MAF1

Summary

Gene Symbol: MAF1
Description: RNA polymerase III-inhibiting protein MAF1
Alias: RNA polymerase III-inhibiting protein MAF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Upadhya R, Lee J, Willis I. Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription. Mol Cell. 2002;10:1489-94 pubmed
    b>Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S...
  2. Cieśla M, Towpik J, Graczyk D, Oficjalska Pham D, Harismendy O, Suleau A, et al. Maf1 is involved in coupling carbon metabolism to RNA polymerase III transcription. Mol Cell Biol. 2007;27:7693-702 pubmed
    ..In the yeast Saccharomyces cerevisiae, Maf1 is the only known global and direct Pol III transcription repressor which mediates numerous stress signals...
  3. Towpik J, Graczyk D, Gajda A, Lefebvre O, Boguta M. Derepression of RNA polymerase III transcription by phosphorylation and nuclear export of its negative regulator, Maf1. J Biol Chem. 2008;283:17168-74 pubmed publisher
    b>Maf1 is the global repressor of RNA polymerase III (Pol III) in yeast Saccharomyces cerevisiae. Transcription regulation by Maf1 is important under stress conditions and during the switch between fermentation and respiration...
  4. Lee J, Moir R, Willis I. Regulation of RNA polymerase III transcription involves SCH9-dependent and SCH9-independent branches of the target of rapamycin (TOR) pathway. J Biol Chem. 2009;284:12604-8 pubmed publisher
    b>Maf1 is a conserved repressor of transcription that functions at the downstream end of multiple nutrient and stress signaling pathways. How these different signaling pathways converge on Maf1 is not known...
  5. Boisnard S, Lagniel G, Garmendia Torres C, Molin M, Boy Marcotte E, Jacquet M, et al. H2O2 activates the nuclear localization of Msn2 and Maf1 through thioredoxins in Saccharomyces cerevisiae. Eukaryot Cell. 2009;8:1429-38 pubmed publisher
    ..specific to oxidative stress, and others, such as the transcriptional activators Msn2/4 and the negative regulator Maf1, are activated by a wide spectrum of stress conditions...
  6. Huber A, Bodenmiller B, Uotila A, Stahl M, Wanka S, Gerrits B, et al. Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev. 2009;23:1929-43 pubmed publisher
    ..Follow-up detailed characterization shows that Sch9 regulates RNA polymerases I and III, the latter via Maf1, in addition to translation initiation and the expression of ribosomal protein and ribosome biogenesis genes...
  7. Karkusiewicz I, Turowski T, Graczyk D, Towpik J, Dhungel N, Hopper A, et al. Maf1 protein, repressor of RNA polymerase III, indirectly affects tRNA processing. J Biol Chem. 2011;286:39478-88 pubmed publisher
    b>Maf1 is negative regulator of RNA polymerase III in yeast. We observed high levels of both primary transcript and end-matured, intron-containing pre-tRNAs in the maf1? strain...
  8. Desai N, Lee J, Upadhya R, Chu Y, Moir R, Willis I. Two steps in Maf1-dependent repression of transcription by RNA polymerase III. J Biol Chem. 2005;280:6455-62 pubmed
    In Saccharomyces cerevisiae, Maf1 is essential for mediating the repression of transcription by RNA polymerase (pol) III in response to diverse cellular conditions...
  9. Oficjalska Pham D, Harismendy O, Smagowicz W, Gonzalez de Peredo A, Boguta M, Sentenac A, et al. General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1. Mol Cell. 2006;22:623-32 pubmed
    We report genome-wide analyses that establish Maf1 as a general and direct repressor of yeast RNA polymerase (Pol) III transcription...

