MCM10

Summary

Gene Symbol: MCM10
Description: Mcm10p
Alias: DNA43, Mcm10p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Merchant A, Kawasaki Y, Chen Y, Lei M, Tye B. A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:3261-71 pubmed
    We describe a new minichromosome maintenance factor, Mcm10, and show that this essential protein is involved in the initiation of DNA replication in Saccharomyces cerevisiae...
  2. Becker J, Nguyen H, Wang X, Bielinsky A. Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair. Cell Cycle. 2014;13:1737-48 pubmed publisher
    b>Mcm10 is a multifunctional replication factor with reported roles in origin activation, polymerase loading, and replication fork progression...
  3. Lee C, Liachko I, Bouten R, Kelman Z, Tye B. Alternative mechanisms for coordinating polymerase alpha and MCM helicase. Mol Cell Biol. 2010;30:423-35 pubmed publisher
    ..the MCM helicase enhance the physical stability of DNA polymerase alpha in the absence of their presumed linker, Mcm10. Mcm10 is an essential DNA replication protein implicated in the stable assembly of the replisome by virtue of its ..
  4. Ricke R, Bielinsky A. Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Mol Cell. 2004;16:173-85 pubmed
    b>Mcm10 is a conserved eukaryotic DNA replication factor whose function has remained elusive. We report here that Mcm10 binding to replication origins in budding yeast is cell cycle regulated and dependent on the putative helicase, Mcm2-7...
  5. Das Bradoo S, Ricke R, Bielinsky A. Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast. Mol Cell Biol. 2006;26:4806-17 pubmed
    The minichromosome maintenance protein 10 (Mcm10) is an evolutionarily conserved factor that is essential for replication initiation and elongation...
  6. Ricke R, Bielinsky A. A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast. J Biol Chem. 2006;281:18414-25 pubmed
    b>Mcm10 is a conserved eukaryotic DNA replication factor that is required for S phase progression. Recently, Mcm10 has been shown to interact physically with the DNA polymerase-alpha (pol-alpha).primase complex...
  7. Quan Y, Xia Y, Liu L, Cui J, Li Z, Cao Q, et al. Cell-Cycle-Regulated Interaction between Mcm10 and Double Hexameric Mcm2-7 Is Required for Helicase Splitting and Activation during S Phase. Cell Rep. 2015;13:2576-2586 pubmed publisher
    ..Interestingly, Mcm10, a robust MCM interactor, co-purifies exclusively with the DHs in the context of chromatin...
  8. Sawyer S, Cheng I, Chai W, Tye B. Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae. J Mol Biol. 2004;340:195-202 pubmed
    b>Mcm10 has recently been found to play a crucial role in multiple steps of the DNA replication initiation process in eukaryotes...
  9. Langston L, Mayle R, Schauer G, Yurieva O, Zhang D, Yao N, et al. Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks. elife. 2017;6: pubmed publisher
    ..This report demonstrates that Mcm10, an essential replication protein unique to eukaryotes, binds CMG and greatly stimulates its helicase activity in ..

