Gene Symbol: ISW1
Description: chromatin-remodeling ATPase ISW1
Alias: SGN2, chromatin-remodeling ATPase ISW1
Species: Saccharomyces cerevisiae S288c
Products:     ISW1

Top Publications

  1. Kent N, Karabetsou N, Politis P, Mellor J. In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. Genes Dev. 2001;15:619-26 pubmed
    ..We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth. ..
  2. Tsukiyama T, Palmer J, Landel C, Shiloach J, Wu C. Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev. 1999;13:686-97 pubmed
    We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae, ISW1 and ISW2, which are highly related to Drosophila ISWI, encoding the putative ATPase subunit of three ATP-dependent chromatin remodeling ..
  3. Stockdale C, Flaus A, Ferreira H, Owen Hughes T. Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes. J Biol Chem. 2006;281:16279-88 pubmed
    ..These observations suggest that the accessibility of linker DNA together with the positioning properties of the underlying DNA play important roles in determining the outcome of remodeling by these enzymes. ..
  4. Morillon A, Karabetsou N, Nair A, Mellor J. Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription. Mol Cell. 2005;18:723-34 pubmed
    ..The Isw1 ATPase delays the release of initiated RNA polymerase II (RNAPII) into elongation to facilitate chromatin ..
  5. Alén C, Kent N, Jones H, O Sullivan J, Aranda A, Proudfoot N. A role for chromatin remodeling in transcriptional termination by RNA polymerase II. Mol Cell. 2002;10:1441-52 pubmed
    ..We suggest that chromatin remodeling of termination regions is a necessary prelude to efficient Pol II termination. ..
  6. Quan T, Hartzog G. Histone H3K4 and K36 methylation, Chd1 and Rpd3S oppose the functions of Saccharomyces cerevisiae Spt4-Spt5 in transcription. Genetics. 2010;184:321-34 pubmed publisher
    ..We further observed enhancement of cryptic initiation in chd1 isw1 mutants and increased histone acetylation in a chd1 mutant...
  7. Morillon A, Karabetsou N, O Sullivan J, Kent N, Proudfoot N, Mellor J. Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II. Cell. 2003;115:425-35 pubmed
    ..Overall these studies establish the central role played by Isw1p in the coordination of transcription. ..
  8. Biswas D, Dutta Biswas R, Stillman D. Chd1 and yFACT act in opposition in regulating transcription. Mol Cell Biol. 2007;27:6279-87 pubmed
    ..Although human Chd1 has been shown to bind to H3-K4-Me, we discuss evidence arguing that yeast Chd1 binds to neither H3-K4-Me nor H3-K36-Me. ..
  9. Gangaraju V, Bartholomew B. Dependency of ISW1a chromatin remodeling on extranucleosomal DNA. Mol Cell Biol. 2007;27:3217-25 pubmed
    ..ISW1b, the other isoform of ISW1, does not have the same dependency for extranucleosomal DNA as ISW1a and, likewise, is not able to space ..

More Information


  1. Pinskaya M, Nair A, Clynes D, Morillon A, Mellor J. Nucleosome remodeling and transcriptional repression are distinct functions of Isw1 in Saccharomyces cerevisiae. Mol Cell Biol. 2009;29:2419-30 pubmed publisher
    ..we investigate functions in vivo associated with two SANT motifs, SANT and SLIDE, in the Saccharomyces cerevisiae Isw1 chromatin-remodeling ATPase...
  2. Vary J, Gangaraju V, Qin J, Landel C, Kooperberg C, Bartholomew B, et al. Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol. 2003;23:80-91 pubmed
    ..In vivo, the two Isw1 complexes have overlapping functions in transcriptional regulation of some genes yet distinct functions at others...
  3. Xella B, Goding C, Agricola E, Di Mauro E, Caserta M. The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. Mol Microbiol. 2006;59:1531-41 pubmed
    ..Here we show that the absence of the Isw1 and Chd1 ATP-dependent chromatin remodelling activities delays the maximal expression of ADH2 without impairing the ..
  4. Gelbart M, Rechsteiner T, Richmond T, Tsukiyama T. Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates. Mol Cell Biol. 2001;21:2098-106 pubmed
    ..These results demonstrate the utility of our in vitro system in studying interactions between chromatin-associated proteins and nucleosomal arrays. ..
  5. Gkikopoulos T, Schofield P, Singh V, Pinskaya M, Mellor J, Smolle M, et al. A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. Science. 2011;333:1758-60 pubmed publisher
    ..Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization...
  6. Zentner G, Tsukiyama T, Henikoff S. ISWI and CHD chromatin remodelers bind promoters but act in gene bodies. PLoS Genet. 2013;9:e1003317 pubmed publisher
    ..this discrepancy between in vitro and in vivo observations, we have mapped the genomic distributions of the yeast Isw1, Isw2, and Chd1 remodelers at base-pair resolution on native chromatin...
