LSM6

Summary

Gene Symbol: LSM6
Description: U4/U6-U5 snRNP complex subunit LSM6
Alias: U4/U6-U5 snRNP complex subunit LSM6
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Tharun S, He W, Mayes A, Lennertz P, Beggs J, Parker R. Yeast Sm-like proteins function in mRNA decapping and decay. Nature. 2000;404:515-8 pubmed
    ..In addition, the Lsm complex that functions in mRNA decay appears to be distinct from the U6-associated Lsm complex, indicating that Lsm proteins form specific complexes that affect different aspects of mRNA metabolism. ..
  2. Pannone B, Kim S, Noe D, Wolin S. Multiple functional interactions between components of the Lsm2-Lsm8 complex, U6 snRNA, and the yeast La protein. Genetics. 2001;158:187-96 pubmed
    ..Furthermore, deletions of LSM5, LSM6, or LSM7 cause LHP1 to become required for growth...
  3. Mayes A, Verdone L, Legrain P, Beggs J. Characterization of Sm-like proteins in yeast and their association with U6 snRNA. EMBO J. 1999;18:4321-31 pubmed
    ..We discuss the possible existence and functions of alternative Lsm complexes, including the likelihood that they are involved in processes other than pre-mRNA splicing. ..
  4. Naidoo N, Harrop S, Sobti M, Haynes P, Szymczyna B, Williamson J, et al. Crystal structure of Lsm3 octamer from Saccharomyces cerevisiae: implications for Lsm ring organisation and recruitment. J Mol Biol. 2008;377:1357-71 pubmed publisher
    ..The homomeric Lsm3 octamer is found to successfully recruit Lsm6, Lsm2 and Lsm5 directly from yeast lysate...
  5. Salgado Garrido J, Bragado Nilsson E, Kandels Lewis S, Seraphin B. Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J. 1999;18:3451-62 pubmed
    ..We also identified archaeal proteins related to Sm and Sm-like proteins. Our results demonstrate that Sm and Sm-like proteins assemble in at least two functionally conserved complexes of deep evolutionary origin. ..
  6. Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
    ..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
  7. Chowdhury A, Mukhopadhyay J, Tharun S. The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs. RNA. 2007;13:998-1016 pubmed
    ..These results indicate that the intrinsic RNA-binding characteristics of this complex form a critical determinant of its in vivo interactions and functions. ..
  8. Didychuk A, Montemayor E, Carrocci T, DeLaitsch A, Lucarelli S, Westler W, et al. Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities. Nat Commun. 2017;8:497 pubmed publisher
    ..Here the authors characterize the enzymatic activities and structures of yeast and human U6 RNA processing enzyme Usb1, reconstitute post-transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembly. ..
  9. Chowdhury A, Raju K, Kalurupalle S, Tharun S. Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex. RNA. 2012;18:936-44 pubmed publisher
    ..Thus, unlike most Sm-like proteins, Lsm1 uniquely requires both its Sm-domain and CTD for its normal RNA-binding function. ..

