LST8

Summary

Gene Symbol: LST8
Description: TOR complex subunit LST8
Alias: TOR complex subunit LST8
Species: Saccharomyces cerevisiae S288c
Products:     LST8

Top Publications

  1. Roberg K, Bickel S, Rowley N, Kaiser C. Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8. Genetics. 1997;147:1569-84 pubmed
    ..Three new genes, LST4, LST7, and LST8, were identified that are also required for amino acid permease transport from the Golgi to the cell surface...
  2. Liu Z, Sekito T, Epstein C, Butow R. RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p. EMBO J. 2001;20:7209-19 pubmed
    ..Here we report that some mutations [lst8-(2-5)] in LST8, an essential gene encoding a seven WD40-repeat protein required for targeting of amino acid ..
  3. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed
    ..TOR Complex 1 (TORC1) contains TOR1 or TOR2, KOG1 (YHR186c), and LST8. TORC2 contains TOR2, AVO1 (YOL078w), AVO2 (YMR068w), AVO3 (YER093c), and LST8...
  4. Reinke A, Anderson S, McCaffery J, Yates J, Aronova S, Chu S, et al. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem. 2004;279:14752-62 pubmed
    ..Together, these results define a novel connection between TORC1 and Ssd1p-mediated maintenance of cellular integrity. ..
  5. Singh J, Tyers M. A Rab escort protein integrates the secretion system with TOR signaling and ribosome biogenesis. Genes Dev. 2009;23:1944-58 pubmed publisher
    ..The Sfp1-Mrs6 interaction is a nexus for growth regulation that links the secretory system and TOR-dependent nutrient signaling to ribosome biogenesis. ..
  6. Pracheil T, Thornton J, Liu Z. TORC2 signaling is antagonized by protein phosphatase 2A and the Far complex in Saccharomyces cerevisiae. Genetics. 2012;190:1325-39 pubmed publisher
    ..b>Lst8, a component of both TOR complexes, is essential for cell viability...
  7. Giannattasio S, Liu Z, Thornton J, Butow R. Retrograde response to mitochondrial dysfunction is separable from TOR1/2 regulation of retrograde gene expression. J Biol Chem. 2005;280:42528-35 pubmed
  8. Wanke V, Cameroni E, Uotila A, Piccolis M, Urban J, Loewith R, et al. Caffeine extends yeast lifespan by targeting TORC1. Mol Microbiol. 2008;69:277-85 pubmed publisher
    ..This kinase cascade appears to have been evolutionarily conserved, suggesting that caffeine may extend lifespan in other eukaryotes, including man. ..
  9. Lempiäinen H, Uotila A, Urban J, Dohnal I, Ammerer G, Loewith R, et al. Sfp1 interaction with TORC1 and Mrs6 reveals feedback regulation on TOR signaling. Mol Cell. 2009;33:704-16 pubmed publisher
    ..Finally, we show that the Sfp1-interacting protein Mrs6, a Rab escort protein involved in membrane trafficking, regulates both Sfp1 nuclear localization and TORC1 signaling. ..

