Gene Symbol: IRR1
Description: Irr1p
Alias: SCC3, Irr1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lin W, Wang M, Jin H, Yu H. Cohesin plays a dual role in gene regulation and sister-chromatid cohesion during meiosis in Saccharomyces cerevisiae. Genetics. 2011;187:1041-51 pubmed publisher
    ..Here we report that the cohesin subunits Scc3 and Smc1 are both required for the production of the meiosis-specific subunit Rec8 in the budding yeast ..
  2. Rowland B, Roig M, Nishino T, Kurze A, Uluocak P, Mishra A, et al. Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity. Mol Cell. 2009;33:763-74 pubmed publisher
    Cohesin's Smc1, Smc3, and Scc1 subunits form a tripartite ring that entraps sister DNAs. Scc3, Pds5, and Rad61 (Wapl) are regulatory subunits that control this process...
  3. Ivanov D, Schleiffer A, Eisenhaber F, Mechtler K, Haering C, Nasmyth K. Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion. Curr Biol. 2002;12:323-8 pubmed
    ..In Saccharomyces cerevisiae, a complex consisting of Scc1, Smc1, Smc3, and Scc3 proteins, called "cohesin," mediates the connection between sister chromatids...
  4. Roig M, Löwe J, Chan K, Beckouët F, Metson J, Nasmyth K. Structure and function of cohesin's Scc3/SA regulatory subunit. FEBS Lett. 2014;588:3692-702 pubmed publisher
    ..Cohesin's association with chromatin involves subunits recruited by Scc1: Wapl, Pds5, and Scc3/SA, in addition to Scc2/4 loading complex...
  5. Mc Intyre J, Muller E, Weitzer S, Snydsman B, Davis T, Uhlmann F. In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae. EMBO J. 2007;26:3783-93 pubmed
    ..Little is known about two additional subunits, Scc3 and Pds5, and about possible conformational changes of the complex during the cell cycle...
  6. Toth A, Ciosk R, Uhlmann F, Galova M, Schleiffer A, Nasmyth K. Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev. 1999;13:320-33 pubmed
    ..The sixth protein, Eco1p(Ctf7p), is not a Cohesin subunit. It is necessary for the establishment of cohesion during DNA replication but not for its maintenance during G2 and M phases. ..
  7. Arumugam P, Gruber S, Tanaka K, Haering C, Mechtler K, Nasmyth K. ATP hydrolysis is required for cohesin's association with chromosomes. Curr Biol. 2003;13:1941-53 pubmed
    ..Cohesin contains four core subunits: Smc1, Smc3, Scc1, and Scc3. Biochemical studies suggest that Smc1 and Smc3 each form 50 nm-long antiparallel coiled coils (arms) and bind to ..
  8. Kulemzina I, Schumacher M, Verma V, Reiter J, Metzler J, Failla A, et al. Cohesin rings devoid of Scc3 and Pds5 maintain their stable association with the DNA. PLoS Genet. 2012;8:e1002856 pubmed publisher
    ..The roles of three additional proteins that associate with the ring, Scc3, Pds5 and Wpl1, are not well understood...
  9. Huang J, Hsu J, Laurent B. The RSC nucleosome-remodeling complex is required for Cohesin's association with chromosome arms. Mol Cell. 2004;13:739-50 pubmed
    ..Our data suggest a role for RSC in facilitating the loading of cohesin specifically onto chromosome arms, thereby ensuring sister chromatid cohesion and proper chromosome segregation. ..

More Information


  1. Białkowska A, Kurlandzka A. Additional copies of the NOG2 and IST2 genes suppress the deficiency of cohesin Irr1p/Scc3p in Saccharomyces cerevisiae. Acta Biochim Pol. 2002;49:421-5 pubmed
    ..Here we describe two dosage suppressors (IST2, NOG2) overcoming the inability to form colonies of an Irr1p-deficient strain...
  2. Cena A, Skoneczny M, Chełstowska A, Kowalec P, Natorff R, Kurlandzka A. Cohesin Irr1/Scc3 is likely to influence transcription in Saccharomyces cerevisiae via interaction with Mediator complex. Acta Biochim Pol. 2013;60:233-8 pubmed
    ..The participation of SA2p (mammalian ortholog of yeast Irr1p, associated with the core of the complex) in the regulation of transcription is already described...
  3. Cena A, Orlowski J, Machula K, Fronk J, Kurlandzka A. Substitution F659G in the Irr1p/Scc3p cohesin influences the cell wall of Saccharomyces cerevisiae. Cell Struct Funct. 2007;32:1-7 pubmed
    ..We investigated the Irr1p/Scc3p cohesin subunit, an under-studied protein...
