Gene Symbol: KEX2
Description: kexin KEX2
Alias: QDS1, SRB1, VMA45, kexin KEX2
Species: Saccharomyces cerevisiae S288c
Products:     KEX2

Top Publications

  1. Fuller R, Brake A, Thorner J. Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. Proc Natl Acad Sci U S A. 1989;86:1434-8 pubmed
    The KEX2-encoded endoprotease was overproduced in yeast several hundred-fold and further purified to achieve a 10,000-fold enrichment in specific activity...
  2. Sipos G, Brickner J, Brace E, Chen L, Rambourg A, Kepes F, et al. Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins. Mol Biol Cell. 2004;15:3196-209 pubmed
  3. Julius D, Brake A, Blair L, Kunisawa R, Thorner J. Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor. Cell. 1984;37:1075-89 pubmed
    S. cerevisiae kex2 mutants are defective for the production of two biologically active secreted peptides: killer toxin and the mating pheromone, alpha-factor. Both molecules are excised from larger precursor polypeptides...
  4. Komano H, Fuller R. Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeast. Proc Natl Acad Sci U S A. 1995;92:10752-6 pubmed
    The MKC7 gene was isolated as a multicopy suppressor of the cold-sensitive growth phenotype of a yeast kex2 mutant, which lacks the protease that cleaves pro-alpha-factor and other secretory proproteins at pairs of basic residues in a ..
  5. Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmed
    ..Unexpectedly, we found that the KEX2 gene is also required for the viability of the gas1Delta mutant...
  6. Deloche O, Yeung B, Payne G, Schekman R. Vps10p transport from the trans-Golgi network to the endosome is mediated by clathrin-coated vesicles. Mol Biol Cell. 2001;12:475-85 pubmed
    ..The Vps10p C-terminal domain appears to play a principal role in retrieval of Vps10p from the prevacuolar compartment rather than in sorting from the TGN...
  7. Redding K, Brickner J, Marschall L, Nichols J, Fuller R. Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol. 1996;16:6208-17 pubmed
    b>Kex2 protease (Kex2p) and Ste13 dipeptidyl aminopeptidase (Ste13p) are required in Saccharomyces cerevisiae for maturation of the alpha-mating factor in a late Golgi compartment, most likely the yeast trans-Golgi network (TGN)...
  8. Brickner J, Fuller R. SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals. J Cell Biol. 1997;139:23-36 pubmed
    Localization of yeast Kex2 protease to the TGN requires a signal (TLS1) in its cytosolic tail (C-tail). Mutation of TLS1 results in rapid transit of Kex2p to the vacuole...
  9. Howard J, Hutton J, Olson J, Payne G. Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis. J Cell Biol. 2002;157:315-26 pubmed
    ..Thus, through a novel NPF-binding domain, Sla1p serves as an endocytic targeting signal adaptor, providing a means to couple cargo with clathrin- and actin-based endocytic machineries. ..