More Information

Publications23

  1. Sekiguchi T, Hayashi N, Wang Y, Kobayashi H. Genetic evidence that Ras-like GTPases, Gtr1p, and Gtr2p, are involved in epigenetic control of gene expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2008;368:748-54 pubmed publisher
    ..We propose that Gtr1p and Gtr2p are involved in epigenetic control of gene expression in the TOR signaling pathway. ..
  2. Baretić D, Berndt A, Ohashi Y, Johnson C, Williams R. Tor forms a dimer through an N-terminal helical solenoid with a complex topology. Nat Commun. 2016;7:11016 pubmed publisher
    ..Tor is present in two complexes, TORC1 and TORC2, in which the Tor-Lst8 heterodimer forms a common sub-complex...
  3. Gaubitz C, Oliveira T, Prouteau M, Leitner A, Karuppasamy M, Konstantinidou G, et al. Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2. Mol Cell. 2015;58:977-88 pubmed publisher
    ..Removal of this sequence generated a FKBP12-rapamycin-sensitive TORC2 variant, which provides a powerful tool for deciphering TORC2 function in vivo. Using this variant, we demonstrate a role for TORC2 in G2/M cell-cycle progression. ..
  4. Kingsbury J, Sen N, Maeda T, Heitman J, Cardenas M. Endolysosomal membrane trafficking complexes drive nutrient-dependent TORC1 signaling to control cell growth in Saccharomyces cerevisiae. Genetics. 2014;196:1077-89 pubmed publisher
  5. Yan Y, Kang B. Regulation of Vid-dependent degradation of FBPase by TCO89, a component of TOR Complex 1. Int J Biol Sci. 2010;6:361-70 pubmed
    ..The P1S mutation of FBPase, reported to block its degradation, was observed to impair the association of FBPase with TORCI components. These results implicated an important regulatory role of TCO89 and TORCI in this pathway. ..
  6. Devasahayam G, Ritz D, Helliwell S, Burke D, Sturgill T. Pmr1, a Golgi Ca2+/Mn2+-ATPase, is a regulator of the target of rapamycin (TOR) signaling pathway in yeast. Proc Natl Acad Sci U S A. 2006;103:17840-5 pubmed
    ..Epistasis analyses show that Pmr1 functions upstream from Npr1 and Gln-3 in opposition to Lst8, a regulator of TOR...
  7. Wullschleger S, Loewith R, Oppliger W, Hall M. Molecular organization of target of rapamycin complex 2. J Biol Chem. 2005;280:30697-704 pubmed
    ..through a rapamycin-insensitive signaling branch and in yeast consists of the six proteins AVO1, AVO2, AVO3, BIT61, LST8, and TOR2. Here we have focused on the characterization of TORC2...
  8. Wedaman K, Reinke A, Anderson S, Yates J, McCaffery J, Powers T. Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:1204-20 pubmed
    ..Cell fractionation, coimmunoprecipitation, and immunogold electron microscopy experiments confirmed that Lst8 associates with both Tor2p as well as Tor1p at these membranous sites...
  9. Roberg K, Crotwell M, Espenshade P, Gimeno R, Kaiser C. LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum. J Cell Biol. 1999;145:659-72 pubmed
    ..Together, these findings suggest that a specialized form of the COPII coat subunit, with Lst1p in place of Sec24p, is used for the efficient packaging of Pma1p into vesicles derived from the ER. ..
  10. Liao H, Chen M. Target of rapamycin complex 2 signals to downstream effector yeast protein kinase 2 (Ypk2) through adheres-voraciously-to-target-of-rapamycin-2 protein 1 (Avo1) in Saccharomyces cerevisiae. J Biol Chem. 2012;287:6089-99 pubmed publisher
    ..Together, our data support the conclusion that the direct Avo1-Ypk2 interaction is crucial for TORC2 signaling to the downstream Ypk2 pathway. ..
  11. Kabir M, Kaminska J, Segel G, Bethlendy G, Lin P, Della Seta F, et al. Physiological effects of unassembled chaperonin Cct subunits in the yeast Saccharomyces cerevisiae. Yeast. 2005;22:219-39 pubmed
    ..a wide range of abnormal phenotypes, including those caused by the diverse types of conditional mutations tor2-21, lst8-2 and rsp5-9 and those caused by the concomitant overexpression of Sit4p and Sap155p...
  12. Chen E, Kaiser C. LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway. J Cell Biol. 2003;161:333-47 pubmed
    b>LST8, a Saccharomyces cerevisiae gene encoding a 34-kD WD-repeat protein, was identified by mutations that caused defects in sorting Gap1p to the plasma membrane...
  13. Brown C, Hung G, Dunton D, Chiang H. The TOR complex 1 is distributed in endosomes and in retrograde vesicles that form from the vacuole membrane and plays an important role in the vacuole import and degradation pathway. J Biol Chem. 2010;285:23359-70 pubmed publisher
    ..We suggest that TORC1 interacts with multiple cargo proteins destined for the Vid pathway and plays an important role in the degradation of FBPase in the vacuole. ..
  14. Ho H, Lee H, Liao H, Chen M. Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Eukaryot Cell. 2008;7:1328-43 pubmed publisher
    ..Our results also suggest that Avo2p/Slm1p-mediated signaling, but not Avo1p-mediated signaling, links to Rho1p activation specifically through the Rho1p-guanine nucleotide exchange factor Tus1p. ..