IDH1

Summary

Gene Symbol: IDH1
Description: isocitrate dehydrogenase (NAD(+)) IDH1
Alias: isocitrate dehydrogenase (NAD(+)) IDH1
Species: Saccharomyces cerevisiae S288c
Products:     IDH1

Top Publications

  1. Zhao W, McAlister Henn L. Assembly and function of a cytosolic form of NADH-specific isocitrate dehydrogenase in yeast. J Biol Chem. 1996;271:10347-52 pubmed
    ..Yeast isocitrate dehydrogenase is an octomer composed of two subunits (IDH1 and IDH2) encoded by different genes and possessing independent mitochondrial targeting presequences...
  2. Panisko E, McAlister Henn L. Subunit interactions of yeast NAD+-specific isocitrate dehydrogenase. J Biol Chem. 2001;276:1204-10 pubmed
    ..isocitrate dehydrogenase is an octamer composed of four each of two nonidentical but related subunits designated IDH1 and IDH2...
  3. Rezaei M, Aslankoohi E, Verstrepen K, Courtin C. Contribution of the tricarboxylic acid (TCA) cycle and the glyoxylate shunt in Saccharomyces cerevisiae to succinic acid production during dough fermentation. Int J Food Microbiol. 2015;204:24-32 pubmed publisher
    ..Similarly, double deletion of IDH1 and IDP1 decreased succinic acid production by 85%, while also affecting the fermentation rate...
  4. Chen X, Wang X, Kaufman B, Butow R. Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science. 2005;307:714-7 pubmed
    ..When constitutively expressed, Aco1p can replace the mtDNA packaging function of the high-mobility-group protein Abf2p. Thus, Aco1p may integrate metabolic signals and mtDNA maintenance. ..
  5. Hu G, McAlister Henn L. Novel allosteric properties produced by residue substitutions in the subunit interface of yeast NAD+-specific isocitrate dehydrogenase. Arch Biochem Biophys. 2006;453:207-16 pubmed
    Yeast NAD+-specific isocitrate dehydrogenase (IDH) is an octamer of four IDH1 and four IDH2 subunits, and the basic structural unit of the enzyme is an IDH1/IDH2 heterodimer...
  6. Minard K, McAlister Henn L. Redox responses in yeast to acetate as the carbon source. Arch Biochem Biophys. 2009;483:136-43 pubmed publisher
    ..Long-term chronological lifespans of the parental and idhDelta strains were similar, but viability of the mutant strain was dependent on both pathways for NAD(H) biosynthesis. ..
  7. Przybyla Zawislak B, Gadde D, Ducharme K, McCammon M. Genetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes. Genetics. 1999;152:153-66 pubmed
    ..The only other TCA cycle gene to display the glycerol-suppressor-accumulation phenotype was IDH1, which encodes the companion Idh1p subunit of NAD-IDH...
  8. Hu G, Lin A, McAlister Henn L. Physiological consequences of loss of allosteric activation of yeast NAD+-specific isocitrate dehydrogenase. J Biol Chem. 2006;281:16935-42 pubmed
    ..This suggests an important physiological role for allosteric activation of IDH during changes in environmental conditions. ..
  9. Taylor A, Hu G, Hart P, McAlister Henn L. Allosteric motions in structures of yeast NAD+-specific isocitrate dehydrogenase. J Biol Chem. 2008;283:10872-80 pubmed publisher
    ..for ligand binding to homologous but distinct regulatory and catalytic sites positioned at the interfaces between IDH1 and IDH2 subunits and define pathways of communication between heterodimers and heterotetramers in the hetero-..

More Information

Publications20

  1. Song P, Wei H, Cao Z, Wang P, Zhu G. Single arginine mutation in two yeast isocitrate dehydrogenases: biochemical characterization and functional implication. PLoS ONE. 2014;9:e115025 pubmed publisher
    ..Mutation of the catalytic Arg132 residue of human IDH1 (HcIDH) eliminates the enzyme's wild-type isocitrate oxidation activity, but confer the mutant an ability of ..
  2. Lin A, McAlister Henn L. Basis for half-site ligand binding in yeast NAD(+)-specific isocitrate dehydrogenase. Biochemistry. 2011;50:8241-50 pubmed publisher
    ..regulated octameric enzyme composed of four heterodimers of a catalytic IDH2 subunit and a regulatory IDH1 subunit...
  3. McAlister Henn L, Small W. Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol. 1997;57:317-39 pubmed
  4. Lin A, Demeler B, Minard K, Anderson S, Schirf V, Galaleldeen A, et al. Construction and analyses of tetrameric forms of yeast NAD+-specific isocitrate dehydrogenase. Biochemistry. 2011;50:230-9 pubmed
    ..NAD(+)-specific isocitrate dehydrogenase (IDH) is an octameric enzyme composed of four heterodimers of regulatory IDH1 and catalytic IDH2 subunits...
  5. Helbig A, de Groot M, van Gestel R, Mohammed S, de Hulster E, Luttik M, et al. A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics. 2009;9:4787-98 pubmed publisher
    ..In addition, this latter approach allowed screening for possible novel complex interaction partners, since for example protein Aim38p, with a yet unknown function, was identified as a possible component of respiratory chain complex IV. ..
  6. Garcia J, Minard K, Lin A, McAlister Henn L. Disulfide bond formation in yeast NAD+-specific isocitrate dehydrogenase. Biochemistry. 2009;48:8869-78 pubmed publisher
    ..dehydrogenase (IDH) of Saccharomyces cerevisiae is an octameric enzyme composed of four heterodimers of regulatory IDH1 and catalytic IDH2 subunits...
  7. Hess D, Myers C, Huttenhower C, Hibbs M, Hayes A, Paw J, et al. Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis. PLoS Genet. 2009;5:e1000407 pubmed publisher
    ..Our results demonstrate that by leveraging computational analysis to direct quantitative experimental assays, we have characterized mutants with subtle mitochondrial defects whose phenotypes were undetected by high-throughput methods. ..
  8. Kucej M, Kucejova B, SUBRAMANIAN R, Chen X, Butow R. Mitochondrial nucleoids undergo remodeling in response to metabolic cues. J Cell Sci. 2008;121:1861-8 pubmed publisher
    ..A mutant form of Hsp60 that causes mtDNA instability has altered submitochondrial localization, which suggests that nucleoid remodeling is essential for the maintenance of mitochondrial genome...
  9. Barnard E, McFerran N, Trudgett A, Nelson J, Timson D. Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method. Fungal Genet Biol. 2008;45:597-604 pubmed publisher
  10. Contreras Shannon V, Lin A, McCammon M, McAlister Henn L. Kinetic properties and metabolic contributions of yeast mitochondrial and cytosolic NADP+-specific isocitrate dehydrogenases. J Biol Chem. 2005;280:4469-75 pubmed
    ..Collectively, these results suggest an ancillary role for IDP1 in cellular glutamate synthesis and a role for IDP2 in equilibrating and maintaining cellular levels of isocitrate and alpha-ketoglutarate. ..
  11. Lin A, Hakala K, Weintraub S, McAlister Henn L. Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase. Arch Biochem Biophys. 2008;474:205-12 pubmed publisher