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane. J Cell Biol. 2004;167:1099-112 pubmed
    ..Rho1p regulates the assembly state of actin on the peroxisome membrane, thereby controlling peroxisome membrane dynamics and biogenesis. ..
  3. Kerssen D, Hambruch E, Klaas W, Platta H, de Kruijff B, Erdmann R, et al. Membrane association of the cycling peroxisome import receptor Pex5p. J Biol Chem. 2006;281:27003-15 pubmed
    ..Altogether, these data strongly suggest that a translocation-competent state of the PTS1 receptor enters the membrane via protein-lipid interactions before it tightly associates with other peroxins. ..
  4. Klein A, Barnett P, Bottger G, Konings D, Tabak H, Distel B. Recognition of peroxisomal targeting signal type 1 by the import receptor Pex5p. J Biol Chem. 2001;276:15034-41 pubmed
    ..Based on the mutational analysis and the structural model, we put forward a model as to how PTS1 proteins are selected by Pex5p. ..
  5. Klein A, van den Berg M, Bottger G, Tabak H, Distel B. Saccharomyces cerevisiae acyl-CoA oxidase follows a novel, non-PTS1, import pathway into peroxisomes that is dependent on Pex5p. J Biol Chem. 2002;277:25011-9 pubmed
  6. Kurita O. Overexpression of peroxisomal malate dehydrogenase MDH3 gene enhances cell death on H2O2 stress in the ald5 mutant of Saccharomyces cerevisiae. Curr Microbiol. 2003;47:192-7 pubmed
    ..However, overexpression of the MDH3 gene that was involved in the reoxidation of NADH during fatty acid beta-oxidation caused a decrease in cell ..
  7. Zelle R, de Hulster E, van Winden W, de Waard P, Dijkema C, Winkler A, et al. Malic acid production by Saccharomyces cerevisiae: engineering of pyruvate carboxylation, oxaloacetate reduction, and malate export. Appl Environ Microbiol. 2008;74:2766-77 pubmed publisher
    ..overexpression of the native pyruvate carboxylase encoded by PYC2, (ii) high-level expression of an allele of the MDH3 gene, of which the encoded malate dehydrogenase was retargeted to the cytosol by deletion of the C-terminal ..