IRE1

Summary

Gene Symbol: IRE1
Description: bifunctional endoribonuclease/protein kinase IRE1
Alias: ERN1, bifunctional endoribonuclease/protein kinase IRE1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Guo J, Polymenis M. Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae. EMBO Rep. 2006;7:1124-7 pubmed
    ..How the UPR is downregulated is not well understood. Inositol requirement 1 (Ire1) is an endoplasmic reticulum transmembrane UPR sensor in Saccharomyces cerevisiae...
  2. Patil C, Li H, Walter P. Gcn4p and novel upstream activating sequences regulate targets of the unfolded protein response. PLoS Biol. 2004;2:E246 pubmed
    ..The discovery of a role for Gcn4p in the yeast UPR reveals an additional level of complexity and demonstrates a surprising conservation of the signaling circuit between yeast and metazoan cells. ..
  3. Leber J, Bernales S, Walter P. IRE1-independent gain control of the unfolded protein response. PLoS Biol. 2004;2:E235 pubmed
    ..We show that by adding an additional signaling element to the basic UPR circuit, a simple switch is transformed into a complex response. ..
  4. Oikawa D, Kimata Y, Kohno K. Self-association and BiP dissociation are not sufficient for activation of the ER stress sensor Ire1. J Cell Sci. 2007;120:1681-8 pubmed
    b>Ire1 is a type I transmembrane protein located on the endoplasmic reticulum (ER). Upon ER stress, Ire1 releases the ER chaperone BiP and self-associates...
  5. Kimata Y, Ishiwata Kimata Y, Ito T, Hirata A, Suzuki T, Oikawa D, et al. Two regulatory steps of ER-stress sensor Ire1 involving its cluster formation and interaction with unfolded proteins. J Cell Biol. 2007;179:75-86 pubmed
    Chaperone protein BiP binds to Ire1 and dissociates in response to endoplasmic reticulum (ER) stress. However, it remains unclear how the signal transducer Ire1 senses ER stress and is subsequently activated...
  6. Pincus D, Chevalier M, ARAGON T, van Anken E, Vidal S, El Samad H, et al. BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response. PLoS Biol. 2010;8:e1000415 pubmed publisher
    ..The mechanism by which the ER stress sensor Ire1 is activated by unfolded proteins and the role that the ER chaperone protein BiP plays in Ire1 regulation have ..
  7. Cox J, Walter P. A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response. Cell. 1996;87:391-404 pubmed
    ..We propose that the complex regulation of Hac1p expression serves to provide multiple levels at which the UPR can be controlled. ..
  8. ARAGON T, van Anken E, Pincus D, Serafimova I, Korennykh A, Rubio C, et al. Messenger RNA targeting to endoplasmic reticulum stress signalling sites. Nature. 2009;457:736-40 pubmed publisher
    ..ER stress is sensed by Ire1, a transmembrane kinase/endoribonuclease, which initiates the non-conventional splicing of the messenger RNA ..
  9. Friedlander R, Jarosch E, Urban J, Volkwein C, Sommer T. A regulatory link between ER-associated protein degradation and the unfolded-protein response. Nat Cell Biol. 2000;2:379-84 pubmed
    ..However, when stress is applied, the UPR is required to increase ERAD activity. We thus demonstrate, for the first time, a regulatory loop between ERAD and the UPR, which is essential for normal growth of yeast cells. ..

More Information

Publications73

  1. Korennykh A, Egea P, Korostelev A, Finer Moore J, Zhang C, Shokat K, et al. The unfolded protein response signals through high-order assembly of Ire1. Nature. 2009;457:687-93 pubmed publisher
    ..folding of proteins in the endoplasmic reticulum activates the bifunctional transmembrane kinase/endoribonuclease Ire1. Ire1 excises an intron from HAC1 messenger RNA in yeasts and Xbp1 messenger RNA in metozoans encoding homologous ..
  2. Goffin L, Vodala S, Fraser C, Ryan J, Timms M, Meusburger S, et al. The unfolded protein response transducer Ire1p contains a nuclear localization sequence recognized by multiple beta importins. Mol Biol Cell. 2006;17:5309-23 pubmed
    ..The NLS-dependent nuclear localization of Ire1p would thus seem to be central to its role in UPR signaling. ..