  7. Lindstrom K, Vary J, Parthun M, Delrow J, Tsukiyama T. Isw1 functions in parallel with the NuA4 and Swr1 complexes in stress-induced gene repression. Mol Cell Biol. 2006;26:6117-29 pubmed
    ..The biochemical activities of the yeast Isw1 ATP-dependent chromatin-remodeling enzyme have been well characterized in vitro, but little is known about how ..
  8. Krajewski W. Comparison of the Isw1a, Isw1b, and Isw2 nucleosome disrupting activities. Biochemistry. 2013;52:6940-9 pubmed publisher
    ..The nuclease digestion data suggest that Isw1a can also promote ATP-dependent distortion of nucleosome structure, although less efficiently than the Isw1b complex. ..
  9. Parnell T, Schlichter A, WILSON B, Cairns B. The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism. elife. 2015;4:e06073 pubmed publisher
    ..RSC and ISW1a largely co-localize, and genomic nucleosome studies using rsc isw1 mutant combinations revealed opposing functions: promoters classified with a nucleosome-deficient region (NDR) gain ..
  10. Maltby V, Martin B, Schulze J, Johnson I, Hentrich T, Sharma A, et al. Histone H3 lysine 36 methylation targets the Isw1b remodeling complex to chromatin. Mol Cell Biol. 2012;32:3479-85 pubmed publisher
    ..While H3K36 methylation has been shown to regulate the binding of numerous factors, this is the first time that it has been shown to facilitate targeting of a chromatin-remodeling complex. ..
  11. Deindl S, Hwang W, Hota S, Blosser T, Prasad P, Bartholomew B, et al. ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps. Cell. 2013;152:442-52 pubmed publisher
    ..Our results suggest a remodeling mechanism with well-defined coordination at different nucleosomal sites featuring DNA translocation toward the exit side in 1 bp steps preceding multi-bp steps of DNA movement on the entry side. ..
  12. Lafon A, Petty E, Pillus L. Functional antagonism between Sas3 and Gcn5 acetyltransferases and ISWI chromatin remodelers. PLoS Genet. 2012;8:e1002994 pubmed publisher
    ..This genetic interaction depends on the ATPase activities of Isw1 and Isw2, suggesting that it involves chromatin remodeling activities driven by the enzymes...
  13. Vasicová P, Stradalova V, Halada P, Hasek J, Malcová I. Nuclear import of chromatin remodeler Isw1 is mediated by atypical bipartite cNLS and classical import pathway. Traffic. 2013;14:176-93 pubmed publisher
    The protein Isw1 of Saccharomyces cerevisiae is an imitation-switch chromatin-remodeling factor...
  14. Mueller J, Bryk M. Isw1 acts independently of the Isw1a and Isw1b complexes in regulating transcriptional silencing at the ribosomal DNA locus in Saccharomyces cerevisiae. J Mol Biol. 2007;371:1-10 pubmed
    ..Previous work has shown that the ATP-dependent chromatin remodeling protein Isw1 is required for silencing of a marker gene inserted at the HMR locus, but not at telomeres...
  15. Sherriff J, Kent N, Mellor J. The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2. Mol Cell Biol. 2007;27:2848-60 pubmed
    ..In addition, the related factors Isw1 and Fkh1 configure the chromatin at the early coding region and negatively regulate CLB2 expression but only during ..
  16. Frýdlová I, Malcová I, Vasicová P, Hasek J. Deregulation of DSE1 gene expression results in aberrant budding within the birth scar and cell wall integrity pathway activation in Saccharomyces cerevisiae. Eukaryot Cell. 2009;8:586-94 pubmed publisher
    ..Although the exact cellular role of this daughter cell-specific protein has to be elucidated, our data point to the involvement of Dse1 in bud site selection in haploid cells. ..
  17. Jones H, Kawauchi J, Braglia P, Alen C, Kent N, Proudfoot N. RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat Struct Mol Biol. 2007;14:123-30 pubmed
    ..We suggest that Pol I transcription, like that of Pol II, may be modulated by specific chromatin structures. ..
  18. Chen Y, Chou C, Gartenberg M. Determinants of Sir2-Mediated, Silent Chromatin Cohesion. Mol Cell Biol. 2016;36:2039-50 pubmed publisher
    ..Esc8 was previously shown to associate with Isw1, the enzymatic core of ISW1 chromatin remodelers, to form a variant of the ISW1a chromatin remodeling complex...
  19. Babour A, Shen Q, Dos Santos J, Murray S, Gay A, Challal D, et al. The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis. Cell. 2016;167:1201-1214.e15 pubmed publisher
    ..Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-..
  20. Moreau J, Lee M, Mahachi N, Vary J, Mellor J, Tsukiyama T, et al. Regulated displacement of TBP from the PHO8 promoter in vivo requires Cbf1 and the Isw1 chromatin remodeling complex. Mol Cell. 2003;11:1609-20 pubmed
    ..We show here that on glucose depletion, the S. cerevisiae Isw1 chromatin remodeling complex is required for the displacement of TBP from the PHO8 promoter...
