INP53

Summary

Gene Symbol: INP53
Description: phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Alias: SJL3, SOP2, phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
Species: Saccharomyces cerevisiae S288c
Products:     INP53

Top Publications

  1. Srinivasan S, Seaman M, Nemoto Y, Daniell L, Suchy S, Emr S, et al. Disruption of three phosphatidylinositol-polyphosphate 5-phosphatase genes from Saccharomyces cerevisiae results in pleiotropic abnormalities of vacuole morphology, cell shape, and osmohomeostasis. Eur J Cell Biol. 1997;74:350-60 pubmed
    ..No defect in carboxypeptidase Y sorting was seen in a processing and targeting assay. Abnormal actin cytoskeleton morphology was present in some of the strains carrying mutations in two of the genes. ..
  2. Singer Krüger B, Nemoto Y, Daniell L, Ferro Novick S, De Camilli P. Synaptojanin family members are implicated in endocytic membrane traffic in yeast. J Cell Sci. 1998;111 ( Pt 22):3347-56 pubmed
    ..The genome of Saccharomyces cerevisiae contains three synaptojanin-like genes (SJL1, SJL2 and SJL3), none of which is essential for growth...
  3. Stefan C, Audhya A, Emr S. The yeast synaptojanin-like proteins control the cellular distribution of phosphatidylinositol (4,5)-bisphosphate. Mol Biol Cell. 2002;13:542-57 pubmed
    ..Taken together, our results demonstrate that the yeast synaptojanins control the localization of PtdIns(4,5)P(2) in vivo and provide further evidence for the compartmentalization of different PI species. ..
  4. Stolz L, Huynh C, Thorner J, York J. Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae. Genetics. 1998;148:1715-29 pubmed
    ..Here we describe two related yeast loci, INP52 (YNL106c) and INP53 (YOR109w)...
  5. Larsson K, Bohl F, Sjöström I, Akhtar N, Strand D, Mechler B, et al. The Saccharomyces cerevisiae SOP1 and SOP2 genes, which act in cation homeostasis, can be functionally substituted by the Drosophila lethal(2)giant larvae tumor suppressor gene. J Biol Chem. 1998;273:33610-8 pubmed
    ..Deletion of SOP2, encoding a protein sharing 54% amino acid identity with Sop1p, produced only slight Na+ sensitivity...
  6. Zhong R, Burk D, Morrison W, Ye Z. FRAGILE FIBER3, an Arabidopsis gene encoding a type II inositol polyphosphate 5-phosphatase, is required for secondary wall synthesis and actin organization in fiber cells. Plant Cell. 2004;16:3242-59 pubmed
    ..Together, our findings suggest that the FRA3 type II 5PTase is involved in phosphoinositide metabolism and influences secondary wall synthesis and actin organization. ..
  7. Bensen E, Costaguta G, Payne G. Synthetic genetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae. Roles for synaptojanin-like Inp53p and dynamin-related Vps1p in clathrin-dependent protein sorting at the trans-Golgi network. Genetics. 2000;154:83-97 pubmed
    ..in ribosome biogenesis; LUV1, encoding a product potentially involved in vacuole and microtubule organization; and INP53, encoding a synaptojanin-like inositol polyphosphate 5-phosphatase...
  8. Luo W, Chang A. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol. 1997;138:731-46 pubmed
    ..One of these strains defective in Kex2 trafficking carries a mutation in SOP2, encoding a homologue of mammalian synaptojanin (implicated in synaptic vesicle endocytosis and recycling)...
  9. Ha S, Bunch J, Hama H, DeWald D, Nothwehr S. A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein. Mol Biol Cell. 2001;12:3175-90 pubmed
    ..A screen for genes required for the slow delivery mechanism uncovered INP53, a gene encoding a phosphoinositide phosphatase...