More Information

Publications25

  1. Luo X, Talarek N, De Virgilio C. Initiation of the yeast G0 program requires Igo1 and Igo2, which antagonize activation of decapping of specific nutrient-regulated mRNAs. RNA Biol. 2011;8:14-7 pubmed
    ..Accordingly, Igo1/2 likely play a key role in preventing the decapping and subsequent 5'-3' degradation of a set of nutrient-regulated mRNAs that are critical for cell differentiation and chronological life span. ..
  2. Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
    ..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation. ..
  3. Fernandez C, Pannone B, Chen X, Fuchs G, Wolin S. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Mol Biol Cell. 2004;15:2842-52 pubmed
  4. Reijns M, Alexander R, Spiller M, Beggs J. A role for Q/N-rich aggregation-prone regions in P-body localization. J Cell Sci. 2008;121:2463-72 pubmed publisher
    ..This is supported by reduced P-body accumulation of Ccr4p, Pop2p and Dhh1p after deletion of these domains, and by the observed aggregation of the Q/N-rich region from Ccr4p. ..
  5. Chowdhury A, Tharun S. lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo. RNA. 2008;14:2149-58 pubmed publisher
    ..These results underscore the importance of Lsm1p-7p-Pat1p complex-mRNA interaction for mRNA decay in vivo and imply that the oligo(A) tail mediated enhancement of such interaction is crucial in that process. ..
  6. Tharun S. Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast. Methods Enzymol. 2008;448:41-55 pubmed publisher
    ..2007). These observations, therefore, highlighted the importance of the intrinsic RNA binding properties of this complex as key determinants of its in vivo functions. ..
  7. Sobti M, Cubeddu L, Haynes P, Mabbutt B. Engineered rings of mixed yeast Lsm proteins show differential interactions with translation factors and U-rich RNA. Biochemistry. 2010;49:2335-45 pubmed publisher
    ..Our findings suggest Lsm1 and/or Lsm4 can interact with translationally active mRNA. ..
  8. Sharif H, Conti E. Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover. Cell Rep. 2013;5:283-91 pubmed publisher
    ..These results show how the auxiliary domains and the canonical Sm folds of the Lsm1-7 complex are organized in order to mediate and modulate macromolecular interactions. ..
  9. Vidal V, Verdone L, Mayes A, Beggs J. Characterization of U6 snRNA-protein interactions. RNA. 1999;5:1470-81 pubmed
    ..Interestingly, the Lsm proteins associate efficiently with the 3' half of U6, which contains the 3' stem-loop and uridine-rich 3' end, suggesting that the Lsm and Sm proteins may recognize similar features in RNAs. ..
  10. Spiller M, Reijns M, Beggs J. Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes. J Cell Sci. 2007;120:4310-20 pubmed
    ..A shift of Lsm proteins from the nucleus to the cytoplasm under stress conditions indicates that this competition is biologically significant. ..
  11. Karaduman R, Dube P, Stark H, Fabrizio P, Kastner B, Luhrmann R. Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy. RNA. 2008;14:2528-37 pubmed publisher
    ..Further, LSmp-Prp24p interactions may be restricted to the closed form, which appears to represent the solution structure of the U6 snRNP particle. ..
  12. Li Z, Lee I, Moradi E, Hung N, Johnson A, Marcotte E. Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol. 2009;7:e1000213 pubmed publisher
  13. Boon K, Norman C, Grainger R, Newman A, Beggs J. Prp8p dissection reveals domain structure and protein interaction sites. RNA. 2006;12:198-205 pubmed
    ..Thus, the U5 snRNP protein Snu114p associates with Prp8p in the region 437-770, whereas fragmenting Prp8p at residue 2173 destabilizes its association with Aar2p. ..
  14. Karaduman R, Fabrizio P, Hartmuth K, Urlaub H, Luhrmann R. RNA structure and RNA-protein interactions in purified yeast U6 snRNPs. J Mol Biol. 2006;356:1248-62 pubmed
    ..Interestingly, we find that the open structure of the yeast U6 snRNA in native snRNPs can also be adopted by human U6 and U6atac snRNAs. ..
  15. Cuenca Bono B, García Molinero V, Pascual García P, García Oliver E, Llopis A, Rodriguez Navarro S. A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolism. BMC Cell Biol. 2010;11:19 pubmed publisher
    ..evidence for genetic interactions between SUS1 and genes coding for components of P-bodies such as PAT1, LSM1, LSM6 and DHH1...
  16. Wu D, Muhlrad D, Bowler M, Jiang S, Liu Z, Parker R, et al. Lsm2 and Lsm3 bridge the interaction of the Lsm1-7 complex with Pat1 for decapping activation. Cell Res. 2014;24:233-46 pubmed publisher
    ..Based on the structure of Lsm2-3-Pat1C, a model of Lsm1-7-Pat1 complex is constructed and how RNA binds to this complex is discussed. ..