  3. Lee K, Dey M, Neculai D, Cao C, Dever T, Sicheri F. Structure of the dual enzyme Ire1 reveals the basis for catalysis and regulation in nonconventional RNA splicing. Cell. 2008;132:89-100 pubmed publisher
    b>Ire1 is an ancient transmembrane sensor of ER stress with dual protein kinase and ribonuclease activities. In response to ER stress, Ire1 catalyzes the splicing of target mRNAs in a spliceosome-independent manner...
  4. Todd Corlett A, Jones E, Seghers C, Gething M. Lobe IB of the ATPase domain of Kar2p/BiP interacts with Ire1p to negatively regulate the unfolded protein response in Saccharomyces cerevisiae. J Mol Biol. 2007;367:770-87 pubmed
    ..Binding of an unfolded polypeptide to the substrate-binding domain of Kar2p could alter the positioning of glutamine 88 and other residues on lobe IB involved in binding Ire1p, releasing Ire1p for activation of UPR signaling. ..
  5. Oikawa D, Kimata Y, Takeuchi M, Kohno K. An essential dimer-forming subregion of the endoplasmic reticulum stress sensor Ire1. Biochem J. 2005;391:135-42 pubmed
    The luminal domain of the type I transmembrane protein Ire1 senses endoplasmic reticulum stress by an undefined mechanism to up-regulate the signalling pathway for the unfolded protein response...
  6. Sugiyama M, Nikawa J. The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology. J Bacteriol. 2001;183:4985-93 pubmed
    In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain...
  7. Pineau L, Colas J, Dupont S, Beney L, Fleurat Lessard P, Berjeaud J, et al. Lipid-induced ER stress: synergistic effects of sterols and saturated fatty acids. Traffic. 2009;10:673-90 pubmed publisher
    ..The present data are discussed in the context of human diseases that involve lipid deregulation. ..
  8. Torres Quiroz F, García Marqués S, Coria R, Randez Gil F, Prieto J. The activity of yeast Hog1 MAPK is required during endoplasmic reticulum stress induced by tunicamycin exposure. J Biol Chem. 2010;285:20088-96 pubmed publisher
    ..Hence, our results describe new aspects of the yeast response to ER stress and identify additional functions of glycerol and the Hog1p MAPK to provide stress resistance. ..
  9. Mori K, Ma W, Gething M, Sambrook J. A transmembrane protein with a cdc2+/CDC28-related kinase activity is required for signaling from the ER to the nucleus. Cell. 1993;74:743-56 pubmed
    ..Several yeast mutants defective in this pathway map to the ERN1 gene, which protects cells from lethal consequences of stress by signaling for increased expression of BiP and ..
  10. Papa F, Zhang C, Shokat K, Walter P. Bypassing a kinase activity with an ATP-competitive drug. Science. 2003;302:1533-7 pubmed
    ..proteins in the endoplasmic reticulum cause trans-autophosphorylation of the bifunctional transmembrane kinase Ire1, which induces its endoribonuclease activity...
  11. Gardner B, Walter P. Unfolded proteins are Ire1-activating ligands that directly induce the unfolded protein response. Science. 2011;333:1891-4 pubmed publisher
    ..Under conditions of ER stress, the transmembrane protein Ire1 oligomerizes to activate its cytoplasmic kinase and ribonuclease domains...
  12. Wiseman R, Zhang Y, Lee K, Harding H, Haynes C, Price J, et al. Flavonol activation defines an unanticipated ligand-binding site in the kinase-RNase domain of IRE1. Mol Cell. 2010;38:291-304 pubmed publisher
    ..UPR) is initiated by sequence-specific cleavage of the HAC1/XBP1 mRNA by the ER stress-induced kinase-endonuclease IRE1. We have discovered that the flavonol quercetin activates yeast IRE1's RNase and potentiates activation by ADP, a ..
