Gene Symbol: HRR25
Description: serine/threonine protein kinase HRR25
Alias: KTI14, serine/threonine protein kinase HRR25
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Leger Silvestre I, Milkereit P, Ferreira Cerca S, Saveanu C, Rousselle J, Choesmel V, et al. The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast. EMBO J. 2004;23:2336-47 pubmed
    ..We conclude that, during the final maturation steps in the nucleus, incorporation of the ribosomal protein Rps15p is specifically required to render the pre-40S particles competent for translocation to the cytoplasm. ..
  2. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Analysis of the remaining mutants and other known gamma-toxin resistant mutants revealed that sit4, kti14, and KTI5 mutants also have a defect in the formation of mcm(5)...
  3. Petronczki M, Matos J, Mori S, Gregan J, Bogdanova A, Schwickart M, et al. Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1. Cell. 2006;126:1049-64 pubmed
    ..We show here that the monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 delta/epsilon (CK1delta/epsilon), and recruits it to meiosis I centromeres...
  4. Murakami A, Kimura K, Nakano A. The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum. J Biol Chem. 1999;274:3804-10 pubmed
    ..RST2 turned out to be identical to HRR25, a gene encoding a dual-specificity casein kinase I in yeast...
  5. Katis V, Lipp J, Imre R, Bogdanova A, Okaz E, Habermann B, et al. Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Dev Cell. 2010;18:397-409 pubmed publisher
    ..Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8. ..
  6. Mehlgarten C, Jablonowski D, Breunig K, Stark M, Schaffrath R. Elongator function depends on antagonistic regulation by casein kinase Hrr25 and protein phosphatase Sit4. Mol Microbiol. 2009;73:869-81 pubmed publisher
    ..Here we show that zymocin resistance of casein kinase hrr25 mutants associates with hypophosphorylation of Elp1 and that nonsense suppression by the Elongator-dependent SUP4 ..
  7. Pfaffenwimmer T, Reiter W, Brach T, Nogellova V, Papinski D, Schuschnig M, et al. Hrr25 kinase promotes selective autophagy by phosphorylating the cargo receptor Atg19. EMBO Rep. 2014;15:862-70 pubmed publisher
    ..We show that the essential kinase Hrr25 activates the cargo receptor Atg19 by phosphorylation, which is required to link cargo to the Atg11 scaffold, ..
  8. Ho Y, Mason S, Kobayashi R, Hoekstra M, Andrews B. Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:581-6 pubmed
    ..In this paper, we provide evidence suggesting that another kinase, Hrr25, is also involved in the transcriptional response to DNA damage through its interaction with the transcription ..
  9. Tanaka C, Tan L, Mochida K, Kirisako H, Koizumi M, Asai E, et al. Hrr25 triggers selective autophagy-related pathways by phosphorylating receptor proteins. J Cell Biol. 2014;207:91-105 pubmed publisher
    ..In this paper, we find that the highly conserved multifunctional kinase Hrr25 regulates two distinct selective autophagy-related pathways in Saccharomyces cerevisiae...

More Information


  1. Lord C, Bhandari D, Menon S, Ghassemian M, Nycz D, HAY J, et al. Sequential interactions with Sec23 control the direction of vesicle traffic. Nature. 2011;473:181-6 pubmed publisher
    ..These events are conserved in mammalian cells. ..
  2. Corbett K, Harrison S. Molecular architecture of the yeast monopolin complex. Cell Rep. 2012;1:583-9 pubmed publisher
    ..We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex...
  3. Ray P, Basu U, Ray A, Majumdar R, Deng H, Maitra U. The Saccharomyces cerevisiae 60 S ribosome biogenesis factor Tif6p is regulated by Hrr25p-mediated phosphorylation. J Biol Chem. 2008;283:9681-91 pubmed publisher
  4. Schäfer T, Strauss D, Petfalski E, Tollervey D, Hurt E. The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes. EMBO J. 2003;22:1370-80 pubmed
    ..Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis. ..
  5. Kafadar K, Zhu H, Snyder M, Cyert M. Negative regulation of calcineurin signaling by Hrr25p, a yeast homolog of casein kinase I. Genes Dev. 2003;17:2698-708 pubmed
    ..These findings represent the first identification of a negative regulator for Crz1p, and establish a novel physiological role for Hrr25p in antagonizing calcineurin signaling. ..
  6. Phatnani H, Jones J, Greenleaf A. Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome. Biochemistry. 2004;43:15702-19 pubmed
    ..the phosphoCTD-interacting domains of a number of these PCAPs, and in several test cases (namely, Set2, Ssd1, and Hrr25) adduced evidence that phosphoCTD binding is functionally important in vivo...
