Gene Symbol: MCM1
Description: transcription factor MCM1
Alias: FUN80, transcription factor MCM1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Boros J, Lim F, Darieva Z, Pic Taylor A, Harman R, Morgan B, et al. Molecular determinants of the cell-cycle regulated Mcm1p-Fkh2p transcription factor complex. Nucleic Acids Res. 2003;31:2279-88 pubmed
    ..Our data therefore provide molecular insights into the mechanisms of Mcm1p- Fkh2p complex formation and more generally aid our understanding of MADS-box protein function. ..
  2. Pramila T, Miles S, GuhaThakurta D, Jemiolo D, Breeden L. Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle. Genes Dev. 2002;16:3034-45 pubmed
    ..These proteins bind to Mcm1 and to a typical homeodomain binding site...
  3. Shore P, Sharrocks A. The MADS-box family of transcription factors. Eur J Biochem. 1995;229:1-13 pubmed
    ..within the DNA-binding domains of these proteins and the name refers to four of the originally identified members: MCM1, AG, DEFA and SRF. Several proteins within this family have significant biological roles...
  4. Mead J, Zhong H, Acton T, Vershon A. The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes. Mol Cell Biol. 1996;16:2135-43 pubmed
    ..We have examined how the yeast (Saccharomyces cerevisiae) homeodomain protein alpha2 specifically interacts with Mcm1, a MADS box protein, to bind DNA specifically and repress transcription...
  5. Koranda M, Schleiffer A, Endler L, Ammerer G. Forkhead-like transcription factors recruit Ndd1 to the chromatin of G2/M-specific promoters. Nature. 2000;406:94-8 pubmed
    ..Its recruitment depends on a permanent protein-DNA complex consisting of the MADS box protein, Mcm1, and a recently identified partner Fkh2, a forkhead/winged helix related transcription factor...
  6. Passmore S, Elble R, Tye B. A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes. Genes Dev. 1989;3:921-35 pubmed
    The Saccharomyces cerevisiae MCM1 gene product is a protein with multiple functions. It is a transcription factor necessary for expression of mating-type-specific genes and is also required for the maintenance of minichromosomes...
  7. Althoefer H, Schleiffer A, Wassmann K, Nordheim A, Ammerer G. Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:5917-28 pubmed
    In the budding yeast Saccharomyces cerevisiae, MCM1 encodes an essential DNA-binding protein that regulates transcription of many genes in cooperation with different associated factors...
  8. Mead J, Bruning A, Gill M, Steiner A, Acton T, Vershon A. Interactions of the Mcm1 MADS box protein with cofactors that regulate mating in yeast. Mol Cell Biol. 2002;22:4607-21 pubmed
    The yeast Mcm1 protein is a member of the MADS box family of transcriptional regulatory factors, a class of DNA-binding proteins that control numerous cellular and developmental processes in yeast, Drosophila melanogaster, plants, and ..
  9. Hollenhorst P, Pietz G, Fox C. Mechanisms controlling differential promoter-occupancy by the yeast forkhead proteins Fkh1p and Fkh2p: implications for regulating the cell cycle and differentiation. Genes Dev. 2001;15:2445-56 pubmed
    ..Multiple Fkh-binding sites may be a distinguishing feature of bona fide Fkh promoters in yeast and other organisms. ..

More Information


  1. Loy C, Lydall D, Surana U. NDD1, a high-dosage suppressor of cdc28-1N, is essential for expression of a subset of late-S-phase-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:3312-27 pubmed
    ..Both CLB2 expression and its upregulation by NDD1 are mediated by a 240-bp promoter sequence that contains four MCM1-binding sites...
  2. Tan S, Richmond T. Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex. Nature. 1998;391:660-6 pubmed
    The structure of a complex containing the homeodomain repressor protein MATalpha2 and the MADS-box transcription factor MCM1 bound to DNA has been determined by X-ray crystallography at 2.25 A resolution...
  3. McInerny C, Partridge J, Mikesell G, Creemer D, Breeden L. A novel Mcm1-dependent element in the SWI4, CLN3, CDC6, and CDC47 promoters activates M/G1-specific transcription. Genes Dev. 1997;11:1277-88 pubmed
    ..The ECB element contains an Mcm1 binding site to which Mcm1 binds in vitro, and an Mcm1-VP16 fusion, which places a constitutive activator on Mcm1-..
