Gene Symbol: ISA2
Species: Saccharomyces cerevisiae S288c
Pelzer W, Muhlenhoff U, Diekert K, Siegmund K, Kispal G, Lill R. Mitochondrial Isa2p plays a crucial role in the maturation of cellular iron-sulfur proteins. FEBS Lett. 2000;476:134-9 pubmed
..Here, we report on a new component of this machinery, the protein Isa2p of the yeast Saccharomyces cerevisiae...
Rodriguez Manzaneque M, Tamarit J, Belli G, Ros J, Herrero E. Grx5 is a mitochondrial glutaredoxin required for the activity of iron/sulfur enzymes. Mol Biol Cell. 2002;13:1109-21 pubmed
..Phenotypic defects associated with the absence of Grx5 are suppressed by overexpression of SSQ1 and ISA2, two genes involved in the synthesis and assembly of iron/sulfur clusters into proteins...
Venne A, Vögtle F, Meisinger C, Sickmann A, Zahedi R. Novel highly sensitive, specific, and straightforward strategy for comprehensive N-terminal proteomics reveals unknown substrates of the mitochondrial peptidase Icp55. J Proteome Res. 2013;12:3823-30 pubmed publisher
..Interestingly, a novel substrate, Isa2, is likely processed by Icp55 in two consecutive steps and thus might represent the first example of a triple ..
Jensen L, Culotta V. Role of Saccharomyces cerevisiae ISA1 and ISA2 in iron homeostasis. Mol Cell Biol. 2000;20:3918-27 pubmed
The budding yeast Saccharomyces cerevisiae contains two homologues of bacterial IscA proteins, designated Isa1p and Isa2p. Bacterial IscA is a product of the isc (iron-sulfur cluster) operon and has been suggested to participate in Fe-S ..
Gelling C, Dawes I, Richhardt N, Lill R, Muhlenhoff U. Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymes. Mol Cell Biol. 2008;28:1851-61 pubmed
..Iba57p physically interacts with the mitochondrial ISC assembly components Isa1p and Isa2p. Since all three proteins are conserved in eukaryotes and bacteria, the specificity of the Iba57/Isa complex may ..
Muhlenhoff U, Richter N, Pines O, Pierik A, Lill R. Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins. J Biol Chem. 2011;286:41205-16 pubmed publisher
Most eukaryotes contain iron-sulfur cluster (ISC) assembly proteins related to Saccharomyces cerevisiae Isa1 and Isa2. We show here that Isa1 but not Isa2 can be functionally replaced by the bacterial relatives IscA, SufA, and ErpA...
Gaspard G, McMaster C. The mitochondrial quality control protein Yme1 is necessary to prevent defective mitophagy in a yeast model of Barth syndrome. J Biol Chem. 2015;290:9284-98 pubmed publisher
..We identify an important role for the mitochondrial protease Yme1 in the ability of cells that lack tafazzin function to maintain mitochondrial structural integrity and mitochondrial quality control and to undergo mitophagy. ..
Melber A, Na U, Vashisht A, Weiler B, Lill R, Wohlschlegel J, et al
. Role of Nfu1 and Bol3 in iron-sulfur cluster transfer to mitochondrial clients. elife. 2016;5: pubmed publisher
..Given these observations, we propose that Nfu1, assisted by Bol3, functions to facilitate Fe-S transfer from the biosynthetic apparatus to the client proteins preventing oxidative damage to [4Fe-4S] clusters. ..
Muhlenhoff U, Gerl M, Flauger B, Pirner H, Balser S, Richhardt N, et al
. The ISC [corrected] proteins Isa1 and Isa2 are required for the function but not for the de novo synthesis of the Fe/S clusters of biotin synthase in Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:495-504 pubmed
..To identify new components of biotin metabolism, we performed a genetic screen and found that Isa2, a mitochondrial protein involved in the formation of Fe/S proteins, is necessary for the conversion of ..