Gene Symbol: KTI11
Description: Kti11p
Alias: DPH3, Kti11p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Sun J, Zhang J, Wu F, Xu C, Li S, Zhao W, et al. Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module. Biochemistry. 2005;44:8801-9 pubmed
    b>Kti11p is a small, highly conserved CSL zinc finger-containing protein found in many eukaryotes...
  2. Proudfoot M, Sanders S, Singer A, Zhang R, Brown G, Binkowski A, et al. Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain. J Mol Biol. 2008;375:301-15 pubmed
    ..Likewise, the purified Zn ribbon protein KTI11 from Saccharomyces cerevisiae had a purple color in solution and an Rd-like absorption spectrum, contained both ..
  3. Bär C, Zabel R, Liu S, Stark M, Schaffrath R. A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3. Mol Microbiol. 2008;69:1221-33 pubmed publisher
    The Kluyveromyces lactis killer toxin zymocin insensitive 11 (KTI11) gene from Saccharomyces cerevisiae is allelic with the diphthamide synthesis 3 (DPH3) locus...
  4. Fichtner L, Jablonowski D, Schierhorn A, Kitamoto H, Stark M, Schaffrath R. Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification. Mol Microbiol. 2003;49:1297-307 pubmed
    ..Using TAP tagging, Kti11p contacts Elongator and translational proteins (Rps7Ap, Rps19Ap Eft2p, Yil103wp, Dph2p)...
  5. Liu S, Milne G, Kuremsky J, Fink G, Leppla S. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Mol Cell Biol. 2004;24:9487-97 pubmed
    ..The physiological function of diphthamide and the basis of its ubiquity remain a mystery, but evidence is presented that Dph1 to -3 function in vivo as a protein complex in multiple cellular processes. ..
  6. Fichtner L, Schaffrath R. KTI11 and KTI13, Saccharomyces cerevisiae genes controlling sensitivity to G1 arrest induced by Kluyveromyces lactis zymocin. Mol Microbiol. 2002;44:865-75 pubmed
    ..b>Kti11p is highly conserved from yeast to man, and Kti13p/Ats1p is related to yeast Prp20p and mammalian RCC1, components ..
  7. Greenwood C, Selth L, Dirac Svejstrup A, Svejstrup J. An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator. J Biol Chem. 2009;284:141-9 pubmed publisher
    ..FeS cluster was required for Elongator integrity and for the association of the complex with its accessory factors Kti11 and Kti12...
  8. Uthman S, Bär C, Scheidt V, Liu S, Ten Have S, Giorgini F, et al. The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network. PLoS Genet. 2013;9:e1003334 pubmed publisher
    ..Through identification of Dph6 and Dph7 as components required for the amidation step of the diphthamide pathway, our work paves the way for a detailed mechanistic understanding of diphthamide formation. ..
  9. Yu M, Yang F, Chu W, Wang Y, Zhao H, Gao B, et al. 3D local structure around Zn in Kti11p as a representative Zn-(Cys)4 motif as obtained by MXAN. Biochem Biophys Res Commun. 2008;374:28-32 pubmed publisher
    Zinc is an important component of many proteins that play key roles in transcription, translation, and catalysis. Kti11p, DESR1, both belonging to a protein family characterized by a CSL zinc finger domain, and the co-catalytic zinc-..

More Information


  1. Kolaj Robin O, McEwen A, Cavarelli J, Séraphin B. Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification. FEBS J. 2015;282:819-33 pubmed publisher
    ..b>Kti11 was additionally shown to be implicated in the biosynthesis of diphthamide, a post-translationally modified ..
  2. Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher
    ..Here, we show that Kti13 co-purifies with the Elongator partner protein Kti11 and that the Kti11 interaction, not the Nap1 partnership, requires the C-terminus of Kti13...
  3. Lin Z, Dong M, Zhang Y, Lee E, Lin H. Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification. Nat Chem Biol. 2016;12:995-997 pubmed publisher
    Diphthamide and the tRNA wobble uridine modifications both require diphthamide biosynthesis 3 (Dph3) protein as an electron donor for the iron-sulfur clusters in their biosynthetic enzymes...
  4. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  5. Abdel Fattah W, Scheidt V, Uthman S, Stark M, Schaffrath R. Insights into diphthamide, key diphtheria toxin effector. Toxins (Basel). 2013;5:958-68 pubmed publisher
    ..coli and co-immune precipitation in yeast, that Dph1 and Dph2 interact and that they form a complex with Dph3. Protein-protein interaction mapping shows that Dph1-Dph3 complex formation can be dissected by progressive DPH1 ..
  6. Glatt S, Zabel R, Vonkova I, Kumar A, Netz D, Pierik A, et al. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure. 2015;23:149-60 pubmed publisher
    The small, highly conserved Kti11 alias Dph3 protein encoded by the Kluyveromyces lactis killer toxin insensitive gene KTI11/DPH3 is involved in the diphthamide modification of eukaryotic elongation factor 2 and, together with Kti13, in ..