  13. Okamura K, Kimata Y, Higashio H, Tsuru A, Kohno K. Dissociation of Kar2p/BiP from an ER sensory molecule, Ire1p, triggers the unfolded protein response in yeast. Biochem Biophys Res Commun. 2000;279:445-50 pubmed
    ..This negative autoregulation is similar to the regulation of mammalian cytosolic chaperone Hsp70 via its interaction with heat shock factor 1. ..
  14. Cox J, Shamu C, Walter P. Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase. Cell. 1993;73:1197-206 pubmed
    ..We have identified a gene in S. cerevisiae, IRE1, that is required for this pathway: ire1- mutants cannot activate transcription of KAR2 and PDI1, which encode the ..
  15. Kimata Y, Oikawa D, Shimizu Y, Ishiwata Kimata Y, Kohno K. A role for BiP as an adjustor for the endoplasmic reticulum stress-sensing protein Ire1. J Cell Biol. 2004;167:445-56 pubmed
    In the unfolded protein response, the type I transmembrane protein Ire1 transmits an endoplasmic reticulum (ER) stress signal to the cytoplasm...
  16. Sidrauski C, Walter P. The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response. Cell. 1997;90:1031-9 pubmed
    ..The addition of purified tRNA ligase completes splicing; we therefore have reconstituted HAC1 mRNA splicing in vitro from purified components. ..
  17. De Souza E, Pimentel F, Machado C, Martins L, da Silva W, Montero Lomel M, et al. The unfolded protein response has a protective role in yeast models of classic galactosemia. Dis Model Mech. 2014;7:55-61 pubmed publisher
    ..These results indicate that endoplasmic reticulum stress is induced under galactosemic conditions and underscores the importance of the unfolded protein response pathway to cellular adaptation in these models of classic galactosemia...
  18. Ishiwata Kimata Y, Promlek T, Kohno K, Kimata Y. BiP-bound and nonclustered mode of Ire1 evokes a weak but sustained unfolded protein response. Genes Cells. 2013;18:288-301 pubmed publisher
    ..the endoplasmic reticulum (ER)-located molecular chaperone BiP is associated with an ER-membrane protein Ire1 to inhibit its self-association...
  19. Brickner J, Walter P. Gene recruitment of the activated INO1 locus to the nuclear membrane. PLoS Biol. 2004;2:e342 pubmed
    ..Gene recruitment to the nuclear periphery, therefore, is a dynamic process and appears to play an important regulatory role. ..
  20. Nikawa J, Akiyoshi M, Hirata S, Fukuda T. Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression. Nucleic Acids Res. 1996;24:4222-6 pubmed
    The Saccharomyces cerevisiae IRE1 gene, encoding a putative receptor-type protein kinase, is known to be required for inositol prototrophy and for the induction of a chaperon molecule, BiP, encoded by KAR2, under stress conditions such as ..
  21. Cui H, Liu X, McCormick M, Wasko B, Zhao W, He X, et al. PMT1 deficiency enhances basal UPR activity and extends replicative lifespan of Saccharomyces cerevisiae. Age (Dordr). 2015;37:9788 pubmed publisher
    ..Furthermore, the increased RLS of the pmt1Δ strain could be completely abolished by deletion of either IRE1 or HAC1, two upstream modulators of the UPR...
  22. Ofiteru A, Ruta L, Rotaru C, Dumitru I, Ene C, Neagoe A, et al. Overexpression of the PHO84 gene causes heavy metal accumulation and induces Ire1p-dependent unfolded protein response in Saccharomyces cerevisiae cells. Appl Microbiol Biotechnol. 2012;94:425-35 pubmed publisher
    ..triggered the Ire1p-dependent unfolded protein response, abundant plasma membrane Pho84p could be achieved only in ire1? cells...
  23. Welihinda A, Tirasophon W, Kaufman R. The transcriptional co-activator ADA5 is required for HAC1 mRNA processing in vivo. J Biol Chem. 2000;275:3377-81 pubmed
    ..Although HAC1 mRNA requires only Ire1p and Rlg1p in vitro, we demonstrate that ADA5 is required for the IRE1/RLG1-dependent splicing reaction of HAC1 mRNA in vivo. In addition, Ada5p interacts with Ire1p...