  21. Ludwigsen J, Pfennig S, Singh A, Schindler C, Harrer N, Forne I, et al. Concerted regulation of ISWI by an autoinhibitory domain and the H4 N-terminal tail. elife. 2017;6: pubmed publisher
    ..This configuration is reminiscent of Chd1 even though Chd1 contains an unrelated NTR. Our results shed light on the intricate structural and functional regulation of ISWI by the NTR and uncover surprising parallels with Chd1. ..
  22. Erkina T, Zou Y, Freeling S, Vorobyev V, Erkine A. Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes. Nucleic Acids Res. 2010;38:1441-9 pubmed publisher
    ..chromatin remodeling complexes negligibly affects reporter promoters, combinatorial inactivation of SNF2 and ISW1 has a synergistic effect by diminishing histone loss during heat induction and eliminating Pol II recruitment...
  23. Ryan D, Sundaramoorthy R, Martin D, Singh V, Owen Hughes T. The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains. EMBO J. 2011;30:2596-609 pubmed publisher
    ..Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome. ..
  24. Yamada K, Frouws T, Angst B, Fitzgerald D, DeLuca C, Schimmele K, et al. Structure and mechanism of the chromatin remodelling factor ISW1a. Nature. 2011;472:448-53 pubmed publisher
    ..Results from a remodelling assay corroborate the dinucleosome model. We show how a chromatin remodelling factor could set the spacing between two adjacent nucleosomes acting as a 'protein ruler'. ..
  25. Wohlschlegel J, Johnson E, Reed S, Yates J. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:45662-8 pubmed
    ..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes. ..
  26. Koster M, Yildirim A, Weil P, Holstege F, Timmers H. Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein. Nucleic Acids Res. 2014;42:4220-9 pubmed publisher
    ..Conditional depletion of Mot1p or NC2 in absence of the ISW1 remodeler, but not in the absence of other chromatin remodelers, activated the cryptic FLO8 promoter...
  27. Ocampo J, Chereji R, Eriksson P, Clark D. The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo. Nucleic Acids Res. 2016;44:4625-35 pubmed publisher
    ..In vitro, the three known yeast nucleosome spacing enzymes (CHD1, ISW1 and ISW2) form arrays with different spacing...
  28. Biswas D, Dutta Biswas R, Mitra D, Shibata Y, Strahl B, Formosa T, et al. Opposing roles for Set2 and yFACT in regulating TBP binding at promoters. EMBO J. 2006;25:4479-89 pubmed
    ..These synthetic defects are suppressed by set2, demonstrating that yFACT and Set2 oppose one another during transcriptional initiation at a step involving DNA binding by TBP and TFIIA. ..
  29. Evstiukhina T, Alekseeva E, Fedorov D, Peshekhonov V, Korolev V. [The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae]. Genetika. 2017;53:173-80 pubmed
    ..In this paper, we show that single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells...
  30. Krajewski W. Isw1a does not have strict limitations on the length of extranucleosomal DNAs for mobilization of nucleosomes assembled with HeLa cell histones. J Biomol Struct Dyn. 2014;32:523-31 pubmed publisher
    ..30?bp) extranucleosomal DNAs protruding from both sides, which contrasts to the previously reported inability of Isw1 to mobilize similar nucleosomes assembled with recombinant yeast histones...
  31. Erkina T, ERKINE A. ASF1 and the SWI/SNF complex interact functionally during nucleosome displacement, while FACT is required for nucleosome reassembly at yeast heat shock gene promoters during sustained stress. Cell Stress Chaperones. 2015;20:355-69 pubmed publisher
    ..A similar but less pronounced effect was observed for ISW1/ASF1 inactivation but not for ASF1/STH1 (RSC complex) combinatorial inactivation...
  32. Yu Q, Zhang X, Bi X. Roles of chromatin remodeling factors in the formation and maintenance of heterochromatin structure. J Biol Chem. 2011;286:14659-69 pubmed publisher
    ..Therefore, chromatin remodeling proteins may rearrange nucleosomes during the formation of heterochromatin or serve to stabilize/maintain heterochromatin structure. ..
  33. Yadav T, Whitehouse I. Replication-Coupled Nucleosome Assembly and Positioning by ATP-Dependent Chromatin-Remodeling Enzymes. Cell Rep. 2016;15:715-723 pubmed publisher
    ..Importantly, we find that ATP-dependent chromatin-remodeling enzymes, Isw1 and Chd1, collaborate with histone chaperones to remodel nucleosomes as they are loaded behind a replication fork...
  34. Denison C, Rudner A, Gerber S, Bakalarski C, Moazed D, Gygi S. A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics. 2005;4:246-54 pubmed
    ..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes. ..
  35. Krajewski W. Yeast Isw1a and Isw1b exhibit similar nucleosome mobilization capacities for mononucleosomes, but differently mobilize dinucleosome templates. Arch Biochem Biophys. 2014;546:72-80 pubmed publisher
    ..The data suggest that nucleosome mobilizing activity of Isw1a, Isw1b and Isw2 complexes could be significantly influenced by neighboring nucleosomes. ..