HSP60

Summary

Gene Symbol: HSP60
Description: chaperone ATPase HSP60
Alias: CPN60, MIF4, MNA2, chaperone ATPase HSP60
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Markov D, Savkina M, Anikin M, Del Campo M, Ecker K, Lambowitz A, et al. Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification. Yeast. 2009;26:423-40 pubmed publisher
    ..Further, we found that Mss116p inhibits transcription by mtRNAP in vitro in a steady-state reaction. Our results support the hypothesis that Mss116p and Pet127p are involved in modulation of mtRNAP activity. ..
  2. Martin J, Horwich A, Hartl F. Prevention of protein denaturation under heat stress by the chaperonin Hsp60. Science. 1992;258:995-8 pubmed
    ..The mitochondrial heat shock protein 60 (Hsp60) has now been shown to form complexes with a variety of polypeptides in organelles exposed to heat stress...
  3. Backes S, Hess S, Boos F, Woellhaf M, Gödel S, Jung M, et al. Tom70 enhances mitochondrial preprotein import efficiency by binding to internal targeting sequences. J Cell Biol. 2018;217:1369-1382 pubmed publisher
  4. Kalderon B, Kogan G, Bubis E, Pines O. Cytosolic Hsp60 can modulate proteasome activity in yeast. J Biol Chem. 2015;290:3542-51 pubmed publisher
    b>Hsp60, an essential oligomeric molecular mitochondrial chaperone, has been subject to rigorous basic and clinical research...
  5. Starita L, Lo R, Eng J, von Haller P, Fields S. Sites of ubiquitin attachment in Saccharomyces cerevisiae. Proteomics. 2012;12:236-40 pubmed publisher
    ..However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae. ..
  6. Huang K, Filarsky M, Padula M, Raftery M, Herbert B, Wilkins M. Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Proteomics. 2009;9:2494-502 pubmed publisher
    ..We illustrate the utility of the technique in the analysis of Saccharomyces cerevisiae cellular lysate. ..
  7. Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
    ..The association of these proteins is discussed in terms of NADH-channeling. ..
  8. Koll H, Guiard B, Rassow J, Ostermann J, Horwich A, Neupert W, et al. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell. 1992;68:1163-75 pubmed
    ..While in the matrix, the protein interacts with hsp60, which arrests its folding prior to export...
  9. Kaufman B, Newman S, Hallberg R, Slaughter C, Perlman P, Butow R. In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc Natl Acad Sci U S A. 2000;97:7772-7 pubmed
    ..Genetic experiments indicate that hsp60-ts mutants have a petite-inducing phenotype at the permissive temperature and that a kgd2Delta mutation increases ..

More Information

Publications24

  1. Chacinska A, Rehling P, Guiard B, Frazier A, Schulze Specking A, Pfanner N, et al. Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J. 2003;22:5370-81 pubmed
    ..Thus, Tim50 functions as a dynamic factor and the IMS domain of Tom22 represents a stabilizing element in formation of a productive translocation contact site supercomplex. ..
  2. Marom M, Dayan D, Demishtein Zohary K, Mokranjac D, Neupert W, Azem A. Direct interaction of mitochondrial targeting presequences with purified components of the TIM23 protein complex. J Biol Chem. 2011;286:43809-15 pubmed publisher
    ..we demonstrate, for the first time, the ability of purified Tim50IMS and Tim44 to interact directly with the yeast Hsp60 presequence...
  3. Staresincic L, Walker J, Dirac Svejstrup A, Mitter R, Svejstrup J. GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein. J Biol Chem. 2011;286:35553-61 pubmed publisher
    ..Together, our data suggest that Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase. ..
  4. Hallberg E, Shu Y, Hallberg R. Loss of mitochondrial hsp60 function: nonequivalent effects on matrix-targeted and intermembrane-targeted proteins. Mol Cell Biol. 1993;13:3050-7 pubmed
    We have created yeast strains in which the mitochondrial chaperonin, hsp60, can be either physically depleted or functionally inactivated...
  5. Cheng M, Hartl F, Horwich A. The mitochondrial chaperonin hsp60 is required for its own assembly. Nature. 1990;348:455-8 pubmed
    Heatshock protein 60 (hsp60) in the matrix of mitochondria is essential for the folding and assembly of newly imported proteins...
  6. Chen X, Wang X, Kaufman B, Butow R. Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science. 2005;307:714-7 pubmed
    ..When constitutively expressed, Aco1p can replace the mtDNA packaging function of the high-mobility-group protein Abf2p. Thus, Aco1p may integrate metabolic signals and mtDNA maintenance. ..
  7. Shia W, Osada S, Florens L, Swanson S, Washburn M, Workman J. Characterization of the yeast trimeric-SAS acetyltransferase complex. J Biol Chem. 2005;280:11987-94 pubmed
    ..This specific lysine preference corresponds to the role of SAS complex in antagonizing the spreading of Sir proteins at silent loci in S. cerevisiae. ..
  8. Mnaimneh S, Davierwala A, Haynes J, Moffat J, Peng W, Zhang W, et al. Exploration of essential gene functions via titratable promoter alleles. Cell. 2004;118:31-44 pubmed
    ..We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection. ..
  9. Rospert S, Glick B, Jeno P, Schatz G, Todd M, Lorimer G, et al. Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria. Proc Natl Acad Sci U S A. 1993;90:10967-71 pubmed
    Chaperonin 60 (cpn60) and chaperonin 10 (cpn10) constitute the chaperonin system in prokaryotes, mitochondria, and chloroplasts. In Escherichia coli, these two chaperonins are also termed groEL and groES...
  10. Ziv I, Matiuhin Y, Kirkpatrick D, Erpapazoglou Z, Leon S, Pantazopoulou M, et al. A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis. Mol Cell Proteomics. 2011;10:M111.009753 pubmed publisher
    ..We conclude that despite the shared use of the ubiquitin molecule, the two branches of the ubiquitin machinery--the ubiquitin-proteasome system and the ubiquitin trafficking system--were unevenly perturbed by expression of K0 ubiquitin. ..
  11. Schwock J, Kirchberger J, Edelmann A, Kriegel T, Kopperschläger G. Interaction of 6-phosphofructokinase with cytosolic proteins of Saccharomyces cerevisiae. Yeast. 2004;21:483-94 pubmed
    ..Western blot analysis identified the mitochondrial chaperonin Hsp60, as well as the cytoskeleton proteins alpha-tubulin and actin, in complexes with Pfk-1 that were co-precipitated ..
  12. Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
  13. Cheng M, Hartl F, Martin J, Pollock R, Kalousek F, Neupert W, et al. Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature. 1989;337:620-5 pubmed
    A nuclear encoded mitochondrial heat-shock protein hsp60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix...
  14. Böttinger L, Oeljeklaus S, Guiard B, Rospert S, Warscheid B, Becker T. Mitochondrial heat shock protein (Hsp) 70 and Hsp10 cooperate in the formation of Hsp60 complexes. J Biol Chem. 2015;290:11611-22 pubmed publisher
    ..The chaperonin Hsp60, together with its cofactor Hsp10, catalyzes folding of a subset of mtHsp70 client proteins...
  15. Dubaquie Y, Looser R, Rospert S. Significance of chaperonin 10-mediated inhibition of ATP hydrolysis by chaperonin 60. Proc Natl Acad Sci U S A. 1997;94:9011-6 pubmed
    ..We have functionally characterized the yeast mitochondrial chaperonins hsp60 and hsp10. In the presence of ADP, one molecule of hsp10 binds to hsp60 with an apparent Kd of 0...