Gene Symbol: KIN4
Description: putative serine/threonine protein kinase KIN4
Alias: KIN3, KIN31
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Valerio Santiago M, Monje Casas F. Tem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function. J Cell Biol. 2011;192:599-614 pubmed publisher
    ..We also find that removal of Tem1 from the SPBs is critical for the SPOC to impede cell cycle progression. Finally, we demonstrate for the first time that localization of Tem1 to the SPBs is a requirement for mitotic exit. ..
  2. Maekawa H, Priest C, Lechner J, Pereira G, Schiebel E. The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol. 2007;179:423-36 pubmed
    ..Spc72, a transforming acidic coiled coil-like molecule that interacts with the gamma-tubulin complex, recruits Kin4 kinase to both SPBs when cytoplasmic MTs are defective...
  3. Chan L, Amon A. The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4. Genes Dev. 2009;23:1639-49 pubmed publisher
    ..To date, the only component of the checkpoint to be identified is the protein kinase Kin4. Furthermore, how the kinase is regulated by spindle position is not known...
  4. Pereira G, Schiebel E. Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell. 2005;19:209-21 pubmed
    ..Here, we show that the Kin4 kinase is a component of the SPOC and as such is essential to delay cell cycle progression of cells with a ..
  5. Moore J, Chudalayandi P, Heil Chapdelaine R, Cooper J. The spindle position checkpoint is coordinated by the Elm1 kinase. J Cell Biol. 2010;191:493-503 pubmed publisher
    ..Furthermore, we show that Elm1 promotes the activity of the checkpoint kinase Kin4. These findings reveal a novel function for Elm1 in the SPC and suggest how checkpoint activity may be linked to ..
  6. Caydasi A, Kurtulmus B, Orrico M, Hofmann A, Ibrahim B, Pereira G. Elm1 kinase activates the spindle position checkpoint kinase Kin4. J Cell Biol. 2010;190:975-89 pubmed publisher
    ..The cortical kinase Kin4 acts near the top of this network...
  7. Chan L, Amon A. Spindle position is coordinated with cell-cycle progression through establishment of mitotic exit-activating and -inhibitory zones. Mol Cell. 2010;39:444-54 pubmed publisher
    ..Only when an SPB escapes the MEN inhibitor Kin4 in the mother cell and moves into the bud where the MEN activator Lte1 resides can exit from mitosis occur...
  8. Bertazzi D, Kurtulmus B, Pereira G. The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4. J Cell Biol. 2011;193:1033-48 pubmed publisher
    ..Key SPOC components are the kinase Kin4 and the Bub2-Bfa1 GAP complex that inhibit the mitotic exit-promoting GTPase Tem1...
  9. Falk J, Chan L, Amon A. Lte1 promotes mitotic exit by controlling the localization of the spindle position checkpoint kinase Kin4. Proc Natl Acad Sci U S A. 2011;108:12584-90 pubmed publisher
    ..The protein kinase Kin4 is a central component of the spindle position checkpoint...

More Information


  1. Geymonat M, Spanos A, de Bettignies G, Sedgwick S. Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1. J Cell Biol. 2009;187:497-511 pubmed publisher
    ..We observed that lte1 mutants display aberrant localization of the polarity cap, which is the organizer of the actin cytoskeleton. We propose that Lte1's role in cell polarization underlies its contribution to mitotic regulation. ..
  2. Kim J, Jang S, Song K. Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations. Mol Biol Cell. 2008;19:4328-40 pubmed publisher
    ..Instead, decreased interaction of Bfa1 with Kin4 was observed in BFA1 mutant cells with a defective spindle position checkpoint...
  3. D Aquino K, Monje Casas F, Paulson J, Reiser V, Charles G, Lai L, et al. The protein kinase Kin4 inhibits exit from mitosis in response to spindle position defects. Mol Cell. 2005;19:223-34 pubmed
    ..We identified the protein kinase Kin4 as a component of the spindle position checkpoint...
  4. Kim J, Luo G, Bahk Y, Song K. Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit. PLoS Genet. 2012;8:e1002450 pubmed publisher
    ..We suggest that Cdc5 induces the asymmetric distribution of Bfa1 to the bud-directed SPB independently of Bfa1 GAP activity at anaphase and that Bfa1 asymmetry fine-tunes the timing of MEN activation in unperturbed cell cycles. ..
  5. Kambouris N, Burke D, Creutz C. Cloning and genetic analysis of the gene encoding a new protein kinase in Saccharomyces cerevisiae. Yeast. 1993;9:141-50 pubmed
    ..Due to the apparent structural similarity between this kinase and the protein products of the KIN1 and KIN2 genes, we have chosen to name this new gene KIN3.
  6. Merlini L, Fraschini R, Boettcher B, Barral Y, Lucchini G, Piatti S. Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck. PLoS Genet. 2012;8:e1002670 pubmed publisher
    ..SPOC signalling requires the Kin4 protein kinase and the Kin4-regulating Elm1 kinase, which also controls septin dynamics...
  7. Caydasi A, Micoogullari Y, Kurtulmus B, Palani S, Pereira G. The 14-3-3 protein Bmh1 functions in the spindle position checkpoint by breaking Bfa1 asymmetry at yeast centrosomes. Mol Biol Cell. 2014;25:2143-51 pubmed publisher
    ..Upon spindle misalignment, phosphorylation of the SPOC component Bfa1 by Kin4 kinase engages the SPOC by changing the centrosome localization of Bfa1 from asymmetric (one centrosome) to ..
  8. Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S. Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit. PLoS Genet. 2015;11:e1004938 pubmed publisher
    ..Thus, asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis. ..
  9. Falk J, Campbell I, Joyce K, Whalen J, Seshan A, Amon A. LTE1 promotes exit from mitosis by multiple mechanisms. Mol Biol Cell. 2016;27:3991-4001 pubmed
    ..cell compartment, cells arrest in anaphase due to inhibition of the MEN by the mother cell-restricted SPoC kinase Kin4. Here we show that a bud-localized activating signal is necessary for full MEN activation...
  10. Paumi C, Menendez J, Arnoldo A, Engels K, Iyer K, Thaminy S, et al. Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis. Mol Cell. 2007;26:15-25 pubmed
    ..Our study provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins...
  11. Zuzuarregui A, Kupka T, Bhatt B, Dohnal I, Mudrak I, Friedmann C, et al. M-Track: detecting short-lived protein-protein interactions in vivo. Nat Methods. 2012;9:594-6 pubmed publisher
  12. Wei W, Smith N, Wu X, Kim H, Seravalli J, Khalimonchuk O, et al. YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. 2014;21:1475-89 pubmed publisher