More Information

Publications31

  1. Eisenberg S, Korza G, Carson J, Liachko I, Tye B. Novel DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae. J Biol Chem. 2009;284:25412-20 pubmed publisher
    The Mcm10 protein is essential for chromosomal DNA replication in eukaryotic cells. We purified the Saccharomyces cerevisiae Mcm10 (ScMcm10) and characterized its DNA binding properties...
  2. Tittel Elmer M, Alabert C, Pasero P, Cobb J. The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress. EMBO J. 2009;28:1142-56 pubmed publisher
  3. Douglas N, Dozier S, Donato J. Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci. Mol Biol Rep. 2005;32:197-204 pubmed
    ..Our ongoing studies of several mutants of the replication initiation factor Mcm10 of budding yeast revealed an associated defect in the production of mating type pheromones...
  4. Liachko I, Tye B. Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Genetics. 2005;171:503-15 pubmed
    ..Consistent with a direct silencing function, Mcm10p shows a two-hybrid interaction with Sir2p and Sir3p that is destroyed by the mcm10-1 mutation and dependent on the ..
  5. Araki Y, Kawasaki Y, Sasanuma H, Tye B, Sugino A. Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Genes Cells. 2003;8:465-80 pubmed
    MCM10 is essential for the initiation of chromosomal DNA replication in Saccharomyces cerevisiae. Mcm10p functionally interacts with components of the pre-replicative complex (Mcm2-Mcm7 complex and origin recognition complex) as well as ..
  6. Homesley L, Lei M, Kawasaki Y, Sawyer S, Christensen T, Tye B. Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev. 2000;14:913-26 pubmed
    ..action of MCM2-7 during the transition from initiation to elongation replication, we have focused our studies on Mcm10, a replication initiation protein that physically interacts with members of the MCM2-7 complex...
  7. Lõoke M, Maloney M, Bell S. Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase. Genes Dev. 2017;31:291-305 pubmed publisher
    ..binding of the helicase-activating proteins Cdc45 and GINS (forming the CMG complex), an additional protein, Mcm10, drives initial origin DNA unwinding by an unknown mechanism...
  8. Kawasaki Y, Hiraga S, Sugino A. Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae. Genes Cells. 2000;5:975-89 pubmed
    ..Previous work showed that Mcm10p interacts with the Mcm2-7 protein complex that may be functioning as the replication-licensing factor...
  9. Alver R, Zhang T, Josephrajan A, Fultz B, Hendrix C, Das Bradoo S, et al. The N-terminus of Mcm10 is important for interaction with the 9-1-1 clamp and in resistance to DNA damage. Nucleic Acids Res. 2014;42:8389-404 pubmed publisher
    Accurate replication of the genome requires the evolutionarily conserved minichromosome maintenance protein, Mcm10. Although the details of the precise role of Mcm10 in DNA replication are still debated, it interacts with the Mcm2-7 core ..
  10. van Deursen F, Sengupta S, De Piccoli G, Sanchez Diaz A, Labib K. Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation. EMBO J. 2012;31:2195-206 pubmed publisher
    b>Mcm10 is essential for chromosome replication in eukaryotic cells and was previously thought to link the Mcm2-7 DNA helicase at replication forks to DNA polymerase alpha...
  11. Lydeard J, Lipkin Moore Z, Sheu Y, Stillman B, Burgers P, Haber J. Break-induced replication requires all essential DNA replication factors except those specific for pre-RC assembly. Genes Dev. 2010;24:1133-44 pubmed publisher
    ..These results suggest that origin-independent BIR involves cross-talk between normal DNA replication factors and PRR. ..
  12. Muramatsu S, Hirai K, Tak Y, Kamimura Y, Araki H. CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast. Genes Dev. 2010;24:602-12 pubmed publisher
    ..We propose that CDK regulates the initiation of DNA replication in budding yeast through formation of the pre-LC. ..
  13. Burich R, Lei M. Two bipartite NLSs mediate constitutive nuclear localization of Mcm10. Curr Genet. 2003;44:195-201 pubmed
    ..of bipartite NLSs in constitutively nuclear pre-RC components, including the origin recognition complex, Mcm10 and Noc3. Interestingly, in a subgroup of these pre-RC components, two putative NLSs were found in each protein...
  14. Zhang T, Fultz B, Das Bradoo S, Bielinsky A. Mapping ubiquitination sites of S. cerevisiae Mcm10. Biochem Biophys Rep. 2016;8:212-218 pubmed publisher
    ..As a multivalent DNA scaffold, Mcm10 coordinates the action of proteins that are indispensable for lagging strand synthesis, such as the replication ..
  15. Watase G, Takisawa H, Kanemaki M. Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS. Curr Biol. 2012;22:343-9 pubmed publisher
    ..b>Mcm10 is an evolutionally conserved essential protein required for the initiation of replication [3, 4]...
  16. Wang J, Wu R, Lu Y, Liang C. Ctf4p facilitates Mcm10p to promote DNA replication in budding yeast. Biochem Biophys Res Commun. 2010;395:336-41 pubmed publisher
    ..Furthermore, Ctf4p interacts with Mcm10p physically and genetically, and the chromatin association of Ctf4p depends on Mcm10p...
  17. Cook C, Kung G, Peterson F, Volkman B, Lei M. A novel zinc finger is required for Mcm10 homocomplex assembly. J Biol Chem. 2003;278:36051-8 pubmed
    b>Mcm10 is a DNA replication factor that interacts with multiple subunits of the MCM2-7 hexameric complex. We report here that Mcm10 self-interacts and assembles into large homocomplexes (approximately 800 kDa)...
  18. Douglas M, Diffley J. Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex. J Biol Chem. 2016;291:5879-88 pubmed publisher
    b>Mcm10 is required for the initiation of eukaryotic DNA replication and contributes in some unknown way to the activation of the Cdc45-MCM-GINS (CMG) helicase...
  19. Thu Y, Van Riper S, Higgins L, Zhang T, Becker J, Markowski T, et al. Slx5/Slx8 Promotes Replication Stress Tolerance by Facilitating Mitotic Progression. Cell Rep. 2016;15:1254-65 pubmed publisher
    Loss of minichromosome maintenance protein 10 (Mcm10) causes replication stress. We uncovered that S. cerevisiae mcm10-1 mutants rely on the E3 SUMO ligase Mms21 and the SUMO-targeted ubiquitin ligase complex Slx5/8 for survival...
  20. Pérez Arnaiz P, Kaplan D. An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation. J Mol Biol. 2016;428:4608-4625 pubmed publisher
    b>Mcm10 is an essential protein that functions to initiate DNA replication after the formation of the replication fork helicase...
  21. Pérez Arnaiz P, Bruck I, Colbert M, Kaplan D. An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-? to Mcm2-7. Nucleic Acids Res. 2017;45:7261-7275 pubmed publisher
    b>Mcm10 is an essential eukaryotic factor required for DNA replication. The replication fork helicase is composed of Cdc45, Mcm2-7 and GINS (CMG)...
  22. Liachko I, Tye B. Mcm10 mediates the interaction between DNA replication and silencing machineries. Genetics. 2009;181:379-91 pubmed publisher
    ..Another replication factor, Mcm10, mediates the interaction between these replication and silencing proteins via a short C-terminal domain...