Gene Symbol: KAP104
Description: Kap104p
Alias: Kap104p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lange A, Mills R, Devine S, Corbett A. A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1. J Biol Chem. 2008;283:12926-34 pubmed publisher
    ..The apparent ortholog of Kapbeta2 in yeast, Kap104, has two known cargos, the mRNA-binding proteins Hrp1 and Nab2, which both contain putative PY-NLS-like sequences...
  2. Fries T, Betz C, Sohn K, Caesar S, Schlenstedt G, Bailer S. A novel conserved nuclear localization signal is recognized by a group of yeast importins. J Biol Chem. 2007;282:19292-301 pubmed
    ..nuclear localization signal of Asr1p directly interacts with the importins Kap114p, Kap95p, Pse1p, Kap123p, or Kap104p, interactions that are sensitive to the presence of RanGTP. In vivo, these importins cooperate in nuclear import...
  3. Siomi M, Fromont M, Rain J, Wan L, Wang F, Legrain P, et al. Functional conservation of the transportin nuclear import pathway in divergent organisms. Mol Cell Biol. 1998;18:4141-8 pubmed
    ..Saccharomyces cerevisiae transportin (yTRN; also known as YBR017c or Kap104p) has been identified and cloned...
  4. Lee D, Aitchison J. Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and Rna. J Biol Chem. 1999;274:29031-7 pubmed
    b>Kap104p is a Saccharomyces cerevisiae nuclear import receptor for two essential mRNA-binding proteins, Nab2p and Nab4p/Hrp1p. We demonstrate direct binding of Kap104p to each of these substrates...
  5. Maurer P, Redd M, Solsbacher J, Bischoff F, Greiner M, Podtelejnikov A, et al. The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p). Mol Biol Cell. 2001;12:539-49 pubmed
    ..This Yrb1p/Xpo1p/Gsp1p complex is then completely dissociated after GTP hydrolysis catalyzed by the cytoplasmic GTPase activating protein Rna1p. ..
  6. Aitchison J, Blobel G, Rout M. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science. 1996;274:624-7 pubmed
    A cytosolic yeast karyopherin, Kap104p, was isolated and shown to function in the nuclear import of a specific class of proteins...
  7. Truant R, Fridell R, Benson R, Bogerd H, Cullen B. Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol. 1998;18:1449-58 pubmed
    ..A yeast homolog of karyopherin beta2, termed Kap104p, has also been described and proposed to play a role in the nuclear import of a yeast hnRNP-like protein termed ..
  8. Dheur S, Nykamp K, Viphakone N, Swanson M, Minvielle Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. J Biol Chem. 2005;280:24532-8 pubmed
    ..Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly(A) tail length during de novo synthesis in yeast. ..
  9. Neville M, Stutz F, Lee L, Davis L, Rosbash M. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export. Curr Biol. 1997;7:767-75 pubmed
    ..Our findings also agree well with current experiments on Crm1p orthologs in Schizosaccharomyces pombe and in vertebrate systems. ..

More Information


  1. van den Bogaart G, Meinema A, Krasnikov V, Veenhoff L, Poolman B. Nuclear transport factor directs localization of protein synthesis during mitosis. Nat Cell Biol. 2009;11:350-6 pubmed publisher
    ..proteins Nab2p and Nab4p/Hrp1p accompany transcripts to their translation site, where the karyopherin Kap104p mediates both their dissociation from the mRNA and their transport back into the nucleus...
  2. Asakawa K, Toh e A. A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae. Genetics. 2002;162:1545-56 pubmed
    A subgroup of the karyopherin beta (also called importin beta) protein that includes budding yeast Kap104 and human transportin/karyopherin beta2 is reported to function as a receptor for the transport of mRNA-binding proteins into the ..
  3. Hahn S, Schlenstedt G. Importin ?-type nuclear transport receptors have distinct binding affinities for Ran-GTP. Biochem Biophys Res Commun. 2011;406:383-8 pubmed publisher
    ..We found that the number of ?-receptors altogether about equals the amounts of yeast Ran, but Ran-GTP is not limiting in the nucleus. The implications of our results for nucleocytoplasmic transport mechanisms are discussed. ..
  4. Süel K, Chook Y. Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus. J Biol Chem. 2009;284:15416-24 pubmed publisher
    ..E., Gu, H., and Chook, Y. M. (2008) PLoS Biol. 6, e137). In this study, we validate Tfg2p as a Kap104p substrate and examine the energetic organization of its PY-NLS...
  5. Leslie D, Zhang W, Timney B, Chait B, Rout M, Wozniak R, et al. Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import. Mol Cell Biol. 2004;24:8487-503 pubmed
    ..b>Kap104p is also known to recognize rg-NLSs, and here we show that it compensates for the loss of Kap121p function...
  6. Schütz S, Fischer U, Altvater M, Nerurkar P, Peña C, Gerber M, et al. A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. elife. 2014;3:e03473 pubmed publisher
    ..We anticipate similar carriers-termed here escortins-to securely connect the nuclear import machinery with pathways that deposit r-proteins onto developing pre-ribosomal particles. ..
  7. Greiner M, Caesar S, Schlenstedt G. The histones H2A/H2B and H3/H4 are imported into the yeast nucleus by different mechanisms. Eur J Cell Biol. 2004;83:511-20 pubmed
    ..The essential Pse1p and the nonessential importins Kap114p, Kap104p, and Yrb4p/Kap123p specifically bind to histones H2A and H2B...
  8. Süel K, Gu H, Chook Y. Modular organization and combinatorial energetics of proline-tyrosine nuclear localization signals. PLoS Biol. 2008;6:e137 pubmed publisher
    ..PY-NLSs) are recognized and transported into the nucleus by human Karyopherin (Kap) beta2/Transportin and yeast Kap104p. Multipartite PY-NLSs are highly diverse in sequence and structure, share a common C-terminal R/H/KX2-5PY motif, ..
  9. Veenhoff L, Meinema A, Poolman B. A karyopherin acts in localized protein synthesis. Cell Cycle. 2010;9:1281-5 pubmed
    ..Recent studies in Saccharomyces cerevisiae suggest that Karyopherin Kap104p plays a role not only in mRNA export but also in bud-localized protein synthesis...
  10. Peña C, Schütz S, Fischer U, Chang Y, Panse V. Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation. elife. 2016;5: pubmed publisher
    ..Thus, prefabrication of a native-like r-protein subcomplex drives efficient and accurate construction of the eukaryotic ribosome. ..