Genomes and Genes
Gene Symbol: KTI12
Alias: TOT4, Kti12p
Species: Saccharomyces cerevisiae S288c
- Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
- Frohloff F, Fichtner L, Jablonowski D, Breunig K, Schaffrath R. Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J. 2001;20:1993-2003 pubmed..In addition, TOT4 and TOT5 (isoallelic with KTI12 and IKI1, respectively) code for components that associate with ELONGATOR: Intriguingly, strains lacking non-..
- Jun S, Cho K, Hwang J, Abdel Fattah W, Hammermeister A, Schaffrath R, et al. Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12. Mol Cells. 2015;38:243-50 pubmed publisher..In a previous study, we showed that the DRL1 gene, which encodes a homolog of the Elongator-associated protein KTI12 of yeast, acts as a positive regulator of adaxial leaf patterning and shoot meristem activity...
- Mehlgarten C, Prochaska H, Hammermeister A, Abdel Fattah W, Wagner M, Krutyhołowa R, et al. Use of a Yeast tRNase Killer Toxin to Diagnose Kti12 Motifs Required for tRNA Modification by Elongator. Toxins (Basel). 2017;9: pubmed publisher..Here, we show that zymocin can be used as a tool to genetically analyse KTI12, a gene previously shown to code for an Elongator partner protein...
- Uthman S, BÃ¤r C, Scheidt V, Liu S, Ten Have S, Giorgini F, et al. The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network. PLoS Genet. 2013;9:e1003334 pubmed publisher..Through identification of Dph6 and Dph7 as components required for the amidation step of the diphthamide pathway, our work paves the way for a detailed mechanistic understanding of diphthamide formation. ..
- Han L, Kon Y, Phizicky E. Functional importance of Î¨38 and Î¨39 in distinct tRNAs, amplified for tRNAGln(UUG) by unexpected temperature sensitivity of the s2U modification in yeast. RNA. 2015;21:188-201 pubmed publisher..These results provide evidence for important roles of both Î¨38 and Î¨39 in specific tRNAs, and establish that modification of the wobble position is subject to change under relatively mild growth conditions. ..
- Abdel Fattah W, Jablonowski D, Di Santo R, ThÃ¼ring K, Scheidt V, Hammermeister A, et al. Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast. PLoS Genet. 2015;11:e1004931 pubmed publisher..and Ser-1209, we provide evidence that phosphorylation plays a positive role in the tRNA modification function of Elongator and may regulate the interaction of Elongator both with its accessory protein Kti12 and with Hrr25 kinase.
- Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher..Collectively, our data suggest that proper TOR signaling requires intact tRNA modifications and that loss of U34 modifications impinges on the TOR-sensitive NCR branch via Gln3 misregulation. ..
- Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher..In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability. ..
- Woodacre A, Lone M, Jablonowski D, Schneiter R, Giorgini F, Schaffrath R. A novel Sit4 phosphatase complex is involved in the response to ceramide stress in yeast. Oxid Med Cell Longev. 2013;2013:129645 pubmed publisher..We also find that the Sit4p target Elongator is not involved in the ceramide response but that cells deficient in Kti12p-an accessory protein with an undefined regulatory role-have similar ceramide phenotypes to sit4? mutants...