Gene Symbol: LEU2
Description: 3-isopropylmalate dehydrogenase
Alias: 3-isopropylmalate dehydrogenase
Species: Saccharomyces cerevisiae S288c
Products:     LEU2

Top Publications

  1. Kohlhaw G. Beta-isopropylmalate dehydrogenase from yeast. Methods Enzymol. 1988;166:429-35 pubmed
  2. Klasson H, Fink G, Ljungdahl P. Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids. Mol Cell Biol. 1999;19:5405-16 pubmed
    ..These findings suggest that Ssy1p and Ptr3p function in the PM as components of a sensor of extracellular amino acids. ..
  3. Ryan E, Tracy J, Kohlhaw G. Subcellular localization of the leucine biosynthetic enzymes in yeast. J Bacteriol. 1973;116:222-5 pubmed
  4. Hueso G, Aparicio Sanchis R, Montesinos C, Lorenz S, Murguía J, Serrano R. A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress. Biochem J. 2012;441:255-64 pubmed publisher
    ..One corresponded to LEU2 and functions by removing the dependency of the leu2 mutant host strain on uptake of extracellular leucine...
  5. Andreadis A, Hsu Y, Kohlhaw G, Schimmel P. Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine. Cell. 1982;31:319-25 pubmed
    The LEU2 structural gene and its regulatory sequences were isolated on a 2200 bp Xho I-Sal I fragment. Sequencing of the 5'-noncoding region showed that at -151 there is an open reading frame of 23 codons of which six are for leucine...
  6. Nigavekar S, Cannon J. Characterization of genes that are synthetically lethal with ade3 or leu2 in Saccharomyces cerevisiae. Yeast. 2002;19:115-22 pubmed
    ..Using a LEU2-ADE3 plasmid, plasmid-dependent mutations were found in the SHM2, PTR3, BAP2 and SSY1 genes...
  7. López Mirabal H, Winther J, Kielland Brandt M. Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2007;71:2934-42 pubmed
    ..Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant...
  8. Li Z, Vizeacoumar F, Bahr S, Li J, Warringer J, Vizeacoumar F, et al. Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Nat Biotechnol. 2011;29:361-7 pubmed publisher
    ..This mutant collection should facilitate a wide range of systematic studies aimed at understanding the functions of essential genes. ..
  9. Ano A, Suehiro D, Cha aim K, Aritomi K, Phonimdaeng P, Nontaso N, et al. Combinatorial gene overexpression and recessive mutant gene introduction in sake yeast. Biosci Biotechnol Biochem. 2009;73:633-40 pubmed
    ..Moreover, none of these sake strains had extraneous sequences, and they are thus suitable for use in commercial applications. ..

More Information


  1. Bedekovics T, Li H, Gajdos G, Isaya G. Leucine biosynthesis regulates cytoplasmic iron-sulfur enzyme biogenesis in an Atm1p-independent manner. J Biol Chem. 2011;286:40878-88 pubmed publisher
    ..Enzymatic blocks in the pathway (i.e. leu1? or leu2? gene deletion mutations) induced post-transcriptional up-regulation of core components of mitochondrial ISC ..
  2. Chang C, Clarke A, Pillus L. Suppression analysis of esa1 mutants in Saccharomyces cerevisiae links NAB3 to transcriptional silencing and nucleolar functions. G3 (Bethesda). 2012;2:1223-32 pubmed publisher
    ..In this study, four genes (LYS20, LEU2, VAP1, and NAB3) were identified in a genetic screen as high-copy suppressors of the conditional temperature-..
  3. Merhi A, Gérard N, Lauwers E, Prevost M, Andre B. Systematic mutational analysis of the intracellular regions of yeast Gap1 permease. PLoS ONE. 2011;6:e18457 pubmed publisher
    ..Interestingly, this direct vacuolar sorting seems to be independent of Gap1 ubiquitylation. This study illustrates the importance of multiple intracellular regions of Gap1 in its secretion, transport activity, and down-regulation. ..
  4. Branduardi P, Longo V, Berterame N, Rossi G, Porro D. A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae. Biotechnol Biofuels. 2013;6:68 pubmed publisher
    ..cerevisiae, using glycine as a substrate. Doors are now opened for a number of optimizations, also considering that starting from an aminoacid mixture as a side stream process, a fusel alcohol blend can be generated. ..