  24. Higashio H, Kohno K. A genetic link between the unfolded protein response and vesicle formation from the endoplasmic reticulum. Biochem Biophys Res Commun. 2002;296:568-74 pubmed
    ..Overexpression of another UPR-related gene IRE1, which encodes an ER-resident transmembrane protein kinase/ribonuclease, also suppressed the growth defect of the ..
  25. Scrimale T, Didone L, de Mesy Bentley K, Krysan D. The unfolded protein response is induced by the cell wall integrity mitogen-activated protein kinase signaling cascade and is required for cell wall integrity in Saccharomyces cerevisiae. Mol Biol Cell. 2009;20:164-75 pubmed publisher
    ..Null mutation of IRE1, double mutation of ERAD components (hrd1Delta and ubc7Delta) and ire1Delta, or expression of misfolded proteins ..
  26. Taxis C, Vogel F, Wolf D. ER-golgi traffic is a prerequisite for efficient ER degradation. Mol Biol Cell. 2002;13:1806-18 pubmed
  27. Niwa M, Patil C, DeRisi J, Walter P. Genome-scale approaches for discovering novel nonconventional splicing substrates of the Ire1 nuclease. Genome Biol. 2005;6:R3 pubmed
    ..The signal from the ER lumen is transmitted by the ER-transmembrane kinase Ire1, which upon activation displays a site-specific endoribonuclease activity...
  28. Shechtman C, Henneberry A, Seimon T, Tinkelenberg A, Wilcox L, Lee E, et al. Loss of subcellular lipid transport due to ARV1 deficiency disrupts organelle homeostasis and activates the unfolded protein response. J Biol Chem. 2011;286:11951-9 pubmed publisher
    ..b>IRE1, encoding the unfolded protein sensor in the ER lumen, exhibits a lethal genetic interaction with ARV1, indicating ..
  29. Goder V, Carvalho P, Rapoport T. The ER-associated degradation component Der1p and its homolog Dfm1p are contained in complexes with distinct cofactors of the ATPase Cdc48p. FEBS Lett. 2008;582:1575-80 pubmed publisher
    ..These data suggest distinct functions for the Der1p and Dfm1p complexes. ..
  30. Labunskyy V, Gerashchenko M, Delaney J, Kaya A, Kennedy B, Kaeberlein M, et al. Lifespan extension conferred by endoplasmic reticulum secretory pathway deficiency requires induction of the unfolded protein response. PLoS Genet. 2014;10:e1004019 pubmed publisher
    ..These findings demonstrate that the UPR is an important determinant of lifespan that governs ER stress and identify a signaling network that couples stress resistance to longevity...
  31. Scott C, Kruse K, Schmidt B, Perlmutter D, McCracken A, Brodsky J. ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly. Mol Biol Cell. 2007;18:3776-87 pubmed
    ..encoding Add66p and Ire1p, a transducer of the unfolded protein response, and yeast deleted for both ADD66 and/or IRE1 accumulate polyubiquitinated proteins...
  32. Carla Famá M, Raden D, Zacchi N, Lemos D, Robinson A, Silberstein S. The Saccharomyces cerevisiae YFR041C/ERJ5 gene encoding a type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum. Biochim Biophys Acta. 2007;1773:232-42 pubmed
    ..We found that loss of the non-essential ERJ5 gene leads to a constitutively induced UPR, indicating that ERJ5 is required for maintenance of an optimal folding environment in the yeast ER. ..
  33. Ferrer Dalmau J, Randez Gil F, Marquina M, Prieto J, Casamayor A. Protein kinase Snf1 is involved in the proper regulation of the unfolded protein response in Saccharomyces cerevisiae. Biochem J. 2015;468:33-47 pubmed publisher
    ..a sustained HAC1 mRNA splicing and KAR2 mRNA levels during the recovery phase of the UPR, and dysregulation of the Ire1-oligomeric equilibrium...