  7. Schäfer T, Maco B, Petfalski E, Tollervey D, Bottcher B, Aebi U, et al. Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit. Nature. 2006;441:651-5 pubmed
    ..Here we identify a maturation step of the yeast pre-40S subunit that is regulated by the protein kinase Hrr25 and involves ribosomal protein Rps3...
  8. Mehlgarten C, Schaffrath R. Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactiszymocin in budding yeast. Mol Genet Genomics. 2003;269:188-96 pubmed
    ..In studying its action, a resistant mutant ( kti14-1) was found to express the tot-phenotype typical of totDelta cells, toxin target (TOT) mutants that are impaired ..
  9. Lusk C, Waller D, Makhnevych T, Dienemann A, Whiteway M, Thomas D, et al. Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p. Traffic. 2007;8:647-60 pubmed
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is linked to a function for Nup53p in the spindle assembly checkpoint. ..
  10. DeMaggio A, Lindberg R, Hunter T, Hoekstra M. The budding yeast HRR25 gene product is a casein kinase I isoform. Proc Natl Acad Sci U S A. 1992;89:7008-12 pubmed
    The Saccharomyces cerevisiae HRR25 gene was identified as a regulator of DNA strand-break repair. HRR25 encodes a protein kinase that is closely related to bovine casein kinase I (CKI). CKI is a ubiquitous multipotential protein kinase...
  11. Lin Z, Dong M, Zhang Y, Lee E, Lin H. Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification. Nat Chem Biol. 2016;12:995-997 pubmed publisher
    ..The NADH- and Cbr1-dependent reduction of Dph3 may provide a regulatory linkage between cellular metabolic state and protein translation. ..
  12. Ye Q, Ur S, Su T, Corbett K. Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator. EMBO J. 2016;35:2139-2151 pubmed
    ..The CK1? kinase Hrr25 is critical for sister kinetochore co-orientation, but its roles are not well understood...
  13. Mitterer V, Murat G, Réty S, Blaud M, Delbos L, Stanborough T, et al. Sequential domain assembly of ribosomal protein S3 drives 40S subunit maturation. Nat Commun. 2016;7:10336 pubmed publisher
    ..Such a stepwise assembly may represent a new paradigm for the incorporation of ribosomal proteins. ..
  14. Faza M, Chang Y, Occhipinti L, Kemmler S, Panse V. Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes. PLoS Genet. 2012;8:e1002915 pubmed publisher
    ..Mex67-Mtr2 could engage a regulatory crosstalk among the three major export pathways for optimal cellular growth and proliferation. ..
  15. Bhandari D, Zhang J, Menon S, Lord C, Chen S, Helm J, et al. Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits. Mol Biol Cell. 2013;24:2727-38 pubmed publisher
    ..In vitro, Sit4p dephosphorylates COPII coat subunits. Consistent with a role in coat recycling, Sit4p and its mammalian orthologue, PP6, regulate traffic from the ER to the Golgi complex. ..
  16. Wang J, Davis S, Menon S, Zhang J, Ding J, Cervantes S, et al. Ypt1/Rab1 regulates Hrr25/CK1δ kinase activity in ER-Golgi traffic and macroautophagy. J Cell Biol. 2015;210:273-85 pubmed publisher
    ..Phosphorylation of the COPII coat by casein kinase 1 (CK1), Hrr25, contributes to the directional delivery of ER-derived vesicles to the Golgi...
  17. Oeljeklaus S, Reinartz B, Wolf J, Wiese S, Tonillo J, Podwojski K, et al. Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res. 2012;11:2567-80 pubmed publisher
  18. Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher
    ..In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability. ..
  19. Treusch S, Lindquist S. An intrinsically disordered yeast prion arrests the cell cycle by sequestering a spindle pole body component. J Cell Biol. 2012;197:369-79 pubmed publisher
    ..Our work illustrates how the promiscuous interactions of an intrinsically disordered protein can produce highly specific cellular toxicities through illicit, yet highly specific, interactions with the proteome. ..
  20. Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res. 2010;38:3068-80 pubmed publisher
    ..The data presented strengthen the idea that assembly of eukaryotic pre-ribosomal particles can result from transient association of distinct building blocks. ..
  21. Vancura A, Sessler A, Leichus B, Kuret J. A prenylation motif is required for plasma membrane localization and biochemical function of casein kinase I in budding yeast. J Biol Chem. 1994;269:19271-8 pubmed
    The subcellular distribution of three casein kinase I (CK1) homologs, encoded by the YCK1, YCK2, and HRR25 genes, has been determined in budding yeast through a combination of subcellular fractionation and immunofluorescence methods...