More Information


  1. Yamagishi H, Ohnuki S, Nogami S, Ogata T, Ohya Y. Role of bottom-fermenting brewer's yeast KEX2 in high temperature resistance and poor proliferation at low temperatures. J Gen Appl Microbiol. 2010;56:297-312 pubmed
    ..Similar variants of laboratory yeast were also isolated and found to be incapable of mating. The KEX2 gene was cloned by complementation...
  2. Grbavac A, Čanak I, Stuparevic I, Teparić R, Mrsa V. Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan. Biochim Biophys Acta Mol Cell Res. 2017;1864:507-515 pubmed publisher
    ..It was further shown that non-covalently bound Scw4 (SDS released) underwent the Kex2 proteolytic processing...
  3. Manfredi M, Antunes A, Jesus L, Juliano M, Juliano L, Judice W. Specificity characterization of the ?-mating factor hormone by Kex2 protease. Biochimie. 2016;131:149-158 pubmed publisher
    b>Kex2 is a Ca2+-dependent serine protease from S. cerevisiae...
  4. Davie J, Kane C. Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex. Mol Cell Biol. 2000;20:5960-73 pubmed
    ..These include UBP3, KEX2, STT4, and SWI2/SNF2...
  5. Basco R, Cueva R, Andaluz E, Larriba G. In vivo processing of the precursor of the major exoglucanase by KEX2 endoprotease in the Saccharomyces cerevisiae secretory pathway. Biochim Biophys Acta. 1996;1310:110-8 pubmed
    ..Conversion of A into B was prevented in the double mutant sec7 kex2-1, indicating that Kex2p is central to the in vivo processing...
  6. Bader O, Krauke Y, Hube B. Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, Saccharomyces cerevisiae and Pichia pastoris. BMC Microbiol. 2008;8:116 pubmed publisher
    ..In the yeast Saccharomyces cerevisiae the Kex2 protein is biochemically well investigated, however, with the exception of a few well known proteins such as the ..
  7. Bryant N, Boyd A. Immunoisolation of Kex2p-containing organelles from yeast demonstrates colocalisation of three processing proteinases to a single Golgi compartment. J Cell Sci. 1993;106 ( Pt 3):815-22 pubmed
    ..Our findings provide clear evidence for a single yeast Golgi compartment containing all three late-processing enzymes, which is likely to be the functional equivalent in yeast of the mammalian trans-Golgi network. ..
  8. Oluwatosin Y, Kane P. Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the Kex2 endoprotease in vacuolar acidification. Mol Cell Biol. 1998;18:1534-43 pubmed accumulate the lysosomotropic dye quinacrine in their vacuoles, five new vma complementation groups (vma41 to vma45) were identified...
  9. Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmed
    ..The KEX2 gene encodes a processing protease in the late Golgi compartment and its loss also results in defects in the cell ..
  10. Aguilar P, Heiman M, Walther T, Engel A, Schwudke D, Gushwa N, et al. Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating. Proc Natl Acad Sci U S A. 2010;107:4170-5 pubmed publisher
    ..Thus, a two-atom change in the aliphatic moiety of ergosterol is sufficient to obstruct cell shape remodeling and cell fusion. ..
  11. Cho J, Noda Y, Yoda K. Proteins in the early golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p. FEBS Lett. 2000;469:151-4 pubmed
  12. Heiman M, Engel A, Walter P. The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating. J Cell Biol. 2007;176:209-22 pubmed
    ..In this study, we show that lack of the Golgi-resident protease Kex2 strongly enhances the cell fusion defect of Prm1-deficient mating pairs and causes a mild fusion defect in ..
  13. De M, Abazeed M, Fuller R. Direct binding of the Kex2p cytosolic tail to the VHS domain of yeast Gga2p facilitates TGN to prevacuolar compartment transport and is regulated by phosphorylation. Mol Biol Cell. 2013;24:495-509 pubmed publisher
    ..Thus yeast Gga adaptors facilitate TGN-PVC transport by direct binding of noncanonical phosphoregulated Gga-binding sites in cargo molecules. ..
  14. Rehling P, Darsow T, Katzmann D, Emr S. Formation of AP-3 transport intermediates requires Vps41 function. Nat Cell Biol. 1999;1:346-53 pubmed
    ..Vps41 binds to the AP-3 delta-adaptin subunit, suggesting that they function together in the formation of ALP pathway transport intermediates at the late Golgi. ..
  15. Wächter A, Schwappach B. The yeast CLC chloride channel is proteolytically processed by the furin-like protease Kex2p in the first extracellular loop. FEBS Lett. 2005;579:1149-53 pubmed
    ..Fragments mimicking the N- and C-terminal products of the cleavage reaction are non-functional when expressed alone. However, functional channels can assemble when the two fragments are co-expressed. ..
  16. Martin C, Young R. KEX2 mutations suppress RNA polymerase II mutants and alter the temperature range of yeast cell growth. Mol Cell Biol. 1989;9:2341-9 pubmed
    ..mutation rpb1-1 and produced a cold-sensitive phenotype were all found to be alleles of a single gene, SRB1. An SRB1 partial deletion mutant was further investigated and found to exhibit several pleiotropic phenotypes...
  17. Tomishige N, Noda Y, Adachi H, Yoda K. SKG6, a suppressor gene of synthetic lethality of kex2Delta gas1Delta mutations, encodes a novel membrane protein showing polarized intracellular localization. J Gen Appl Microbiol. 2005;51:323-6 pubmed
  18. Dubé A, Bélanger M, Gagnon Arsenault I, Bourbonnais Y. N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase. BMC Microbiol. 2015;15:50 pubmed publisher