Gene Symbol: KRE6
Description: beta-glucan synthesis-associated protein KRE6
Alias: CWH48, beta-glucan synthesis-associated protein KRE6
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Pan H, Wang N, Tachikawa H, Nakanishi H, Gao X. β-1,6-glucan synthesis-associated genes are required for proper spore wall formation in Saccharomyces cerevisiae. Yeast. 2017;34:431-446 pubmed publisher
    ..In the present study, mutants defective in β-1,6-glucan synthesis, including kre1∆, kre6∆, kre9∆ and big1∆, were sporulated to analyse the effect of β-1,6-glucan defects on the spore ..
  2. Bowen S, Wheals A. Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6. FEMS Yeast Res. 2004;4:731-5 pubmed
    b>KRE6 (YPR159W) encodes a Golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. A functional Kre6p is necessary for cell wall protein accumulation in response to changing metabolic conditions...
  3. Ketela T, Green R, Bussey H. Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol. 1999;181:3330-40 pubmed
    ..In addition, we have identified an open reading frame, MTL1, which encodes a protein with both structural and functional similarity to Mid2p. ..
  4. Andrews P, Stark M. Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae. J Cell Sci. 2000;113 ( Pt 3):507-20 pubmed
    ..Dephosphorylation by PP1 therefore functions positively to promote cell integrity, bud morphology and polarization of the actin cytoskeleton and glc7-10 cells require higher levels of Pkc1p activity to sustain these functions. ..
  5. Li H, Page N, Bussey H. Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p. Yeast. 2002;19:1097-112 pubmed
    ..EM image analysis and beta-1,6-glucan localization indicated abnormal wall proliferation in the mother cells of these mutants. The pattern of cell wall hypertrophy indicates a failure to restrict cell wall growth to the bud. ..
  6. Kurita T, Noda Y, Takagi T, Osumi M, Yoda K. Kre6 protein essential for yeast cell wall beta-1,6-glucan synthesis accumulates at sites of polarized growth. J Biol Chem. 2011;286:7429-38 pubmed publisher
    Saccharomyces cerevisiae Kre6 is a type II membrane protein with amino acid sequence homology with glycoside hydrolase and is essential for ?-1,6-glucan synthesis as revealed by the mutant phenotype, but its biochemical function is still ..
  7. Takeuchi M, Kimata Y, Hirata A, Oka M, Kohno K. Saccharomyces cerevisiae Rot1p is an ER-localized membrane protein that may function with BiP/Kar2p in protein folding. J Biochem. 2006;139:597-605 pubmed
    ..This implies that the protein folding machinery in which Rot1p is involved chaperones proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies. ..
  8. Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmed
    ..The indispensability of BIG1 and KRE6 for the viability of gas1Delta cells confirmed the important role of beta-1,6-glucan in cells that are defective in ..
  9. Dijkgraaf G, Abe M, Ohya Y, Bussey H. Mutations in Fks1p affect the cell wall content of beta-1,3- and beta-1,6-glucan in Saccharomyces cerevisiae. Yeast. 2002;19:671-90 pubmed
    ..We analysed Fks1p and Fks2p in beta-1,6-glucan deficient mutants and found that they were mislocalized and that the mutants had reduced in vitro glucan synthase activity, possibly contributing to the observed beta-1,6-glucan defects. ..

More Information


  1. Jiang B, Sheraton J, Ram A, Dijkgraaf G, Klis F, Bussey H. CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly. J Bacteriol. 1996;178:1162-71 pubmed
    ..CWH41 also displays strong genetic interactions with KRE1 and KRE6, two genes known to be involved in the beta 1,6-glucan biosynthetic pathway...
  2. Brown J, Bussey H. The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly. Mol Cell Biol. 1993;13:6346-56 pubmed
    ..Double mutants were generated by crossing kre9 delta strains with strains harboring a null mutation in the KRE1, KRE6, or KRE11 gene, and each of these double mutants was found to be inviable in the SEY6210 background...
  3. Roemer T, Delaney S, Bussey H. SKN1 and KRE6 define a pair of functional homologs encoding putative membrane proteins involved in beta-glucan synthesis. Mol Cell Biol. 1993;13:4039-48 pubmed
    b>KRE6 encodes a predicted type II membrane protein which, when disrupted, results in a slowly growing, killer toxin-resistant mutant possessing half the normal level of a structurally wild-type cell wall (1-->6)-beta-glucan (T...