  34. Chang H, Jesch S, Gaspar M, Henry S. Role of the unfolded protein response pathway in secretory stress and regulation of INO1 expression in Saccharomyces cerevisiae. Genetics. 2004;168:1899-913 pubmed
    ..Indeed, many of the Sec(-) mutants that had elevated UPR expression at semipermissive growth temperatures failed to achieve wild-type levels of INO1 expression under these same conditions. ..
  35. Liu Y, Chang A. Heat shock response relieves ER stress. EMBO J. 2008;27:1049-59 pubmed publisher
    ..Genomic analysis of HSR targets reveals that >25% have function in common with UPR targets. We propose that HSR can relieve stress in UPR-deficient cells by affecting multiple ER activities. ..
  36. Welihinda A, Tirasophon W, Green S, Kaufman R. Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p. Proc Natl Acad Sci U S A. 1997;94:4289-94 pubmed
    ..transmembrane receptor kinase, Ire1p (Ern1p), initiates signaling, although the mediators immediately downstream of Ire1 kinase are unknown...
  37. Umebayashi K, Hirata A, Horiuchi H, Ohta A, Takagi M. Unfolded protein response-induced BiP/Kar2p production protects cell growth against accumulation of misfolded protein aggregates in the yeast endoplasmic reticulum. Eur J Cell Biol. 1999;78:726-38 pubmed
    ..To investigate the roles of the UPR against the delta(pro) aggregates, we constructed an IRE1-deleted ((delta)ire1) strain...
  38. de Ruijter J, Frey A. Analysis of antibody production in Saccharomyces cerevisiae: effects of ER protein quality control disruption. Appl Microbiol Biotechnol. 2015;99:9061-71 pubmed publisher
    ..by deletion of the HTM1, YOS9, HRD1, HRD3, or UBC7 gene, with or without a disruption of the UPR by deletion of the IRE1 gene. All deletion strains were viable and did not exhibit a growth phenotype under normal growth conditions...
  39. Waller D, Jansen G, Golizeh M, Martel Lorion C, Dejgaard K, Shiao T, et al. A Covalent Cysteine-Targeting Kinase Inhibitor of Ire1 Permits Allosteric Control of Endoribonuclease Activity. Chembiochem. 2016;17:843-51 pubmed publisher
    The unfolded protein response (UPR) initiated by the transmembrane kinase/ribonuclease Ire1 has been implicated in a variety of diseases...
  40. Tyson J, Stirling C. LHS1 and SIL1 provide a lumenal function that is essential for protein translocation into the endoplasmic reticulum. EMBO J. 2000;19:6440-52 pubmed
    ..We conclude that the IRE1-dependent induction of SIL1 is a vital adaptation in Deltalhs1 cells, and that the activities associated with the ..
  41. Welihinda A, Kaufman R. The unfolded protein response pathway in Saccharomyces cerevisiae. Oligomerization and trans-phosphorylation of Ire1p (Ern1p) are required for kinase activation. J Biol Chem. 1996;271:18181-7 pubmed
    ..The activated kinase may transmit the unfolded protein signal from the ER to the nucleus to activate the transcription of the chaperone genes in the nucleus. ..
  42. Nikawa J, Yamashita S. IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae. Mol Microbiol. 1992;6:1441-6 pubmed
    A novel gene, IRE1, of Saccharomyces cerevisiae was cloned through genetic complementation of a myoinositol auxotrophic mutant...
  43. Sato M, Sato K, Nakano A. Evidence for the intimate relationship between vesicle budding from the ER and the unfolded protein response. Biochem Biophys Res Commun. 2002;296:560-7 pubmed
    ..In an effort to identify genes that positively regulate Sec12p, we isolated IRE1 as a novel multicopy suppressor of the temperature-sensitive sec12-4 mutant...
  44. Adhikari H, Vadaie N, Chow J, Caccamise L, Chavel C, Li B, et al. Role of the unfolded protein response in regulating the mucin-dependent filamentous-growth mitogen-activated protein kinase pathway. Mol Cell Biol. 2015;35:1414-32 pubmed publisher
    ..We speculate that the QC pathways broadly regulate signaling glycoproteins and their cognate pathways by recognizing altered glycosylation patterns that can occur in response to extrinsic cues. ..