More Information

Publications28

  1. Vannini A, Ringel R, Kusser A, Berninghausen O, Kassavetis G, Cramer P. Molecular basis of RNA polymerase III transcription repression by Maf1. Cell. 2010;143:59-70 pubmed publisher
    ..Under stress conditions, the conserved protein Maf1 rapidly represses Pol III transcription...
  2. Wei Y, Tsang C, Zheng X. Mechanisms of regulation of RNA polymerase III-dependent transcription by TORC1. EMBO J. 2009;28:2220-30 pubmed publisher
    ..Pol) III, as well as the phosphorylation and binding to Pol III-transcribed genes of the Pol III repressor Maf1. Interestingly, TORC1 does not bind to tRNA genes, suggesting that TORC1 modulates tRNA synthesis indirectly ..
  3. Pluta K, Lefebvre O, Martin N, Smagowicz W, Stanford D, Ellis S, et al. Maf1p, a negative effector of RNA polymerase III in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:5031-40 pubmed
    ..Previous studies identified MAF1, a gene that affects tRNA suppressor efficiency and interacts genetically with Pol III...
  4. Gajda A, Towpik J, Steuerwald U, Müller C, Lefebvre O, Boguta M. Full repression of RNA polymerase III transcription requires interaction between two domains of its negative regulator Maf1. J Biol Chem. 2010;285:35719-27 pubmed publisher
    b>Maf1, first identified in yeast Saccharomyces cerevisiae, is a general negative regulator of RNA polymerase III (Pol III)...
  5. Roberts D, Wilson B, Huff J, Stewart A, Cairns B. Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression. Mol Cell. 2006;22:633-44 pubmed
    ..cerevisiae. The signaling pathways that relay stress and nutrient conditions converge on the conserved protein Maf1, but how Maf1 integrates environmental conditions and couples them to transcriptional repression is largely unknown...
  6. Graczyk D, Debski J, Muszynska G, Bretner M, Lefebvre O, Boguta M. Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation. Proc Natl Acad Sci U S A. 2011;108:4926-31 pubmed publisher
    b>Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man...
  7. Morawiec E, Wichtowska D, Graczyk D, Conesa C, Lefebvre O, Boguta M. Maf1, repressor of tRNA transcription, is involved in the control of gluconeogenetic genes in Saccharomyces cerevisiae. Gene. 2013;526:16-22 pubmed publisher
    b>Maf1 is a negative regulator of RNA polymerase III (Pol III) in yeast. Maf1-depleted cells manifest elevated tRNA transcription and inability to grow on non-fermentable carbon source, such as glycerol...
  8. Wei Y, Zheng X. Sch9 partially mediates TORC1 signaling to control ribosomal RNA synthesis. Cell Cycle. 2009;8:4085-90 pubmed
    ..Mechanistically, Sch9 is involved in hyperphosphorylation and cytoplasmic localization of Maf1, and for optimal synthesis of rRNAs and tRNAs...
  9. Moir R, Lee J, Haeusler R, Desai N, Engelke D, Willis I. Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1. Proc Natl Acad Sci U S A. 2006;103:15044-9 pubmed
    b>Maf1 is an essential and specific mediator of transcriptional repression in the RNA polymerase (pol) III system...
  10. Boguta M, Czerska K, Zoładek T. Mutation in a new gene MAF1 affects tRNA suppressor efficiency in Saccharomyces cerevisiae. Gene. 1997;185:291-6 pubmed
    Mutation in the MAF1 gene was identified in a screen for decreased efficiency of tRNA suppressor SUP11 in the yeast Saccharomyces cerevisiae (Sc)...
  11. Oler A, Cairns B. PP4 dephosphorylates Maf1 to couple multiple stress conditions to RNA polymerase III repression. EMBO J. 2012;31:1440-52 pubmed publisher
    b>Maf1 is the 'master' repressor of RNA polymerase III (Pol III) transcription in yeast, and is conserved in eukaryotes...
  12. Moir R, Lee J, Willis I. Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation. J Biol Chem. 2012;287:30833-41 pubmed publisher
    b>Maf1 is a conserved regulator of RNA polymerase (pol) III transcription and is required for transcriptional repression under diverse stress conditions...
  13. Turowski T, Karkusiewicz I, Kowal J, Boguta M. Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway. RNA. 2012;18:1823-32 pubmed publisher
    ..We also identified MAF1 whose product is a general negative regulator of RNA polymerase III...
  14. Acker J, Nguyen N, Vandamme M, Tavenet A, Briand Suleau A, Conesa C. Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle. PLoS ONE. 2014;9:e114587 pubmed publisher
    Sub1 and Maf1 exert an opposite effect on RNA polymerase III transcription interfering with different steps of the transcription cycle...
  15. Lee J, Moir R, Willis I. Differential Phosphorylation of RNA Polymerase III and the Initiation Factor TFIIIB in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0127225 pubmed publisher
    ..activity directly or indirectly, through downstream kinases, alter the phosphorylation state and function of the Maf1 repressor and Rpc53, a TFIIF-like subunit of the polymerase...
  16. Foretek D, Nuc P, Zywicki M, Karlowski W, Kudla G, Boguta M. Maf1-mediated regulation of yeast RNA polymerase III is correlated with CCA addition at the 3' end of tRNA precursors. Gene. 2017;612:12-18 pubmed publisher
    In eukaryotic cells tRNA synthesis is negatively regulated by the protein Maf1, conserved from yeast to humans...
  17. Good P, Kendall A, Ignatz Hoover J, Miller E, Pai D, Rivera S, et al. Silencing near tRNA genes is nucleosome-mediated and distinct from boundary element function. Gene. 2013;526:7-15 pubmed publisher
    ..Models for communication between the tRNA gene transcription complexes and local chromatin are discussed. ..
  18. Cieśla M, Makała E, Płonka M, Bazan R, Gewartowski K, Dziembowski A, et al. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae. Mol Cell Biol. 2015;35:1169-81 pubmed publisher
    ..Additionally, Rbs1 interacts with the Crm1 exportin and shuttles between the cytoplasm and nucleus. We postulate that Rbs1 binds to the Pol III complex or subcomplex and facilitates its translocation to the nucleus. ..
  19. Cai Y, Wei Y. Distinct regulation of Maf1 for lifespan extension by Protein kinase A and Sch9. Aging (Albany NY). 2015;7:133-43 pubmed
    The Protein kinase A (PKA) and Sch9 regulates cell growth as well as lifespan in Saccharomyces cerevisiae. Maf1 is a RNA polymerase III (PolIII) inhibitor that tailors 5S rRNA and tRNA production in response to various environmental cues...