Gene Symbol: MCK1
Description: serine/threonine/tyrosine protein kinase MCK1
Alias: CMS1, YPK1, serine/threonine/tyrosine protein kinase MCK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Shero J, Hieter P. A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1). Genes Dev. 1991;5:549-60 pubmed
    ..This method identified two suppressors, designated MCK1 and CMS2...
  2. Neigeborn L, Mitchell A. The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Genes Dev. 1991;5:533-48 pubmed
    ..Sequence analysis revealed that MCK1 encodes a protein kinase homolog identical to YPK1, a phosphotyrosyl protein with demonstrated protein kinase activity...
  3. Kassir Y, Rubin Bejerano I, Mandel Gutfreund Y. The Saccharomyces cerevisiae GSK-3 beta homologs. Curr Drug Targets. 2006;7:1455-65 pubmed
    Yeast cells carry four homologs of GSK-3beta, RIM11, MCK1, MRK1 and YGK3. The significant homologs are RIM11 and MCK1 that presumably arose from a recent genome duplication followed by a rapid divergence...
  4. Hirata Y, Andoh T, Asahara T, Kikuchi A. Yeast glycogen synthase kinase-3 activates Msn2p-dependent transcription of stress responsive genes. Mol Biol Cell. 2003;14:302-12 pubmed
    The yeast Saccharomyces cerevisiae has four genes, MCK1, MDS1 (RIM11), MRK1, and YOL128c, that encode homologues of mammalian glycogen synthase kinase 3 (GSK-3)...
  5. Puziss J, Hardy T, Johnson R, Roach P, Hieter P. MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase 3. Mol Cell Biol. 1994;14:831-9 pubmed
    The yeast gene MCK1 encodes a serine/threonine protein kinase that is thought to function in regulating kinetochore activity and entry into meiosis...
  6. Ikui A, Cross F. Specific genetic interactions between spindle assembly checkpoint proteins and B-Type cyclins in Saccharomyces cerevisiae. Genetics. 2009;183:51-61 pubmed publisher
    ..Thus, different B-type cyclins are required for distinct aspects of spindle morphogenesis and function, as revealed by differential genetic interactions with spindle checkpoint components. ..
  7. Desany B, Alcasabas A, Bachant J, Elledge S. Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway. Genes Dev. 1998;12:2956-70 pubmed
    ..We propose that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNA replication is stressed. ..
  8. Kishi T, Ikeda A, Nagao R, Koyama N. The SCFCdc4 ubiquitin ligase regulates calcineurin signaling through degradation of phosphorylated Rcn1, an inhibitor of calcineurin. Proc Natl Acad Sci U S A. 2007;104:17418-23 pubmed
    ..The SCF(Cdc4)-dependent degradation required phosphorylation of Rcn1 by Mck1, a member of the GSK3 family of protein kinases, and was promoted by Ca(2+)...
  9. Mizunuma M, Hirata D, Miyaoka R, Miyakawa T. GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down-regulation by Ca2+ in budding yeast. EMBO J. 2001;20:1074-85 pubmed
    ..Our genetic screening identified the MCK1 gene, which encodes a glycogen synthetase kinase-3 family protein kinase, as a component of the Ca2+ signaling ..

More Information


  1. Zimmermann C, Santos A, Gable K, Epstein S, Gururaj C, Chymkowitch P, et al. TORC1 inhibits GSK3-mediated Elo2 phosphorylation to regulate very long chain fatty acid synthesis and autophagy. Cell Rep. 2013;5:1036-46 pubmed publisher
    ..Together, our data reveal a function for TORC1 and GSK3 in the regulation of VLCFA synthesis that has important implications for autophagy and cell homeostasis. ..
  2. Lim M, Dailey D, Martin G, Thorner J. Yeast MCK1 protein kinase autophosphorylates at tyrosine and serine but phosphorylates exogenous substrates at serine and threonine. J Biol Chem. 1993;268:21155-64 pubmed
    The product of the Saccharomyces cerevisiae MCK1 gene is a protein kinase that phosphorylates poly (Glu,Tyr) in vitro and is itself phosphorylated at both tyrosine and serine in vivo...
  3. Sun Y, Miao Y, Yamane Y, Zhang C, Shokat K, Takematsu H, et al. Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Mol Biol Cell. 2012;23:2388-98 pubmed publisher
    ..changes in Orm phosphorylation and sphingoid base production in cells inhibited for yeast protein kinase 1 (Ypk1) activity, that Ypk1 transmits heat stress signals to the sphingolipid biosynthesis pathway via Orm phosphorylation...
  4. Lee J, Moir R, McIntosh K, Willis I. TOR signaling regulates ribosome and tRNA synthesis via LAMMER/Clk and GSK-3 family kinases. Mol Cell. 2012;45:836-43 pubmed publisher
    ..whereupon it primes the phosphorylation of the RNA polymerase III subunit Rpc53 by a specific GSK-3 family member, Mck1. In cooperation with another polymerase subunit, Rpc11, this phosphorylation of Rpc53 modifies the function of the ..
  5. Yu W, Daniel J, Mehta D, Maddipati K, Greenberg M. MCK1 is a novel regulator of myo-inositol phosphate synthase (MIPS) that is required for inhibition of inositol synthesis by the mood stabilizer valproate. PLoS ONE. 2017;12:e0182534 pubmed publisher
    ..The current study identifies Mck1, a GSK3 homolog, as a novel positive regulator of inositol de novo synthesis in yeast...
  6. Andoh T, Hirata Y, Kikuchi A. PY motifs of Rod1 are required for binding to Rsp5 and for drug resistance. FEBS Lett. 2002;525:131-4 pubmed
    ..These results suggest that interaction of Rod1 and Rsp5 is important for drug resistance. ..
  7. Quan Z, Cao L, Tang Y, Yan Y, Oliver S, Zhang N. The Yeast GSK-3 Homologue Mck1 Is a Key Controller of Quiescence Entry and Chronological Lifespan. PLoS Genet. 2015;11:e1005282 pubmed publisher
    ..Here, we identify the yeast GSK-3 homologue Mck1 as a key regulator of G0 entry and reveal that Mck1 acts in parallel to Rim15 to activate starvation-induced gene ..
  8. Huang Z, Chen K, Zhang J, Li Y, Wang H, Cui D, et al. A functional variomics tool for discovering drug-resistance genes and drug targets. Cell Rep. 2013;3:577-85 pubmed publisher
    ..New genes and alleles that confer resistance to other stresses can also be discovered. Similar tools in other systems, such as human cell lines, will also be useful. ..
  9. Xiao Y, Mitchell A. Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p. Mol Cell Biol. 2000;20:5447-53 pubmed
    ..These findings argue that nitrogen limitation governs Rim11p/Mck1p-dependent phosphorylation of Ume6p, which in turn is required for Ume6p-Ime1p interaction and meiotic gene activation. ..
  10. Benni M, Neigeborn L. Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast. Genetics. 1997;147:1351-66 pubmed
    ..Initiation of sporulation is mediated by the meiotic activator IME1, which relies on MCK1 for maximal expression...
  11. Jiang W, Lim M, Yoon H, Thorner J, Martin G, Carbon J. Overexpression of the yeast MCK1 protein kinase suppresses conditional mutations in centromere-binding protein genes CBF2 and CBF5. Mol Gen Genet. 1995;246:360-6 pubmed
    We find that overexpression in yeast of the yeast MCK1 gene, which encodes a meiosis and centromere regulatory kinase, suppresses the temperature-sensitive phenotype of certain mutations in essential centromere binding protein genes CBF2 ..
  12. Rayner T, Gray J, Thorner J. Direct and novel regulation of cAMP-dependent protein kinase by Mck1p, a yeast glycogen synthase kinase-3. J Biol Chem. 2002;277:16814-22 pubmed
    The MCK1 gene of Saccharomyces cerevisiae encodes a protein kinase homologous to metazoan glycogen synthase kinase-3. Previous studies implicated Mck1p in negative regulation of pyruvate kinase...
  13. Cao L, Tang Y, Quan Z, Zhang Z, Oliver S, Zhang N. Chronological Lifespan in Yeast Is Dependent on the Accumulation of Storage Carbohydrates Mediated by Yak1, Mck1 and Rim15 Kinases. PLoS Genet. 2016;12:e1006458 pubmed publisher
    ..Apart from the previously characterised Rim15, Mck1 and Yak1 kinases, the SNF1/AMPK complex, the cell wall integrity pathway and a number of cell cycle regulators were ..
  14. Wilson T. A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics. 2002;162:677-88 pubmed
    ..the postdiauxic/stationary-phase induction of NHEJ that was deficient in other mutants (especially doa1, fyv6, and mck1). These results are discussed in the context of the minimal set of required proteins and regulatory inputs for NHEJ. ..
  15. Brazill D, Thorner J, Martin G. Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. J Bacteriol. 1997;179:4415-8 pubmed
    An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro...
  16. De Silva Udawatta M, Cannon J. Roles of trehalose phosphate synthase in yeast glycogen metabolism and sporulation. Mol Microbiol. 2001;40:1345-56 pubmed
    ..sporulation in tps1 null diploids is caused by reduced expression of meiotic inducers encoded by IME1, IME2 and MCK1. Furthermore, high-copy MCK1 or heterozygous hxk2 mutations can suppress the tps1 sporulation trait...
  17. Searle J, Wood M, Kaur M, Tobin D, Sanchez Y. Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage. PLoS Genet. 2011;7:e1002176 pubmed publisher
    ..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA. ..
  18. Andoh T, Hirata Y, Kikuchi A. Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5. Mol Cell Biol. 2000;20:6712-20 pubmed
    The yeast Saccharomyces cerevisiae has four genes, MCK1, MDS1 (RIM11), MRK1, and YOL128c, that encode glycogen synthase kinase 3 (GSK-3) homologs...
  19. Beeser A, Cooper T. The dual-specificity protein phosphatase Yvh1p acts upstream of the protein kinase mck1p in promoting spore development in Saccharomyces cerevisiae. J Bacteriol. 1999;181:5219-24 pubmed
    ..We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype...
  20. Pal G, Paraz M, Kellogg D. Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1. J Cell Biol. 2008;180:931-45 pubmed publisher
    ..Because casein kinase 1 is associated with sites of polar growth, it may regulate Mih1 as part of a signaling mechanism that links successful completion of growth-related events to cell cycle progression. ..
  21. Lyons N, Fonslow B, Diedrich J, Yates J, Morgan D. Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Nat Struct Mol Biol. 2013;20:194-201 pubmed publisher
    ..degradation requires the sequential actions of Cdk1 and two additional kinases, Cdc7-Dbf4 and the GSK-3 homolog Mck1. These kinases recognize motifs primed by previous phosphorylation, resulting in an ordered sequence of three ..
  22. McQueen J, van Dyk D, Young B, Loewen C, Measday V. The Mck1 GSK-3 kinase inhibits the activity of Clb2-Cdk1 post-nuclear division. Cell Cycle. 2012;11:3421-32 pubmed publisher
    The glycogen synthase kinase-3 homolog, Mck1, has been implicated in many cellular functions, from sporulation to calcium stress response in budding yeast...
  23. Su S, Mitchell A. Identification of functionally related genes that stimulate early meiotic gene expression in yeast. Genetics. 1993;133:67-77 pubmed
    ..We found mutations in IME1, a known positive regultor of IME2, and MCK1, a known positive regulator of IME1...
  24. Ikui A, Rossio V, Schroeder L, Yoshida S. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLoS Genet. 2012;8:e1003099 pubmed publisher
    ..In mck1 deletion cells, CDC6p is stabilized and accumulates in the nucleus even in late S phase and mitosis...
  25. Hwang C, Varshavsky A. Regulation of peptide import through phosphorylation of Ubr1, the ubiquitin ligase of the N-end rule pathway. Proc Natl Acad Sci U S A. 2008;105:19188-93 pubmed publisher
    ..the subsequent (presumably sequential) phosphorylations of Ubr1 on Ser(296), Ser(292), Thr(288), and Tyr(277) by Mck1, a kinase of the glycogen synthase kinase 3 (Gsk3) family...
  26. Al Zain A, Schroeder L, Sheglov A, Ikui A. Cdc6 degradation requires phosphodegron created by GSK-3 and Cdk1 for SCFCdc4 recognition in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:2609-19 pubmed publisher
    ..We previously showed that a yeast GSK-3 homologue, Mck1 kinase, promotes Cdc6 degradation in a SCF(Cdc4)-dependent manner, therefore preventing rereplication...
  27. Drechsler H, Tan A, Liakopoulos D. Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2. J Cell Sci. 2015;128:3910-21 pubmed publisher EB1-binding motif in the N-terminal extension of Kip2 and is negatively regulated by phosphorylation through Mck1, the yeast glycogen synthase kinase 3...
  28. Hermansyah -, LaviƱa W, Sugiyama M, Kaneko Y, Harashima S. Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. Arch Microbiol. 2010;192:157-65 pubmed publisher
  29. Dailey D, Schieven G, Lim M, Marquardt H, Gilmore T, Thorner J, et al. Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity. Mol Cell Biol. 1990;10:6244-56 pubmed
    ..The gene encoding p40 (YPK1) was cloned from a yeast genomic library by using oligonucleotide probes designed on the basis of the sequence of ..
  30. Jiang W, Koltin Y. Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae. Mol Gen Genet. 1996;251:153-60 pubmed
    ..We also provide further in vivo evidence that Mck1p, a protein kinase, is specifically associated with the centromere proteins Cbf2p and Cbf5p, which were previously shown to interact in vitro. ..