Gene Symbol: JAC1
Description: J-type chaperone JAC1
Alias: J-type chaperone JAC1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Knieszner H, Schilke B, Dutkiewicz R, D Silva P, Cheng S, Ohlson M, et al. Compensation for a defective interaction of the hsp70 ssq1 with the mitochondrial Fe-S cluster scaffold isu. J Biol Chem. 2005;280:28966-72 pubmed
    ..for Isu, the ATPase activity of Ssq1(V472F) was stimulated less well than that of Ssq1 upon addition of Isu and Jac1, the J-protein partner of Ssq1...
  2. Strain J, Lorenz C, Bode J, Garland S, Smolen G, Ta D, et al. Suppressors of superoxide dismutase (SOD1) deficiency in Saccharomyces cerevisiae. Identification of proteins predicted to mediate iron-sulfur cluster assembly. J Biol Chem. 1998;273:31138-44 pubmed
    ..A second sod1Delta suppressor gene, designated JAC1, represents a new member of the 20-kDa J-protein family of co-chaperones...
  3. Kim R, Saxena S, Gordon D, Pain D, Dancis A. J-domain protein, Jac1p, of yeast mitochondria required for iron homeostasis and activity of Fe-S cluster proteins. J Biol Chem. 2001;276:17524-32 pubmed
    ..Here we describe the isolation of mutants of the J-domain protein, Jac1p, using the same screen. Mutant jac1 alleles predicted to encode severely truncated proteins (lacking 70 or 152 amino acids) were associated with ..
  4. Saha P, Kumar S, Srivastava S, Sinha D, Pareek G, D Silva P. The presence of multiple cellular defects associated with a novel G50E iron-sulfur cluster scaffold protein (ISCU) mutation leads to development of mitochondrial myopathy. J Biol Chem. 2014;289:10359-77 pubmed publisher
    ..Thus, our findings provide compelling evidence that the respiration defect due to impaired biogenesis of Fe-S clusters in myopathy patients leads to manifestation of complex clinical symptoms. ..
  5. Mozolewska M, Krupa P, Scheraga H, Liwo A. Molecular modeling of the binding modes of the iron-sulfur protein to the Jac1 co-chaperone from Saccharomyces cerevisiae by all-atom and coarse-grained approaches. Proteins. 2015;83:1414-26 pubmed publisher
    The iron-sulfur protein 1 (Isu1) and the J-type co-chaperone Jac1 from yeast are part of a huge ATP-dependent system, and both interact with Hsp70 chaperones...
  6. Majewska J, Ciesielski S, Schilke B, Kominek J, Blenska A, Delewski W, et al. Binding of the chaperone Jac1 protein and cysteine desulfurase Nfs1 to the iron-sulfur cluster scaffold Isu protein is mutually exclusive. J Biol Chem. 2013;288:29134-42 pubmed publisher
    ..The transfer process requires Jac1, a J-protein Hsp70 cochaperone...
  7. Andrew A, Dutkiewicz R, Knieszner H, Craig E, Marszalek J. Characterization of the interaction between the J-protein Jac1p and the scaffold for Fe-S cluster biogenesis, Isu1p. J Biol Chem. 2006;281:14580-7 pubmed
    ..In vivo, such JAC1 mutations had no obvious phenotypic effect...
  8. Mokranjac D, Sichting M, Popov Celeketic D, Mapa K, Gevorkyan Airapetov L, Zohary K, et al. Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell. 2009;20:1400-7 pubmed publisher
    ..This function of Tim50 depends on its association with TIM23. We conclude that the efficient transfer of precursors between TOM and TIM23 complexes requires the concerted action of Tim50 with Tim23. ..
  9. Ciesielski S, Schilke B, Osipiuk J, Bigelow L, Mulligan R, Majewska J, et al. Interaction of J-protein co-chaperone Jac1 with Fe-S scaffold Isu is indispensable in vivo and conserved in evolution. J Mol Biol. 2012;417:1-12 pubmed publisher
    The ubiquitous mitochondrial J-protein Jac1, called HscB in Escherichia coli, and its partner Hsp70 play a critical role in the transfer of Fe-S clusters from the scaffold protein Isu to recipient proteins...

More Information


  1. Jensen L, Sanchez R, Srinivasan C, Valentine J, Culotta V. Mutations in Saccharomyces cerevisiae iron-sulfur cluster assembly genes and oxidative stress relevant to Cu,Zn superoxide dismutase. J Biol Chem. 2004;279:29938-43 pubmed
    ..By comparison, the sod1Delta lysine auxotrophy appears to be reversed in the seo mutants by increased expression of genes in the lysine biosynthetic pathway, perhaps through sensing of mitochondrial damage by the retrograde response. ..
  2. Dutkiewicz R, Schilke B, Cheng S, Knieszner H, Craig E, Marszalek J. Sequence-specific interaction between mitochondrial Fe-S scaffold protein Isu and Hsp70 Ssq1 is essential for their in vivo function. J Biol Chem. 2004;279:29167-74 pubmed
    ..of Fe-S clusters in the yeast mitochondrial matrix, is a substrate protein for the Hsp70 Ssq1 and the J-protein Jac1 in vitro...
  3. Dutkiewicz R, Marszalek J, Schilke B, Craig E, Lill R, Muhlenhoff U. The Hsp70 chaperone Ssq1p is dispensable for iron-sulfur cluster formation on the scaffold protein Isu1p. J Biol Chem. 2006;281:7801-8 pubmed
    ..Hence, consistent with previous in vivo data, these chaperones likely function in steps subsequent to the de novo synthesis of the Fe/S cluster on Isu1p. ..
  4. Andrew A, Song J, Schilke B, Craig E. Posttranslational regulation of the scaffold for Fe-S cluster biogenesis, Isu. Mol Biol Cell. 2008;19:5259-66 pubmed publisher
    ..Our results are consistent with the existence of a mechanism to increase the stability of Isu, and thus its level, that is dependent on the presence of the cysteine desulfurase Nfs1. ..
  5. Ciesielski S, Schilke B, Marszalek J, Craig E. Protection of scaffold protein Isu from degradation by the Lon protease Pim1 as a component of Fe-S cluster biogenesis regulation. Mol Biol Cell. 2016;27:1060-8 pubmed publisher
    ..and that it is protected from degradation by Nfs1, the sulfur donor for Fe-S cluster assembly, as well as by Jac1, the J-protein Hsp70 cochaperone that functions in cluster transfer from Isu...