More Information

Publications22

  1. Fröhlich F, Christiano R, Olson D, Alcazar Roman A, DECAMILLI P, Walther T. A role for eisosomes in maintenance of plasma membrane phosphoinositide levels. Mol Biol Cell. 2014;25:2797-806 pubmed publisher
    ..and function of Inp51 but not for the homologous phosphatidylinositol bisphosphate phosphatases Inp52/Sjl2 and Inp53/Sjl3. Consistent with this, absence of Pil1 increases total and available PI(4,5)P2 levels at the plasma membrane...
  2. Ha S, Torabinejad J, DeWald D, Wenk M, Lucast L, De Camilli P, et al. The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway. Mol Biol Cell. 2003;14:1319-33 pubmed
    ..Unlike chc1 mutants, inp53 mutants do not mislocalize TGN proteins to the cell surface, consistent with the idea that Chc1p and Inp53p act at ..
  3. Böttcher C, Wicky S, Schwarz H, Singer Krüger B. Sjl2p is specifically involved in early steps of endocytosis intimately linked to actin dynamics via the Ark1p/Prk1p kinases. FEBS Lett. 2006;580:633-41 pubmed
    ..Consistent with the finding that Sjl2p can bind to clathrin heavy chain in vitro, our results suggest that Sjl2p localizes to smooth endocytic vesicles that may be derived from clathrin-coated structures. ..
  4. Hughes W, Woscholski R, Cooke F, Patrick R, Dove S, McDonald N, et al. SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases. J Biol Chem. 2000;275:801-8 pubmed
    ..It is concluded that SAC1 encodes a novel lipid phosphoinositide phosphatase in which specific mutations can cause the sac1 phenotypes by altering the in vivo regulation of the protein rather than by destroying phosphatase activity. ..
  5. Guo S, Stolz L, Lemrow S, York J. SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J Biol Chem. 1999;274:12990-5 pubmed
    ..Additionally, the tethering of PPIPase and 5-phosphatase activities indicate a novel mechanism by which concerted phosphoinositide hydrolysis participates in membrane trafficking. ..
  6. Parrish W, Stefan C, Emr S. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J Cell Sci. 2005;118:5589-601 pubmed
    ..Taken together, the results of this study indicated that aberrant Rho1p/Pkc1p signaling contributes to the lethal effects of PtdIns(3)P accumulation in cells deficient in PI 3-phosphatase activity. ..
  7. Nguyen P, Hasek J, Kohlwein S, Romero C, Choi J, Vancura A. Interaction of Pik1p and Sjl proteins in membrane trafficking. FEMS Yeast Res. 2005;5:363-71 pubmed
    ..Deletion of SJL3 gene is synthetically lethal with pik1ts, and deletions of SJL1 or SJL2 genes in pik1ts cells exacerbate the ..
  8. Saiz J, Santos M, Vazquez de Aldana C, Revuelta J. Disruption of six unknown open reading frames from Saccharomyces cerevisiae reveals two genes involved in vacuolar morphogenesis and one gene required for sporulation. Yeast. 1999;15:155-64 pubmed
    ..Strains bearing single and double deletions of YNL106c and YOR109w were seen to display abnormal vacuolar morphologies of varying degrees. Complementation tests indicated that YNL106c and YOR109w are redundant genes. ..
  9. Ooms L, McColl B, Wiradjaja F, Wijayaratnam A, Gleeson P, Gething M, et al. The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations. Mol Cell Biol. 2000;20:9376-90 pubmed
    ..This may provide a mechanism for regulating actin polymerization and cell growth as an acute adaptive response to hyperosmotic stress. ..
  10. Wicky S, Frischmuth S, Singer Krüger B. Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast. FEBS Lett. 2003;537:35-41 pubmed
    ..We propose that Bsp1p is an adapter that links Sjl2p and Sjl3p to the cortical actin cytoskeleton. ..
  11. Zhong R, Ye Z. The SAC domain-containing protein gene family in Arabidopsis. Plant Physiol. 2003;132:544-55 pubmed
    ..Moreover, the expression of the AtSAC6 gene was highly induced by salinity. These results provide a foundation for future studies on the elucidation of the cellular functions of SAC domain-containing proteins in Arabidopsis. ..
  12. Guiney E, Goldman A, Elias J, Cyert M. Calcineurin regulates the yeast synaptojanin Inp53/Sjl3 during membrane stress. Mol Biol Cell. 2015;26:769-85 pubmed publisher
    ..Hyperosmotic shock promotes calcineurin binding to and dephosphorylation of the PI(4,5)P2 phosphatase synaptojanin/Inp53/Sjl3 and causes dramatic calcineurin-dependent reorganization of PI(4,5)P2-enriched membrane domains...
  13. Foti M, Audhya A, Emr S. Sac1 lipid phosphatase and Stt4 phosphatidylinositol 4-kinase regulate a pool of phosphatidylinositol 4-phosphate that functions in the control of the actin cytoskeleton and vacuole morphology. Mol Biol Cell. 2001;12:2396-411 pubmed
    ..Regulation of this PtdIns(4)P pool appears to be crucial for the maintenance of vacuole morphology, regulation of lipid storage, Golgi function, and actin cytoskeleton organization. ..