LCB1

Summary

Gene Symbol: LCB1
Description: serine C-palmitoyltransferase LCB1
Alias: END8, TSC2, serine C-palmitoyltransferase LCB1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Breslow D, Collins S, Bodenmiller B, Aebersold R, Simons K, Shevchenko A, et al. Orm family proteins mediate sphingolipid homeostasis. Nature. 2010;463:1048-53 pubmed publisher
    ..Our work identifies the Orm proteins as critical mediators of sphingolipid homeostasis and raises the possibility that sphingolipid misregulation contributes to the development of childhood asthma. ..
  2. Nagiec M, Baltisberger J, Wells G, Lester R, Dickson R. The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis. Proc Natl Acad Sci U S A. 1994;91:7899-902 pubmed
    ..Here we demonstrate that overproduction in Saccharomyces cerevisiae requires expression of LCB1, a previously isolated yeast gene, and LCB2, the isolation and characterization of which we describe...
  3. Mayor T, Graumann J, Bryan J, MacCoss M, Deshaies R. Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway. Mol Cell Proteomics. 2007;6:1885-95 pubmed
    ..This approach illustrates the feasibility of systems-level quantitative analysis to map enzyme-substrate networks in the UPS. ..
  4. Beeler T, Bacikova D, Gable K, Hopkins L, Johnson C, Slife H, et al. The Saccharomyces cerevisiae TSC10/YBR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2Delta mutant. J Biol Chem. 1998;273:30688-94 pubmed
    ..The recombinant protein catalyzes the NADPH-dependent reduction of 3-ketosphinganine. These data indicate that Tsc10p is necessary and sufficient for catalyzing the NADPH-dependent reduction of 3-ketosphinganine to dihydrosphingosine. ..
  5. Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, et al. A proteomics approach to understanding protein ubiquitination. Nat Biotechnol. 2003;21:921-6 pubmed
    ..The methodology described here provides a general tool for the large-scale analysis and characterization of protein ubiquitination. ..
  6. Sun Y, Miao Y, Yamane Y, Zhang C, Shokat K, Takematsu H, et al. Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Mol Biol Cell. 2012;23:2388-98 pubmed publisher
    ..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner. ..
  7. Han S, Lone M, Schneiter R, Chang A. Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality control. Proc Natl Acad Sci U S A. 2010;107:5851-6 pubmed publisher
    ..Thus, the yeast Orm proteins control membrane biogenesis by coordinating lipid homeostasis with protein quality control. ..
  8. Gable K, Slife H, Bacikova D, Monaghan E, Dunn T. Tsc3p is an 80-amino acid protein associated with serine palmitoyltransferase and required for optimal enzyme activity. J Biol Chem. 2000;275:7597-603 pubmed
    ..The LCB1/TSC2 and LCB2/TSC1 genes encode homologous proteins of the alpha-oxoamine synthase family required for serine ..
  9. Meier K, Deloche O, Kajiwara K, Funato K, Riezman H. Sphingoid base is required for translation initiation during heat stress in Saccharomyces cerevisiae. Mol Biol Cell. 2006;17:1164-75 pubmed
    Sphingolipids are required for many cellular functions including response to heat shock. We analyzed the yeast lcb1-100 mutant, which is conditionally impaired in the first step of sphingolipid biosynthesis and shows a strong decrease in ..

