Gene Symbol: ISW2
Description: DNA translocase
Alias: DNA translocase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bachman N, Gelbart M, Tsukiyama T, Boeke J. TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs. Genes Dev. 2005;19:955-64 pubmed
    ..ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection...
  2. Kent N, Karabetsou N, Politis P, Mellor J. In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes Dev. 2001;15:619-26 pubmed
    ..We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth. ..
  3. Gkikopoulos T, Schofield P, Singh V, Pinskaya M, Mellor J, Smolle M, et al. A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. Science. 2011;333:1758-60 pubmed publisher
    ..These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome. ..
  4. Dang W, Bartholomew B. Domain architecture of the catalytic subunit in the ISW2-nucleosome complex. Mol Cell Biol. 2007;27:8306-17 pubmed
    ..topological view of one of these complexes has been revealed, by mapping the interactions of the catalytic subunit Isw2 with nucleosomal and extranucleosomal DNA in the complex with all four subunits of ISW2 bound to nucleosomes...
  5. Tsukiyama T, Palmer J, Landel C, Shiloach J, Wu C. Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev. 1999;13:686-97 pubmed
    We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae, ISW1 and ISW2, which are highly related to Drosophila ISWI, encoding the putative ATPase subunit of three ATP-dependent chromatin remodeling ..
  6. Whitehouse I, Rando O, Delrow J, Tsukiyama T. Chromatin remodelling at promoters suppresses antisense transcription. Nature. 2007;450:1031-5 pubmed
    ..Saccharomyces cerevisiae genome, we have investigated the role of the ATP-dependent chromatin remodelling complex Isw2 in positioning nucleosomes...
  7. Ferreira H, Flaus A, Owen Hughes T. Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms. J Mol Biol. 2007;374:563-79 pubmed
    ..strategy to generate specifically modified chromatin templates and used these to study the interaction of the Chd1, Isw2 and RSC remodelling complexes with differentially acetylated nucleosomes...
  8. Gelbart M, Bachman N, Delrow J, Boeke J, Tsukiyama T. Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant. Genes Dev. 2005;19:942-54 pubmed
    b>Isw2 ATP-dependent chromatin-remodeling activity is targeted to early meiotic and MATa-specific gene promoters in Saccharomyces cerevisiae. Unexpectedly, preferential cross-linking of wild-type Isw2p was not detected at these loci...
  9. Goldmark J, Fazzio T, Estep P, Church G, Tsukiyama T. The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p. Cell. 2000;103:423-33 pubmed
    ..We have found that S. cerevisiae Isw2 complex represses transcription of early meiotic genes during mitotic growth in a parallel pathway to Rpd3-Sin3 ..

More Information


  1. Dechassa M, Hota S, Sen P, Chatterjee N, Prasad P, Bartholomew B. Disparity in the DNA translocase domains of SWI/SNF and ISW2. Nucleic Acids Res. 2012;40:4412-21 pubmed publisher
    An ATP-dependent DNA translocase domain consisting of seven conserved motifs is a general feature of all ATP-dependent chromatin remodelers...
  2. Gelbart M, Rechsteiner T, Richmond T, Tsukiyama T. Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates. Mol Cell Biol. 2001;21:2098-106 pubmed
    ..This system enabled us to analyze the interaction of Isw2 ATP-dependent chromatin remodeling complex with nucleosomal arrays...
  3. Alén C, Kent N, Jones H, O Sullivan J, Aranda A, Proudfoot N. A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Mol Cell. 2002;10:1441-52 pubmed
    ..We suggest that chromatin remodeling of termination regions is a necessary prelude to efficient Pol II termination. ..
  4. McConnell A, Gelbart M, Tsukiyama T. Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo. Mol Cell Biol. 2004;24:2605-13 pubmed
    We report the identification of two new subunits of the Isw2 chromatin-remodeling complex in Saccharomyces cerevisiae...
  5. Gangaraju V, Prasad P, Srour A, Kagalwala M, Bartholomew B. Conformational changes associated with template commitment in ATP-dependent chromatin remodeling by ISW2. Mol Cell. 2009;35:58-69 pubmed publisher
    Distinct stages in ATP-dependent chromatin remodeling are found as ISW2, an ISWI-type complex, forms a stable and processive complex with nucleosomes upon hydrolysis of ATP...
