LEA1

Summary

Gene Symbol: LEA1
Description: U2 snRNP complex subunit LEA1
Alias: U2 snRNP complex subunit LEA1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kress T, Krogan N, Guthrie C. A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast. Mol Cell. 2008;32:727-34 pubmed publisher
    ..This result provides strong evidence that an SR protein can promote recruitment of splicing factors to chromatin. ..
  2. Caspary F, Seraphin B. The yeast U2A'/U2B complex is required for pre-spliceosome formation. EMBO J. 1998;17:6348-58 pubmed
    ..revealed a gene potentially coding for a protein with striking similarities to human U2A', henceforth called LEA1 (looks exceptionally like U2A'). We demonstrate that Lea1p is a specific component of the yeast U2 snRNP...
  3. Görnemann J, Kotovic K, Hujer K, Neugebauer K. Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol Cell. 2005;19:53-63 pubmed
    ..Thus, the demonstration of an essential link between CBC and spliceosome assembly in vivo indicates that 5' end capping couples pre-mRNA splicing to transcription. ..
  4. Lacadie S, Rosbash M. Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast. Mol Cell. 2005;19:65-75 pubmed
    ..A 5'ss-U1 snRNA complementation experiment suggests that pairing between U1 and the 5'ss occurs after U1 recruitment and contributes to a specific U1:substrate conformation required for efficient U2 and tri-snRNP recruitment. ..
  5. Gunderson F, Merkhofer E, Johnson T. Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangements. Proc Natl Acad Sci U S A. 2011;108:2004-9 pubmed publisher
    ..H3 residues leads to synthetic lethality when combined with deletion of the genes encoding the U2 snRNP components Lea1 or Msl1...
  6. Gunderson F, Johnson T. Acetylation by the transcriptional coactivator Gcn5 plays a novel role in co-transcriptional spliceosome assembly. PLoS Genet. 2009;5:e1000682 pubmed publisher
    ..of the SAGA complex, has genetic interactions with the genes encoding the heterodimeric U2 snRNP proteins Msl1 and Lea1. These interactions are dependent upon the HAT activity of Gcn5, suggesting a functional relationship between Gcn5 ..
  7. Caspary F, Shevchenko A, Wilm M, Seraphin B. Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly. EMBO J. 1999;18:3463-74 pubmed
    ..The characteristics of Rse1p suggest that its human homologue could be a subunit of the SF3 splicing factor. ..
  8. Schwer B, Kruchten J, Shuman S. Structure-function analysis and genetic interactions of the SmG, SmE, and SmF subunits of the yeast Sm protein ring. RNA. 2016;22:1320-8 pubmed publisher
    ..of SmG, SmE, and SmF (and of SmB and SmD3) were synthetically lethal with null alleles of U2 snRNP subunits Lea1 and Msl1...
  9. Neves L, Douglass S, Spreafico R, Venkataramanan S, Kress T, Johnson T. The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae. Genes Dev. 2017;31:702-717 pubmed publisher
    ..Together, these data demonstrate that H2A.Z is required for efficient pre-mRNA splicing and indicate a role for H2A.Z in coordinating the kinetics of transcription elongation and splicing. ..

More Information

Publications17

  1. Dix I, Russell C, Yehuda S, Kupiec M, Beggs J. The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae. RNA. 1999;5:360-8 pubmed
    ..Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1 p is important in the optimization of splicing. ..
  2. SORENSON M, Jha D, Ucles S, Flood D, Strahl B, Stevens S, et al. Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae. RNA Biol. 2016;13:412-26 pubmed publisher
    ..Additionally, we found that deletion of SET2 reduces the association of the U2 and U5 snRNPs with chromatin. Thus, our study provides the first evidence that H3K36 methylation plays a role in co-transcriptional RNA splicing in yeast. ..
  3. Hausmann S, Zheng S, Costanzo M, Brost R, Garcin D, Boone C, et al. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways. J Biol Chem. 2008;283:31706-18 pubmed publisher
    ..implicated in small nuclear ribonucleoprotein function and spliceosome assembly, including Mud2, Nam8, Brr1, Lea1, Ist3, Isy1, Cwc21, and Bud13...
  4. Kong K, Tang H, Pan K, Huang Z, Lee T, Hinnebusch A, et al. Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing. Nucleic Acids Res. 2014;42:643-60 pubmed publisher
  5. Liang W, Cheng S. A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence. Genes Dev. 2015;29:81-93 pubmed publisher
    ..Mutations impairing U2-branch site base-pairing retard Prp5 release and impede tri-snRNP association. Prp5 mutations that destabilize the Prp5-U2 interaction suppress branch site mutations by allowing progression of the pathway. ..
  6. Hossain M, Claggett J, Nguyen T, Johnson T. The cap binding complex influences H2B ubiquitination by facilitating splicing of the SUS1 pre-mRNA. RNA. 2009;15:1515-27 pubmed publisher
  7. Shieh G, Pan C, Wu J, Sun Y, Wang C, Hsiao W, et al. H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast. BMC Genomics. 2011;12:627 pubmed publisher
    ..In addition, the deletion of genes encoding the U2 snRNP subunits, Lea1 or Msl1, in combination with an htb-K123R mutation, leads to synthetic lethality...
  8. Schwer B, Roth A, Shuman S. Will the circle be unbroken: specific mutations in the yeast Sm protein ring expose a requirement for assembly factor Brr1, a homolog of Gemin2. RNA. 2017;23:420-430 pubmed publisher
    ..i>N66A, R97K, D99A) that were synthetically lethal with null alleles of U2 snRNP subunits Lea1 and/or Msl1...