Gene Symbol: INH1
Description: ATPase inhibitor
Alias: ATPase inhibitor
Species: Saccharomyces cerevisiae S288c
Ichikawa N, Mizuno M. Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif. 2004;37:97-101 pubmed
..import signal sequence was replaced by a sequence containing the import signal sequence of a mitochondrial ATPase inhibitor, its processing site, and six consecutive histidines...
Corvest V, Sigalat C, Venard R, Falson P, Mueller D, Haraux F. The binding mechanism of the yeast F1-ATPase inhibitory peptide: role of catalytic intermediates and enzyme turnover. J Biol Chem. 2005;280:9927-36 pubmed
..A model is proposed that suggests that IF1 binds rapidly, but loosely to F(1) with two or three catalytic sites filled, and is then locked in the enzyme during catalytic hydrolysis of ATP. ..
Andrianaivomananjaona T, Moune Dimala M, Herga S, David V, Haraux F. How the N-terminal extremity of Saccharomyces cerevisiae IF1 interacts with ATP synthase: a kinetic approach. Biochim Biophys Acta. 2011;1807:197-204 pubmed publisher
..Moreover, the data obtained using chimeric PsaE-IF1 suggest that before binding IF1 presents to the enzyme with its middle part facing a catalytic interface and its Nter extremity folded in the opposite direction. ..
Hashimoto T, Yoshida Y, Tagawa K. Binding properties of an intrinsic ATPase inhibitor and occurrence in yeast mitochondria of a protein factor which stabilizes and facilitates the binding of the inhibitor to F1F0-ATPase. J Biochem. 1983;94:715-20 pubmed
The content of an intrinsic ATPase inhibitor in mitochondria was determined by a radioimmunoassay procedure which showed the molar ratio of the inhibitor to ATPase to be 1:1...
Arnold I, Pfeiffer K, Neupert W, Stuart R, Schagger H. ATP synthase of yeast mitochondria. Isolation of subunit j and disruption of the ATP18 gene. J Biol Chem. 1999;274:36-40 pubmed
..In the absence of Su j, spontaneously arising rho- cells were observed that lacked also ubiquinol-cytochrome c reductase and cytochrome c oxidase activities. We conclude that Su j is a novel and essential subunit of yeast ATP synthase. ..
Dienhart M, Pfeiffer K, Schagger H, Stuart R. Formation of the yeast F1F0-ATP synthase dimeric complex does not require the ATPase inhibitor protein, Inh1. J Biol Chem. 2002;277:39289-95 pubmed
..Here we have directly addressed the involvement of the yeast inhibitor protein, Inh1, and its known accessory proteins, Stf1 and Stf2, in the formation of the yeast F1F0-ATP synthase dimer...
Kominsky D, Brownson M, Updike D, Thorsness P. Genetic and biochemical basis for viability of yeast lacking mitochondrial genomes. Genetics. 2002;162:1595-604 pubmed
..In contrast, deletion of INH1, which encodes an inhibitor of the F(1)F(0)-ATPase, partially suppressed the growth defect of yme1 yeast lacking ..
Venard R, Brèthes D, Giraud M, Vaillier J, Velours J, Haraux F. Investigation of the role and mechanism of IF1 and STF1 proteins, twin inhibitory peptides which interact with the yeast mitochondrial ATP synthase. Biochemistry. 2003;42:7626-36 pubmed