  4. Beckouët F, Srinivasan M, Roig M, Chan K, Scheinost J, Batty P, et al. Releasing Activity Disengages Cohesin's Smc3/Scc1 Interface in a Process Blocked by Acetylation. Mol Cell. 2016;61:563-574 pubmed publisher
    ..We show that mutations capable of bypassing Eco1 in Smc1, Smc3, Scc1, Wapl, Pds5, and Scc3 subunits reduce dissociation of N-terminal cleavage fragments of Scc1 (NScc1) from Smc3...
  5. Cena A, Kozłowska E, Płochocka D, Grynberg M, Ishikawa T, Fronk J, et al. The F658G substitution in Saccharomyces cerevisiae cohesin Irr1/Scc3 is semi-dominant in the diploid and disturbs mitosis, meiosis and the cell cycle. Eur J Cell Biol. 2008;87:831-44 pubmed publisher
    ..complex of Saccharomyces cerevisiae includes chromosomal ATPases Smc1p and Smc3p, the kleisin Mcd1p/Scc1p, and Irr1p/Scc3p, the least studied component...
  6. Katis V, Lipp J, Imre R, Bogdanova A, Okaz E, Habermann B, et al. Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Dev Cell. 2010;18:397-409 pubmed publisher
    ..Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8. ..
  7. Kurlandzka A, Rytka J, Rozalska B, Wysocka M. Saccharomyces cerevisiae IRR1 protein is indirectly involved in colony formation. Yeast. 1999;15:23-33 pubmed
    ..This indicates that Irr1p is not directly involved in the cell-solid support adhesion, but may be an element of a communication pathway ..
  8. Kowalec P, Fronk J, Kurlandzka A. The Irr1/Scc3 protein implicated in chromosome segregation in Saccharomyces cerevisiae has a dual nuclear-cytoplasmic localization. Cell Div. 2017;12:1 pubmed publisher
    ..The essential, evolutionarily conserved regulatory protein Irr1/Scc3, is responsible for the complex loading onto DNA and for its removal...
  9. Lin W, Jin H, Liu X, Hampton K, Yu H. Scc2 regulates gene expression by recruiting cohesin to the chromosome as a transcriptional activator during yeast meiosis. Mol Biol Cell. 2011;22:1985-96 pubmed publisher
    ..Finally, we provide evidence that chromosomal binding of cohesin is sufficient for target-gene activation during meiosis. Our data support a noncanonical role for cohesin as a transcriptional activator during cell differentiation. ..
  10. Li Z, Vizeacoumar F, Bahr S, Li J, Warringer J, Vizeacoumar F, et al. Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Nat Biotechnol. 2011;29:361-7 pubmed publisher
    ..This mutant collection should facilitate a wide range of systematic studies aimed at understanding the functions of essential genes. ..
  11. Orgil O, Matityahu A, Eng T, Guacci V, Koshland D, Onn I. A conserved domain in the scc3 subunit of cohesin mediates the interaction with both mcd1 and the cohesin loader complex. PLoS Genet. 2015;11:e1005036 pubmed publisher
    ..Cohesin is also important for chromosome condensation, DNA repair, and gene expression. Cohesin is comprised of Scc3, Mcd1, Smc1, and Smc3...
  12. Measday V, Hailey D, Pot I, Givan S, Hyland K, Cagney G, et al. Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 2002;16:101-13 pubmed
    ..In contrast, Ctf3p and Ctf19p fail to bind properly to the centromere in a cse4-1 mutant strain. We conclude that the requirements for CENP-A loading onto centromere DNA differ in fission versus budding yeast. ..
  13. Parnas O, Zipin Roitman A, Mazor Y, Liefshitz B, Ben Aroya S, Kupiec M. The ELG1 clamp loader plays a role in sister chromatid cohesion. PLoS ONE. 2009;4:e5497 pubmed publisher
    ..Our results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring. ..
  14. Fernius J, Nerusheva O, Galander S, Alves F, Rappsilber J, Marston A. Cohesin-dependent association of scc2/4 with the centromere initiates pericentromeric cohesion establishment. Curr Biol. 2013;23:599-606 pubmed publisher
    ..Overall, our findings provide a paradigm for the spatial and temporal control of cohesin loading. ..
  15. Heidinger Pauli J, Onn I, Koshland D. Genetic evidence that the acetylation of the Smc3p subunit of cohesin modulates its ATP-bound state to promote cohesion establishment in Saccharomyces cerevisiae. Genetics. 2010;185:1249-56 pubmed publisher
    ..Finally, we provide evidence that Smc3p acetylation, which has an essential role in cohesion establishment, modulates the Smc3p ATP-bound state. ..
  16. Bialkowska A, Kurlandzka A. Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast. 2002;19:1323-33 pubmed
    ..Using a two-hybrid screen we identified a novel nuclear protein, Lin1, interacting with Irr1p/Scc3p, a component of the cohesin complex...