  4. Pic A, Lim F, Ross S, Veal E, Johnson A, Sultan M, et al. The forkhead protein Fkh2 is a component of the yeast cell cycle transcription factor SFF. EMBO J. 2000;19:3750-61 pubmed
    In the yeast Saccharomyces cerevisiae, the MADS-box protein Mcm1, which is highly related to mammalian SRF (serum response factor), forms a ternary complex with SFF (Swi five factor) to regulate the cell cycle expression of genes such as ..
  5. Kumar R, Reynolds D, Shevchenko A, Goldstone S, Dalton S. Forkhead transcription factors, Fkh1p and Fkh2p, collaborate with Mcm1p to control transcription required for M-phase. Curr Biol. 2000;10:896-906 pubmed
    ..on both the SWI5 and CLB2 cell-cycle-regulated upstream activating sequence (UAS) elements in vitro, and in an Mcm1 p-dependent manner in vivo...
  6. Darieva Z, Clancy A, Bulmer R, Williams E, Pic Taylor A, Morgan B, et al. A competitive transcription factor binding mechanism determines the timing of late cell cycle-dependent gene expression. Mol Cell. 2010;38:29-40 pubmed publisher
  7. Sawyer S, Cheng I, Chai W, Tye B. Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae. J Mol Biol. 2004;340:195-202 pubmed
    ..Our results show that interaction between the Mcm10 and Cdc45 proteins facilitates the recruitment of Cdc45 onto the ARS1 origin. ..
  8. Guzinska K, Varghese R, Vancura A. Role of Plc1p in regulation of Mcm1p-dependent genes. FEMS Microbiol Lett. 2009;295:245-50 pubmed publisher
    ..However, Plc1p is not involved in stabilization of Mcm1p and affects transcription of Mcm1p-dependent genes by a different mechanism, probably involving regulation of chromatin remodeling complexes. ..
  9. Primig M, Winkler H, Ammerer G. The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins. EMBO J. 1991;10:4209-18 pubmed
    The MCM1 gene encodes an essential DNA binding protein that, in cooperation with the transactivators alpha 1 and STE12 and the repressor alpha 2, confers mating specificity to haploid yeast cells...
  10. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
  11. Bruhn L, Sprague G. MCM1 point mutants deficient in expression of alpha-specific genes: residues important for interaction with alpha 1. Mol Cell Biol. 1994;14:2534-44 pubmed
    Complexes formed between MCM1 and several coregulatory proteins--alpha 1, alpha 2, and STE12--serve to govern transcription of the a- and alpha-specific gene sets in the yeast Saccharomyces cerevisiae...
  12. Yoon S, Hinnebusch A. Mcm1p binding sites in ARG1 positively regulate Gcn4p binding and SWI/SNF recruitment. Biochem Biophys Res Commun. 2009;381:123-8 pubmed publisher
    ..We propose that Mcm1p contributes to active transcription at the ARG1 promoter by increasing the binding of the activator Gcn4p and by recruiting the co-activator complex SWI/SNF at ARG1 under Gcn4p-induced conditions. ..
  13. Kamei Y, Tai A, Dakeyama S, Yamamoto K, Inoue Y, Kishimoto Y, et al. Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast. Biochem Biophys Res Commun. 2015;463:351-6 pubmed publisher
    ..Heterozygous diploid knockout strains for FHL1, RAP1, REB1, and MCM1 genes showed significantly short lifespan...
  14. Bastajian N, Friesen H, Andrews B. Bck2 acts through the MADS box protein Mcm1 to activate cell-cycle-regulated genes in budding yeast. PLoS Genet. 2013;9:e1003507 pubmed publisher
    ..yeast two-hybrid screen using a truncated version of Bck2 and discovered six novel Bck2-binding partners including Mcm1, an essential protein that binds to and activates M/G1 promoters through Early Cell cycle Box (ECB) elements as ..
  15. Messenguy F, Dubois E. Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:2586-92 pubmed
    ..We have shown that MCM1 is part of the ARGR regulatory complex, by in vitro binding experiments, at the ARGR5,6 promoter...
  16. Chen Y, Tye B. The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis. Mol Cell Biol. 1995;15:4631-9 pubmed
    b>Mcm1 is a multifunctional protein which plays a role both in the initiation of DNA replication and in the transcriptional regulation of diverse genes in Saccharomyces cerevisiae...
  17. Chang V, Fitch M, Donato J, Christensen T, Merchant A, Tye B. Mcm1 binds replication origins. J Biol Chem. 2003;278:6093-100 pubmed
    b>Mcm1 is an essential protein required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes in Saccharomyces cerevisiae...
