Gene Symbol: KAP114
Description: Kap114p
Alias: Kap114p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Morehouse H, Buratowski R, Silver P, Buratowski S. The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A. 1999;96:12542-7 pubmed
    ..The other high copy suppressor, YGL241W, also known as KAP114, is one of 14 importin/karyopherin proteins in yeast...
  2. Pemberton L, Rosenblum J, Blobel G. Nuclear import of the TATA-binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting. J Cell Biol. 1999;145:1407-17 pubmed
    ..We show here that nuclear import of TBP is mediated by a new karyopherin (Kap) (importin) family member, Kap114p. Kap114p is localized to the cytoplasm and nucleus...
  3. Hahn S, Schlenstedt G. Importin ?-type nuclear transport receptors have distinct binding affinities for Ran-GTP. Biochem Biophys Res Commun. 2011;406:383-8 pubmed publisher
    ..We found that the number of ?-receptors altogether about equals the amounts of yeast Ran, but Ran-GTP is not limiting in the nucleus. The implications of our results for nucleocytoplasmic transport mechanisms are discussed. ..
  4. Kobor M, Venkatasubrahmanyam S, Meneghini M, Gin J, Jennings J, Link A, et al. A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin. PLoS Biol. 2004;2:E131 pubmed
    ..Metazoan orthologs of Swr1p (Drosophila Domino; human SRCAP and p400) may have analogous functions...
  5. Fries T, Betz C, Sohn K, Caesar S, Schlenstedt G, Bailer S. A novel conserved nuclear localization signal is recognized by a group of yeast importins. J Biol Chem. 2007;282:19292-301 pubmed
    ..In vitro, the nuclear localization signal of Asr1p directly interacts with the importins Kap114p, Kap95p, Pse1p, Kap123p, or Kap104p, interactions that are sensitive to the presence of RanGTP...
  6. Peña C, Schütz S, Fischer U, Chang Y, Panse V. Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation. elife. 2016;5: pubmed publisher
    ..Thus, prefabrication of a native-like r-protein subcomplex drives efficient and accurate construction of the eukaryotic ribosome. ..
  7. Baccarini L, Martínez Montañés F, Rossi S, Proft M, Portela P. PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae. Biochim Biophys Acta. 2015;1849:1329-39 pubmed publisher
    ..Moreover, β-karyopherin mutant strains abolished the chromatin association of Tpk1 or Tpk2, suggesting that nuclear localization of PKA catalytic subunits is required for its association to target genes and properly gene expression. ..
  8. Straube K, Blackwell J, Pemberton L. Nap1 and Chz1 have separate Htz1 nuclear import and assembly functions. Traffic. 2010;11:185-97 pubmed publisher
    ..However, Kap114 and Kap123 appeared to play the major role in Htz1 import...
  9. Calvert M, Keck K, Ptak C, Shabanowitz J, Hunt D, Pemberton L. Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol. 2008;28:1313-25 pubmed
    ..In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase. ..

More Information


  1. Caesar S, Greiner M, Schlenstedt G. Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Mol Cell Biol. 2006;26:3170-80 pubmed
    ..parallel import pathways exist for Rpf1, since nuclear import is defective in strains with the importins Kap120, Kap114, and Nmd5 deleted. Both kap120 and rpf1 mutants accumulate large ribosomal subunits in the nucleus...
  2. Mosammaparast N, Ewart C, Pemberton L. A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J. 2002;21:6527-38 pubmed
    ..We demonstrate that Nap1p can bind directly to Kap114p, the primary karyopherin/importin responsible for the nuclear import of H2A and H2B...
  3. Mosammaparast N, Jackson K, Guo Y, Brame C, Shabanowitz J, Hunt D, et al. Nuclear import of histone H2A and H2B is mediated by a network of karyopherins. J Cell Biol. 2001;153:251-62 pubmed
    ..An abundant complex of H2A, H2B, and Kap114p was detected in cytosol...
  4. Hodges J, Leslie J, Mosammaparast N, Guo Y, Shabanowitz J, Hunt D, et al. Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains. Mol Biol Cell. 2005;16:3200-10 pubmed
    ..The yeast karyopherin Kap114p has previously been shown to import histones H2A and H2B, Nap1p, and a component of the preinitiation complex (..
  5. Mosammaparast N, Del Rosario B, Pemberton L. Modulation of histone deposition by the karyopherin kap114. Mol Cell Biol. 2005;25:1764-78 pubmed
    ..However, the coordinate regulation of these processes remains poorly understood. Here we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import of histones H2A and H2B, modulates the ..
  6. Süel K, Chook Y. Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus. J Biol Chem. 2009;284:15416-24 pubmed publisher
    ..More generally, steady-state localization of a nuclear protein is dictated by its nuclear import and export activities as well as its interactions in the nucleus and the cytoplasm. ..
  7. Greiner M, Caesar S, Schlenstedt G. The histones H2A/H2B and H3/H4 are imported into the yeast nucleus by different mechanisms. Eur J Cell Biol. 2004;83:511-20 pubmed
    ..The essential Pse1p and the nonessential importins Kap114p, Kap104p, and Yrb4p/Kap123p specifically bind to histones H2A and H2B...
  8. Miyaji Yamaguchi M, Kato K, Nakano R, Akashi T, Kikuchi A, Nagata K. Involvement of nucleocytoplasmic shuttling of yeast Nap1 in mitotic progression. Mol Cell Biol. 2003;23:6672-84 pubmed
    ..This study also provides a possible explanation for Nap1's involvement in nucleosome assembly and/or remodeling in the nucleus. ..
  9. Rothenbusch U, Sawatzki M, Chang Y, Caesar S, Schlenstedt G. Sumoylation regulates Kap114-mediated nuclear transport. EMBO J. 2012;31:2461-72 pubmed publisher
    The nuclear import receptor Kap114 carries transcription factors and other cargos across nuclear pores into the nucleus...