  4. Sasaki T, Toh e A, Kikuchi Y. Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Mol Gen Genet. 2000;262:940-8 pubmed
    ..and six of the genes were identified: tmr1/cyr1, tmnr2/sch9, tmr3/zuo1, tmr4, tmr5/mot1, tmr6/sse1, tmr7 and tmr8/kre6. These results suggested that the tom1 phenotype can be rescued by down-regulating the cAMP/PKA pathway...
  5. Roemer T, Paravicini G, Payton M, Bussey H. Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly. J Cell Biol. 1994;127:567-79 pubmed
    A characterization of the S. cerevisiae KRE6 and SKN1 gene products extends previous genetic studies on their role in (1-->6)-beta-glucan biosynthesis (Roemer, T., and H. Bussey. 1991...
  6. Levinson J, Shahinian S, Sdicu A, Tessier D, Bussey H. Functional, comparative and cell biological analysis of Saccharomyces cerevisiae Kre5p. Yeast. 2002;19:1243-59 pubmed
    ..b>KRE6, encoding a glucanase-like protein, was identified as a multicopy suppressor of a temperature-sensitive kre5 allele,..
  7. Vink E, Vossen J, Ram A, van den Ende H, Brekelmans S, de Nobel H, et al. The protein kinase Kic1 affects 1,6-beta-glucan levels in the cell wall of Saccharomyces cerevisiae. Microbiology. 2002;148:4035-48 pubmed
    ..It is proposed that Kic1 affects cell wall construction in multiple ways and in particular in regulating 1,6-beta-glucan levels in the wall. ..
  8. Imazu H, Sakurai H. Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryot Cell. 2005;4:1050-6 pubmed
    ..Several of the other suppressors were found to encode proteins functioning in cell wall organization. These results suggest that Hsf1 in concert with Pkc1 regulates cell wall remodeling in response to heat shock. ..
  9. Brown J, Kossaczka Z, Jiang B, Bussey H. A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis. Genetics. 1993;133:837-49 pubmed
    ..Genetic interactions among cells harboring mutations at the KRE11, KRE6 and KRE1 loci indicate lethality of kre11 kre6 double mutants and that kre11 is epistatic to kre1, with both gene ..
  10. Takeuchi M, Kimata Y, Kohno K. Saccharomyces cerevisiae Rot1 is an essential molecular chaperone in the endoplasmic reticulum. Mol Biol Cell. 2008;19:3514-25 pubmed publisher
    ..In addition, their dependency on Rot1 appeared different. We therefore propose that Rot1 is a general chaperone with some substrate specificity. ..
  11. Kurita T, Noda Y, Yoda K. Action of multiple endoplasmic reticulum chaperon-like proteins is required for proper folding and polarized localization of Kre6 protein essential in yeast cell wall ?-1,6-glucan synthesis. J Biol Chem. 2012;287:17415-24 pubmed publisher
    Saccharomyces cerevisiae Kre6 is a type II membrane protein essential for cell wall ?-1,6-glucan synthesis...
  12. Breton A, Schaeffer J, Aigle M. The yeast Rvs161 and Rvs167 proteins are involved in secretory vesicles targeting the plasma membrane and in cell integrity. Yeast. 2001;18:1053-68 pubmed
    ..Synthetic defects for growth are also observed with mutation in KRE6, a gene coding for a glucan synthase, required for cell wall construction...
  13. Montijn R, Vink E, Muller W, Verkleij A, van den Ende H, Henrissat B, et al. Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae. J Bacteriol. 1999;181:7414-20 pubmed
    ..structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1...
  14. Popolo L, Gilardelli D, Bonfante P, Vai M. Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae. J Bacteriol. 1997;179:463-9 pubmed
    ..Chitin is essential to compensate for the defects due to the lack of Ggp1p. Moreover, the activities of Ggp1p and Chs3p are essential to the formation of the organized structure of the cell wall in vegetative cells. ..
  15. Nakamata K, Kurita T, Bhuiyan M, Sato K, Noda Y, Yoda K. KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae. J Biol Chem. 2007;282:34315-24 pubmed
    ..Immunoprecipitation from the Triton X-100-solubilized cell lysate revealed that Keg1 binds to Kre6, which has been known to participate in beta-1,6-glucan synthesis...