  6. Zofall M, Persinger J, Bartholomew B. Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2. Mol Cell Biol. 2004;24:10047-57 pubmed
    A minimal amount of extranucleosomal DNA was required for nucleosome mobilization by ISW2 as shown by using a photochemical histone mapping approach to analyze nucleosome movement on a set of nucleosomes with varied lengths of ..
  7. Pinskaya M, Nair A, Clynes D, Morillon A, Mellor J. Nucleosome remodeling and transcriptional repression are distinct functions of Isw1 in Saccharomyces cerevisiae. Mol Cell Biol. 2009;29:2419-30 pubmed publisher
    ..Thus, chromatin remodeling and transcriptional repression are distinct activities of Isw1. ..
  8. Iida T, Araki H. Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:217-27 pubmed
    ..We also report a novel and putative chromatin-remodeling complex, ISW2/yCHRAC, that contains Isw2, Itc1, Dpb3-like subunit (Dls1), and Dpb4...
  9. Dang W, Kagalwala M, Bartholomew B. Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA. Mol Cell Biol. 2006;26:7388-96 pubmed
    The stable contact of ISW2 with nucleosomal DNA approximately 20 bp from the dyad was shown by DNA footprinting and photoaffinity labeling using recombinant histone octamers to require the histone H4 N-terminal tail...
  10. Zhang Z, Reese J. Ssn6-Tup1 requires the ISW2 complex to position nucleosomes in Saccharomyces cerevisiae. EMBO J. 2004;23:2246-57 pubmed
    ..Here, we demonstrate that both ISW2 and TUP1 are required to position nucleosomes across the entire coding sequence of the DNA damage-inducible gene ..
  11. Vincent J, Kwong T, Tsukiyama T. ATP-dependent chromatin remodeling shapes the DNA replication landscape. Nat Struct Mol Biol. 2008;15:477-84 pubmed publisher
    ..Here we show that two highly conserved ATP-dependent chromatin-remodeling complexes in Saccharomyces cerevisiae, Isw2 and Ino80, function in parallel to promote replication fork progression...
  12. Fazzio T, Gelbart M, Tsukiyama T. Two distinct mechanisms of chromatin interaction by the Isw2 chromatin remodeling complex in vivo. Mol Cell Biol. 2005;25:9165-74 pubmed
    We have previously shown that Saccharomyces cerevisiae Isw2 complex slides nucleosomes to remodel chromatin in vivo...
  13. Kagalwala M, Glaus B, Dang W, Zofall M, Bartholomew B. Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling. EMBO J. 2004;23:2092-104 pubmed
    Linker DNA was found to be critical for the specific docking of ISW2 with nucleosomes as shown by mapping the physical contacts of ISW2 with nucleosomes at base-pair resolution...
  14. Krajewski W, Reese J. SET domains of histone methyltransferases recognize ISWI-remodeled nucleosomal species. Mol Cell Biol. 2010;30:552-64 pubmed publisher
    ..Our study reveals novel insights into the mechanism of how SET domains recognize different chromatin states and specify histone methylation at active loci. ..
  15. Dang W, Sutphin G, Dorsey J, Otte G, Cao K, Perry R, et al. Inactivation of yeast Isw2 chromatin remodeling enzyme mimics longevity effect of calorie restriction via induction of genotoxic stress response. Cell Metab. 2014;19:952-66 pubmed publisher
    ..We explored functions of chromatin remodelers during cellular aging. Deletion of ISW2, or mutations inactivating the Isw2 enzyme complex, extends yeast replicative lifespan...
  16. Sugiyama M, Nikawa J. The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology. J Bacteriol. 2001;183:4985-93 pubmed
    ..The gene products of ITC1 and ISW2 are known to constitute a chromatin-remodeling complex (T. Tsukiyama, J. Palmer, C. C. Landel, J. Shiloach, and C...