  18. Shi B. Decoding common and divergent cellular functions of the domains of forkhead transcription factors Fkh1 and Fkh2. Biochem J. 2016;473:3855-3869 pubmed
    ..The result showed that the DBD was vital for ternary complex formation with Mcm1, whereas the FHAD was central for the regulation of cell growth and CLB2 cluster transcription and for interactions ..
  19. Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmed
    ..Thus SKG1 encodes a novel protein that affects the cell wall polymer composition in the growing region of the cell. ..
  20. El Alami M, Messenguy F, Scherens B, Dubois E. Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol. 2003;49:457-68 pubmed
    ..also controls expression of arginine-responsive genes by interacting with Arg80p and Mcm1p, and expression of Mcm1-dependent genes by interacting with Mcm1p...
  21. Lydall D, Ammerer G, Nasmyth K. A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription. Genes Dev. 1991;5:2405-19 pubmed
    ..Two proteins, MCM1, a transcription factor described previously, and SFF (SWI five factor, a newly identified factor) bind this ..
  22. Smith D, Desai A, Johnson A. DNA bending by the a1 and alpha 2 homeodomain proteins from yeast. Nucleic Acids Res. 1995;23:1239-43 pubmed
    ..we found that a dimer of alpha 2 bound to operator DNA produced a mild bend in the DNA, whereas the alpha 2-MCM1-DNA and the a1-alpha 2-DNA complexes exhibited much sharper bends...
  23. Gavin I, Kladde M, Simpson R. Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene. EMBO J. 2000;19:5875-83 pubmed
    ..We suggest that Tup1p can contribute to repression by blocking transcriptional activators, in addition to interacting with transcription machinery and stabilizing chromatin...
  24. Acton T, Zhong H, Vershon A. DNA-binding specificity of Mcm1: operator mutations that alter DNA-bending and transcriptional activities by a MADS box protein. Mol Cell Biol. 1997;17:1881-9 pubmed
    The yeast Mcm1 protein is a member of the MADS box family of transcriptional regulatory factors, a class of DNA-binding proteins found in such diverse organisms as yeast, plants, flies, and humans...
  25. El Bakkoury M, Dubois E, Messenguy F. Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability. Mol Microbiol. 2000;35:15-31 pubmed
    Regulation of arginine metabolism requires the integrity of four regulatory proteins, ArgRI, ArgRII, ArgRIII and Mcm1. To characterize further the interactions between the different proteins, we used the two-hybrid system, which showed ..
  26. Morohashi N, Yamamoto Y, Kuwana S, Morita W, Shindo H, Mitchell A, et al. Effect of sequence-directed nucleosome disruption on cell-type-specific repression by alpha2/Mcm1 in the yeast genome. Eukaryot Cell. 2006;5:1925-33 pubmed
    In Saccharomyces cerevisiae, a-cell-specific genes are repressed in MATalpha cells by alpha2/Mcm1, acting in concert with the Ssn6-Tup1 corepressors and the Isw2 chromatin remodeling complex, and nucleosome positioning has been proposed ..
  27. Hong S, Yoon S. Mcm1p binding sites in the ARG1 promoter positively regulate ARG1 transcription and S. cerevisiae growth in the absence of arginine and Gcn4p. Amino Acids. 2011;40:623-31 pubmed publisher
    ..These findings suggest that the ARG1 promoter utilizes different DNA elements to control transcription, depending on which amino acids are detected in the medium. ..
  28. Pondugula S, Neef D, Voth W, Darst R, Dhasarathy A, Reynolds M, et al. Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins. Mol Cell Biol. 2009;29:4891-905 pubmed publisher
    ..We provide here evidence that Mcm1, a MADS-box protein, is essential for PHO5 mitotic activation...
  29. Spain B, Koo D, Ramakrishnan M, Dzudzor B, Colicelli J. Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit. J Biol Chem. 1995;270:25435-44 pubmed
    ..The properties of Syg1p are consistent with those of a transmembrane signaling component that can respond to, or transduce signals through, G beta or G beta gamma. ..
  30. Lin L, Chamberlain L, Zhu L, Green M. Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4. Proc Natl Acad Sci U S A. 2012;109:1997-2002 pubmed publisher
    ..Finally, we show that although Tra1 is targeted by other activators, these interactions are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction. ..
  31. Carr E, Mead J, Vershon A. Alpha1-induced DNA bending is required for transcriptional activation by the Mcm1-alpha1 complex. Nucleic Acids Res. 2004;32:2298-305 pubmed
    The yeast Mcm1 protein is a founding member of the MADS-box family of transcription factors that is involved in the regulation of diverse sets of genes through interactions with distinct cofactor proteins...
