Gene Symbol: IST3
Description: U2 snRNP complex subunit IST3
Alias: SNU17, U2 snRNP complex subunit IST3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zhou Y, Chen C, Johansson M. The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression. Nucleic Acids Res. 2013;41:5669-78 pubmed publisher
    ..Our results demonstrate that TAN1 pre-mRNA processing has an unprecedented requirement for RES factors and that the complex controls the formation of ac(4)C in tRNA. ..
  2. Wang Q, Rymond B. Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus. Mol Cell Biol. 2003;23:7339-49 pubmed
    ..Together these data establish Rds3p as an essential component of the U2 snRNP SF3b complex and suggest a new link between the nuclear processes of pre-mRNA splicing and RNA export. ..
  3. Gottschalk A, Bartels C, Neubauer G, Luhrmann R, Fabrizio P. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol. 2001;21:3037-46 pubmed
    ..Although the SNU17 gene is not essential, its knockout leads to a slow-growth phenotype and to a pre-mRNA splicing defect in vivo...
  4. Dziembowski A, Ventura A, Rutz B, Caspary F, Faux C, Halgand F, et al. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004;23:4847-56 pubmed
    ..The corresponding complex was named pre-mRNA REtention and Splicing (RES). The presence of RES subunit homologues in numerous eukaryotes suggests that its function is evolutionarily conserved. ..
  5. Wang Q, He J, Lynn B, Rymond B. Interactions of the yeast SF3b splicing factor. Mol Cell Biol. 2005;25:10745-54 pubmed
    ..These data establish the concordance of yeast and mammalian SF3b complexes, implicate accessory splicing factors in U2 snRNP function, and support SF3b contribution from early pre-mRNP recognition to late steps in splicing. ..
  6. Schmidlin T, Kaeberlein M, Kudlow B, MacKay V, Lockshon D, Kennedy B. Single-gene deletions that restore mating competence to diploid yeast. FEMS Yeast Res. 2008;8:276-86 pubmed
    ..However, an ist3Delta/ist3Delta strain mates only with MATa haploids. It is shown that IST3, previously linked to splicing, is required for efficient processing of the MATa1 message, particularly the first ..
  7. Hausmann S, Zheng S, Costanzo M, Brost R, Garcin D, Boone C, et al. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways. J Biol Chem. 2008;283:31706-18 pubmed publisher
    ..implicated in small nuclear ribonucleoprotein function and spliceosome assembly, including Mud2, Nam8, Brr1, Lea1, Ist3, Isy1, Cwc21, and Bud13...
  8. Trowitzsch S, Weber G, Luhrmann R, Wahl M. An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex. J Biol Chem. 2008;283:32317-27 pubmed publisher
  9. Brooks M, Dziembowski A, Quevillon Cheruel S, Henriot V, Faux C, van Tilbeurgh H, et al. Structure of the yeast Pml1 splicing factor and its integration into the RES complex. Nucleic Acids Res. 2009;37:129-43 pubmed publisher
    ..This complex was shown to contain three subunits, namely Snu17, Bud13 and Pml1, but its mode of action remains ill-defined...

More Information


  1. Qiu Z, Chico L, Chang J, Shuman S, Schwer B. Genetic interactions of hypomorphic mutations in the m7G cap-binding pocket of yeast nuclear cap binding complex: an essential role for Cbc2 in meiosis via splicing of MER3 pre-mRNA. RNA. 2012;18:1996-2011 pubmed publisher
    ..sickness with null mutations of proteins involved in early steps of spliceosome assembly (Nam8, Mud1, Swt21, Mud2, Ist3, and Brr1) and with otherwise benign mutations of Msl5, the essential branchpoint binding protein...
  2. Wysoczański P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl M, et al. Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nat Struct Mol Biol. 2014;21:911-8 pubmed publisher
    ..The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome. ..
  3. SORENSON M, Jha D, Ucles S, Flood D, Strahl B, Stevens S, et al. Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae. RNA Biol. 2016;13:412-26 pubmed publisher
    ..Additionally, we found that deletion of SET2 reduces the association of the U2 and U5 snRNPs with chromatin. Thus, our study provides the first evidence that H3K36 methylation plays a role in co-transcriptional RNA splicing in yeast. ..
  4. Tripsianes K, Friberg A, Barrandon C, Brooks M, van Tilbeurgh H, Seraphin B, et al. A novel protein-protein interaction in the RES (REtention and Splicing) complex. J Biol Chem. 2014;289:28640-50 pubmed publisher
    ..Our data highlight structural diversity in RRM-protein interactions, analogous to the one seen for nucleic acid interactions. ..
  5. Wysoczański P, Zweckstetter M. Retention and splicing complex (RES) - the importance of cooperativity. RNA Biol. 2016;13:128-33 pubmed publisher
    ..Our work highlights the role of cooperativity in the spliceosome and poses new questions about the structure and assembly of the spliceosome. ..
  6. Neves L, Douglass S, Spreafico R, Venkataramanan S, Kress T, Johnson T. The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae. Genes Dev. 2017;31:702-717 pubmed publisher
    ..Together, these data demonstrate that H2A.Z is required for efficient pre-mRNA splicing and indicate a role for H2A.Z in coordinating the kinetics of transcription elongation and splicing. ..