  45. Liang X, Dickman M, Becker D. Proline biosynthesis is required for endoplasmic reticulum stress tolerance in Saccharomyces cerevisiae. J Biol Chem. 2014;289:27794-806 pubmed publisher
    ..To more broadly test the role of proline in ER stress, increased proline biosynthesis was shown to partially rescue the ER stress sensitivity of a hog1 null mutant in which the high osmolality pathway is disrupted. ..
  46. Gururaj C, Federman R, Federman R, Chang A. Orm proteins integrate multiple signals to maintain sphingolipid homeostasis. J Biol Chem. 2013;288:20453-63 pubmed publisher
    ..Conversely, the calcium- and calcineurin-dependent pathway signals ER stress response upon lipid dysregulation in the absence of the Orm proteins to restore ER homeostasis. ..
  47. Beh C, Rose M. Two redundant systems maintain levels of resident proteins within the yeast endoplasmic reticulum. Proc Natl Acad Sci U S A. 1995;92:9820-3 pubmed
    ..The second system is controlled by the ER membrane-bound kinase encoded by IRE1. We conclude that IRE1 and ERD2 together maintain normal concentrations of resident proteins within the ER.
  48. Liu M, Huang C, Polu S, Schneiter R, Chang A. Regulation of sphingolipid synthesis through Orm1 and Orm2 in yeast. J Cell Sci. 2012;125:2428-35 pubmed publisher
    ..Thus, sphingolipid synthesis and the UPR are coordinately regulated. ..
  49. Mannan M, Shadrick W, Biener G, Shin B, Anshu A, Raicu V, et al. An ire1-phk1 chimera reveals a dispensable role of autokinase activity in endoplasmic reticulum stress response. J Mol Biol. 2013;425:2083-99 pubmed publisher
    The endoplasmic reticulum transmembrane receptor Ire1 senses over-accumulation of unfolded proteins in the endoplasmic reticulum and initiates the unfolded protein response (UPR)...
  50. Promlek T, Ishiwata Kimata Y, Shido M, Sakuramoto M, Kohno K, Kimata Y. Membrane aberrancy and unfolded proteins activate the endoplasmic reticulum stress sensor Ire1 in different ways. Mol Biol Cell. 2011;22:3520-32 pubmed publisher
    ..Consistent with previous in vitro studies of the ER-luminal domain of the mutant UPR initiator Ire1, our study show its association with a model unfolded protein in yeast cells...
  51. Wu X, Li L, Jiang H. Doa1 targets ubiquitinated substrates for mitochondria-associated degradation. J Cell Biol. 2016;213:49-63 pubmed publisher
    ..Collectively, our results demonstrate the essential role of the Doa1-Cdc48(-Ufd1-Npl4)complex in mitochondrial proteostasis and suggest that Doa1 plays dual roles on the Cdc48 complex. ..
  52. Hitt R, Wolf D. DER7, encoding alpha-glucosidase I is essential for degradation of malfolded glycoproteins of the endoplasmic reticulum. FEMS Yeast Res. 2004;4:815-20 pubmed
    ..2.1.106) of the ER and demonstrate that its inactivity, due to a substitution of the conserved glycine residue at position 725 by arginine (G725R) in the der7-1 mutant, leads to ER-stress. ..
  53. Foresti O, Rodríguez Vaello V, Funaya C, Carvalho P. Quality control of inner nuclear membrane proteins by the Asi complex. Science. 2014;346:751-5 pubmed publisher
    ..Asi-mediated ERAD was required for ER homeostasis, which suggests that spatial segregation of protein quality control systems contributes to ER function. ..
  54. Bleve G, Di Sansebastiano G, Grieco F. Over-expression of functional Saccharomyces cerevisiae GUP1, induces proliferation of intracellular membranes containing ER and Golgi resident proteins. Biochim Biophys Acta. 2011;1808:733-44 pubmed publisher
    ..To our knowledge, we presented the first evidence of sub cellular modifications in response over-expression of a GPI-anchor remodelase in S. cerevisiae. ..