More Information

Publications26

  1. Gable K, Han G, Monaghan E, Bacikova D, Natarajan M, Williams R, et al. Mutations in the yeast LCB1 and LCB2 genes, including those corresponding to the hereditary sensory neuropathy type I mutations, dominantly inactivate serine palmitoyltransferase. J Biol Chem. 2002;277:10194-200 pubmed
    ..We found that the corresponding mutations in the LCB1 gene of Saccharomyces cerevisiae reduce serine palmitoyltransferase activity...
  2. Tani M, Kuge O. Defect of synthesis of very long-chain fatty acids confers resistance to growth inhibition by inositol phosphorylceramide synthase repression in yeast Saccharomyces cerevisiae. J Biochem. 2010;148:565-71 pubmed publisher
    ..However, with repression of LCB1 encoding serine palmitoyltransferase or LIP1 encoding the ceramide synthase subunit, the ELO3 mutation did not ..
  3. Hitchcock A, Auld K, Gygi S, Silver P. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Proc Natl Acad Sci U S A. 2003;100:12735-40 pubmed
    ..Interestingly, we also identified novel membrane-bound transcription factors that may be subject to ubiquitin/proteasome-mediated cleavage and activation at the ER membrane. ..
  4. Nagiec M, Wells G, Lester R, Dickson R. A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase. J Biol Chem. 1993;268:22156-63 pubmed
  5. Watanabe R, Funato K, Venkataraman K, Futerman A, Riezman H. Sphingolipids are required for the stable membrane association of glycosylphosphatidylinositol-anchored proteins in yeast. J Biol Chem. 2002;277:49538-44 pubmed
    ..They could act either by direct involvement as membrane components or as substrates for the remodeling of GPI lipid moieties. ..
  6. Bagnat M, Simons K. Cell surface polarization during yeast mating. Proc Natl Acad Sci U S A. 2002;99:14183-8 pubmed
    ..Our results show that membrane microdomain clustering at the mating projection is involved in the generation and maintenance of polarity during mating. ..
  7. Toume M, Tani M. Change in activity of serine palmitoyltransferase affects sensitivity to syringomycin E in yeast Saccharomyces cerevisiae. FEMS Microbiol Lett. 2014;358:64-71 pubmed publisher
    ..On the contrary, overexpression of Orm2 resulted in high sensitivity to the toxin. Moreover, overexpression of Lcb1 and Lcb2, catalytic subunits of serine palmitoyltransferase, causes resistance to the toxin, whereas partial ..
  8. Toume M, Tani M. Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels. FEBS J. 2016;283:2911-28 pubmed publisher
    ..In contrast, partial repression of LCB1-encoding SPT suppressed abnormal phenotypes caused by the deletion of RVS167, including supersensitivity to high ..
  9. Shimobayashi M, Oppliger W, Moes S, Jeno P, Hall M. TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis. Mol Biol Cell. 2013;24:870-81 pubmed publisher
    ..Thus activation of Orm and complex sphingolipid synthesis upon TORC1 inhibition is a physiological response to starvation. ..
  10. Mitsui K, Hatakeyama K, Matsushita M, Kanazawa H. Saccharomyces cerevisiae Na+/H+ antiporter Nha1p associates with lipid rafts and requires sphingolipid for stable localization to the plasma membrane. J Biochem. 2009;145:709-20 pubmed publisher
    ..In lcb1-100 cells, which are temperature-sensitive for sphingolipid synthesis, newly synthesized Nha1p failed to localize ..
  11. Munn A, Riezman H. Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol. 1994;127:373-86 pubmed
    ..Seven of the Vsl- mutants are defective in endocytosis. Four of these mutant strains (end8-1, end9-1, end10-1, and end11-1) show altered uptake of the endocytosed ligand, alpha-factor, and three (end12-1, ..
  12. Vionnet C, Roubaty C, Ejsing C, Knudsen J, Conzelmann A. Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors. J Biol Chem. 2011;286:6769-79 pubmed publisher
    ..However, lcb1? cells, unable to make sphingoid bases and inositolphosphorylceramides, are viable if they harbor SLC1-1, a gain of ..
  13. Epstein S, Kirkpatrick C, Castillon G, Muñiz M, Riezman I, David F, et al. Activation of the unfolded protein response pathway causes ceramide accumulation in yeast and INS-1E insulinoma cells. J Lipid Res. 2012;53:412-20 pubmed publisher
    ..The LCB1 gene encodes a subunit of the serine palmitoyltransferase, which is responsible for the first step of sphingolipid ..
  14. Cowart L, Okamoto Y, Lu X, Hannun Y. Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae. Biochem J. 2006;393:733-40 pubmed
    ..of sphingolipid production was confirmed by the finding that ISC1 deletion is synthetically lethal with the lcb1-100 mutation...
  15. Pinto W, Wells G, Lester R. Characterization of enzymatic synthesis of sphingolipid long-chain bases in Saccharomyces cerevisiae: mutant strains exhibiting long-chain-base auxotrophy are deficient in serine palmitoyltransferase activity. J Bacteriol. 1992;174:2575-81 pubmed
    ..cerevisiae that require a sphingolipid long-chain base for growth fall into two genetic complementation groups, LCB1 and LCB2...
  16. Ziv I, Matiuhin Y, Kirkpatrick D, Erpapazoglou Z, Leon S, Pantazopoulou M, et al. A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis. Mol Cell Proteomics. 2011;10:M111.009753 pubmed publisher
    ..We conclude that despite the shared use of the ubiquitin molecule, the two branches of the ubiquitin machinery--the ubiquitin-proteasome system and the ubiquitin trafficking system--were unevenly perturbed by expression of K0 ubiquitin. ..
  17. Wang Q, Chang A. Sphingoid base synthesis is required for oligomerization and cell surface stability of the yeast plasma membrane ATPase, Pma1. Proc Natl Acad Sci U S A. 2002;99:12853-8 pubmed
    ..In temperature-sensitive lcb1-100 cells, defective in sphingoid base synthesis, Pma1 fails to oligomerize...