  32. Díaz Blanco N, RODRIGUEZ MEDINA J. Dosage rescue by UBC4 restores cell wall integrity in Saccharomyces cerevisiae lacking the myosin type II gene MYO1. Yeast. 2007;24:343-55 pubmed
    ..These results point to Ubc4p as an important enzyme in the process of cell wall remodelling in myo1 cells. ..
  33. Yoon S, Govind C, Qiu H, Kim S, Dong J, Hinnebusch A. Recruitment of the ArgR/Mcm1p repressor is stimulated by the activator Gcn4p: a self-checking activation mechanism. Proc Natl Acad Sci U S A. 2004;101:11713-8 pubmed
    ..By recruiting an arginine-regulated repressor, Gcn4p can precisely modulate its activation function at ARG1 according to the availability of arginine. ..
  34. Dubois E, Messenguy F. Pleiotropic function of ArgRIIIp (Arg82p), one of the regulators of arginine metabolism in Saccharomyces cerevisiae. Role in expression of cell-type-specific genes. Mol Gen Genet. 1994;243:315-24 pubmed
    ..Western blot analysis indicates that Mcm1p is less abundant in a strain devoid of ArgRIIIp than in wild type. ..
  35. Abraham D, Vershon A. N-terminal arm of Mcm1 is required for transcription of a subset of genes involved in maintenance of the cell wall. Eukaryot Cell. 2005;4:1808-19 pubmed
    The yeast Mcm1 protein is a member of the MADS box family of transcription factors that interacts with several cofactors to differentially regulate genes involved in cell-type determination, mating, cell cycle control and arginine ..
  36. Elble R, Tye B. Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1. Proc Natl Acad Sci U S A. 1991;88:10966-70 pubmed
    b>Mcm1 is a yeast transcription factor with homologs throughout the metazoa. MCM1 was first identified as a gene involved in maintenance of artificial minichromosomes in yeast...
  37. Lim F, Hayes A, West A, Pic Taylor A, Darieva Z, Morgan B, et al. Mcm1p-induced DNA bending regulates the formation of ternary transcription factor complexes. Mol Cell Biol. 2003;23:450-61 pubmed
    ..We constructed mutant mcm1 alleles that are defective in protein-induced bending...
  38. Jamai A, Dubois E, Vershon A, Messenguy F. Swapping functional specificity of a MADS box protein: residues required for Arg80 regulation of arginine metabolism. Mol Cell Biol. 2002;22:5741-52 pubmed
    Arg80 and Mcm1, two members of the MADS box family of DNA-binding proteins, regulate the metabolism of arginine in association with Arg81, the arginine sensor...
  39. Amar N, Messenguy F, El Bakkoury M, Dubois E. ArgRII, a component of the ArgR-Mcm1 complex involved in the control of arginine metabolism in Saccharomyces cerevisiae, is the sensor of arginine. Mol Cell Biol. 2000;20:2087-97 pubmed
    ..Although ArgRI and Mcm1, two MADS-box proteins, and ArgRII, a zinc cluster protein, contain putative DNA binding domains, alone they are ..
  40. Fitch M, Donato J, Tye B. Mcm7, a subunit of the presumptive MCM helicase, modulates its own expression in conjunction with Mcm1. J Biol Chem. 2003;278:25408-16 pubmed
    ..Previous work showed that Mcm1 binds constitutively to the MCM7 promoter and regulates MCM7 expression...
  41. Ma L, Zhai Y, Feng D, Chan T, Lu Y, Fu X, et al. Identification of novel factors involved in or regulating initiation of DNA replication by a genome-wide phenotypic screen in Saccharomyces cerevisiae. Cell Cycle. 2010;9:4399-410 pubmed
    ..These data suggest that Ctf1p and Ctf18p together play important roles in regulating the initiation of DNA replication. ..
  42. Wu C, Weiss K, Yang C, Harris M, Tye B, Newlon C, et al. Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching. Genes Dev. 1998;12:1726-37 pubmed
    ..RE activity is repressed in MATalpha cells by binding of the Matalpha2-Mcm1 corepressor to a site within the RE...
  43. Acton T, Mead J, Steiner A, Vershon A. Scanning mutagenesis of Mcm1: residues required for DNA binding, DNA bending, and transcriptional activation by a MADS-box protein. Mol Cell Biol. 2000;20:1-11 pubmed
    b>MCM1 is an essential gene in the yeast Saccharomyces cerevisiae and is a member of the MADS-box family of transcriptional regulatory factors...