  17. Berger I, Fitzgerald D, Richmond T. Baculovirus expression system for heterologous multiprotein complexes. Nat Biotechnol. 2004;22:1583-7 pubmed
    ..Gene insertion reactions are carried out in Escherichia coli either sequentially or concurrently in a rapid, one-step procedure. Our system is useful for both recombinant multiprotein production and multigene transfer applications. ..
  18. Fazzio T, Kooperberg C, Goldmark J, Neal C, Basom R, Delrow J, et al. Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression. Mol Cell Biol. 2001;21:6450-60 pubmed
    The yeast Isw2 chromatin remodeling complex functions in parallel with the Sin3-Rpd3 histone deacetylase complex to repress early meiotic genes upon recruitment by Ume6p...
  19. Ruiz C, Escribano V, Morgado E, Molina M, Mazón M. Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex. Microbiology. 2003;149:341-51 pubmed
    ..It is suggested that the Isw2p-Itc1p complex contributes to maintain the repressive chromatin structure described for the asg operator present in the promoters of a-specific genes. ..
  20. Deindl S, Hwang W, Hota S, Blosser T, Prasad P, Bartholomew B, et al. ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps. Cell. 2013;152:442-52 pubmed publisher
    ..Our results suggest a remodeling mechanism with well-defined coordination at different nucleosomal sites featuring DNA translocation toward the exit side in 1 bp steps preceding multi-bp steps of DNA movement on the entry side. ..
  21. Gao Q, Madian A, Liu X, Adamec J, Regnier F. Coupling protein complex analysis to peptide based proteomics. J Chromatogr A. 2010;1217:7661-8 pubmed publisher
  22. Ryan D, Sundaramoorthy R, Martin D, Singh V, Owen Hughes T. The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains. EMBO J. 2011;30:2596-609 pubmed publisher
    ..Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome. ..
  23. Trachtulcová P, Frýdlová I, Janatova I, Hasek J. The absence of the Isw2p-Itc1p chromatin-remodelling complex induces mating type-specific and Flo11p-independent invasive growth of Saccharomyces cerevisiae. Yeast. 2004;21:389-401 pubmed
    ..We report here that the deletion of the ISW2 gene in the originally non-invasive BY strain induces mating type-specific invasive growth strongly affected by ..
  24. Ocampo J, Chereji R, Eriksson P, Clark D. The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo. Nucleic Acids Res. 2016;44:4625-35 pubmed publisher
    ..In vitro, the three known yeast nucleosome spacing enzymes (CHD1, ISW1 and ISW2) form arrays with different spacing...
  25. Fischer C, Yamada K, Fitzgerald D. Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2. Biochemistry. 2009;48:2960-8 pubmed publisher
    The chromatin remodeling complex Isw2 from Saccharomyces cerevisiae (yIsw2) mobilizes nucleosomes through an ATP-dependent reaction that is coupled to the translocation of the helicase domain of the enzyme along intranucleosomal DNA...
  26. Zentner G, Tsukiyama T, Henikoff S. ISWI and CHD chromatin remodelers bind promoters but act in gene bodies. PLoS Genet. 2013;9:e1003317 pubmed publisher
    ..discrepancy between in vitro and in vivo observations, we have mapped the genomic distributions of the yeast Isw1, Isw2, and Chd1 remodelers at base-pair resolution on native chromatin...
  27. Parnell T, Schlichter A, WILSON B, Cairns B. The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism. elife. 2015;4:e06073 pubmed publisher
    ..Taken together, we provide the first genetic and genomic evidence for RSC-ISW1a antagonism and reveal different mechanisms at two different promoter architectures. ..
  28. Shetty A, Lopes J. Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex. Eukaryot Cell. 2010;9:1845-55 pubmed publisher
    ..Furthermore, Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter...
  29. Au T, Rodriguez J, Vincent J, Tsukiyama T. ATP-dependent chromatin remodeling factors tune S phase checkpoint activity. Mol Cell Biol. 2011;31:4454-63 pubmed publisher
    ..Here, we show that two highly conserved Saccharomyces cerevisiae ATP-dependent chromatin remodeling factors, Isw2 and Ino80, function to attenuate and deactivate S phase checkpoint activity...
  30. Mueller J, Li C, Bryk M. Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2007;361:1017-21 pubmed
    ..Here, we report the findings of an investigation into the role of ISW2 in transcriptional silencing at the rDNA...
