Gene Symbol: MCA1
Description: Ca(2+)-dependent cysteine protease MCA1
Alias: YCA1, Ca(2+)-dependent cysteine protease MCA1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bettiga M, Calzari L, Orlandi I, Alberghina L, Vai M. Involvement of the yeast metacaspase Yca1 in ubp10Delta-programmed cell death. FEMS Yeast Res. 2004;5:141-7 pubmed
    ..Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype...
  2. Mazzoni C, Herker E, Palermo V, Jungwirth H, Eisenberg T, Madeo F, et al. Yeast caspase 1 links messenger RNA stability to apoptosis in yeast. EMBO Rep. 2005;6:1076-81 pubmed
    ..Here, we show that mitochondrial function and YCA1, which encodes a budding yeast metacaspase, are necessary for apoptosis triggered by stabilization of mRNAs...
  3. Khan M, Chock P, Stadtman E. Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2005;102:17326-31 pubmed
    ..oxidative stress and apoptosis, we carried out studies with Saccharomyces cerevisiae, which contains a gene (YCA1) that encodes synthesis of metacaspase, a homologue of the mammalian caspase, and is known to play a crucial role ..
  4. Guaragnella N, Bobba A, Passarella S, Marra E, Giannattasio S. Yeast acetic acid-induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1. FEBS Lett. 2010;584:224-8 pubmed publisher
    ..We conclude that alternative death pathways can be activated in yeast AA-PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it.
  5. Madeo F, Herker E, Maldener C, Wissing S, Lächelt S, Herlan M, et al. A caspase-related protease regulates apoptosis in yeast. Mol Cell. 2002;9:911-7 pubmed
    ..We conclude that Yor197w indeed functions as a bona fide caspase in yeast and propose the name Yeast Caspase-1 (YCA1, gene YCA1).
  6. Guaragnella N, Pereira C, Sousa M, Antonacci L, Passarella S, Corte Real M, et al. YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase-like activity. FEBS Lett. 2006;580:6880-4 pubmed
    Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death...
  7. Walter D, Matter A, Fahrenkrog B. Bre1p-mediated histone H2B ubiquitylation regulates apoptosis in Saccharomyces cerevisiae. J Cell Sci. 2010;123:1931-9 pubmed publisher
    ..of Deltabre1 cells depends on the yeast caspase Yca1p, because Deltabre1 cells exhibit increased caspase activity when compared with wild-type cells, and deletion of YCA1 leads to reduced apoptosis sensitivity of cells lacking Bre1p.
  8. Mallela S, Almeida R, Ejsing C, Conzelmann A. Functions of Ceramide Synthase Paralogs YPR114w and YJR116w of Saccharomyces cerevisiae. PLoS ONE. 2016;11:e0145831 pubmed publisher
    ..Ypr114w∆/ypr114w∆ have an increased chronological life span. Thus, Yjr116w and Ypr114w are related, but not functionally redundant. ..
  9. Horowitz A, Lapointe J, Eid R, Sheibani S, Gharib N, Jones N, et al. The human septin7 and the yeast CDC10 septin prevent Bax and copper mediated cell death in yeast. Biochim Biophys Acta. 2013;1833:3186-3194 pubmed publisher
    ..Analysis of septin7 function in a MCA1? yeast strain suggests that septin7 inhibits apoptosis in a caspase independent pathway...

More Information


  1. Szallies A, Kubata B, Duszenko M. A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae. FEBS Lett. 2002;517:144-50 pubmed
    ..GFP-fusion reporters of WWM1, ScMCA1 and TbMCA4 localized to the nucleus. Taken together, we suggest that metacaspases may play a role in nuclear function controlling cellular proliferation coupled to mitochondrial biogenesis...
  2. Lefevre S, Sliwa D, Auchère F, Brossas C, Ruckenstuhl C, Boggetto N, et al. The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells. FEBS Lett. 2012;586:143-8 pubmed publisher
    ..In addition, our results suggest that metacaspase is associated with mitochondrial respiration, intracellular redox control and genomic stability. ..
  3. Lee R, Brunette S, Puente L, Megeney L. Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A. 2010;107:13348-53 pubmed publisher
    ..Here, we report an unexpected role for the Saccharomyces cerevisiae metacaspase Yca1 in protein quality control...
  4. Wong A, Yan C, Shi Y. Crystal structure of the yeast metacaspase Yca1. J Biol Chem. 2012;287:29251-9 pubmed publisher
    b>Yca1, the only metacaspase in Saccharomyces cerevisiae, is thought to be a clan CD cysteine protease that includes the caspase subfamily...
  5. Erhardt M, Wegrzyn R, Deuerling E. Extra N-terminal residues have a profound effect on the aggregation properties of the potential yeast prion protein Mca1. PLoS ONE. 2010;5:e9929 pubmed publisher
    The metacaspase Mca1 from Saccharomyces cerevisiae displays a Q/N-rich region at its N-terminus reminiscent of yeast prion proteins...
  6. Greetham D, Kritsiligkou P, Watkins R, Carter Z, Parkin J, Grant C. Oxidation of the yeast mitochondrial thioredoxin promotes cell death. Antioxid Redox Signal. 2013;18:376-85 pubmed publisher
    ..Apoptosis is dependent on the Yca1 metacaspase, since loss of YCA1 abrogates cell death induced by oxidized Trx3...
  7. Vendrell A, Martínez Pastor M, Gonzalez Novo A, Pascual Ahuir A, Sinclair D, Proft M, et al. Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase. EMBO Rep. 2011;12:1062-8 pubmed publisher
    ..Sir2 is involved in protection against Hog1-induced cell death and can suppress Hog1-induced ROS accumulation. Therefore, cell death seems to be dictated by the balance of ROS induced by Hog1 and the protective effect of Sir2. ..
  8. Lee Y, Hoe K, Maeng P. Yeast cells lacking the CIT1-encoded mitochondrial citrate synthase are hypersusceptible to heat- or aging-induced apoptosis. Mol Biol Cell. 2007;18:3556-67 pubmed
    ..Activation of the metacaspase Yca1 was detected during heat- or aging-induced apoptosis in cit1 null strains, and accordingly, deletion of YCA1 ..
  9. Longo V, Ždralević M, Guaragnella N, Giannattasio S, Zolla L, Timperio A. Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death. J Proteomics. 2015;128:173-88 pubmed publisher acetic acid (AA-PCD) in Saccharomyces cerevisiae both in the presence and absence of metacaspase encoding gene YCA1. Here, we report an unexpected role for the yeast metacaspase in protein quality and metabolite control...
  10. Orlandi I, Casatta N, Vai M. Lack of Ach1 CoA-Transferase Triggers Apoptosis and Decreases Chronological Lifespan in Yeast. Front Oncol. 2012;2:67 pubmed publisher
  11. Shrestha A, Puente L, Brunette S, Megeney L. The role of Yca1 in proteostasis. Yca1 regulates the composition of the insoluble proteome. J Proteomics. 2013;81:24-30 pubmed publisher
    ..We have previously implicated the Saccharomyces cerevisiae metacaspase Yca1 as an active participant in maintaining proteostasis, whereby Yca1 acts to limit aggregate content...
  12. Petitjean M, Teste M, Leger Silvestre I, Francois J, Parrou J. A new function for the yeast trehalose-6P synthase (Tps1) protein, as key pro-survival factor during growth, chronological ageing, and apoptotic stress. Mech Ageing Dev. 2017;161:234-246 pubmed publisher
    ..Genetic interaction analysis of TPS1 and genes such as YCA1, NUC1 and AIF1 indicated that these key executioners of cell death partially relayed tps1?-triggered signaling...
  13. Kajiwara K, Muneoka T, Watanabe Y, Karashima T, Kitagaki H, Funato K. Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast. Mol Microbiol. 2012;86:1246-61 pubmed publisher
  14. Johansson M, Chen X, Milanova S, Santos C, Petranovic D. PUFA-induced cell death is mediated by Yca1p-dependent and -independent pathways, and is reduced by vitamin C in yeast. FEMS Yeast Res. 2016;16:fow007 pubmed publisher
    ..When deleting YCA1, the caspase-like activity and the oxidative stress decreased and although the lifespan was slightly prolonged, the ..
  15. Almeida T, Marques M, Mojzita D, Amorim M, Silva R, Almeida B, et al. Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosis. Mol Biol Cell. 2008;19:865-76 pubmed
    ..These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis. ..
  16. Walter D, Matter A, Fahrenkrog B. Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004095 pubmed publisher
    ..cerevisiae. Given the evolutionary conservation of H3K4 methylation this likely plays a role in apoptosis regulation in a wide range of organisms...
  17. Lisa Santamaría P, Neiman A, Cuesta Marbán A, Mollinedo F, Revuelta J, Jimenez A. Human initiator caspases trigger apoptotic and autophagic phenotypes in Saccharomyces cerevisiae. Biochim Biophys Acta. 2009;1793:561-71 pubmed publisher
    ..Furthermore, caspase-induced cell death was completely dependent on the proteolytic activation of the enzyme but, in contrast, was not dependent on either of the endogenous yeast apoptotic proteins Aif1 and Mca1 or the mitochondria.
  18. Rinnerthaler M, Buttner S, Laun P, Heeren G, Felder T, Klinger H, et al. Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast. Proc Natl Acad Sci U S A. 2012;109:8658-63 pubmed publisher
    ..We renamed this ORF YNO1 (yeast NADPH oxidase 1). Overexpression of YNO1 causes YCA1-dependent apoptosis, whereas deletion of the gene makes cells less sensitive to apoptotic stimuli...
  19. Lee R, Puente L, Kaern M, Megeney L. A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics. PLoS ONE. 2008;3:e2956 pubmed publisher
    ..S. cerevisiae expresses a primordial caspase, yca1, and exhibits apoptosis-like death under certain stresses; however, the benefit of a dedicated death program to ..
  20. Cui Y, Zhao S, Wu Z, Dai P, Zhou B. Mitochondrial release of the NADH dehydrogenase Ndi1 induces apoptosis in yeast. Mol Biol Cell. 2012;23:4373-82 pubmed publisher
  21. Ždralević M, Longo V, Guaragnella N, Giannattasio S, Timperio A, Zolla L. Differential proteome-metabolome profiling of YCA1-knock-out and wild type cells reveals novel metabolic pathways and cellular processes dependent on the yeast metacaspase. Mol Biosyst. 2015;11:1573-83 pubmed publisher
    The yeast Saccharomyces cerevisiae expresses one member of the metacaspase Cys protease family, encoded by the YCA1 gene...