  22. Collins M, Black J, Liu Z. Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..Here, we show that casein kinase I isoform Hrr25 is an important negative regulator of Haa1. Haa1 is known to be multiply phosphorylated...
  23. Hoekstra M, Liskay R, Ou A, DeMaggio A, Burbee D, Heffron F. HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA. Science. 1991;253:1031-4 pubmed
    ..The protein HRR25 from the budding yeast Saccharomyces cerevisiae was defined by the mutation hrr25-1...
  24. Abdel Fattah W, Jablonowski D, Di Santo R, Thüring K, Scheidt V, Hammermeister A, et al. Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast. PLoS Genet. 2015;11:e1004931 pubmed publisher
    ..Elongator's largest subunit (Elp1; also known as Iki3) was phosphorylated and implicated the yeast casein kinase I Hrr25 in Elongator function...
  25. Peng Y, Grassart A, Lu R, Wong C, Yates J, Barnes G, et al. Casein kinase 1 promotes initiation of clathrin-mediated endocytosis. Dev Cell. 2015;32:231-40 pubmed publisher
    ..Here, the casein kinase 1 (CK1) Hrr25 is shown to be an endocytic protein and to be among the earliest proteins to appear at endocytic sites...
  26. Hoekstra M, Dhillon N, Carmel G, DeMaggio A, Lindberg R, Hunter T, et al. Budding and fission yeast casein kinase I isoforms have dual-specificity protein kinase activity. Mol Biol Cell. 1994;5:877-86 pubmed
    ..8-to 3.1-fold increase in the Km for poly-E4Y1 and casein. These data demonstrate that four different CKI isoforms from two different yeasts are capable of protein-tyrosine kinase activity and encode dual-specificity protein kinases. ..
  27. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  28. Zhang B, Shi Q, Varia S, Xing S, Klett B, Cook L, et al. The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies. Genetics. 2016;203:1191-202 pubmed publisher
    ..Here, we show that the Hrr25/CK1? protein kinase is recruited to cytoplasmic processing bodies (P-bodies) in an evolutionarily conserved manner...
  29. Davis S, Wang J, Zhu M, Stahmer K, Lakshminarayan R, Ghassemian M, et al. Sec24 phosphorylation regulates autophagosome abundance during nutrient deprivation. elife. 2016;5: pubmed publisher
    ..We also show casein kinase 1 (Hrr25) is a key kinase that phosphorylates this regulatory surface...
  30. Chasman D, Ho Y, Berry D, Nemec C, Macgilvray M, Hose J, et al. Pathway connectivity and signaling coordination in the yeast stress-activated signaling network. Mol Syst Biol. 2014;10:759 pubmed publisher
    ..We find that the orthologous human network is enriched for cancer-causing genes, underscoring the importance of the subnetwork's predictions in understanding stress biology. ..
  31. Nakatogawa H. Hrr25: an emerging major player in selective autophagy regulation in Saccharomyces cerevisiae. Autophagy. 2015;11:432-3 pubmed publisher
    ..Recently, we found that Hrr25, a homolog of CSNK1D/casein kinase 1δ, regulates 3 of 4 selective autophagy-related pathways in the budding ..
  32. Mochida K, Ohsumi Y, Nakatogawa H. Hrr25 phosphorylates the autophagic receptor Atg34 to promote vacuolar transport of α-mannosidase under nitrogen starvation conditions. FEBS Lett. 2014;588:3862-9 pubmed publisher
    ..In this study, we identified Hrr25 as the kinase that phosphorylates Atg34 under these conditions...
  33. Clokie S, Falconer H, Mackie S, Dubois T, Aitken A. The interaction between casein kinase Ialpha and 14-3-3 is phosphorylation dependent. FEBS J. 2009;276:6971-84 pubmed publisher
    ..We also show that both fission and budding yeast CKI kinase homologues phosphorylate mammalian and budding yeast (BMH1 and BMH2) 14-3-3 at the equivalent site. ..
  34. Pal G, Paraz M, Kellogg D. Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1. J Cell Biol. 2008;180:931-45 pubmed publisher
    ..Because casein kinase 1 is associated with sites of polar growth, it may regulate Mih1 as part of a signaling mechanism that links successful completion of growth-related events to cell cycle progression. ..
  35. Ghalei H, Schaub F, Doherty J, Noguchi Y, Roush W, Cleveland J, et al. Hrr25/CK1δ-directed release of Ltv1 from pre-40S ribosomes is necessary for ribosome assembly and cell growth. J Cell Biol. 2015;208:745-59 pubmed publisher
    Casein kinase 1δ/ε (CK1δ/ε) and their yeast homologue Hrr25 are essential for cell growth...