Gene Symbol: HXK1
Description: hexokinase 1
Alias: hexokinase 1
Species: Saccharomyces cerevisiae S288c
Products:     HXK1

Top Publications

  1. Ma H, Botstein D. Effects of null mutations in the hexokinase genes of Saccharomyces cerevisiae on catabolite repression. Mol Cell Biol. 1986;6:4046-52 pubmed
    ..We constructed null mutations in both hexokinase genes, HXK1 and HXK2, and studied their effect on the fermentation of fructose and on catabolite repression of three different ..
  2. Hohmann S, Winderickx J, de Winde J, Valckx D, Cobbaert P, Luyten K, et al. Novel alleles of yeast hexokinase PII with distinct effects on catalytic activity and catabolite repression of SUC2. Microbiology. 1999;145 ( Pt 3):703-14 pubmed
    ..The phosphorylation of glucose or fructose is catalysed by hexokinase PI (Hxk1), hexokinase PII (Hxk2) and a specific glucokinase (Glk1)...
  3. Belinchon M, Gancedo J. Different signalling pathways mediate glucose induction of SUC2, HXT1 and pyruvate decarboxylase in yeast. FEMS Yeast Res. 2007;7:40-7 pubmed
    ..The induction of HXT1 was not affected by the absence of Gpr1. In an hxk1 hxk2 glk1 strain, high glucose fully induced SUC2, caused partial induction of HXT1 and had no effect on Pdc...
  4. Pelaez R, Herrero P, Moreno F. Nuclear export of the yeast hexokinase 2 protein requires the Xpo1 (Crm1)-dependent pathway. J Biol Chem. 2009;284:20548-55 pubmed publisher
    ..Our study uncovers a new cargo for the Xpo1 yeast exportin and identifies Hxk2 phosphorylation at serine 14 as a regulatory mechanism that controls its nuclear exit in function of the glucose levels. ..
  5. Clifton D, Weinstock S, Fraenkel D. Glycolysis mutants in Saccharomyces cerevisiae. Genetics. 1978;88:1-11 pubmed
    ..In diploids the mutations were recessive. pyk, pgi, pfk, and gcr segregated 2:2 from their wild-type alleles. PYK hxk, PYK pfk, and PYK gcr segregrants grew on glucose. ..
  6. Vega M, Riera A, Fernández Cid A, Herrero P, Moreno F. Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex. J Biol Chem. 2016;291:7267-85 pubmed publisher
    ..Thus, our data indicate that Hxk2 is more intimately involved in gene regulation than previously thought. ..
  7. Walsh R, Kawasaki G, Fraenkel D. Cloning of genes that complement yeast hexokinase and glucokinase mutants. J Bacteriol. 1983;154:1002-4 pubmed
  8. Jiang H, Medintz I, Zhang B, Michels C. Metabolic signals trigger glucose-induced inactivation of maltose permease in Saccharomyces. J Bacteriol. 2000;182:647-54 pubmed
    ..to glucose, glucose catabolism, glucose transport by the Hxt transporters, or glucose phosphorylation by hexokinase 1 or 2...
  9. Maity H, Maiti N, Jarori G. Time-resolved fluorescence of tryptophans in yeast hexokinase-PI: effect of subunit dimerization and ligand binding. J Photochem Photobiol B. 2000;55:20-6 pubmed

More Information


  1. Hung G, Brown C, Wolfe A, Liu J, Chiang H. Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events. J Biol Chem. 2004;279:49138-50 pubmed
    ..By contrast, the cAMP-dependent signaling pathway was not involved in the non-vacuolar degradation of these proteins, although the phosphorylation of glucose was required. ..
  2. Lobo Z, Maitra P. Physiological role of glucose-phosphorylating enzymes in Saccharomyces cerevisiae. Arch Biochem Biophys. 1977;182:639-45 pubmed
  3. Pelaez R, Herrero P, Moreno F. Functional domains of yeast hexokinase 2. Biochem J. 2010;432:181-90 pubmed publisher
    ..The two mutants, Hxk2(wca) and Hxk2(wrf) retain single functions, as a transcriptional factor or as an enzyme with hexose-phosphorylating activity, but have lost the original bifunctionality of Hxk2. ..
  4. Ulbricht R, Olivas W. Puf1p acts in combination with other yeast Puf proteins to control mRNA stability. RNA. 2008;14:246-62 pubmed
    ..of nearly 40 candidate mRNAs, we established two of these as true mRNA targets of Puf-mediated decay in yeast, HXK1 and TIF1. In a novel finding, multiple Puf proteins, including Puf1p, regulate both of these mRNAs in combination...
  5. Gregan J, Bui D, Pillich R, Fink M, Zsurka G, Schweyen R. The mitochondrial inner membrane protein Lpe10p, a homologue of Mrs2p, is essential for magnesium homeostasis and group II intron splicing in yeast. Mol Gen Genet. 2001;264:773-81 pubmed
    ..However, they cannot easily substitute for each other. Their roles in magnesium homeostasis and, possibly as a secondary effect, in RNA splicing are discussed. ..
  6. Lobo Z, Maitra P. Genetics of yeast hexokinase. Genetics. 1977;86:727-44 pubmed
    ..Both mutant strains grow on glucose but are unable to grow on fructose, and contain two mutant genes hxk1 and hxk2 each. The mutations are recessive and noncomplementing...
  7. Belinchon M, Gancedo J. Glucose controls multiple processes in Saccharomyces cerevisiae through diverse combinations of signaling pathways. FEMS Yeast Res. 2007;7:808-18 pubmed
    We have studied how the lack of glucose sensors in the plasma membrane, or of the enzymes Hxk1, Hxk2, Glk1, which catalyze the first intracellular step in glucose metabolism, affect the different responses of Saccharomyces cerevisiae to ..
  8. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..
  9. Bianconi M. Calorimetric determination of thermodynamic parameters of reaction reveals different enthalpic compensations of the yeast hexokinase isozymes. J Biol Chem. 2003;278:18709-13 pubmed
    The change in enthalpy and rate constants for the reactions of yeast hexokinase isozymes, PI (Hxk1) and PII (Hxk2), was determined at pH 7.6 and 25 degrees C by isothermal titration calorimetry...
  10. Bisson L, Fraenkel D. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983;80:1730-4 pubmed
    ..analog 6-deoxyglucose was measured in wild-type Saccharomyces cerevisiae and two mutant strains, one (hxk1 hxk2) lacking both hexokinase A(P-I) and B(P-II) but containing glucokinase (and hence able to grow on glucose but ..