  55. Welihinda A, Tirasophon W, Green S, Kaufman R. Protein serine/threonine phosphatase Ptc2p negatively regulates the unfolded-protein response by dephosphorylating Ire1p kinase. Mol Cell Biol. 1998;18:1967-77 pubmed
    ..These results demonstrate that Ptc2p downregulates the UPR by dephosphorylating Ire1p and reveal a novel mechanism of regulation in the UPR pathway upstream of the HAC1 mRNA splicing event. ..
  56. Nguyen T, Kohno K, Kimata Y. Zinc depletion activates the endoplasmic reticulum-stress sensor Ire1 via pleiotropic mechanisms. Biosci Biotechnol Biochem. 2013;77:1337-9 pubmed
    ..Here we show that in yeast cells, the ER-stress sensor Ire1 was activated upon zinc deficiency...
  57. Kumar C, Igbaria A, D Autréaux B, Planson A, Junot C, Godat E, et al. Glutathione revisited: a vital function in iron metabolism and ancillary role in thiol-redox control. EMBO J. 2011;30:2044-56 pubmed publisher
  58. Adhikari H, Cullen P. Metabolic respiration induces AMPK- and Ire1p-dependent activation of the p38-Type HOG MAPK pathway. PLoS Genet. 2014;10:e1004734 pubmed publisher
    ..Thus, an evolutionarily conserved regulatory axis links metabolic respiration and AMPK to Ire1p, which regulates a differentiation response involving the modulated activity of ERK and p38 MAPK pathways. ..
  59. Miller K, Didone L, Krysan D. Extracellular secretion of overexpressed glycosylphosphatidylinositol-linked cell wall protein Utr2/Crh2p as a novel protein quality control mechanism in Saccharomyces cerevisiae. Eukaryot Cell. 2010;9:1669-79 pubmed publisher
    ..on Sec18p, indicating that it requires ER-to-Golgi trafficking, and accordingly, Crh2-HA accumulates in the ER in ire1? and bst1? mutants by cycloheximide chase experiments...
  60. Ishiwata Kimata Y, Yamamoto Y, Takizawa K, Kohno K, Kimata Y. F-actin and a type-II myosin are required for efficient clustering of the ER stress sensor Ire1. Cell Struct Funct. 2013;38:135-43 pubmed
    Endoplasmic reticulum (ER) stress causes the ER-resident transmembrane protein Ire1 to self-associate, leading to the formation of large oligomeric clusters...
  61. Hsu J, Tang P, Wang I, Liu C, Chen W, Tsai P, et al. Unfolded protein response regulates yeast small GTPase Arl1p activation at late Golgi via phosphorylation of Arf GEF Syt1p. Proc Natl Acad Sci U S A. 2016;113:E1683-90 pubmed publisher
    ..Thus, these findings clarify how the UPR influences the roles of Syt1p, Arl1p, and Imh1p in Golgi transport. ..
  62. Schroder M, Clark R, Kaufman R. IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast. Mol Microbiol. 2003;49:591-606 pubmed
    ..Here, we demonstrate that in addition to this well-understood ER stress signalling pathway, a second, IRE1, HAC1 and UPRE-independent mechanism for transcriptional activation upon ER stress, exists in yeast...
  63. Bailly Bechet M, Borgs C, Braunstein A, Chayes J, Dagkessamanskaia A, Francois J, et al. Finding undetected protein associations in cell signaling by belief propagation. Proc Natl Acad Sci U S A. 2011;108:882-7 pubmed publisher
    ..The algorithm we present is specially suited for very large datasets, can run in parallel, and can be adapted to other problems in systems biology. On renowned benchmarks it outperforms other algorithms in the field. ..
  64. Halbleib K, Pesek K, Covino R, Hofbauer H, Wunnicke D, Hänelt I, et al. Activation of the Unfolded Protein Response by Lipid Bilayer Stress. Mol Cell. 2017;67:673-684.e8 pubmed publisher
    ..We show that the most conserved transducer of ER stress, Ire1, uses an amphipathic helix (AH) to sense membrane aberrancies and control UPR activity...