  31. Krajewski W, Vassiliev O. Remodeling of nucleosome-dimer particles with yIsw2 promotes their association with ALL-1 SET domain in vitro. Biochemistry. 2012;51:4354-63 pubmed publisher
    ..Here we investigated whether nucleosome remodeling with the yeast Isw2 affects nucleosome affinity to the SET domain of ALL-1 HKMT...
  32. Yadon A, Singh B, Hampsey M, Tsukiyama T. DNA looping facilitates targeting of a chromatin remodeling enzyme. Mol Cell. 2013;50:93-103 pubmed publisher
    ..Here, we present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-..
  33. Lafon A, Petty E, Pillus L. Functional antagonism between Sas3 and Gcn5 acetyltransferases and ISWI chromatin remodelers. PLoS Genet. 2012;8:e1002994 pubmed publisher
    ..This genetic interaction depends on the ATPase activities of Isw1 and Isw2, suggesting that it involves chromatin remodeling activities driven by the enzymes...
  34. Krajewski W. Mobilization of hyperacetylated mononucleosomes by purified yeast ISW2 in vitro. Arch Biochem Biophys. 2016;591:1-6 pubmed publisher
    ..We evaluated if purified yeast ISW2 can act on mononucleosomes in which all four core histones are highly acetylated...
  35. Sherriff J, Kent N, Mellor J. The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2. Mol Cell Biol. 2007;27:2848-60 pubmed
    ..The Isw2 chromatin-remodeling ATPase cooperates with Fkh2 to remodel the chromatin and repress CLB2 expression throughout ..
  36. Krajewski W. Isw1a does not have strict limitations on the length of extranucleosomal DNAs for mobilization of nucleosomes assembled with HeLa cell histones. J Biomol Struct Dyn. 2014;32:523-31 pubmed publisher
    The Saccharomyces cerevisiae Isw1a and Isw2 ATP-dependent chromatin-remodeling complexes have important roles in vivo in the regulation of nucleosome positioning and modulation of gene activity...
  37. Hota S, Bhardwaj S, Deindl S, Lin Y, Zhuang X, Bartholomew B. Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains. Nat Struct Mol Biol. 2013;20:222-9 pubmed publisher
    ..b>ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain called SLIDE (SANT-like ISWI ..
  38. Tomar R, Psathas J, Zhang H, Zhang Z, Reese J. A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression. Mol Cell Biol. 2009;29:3255-65 pubmed publisher
    ..The gene encoding a large subunit of ribonucleotide reductase, RNR3, is regulated by ISW2 and SWI/SNF, complexes that repress and activate transcription, respectively...
  39. Tackett A, Dilworth D, Davey M, O DONNELL M, Aitchison J, Rout M, et al. Proteomic and genomic characterization of chromatin complexes at a boundary. J Cell Biol. 2005;169:35-47 pubmed
    ..The complexes consist of at least 15 chromatin-associated proteins, including DNA pol epsilon, the Isw2-Itc1 and Top2 chromatin remodeling proteins, the Sas3-Spt16 chromatin modifying complex, and Yta7, a bromodomain-..
  40. Krajewski W. Comparison of the Isw1a, Isw1b, and Isw2 nucleosome disrupting activities. Biochemistry. 2013;52:6940-9 pubmed publisher
    The three Saccharomyces cerevisiae ISWI chromatin remodeling complexes, Isw1a, Isw1b, and Isw2, are implicated in the regularization of arrayed nucleosomes and regulation of gene activity...
  41. Frýdlová I, Basler M, Vasicová P, Malcová I, Hasek J. Special type of pheromone-induced invasive growth in Saccharomyces cerevisiae. Curr Genet. 2007;52:87-95 pubmed
    ..We report here on invasion displayed by MATalpha cells of Saccharomyces cerevisiae lacking Isw2p, a subunit of the ISW2 chromatin remodelling complex...
  42. Evstiukhina T, Alekseeva E, Fedorov D, Peshekhonov V, Korolev V. [The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae]. Genetika. 2017;53:173-80 pubmed
    ..In this paper, we show that single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells...