Gene Symbol: HPC2
Species: Saccharomyces cerevisiae S288c
Green E, Antczak A, Bailey A, Franco A, Wu K, Yates J, et al
. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005;15:2044-9 pubmed
..Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 comprise the HIR complex, which copurifies with the histone deposition protein Asf1...
Xu H, Kim U, Schuster T, Grunstein M. Identification of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:5249-59 pubmed
..Using this approach, we have isolated five mutants (hpc1, hpc2, hpc3, hpc4, and hpc5) in which cell cycle regulation of histone gene transcription is altered...
Prochasson P, Florens L, Swanson S, Washburn M, Workman J. The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev. 2005;19:2534-9 pubmed
..Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex...
Fillingham J, Kainth P, Lambert J, van Bakel H, Tsui K, Pena Castillo L, et al
. Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. Mol Cell. 2009;35:340-51 pubmed publisher
..Our data suggest that this pathway may represent a chromatin regulatory mechanism that is broadly used across the genome. ..
Formosa T, Ruone S, Adams M, Olsen A, Eriksson P, Yu Y, et al
. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Genetics. 2002;162:1557-71 pubmed
..when combined with a mutation in any member of the set of functionally related genes HIR1, HIR2/SPT1, HIR3/HPC1, or HPC2. Mutations in POB3, but not in NHP6A/B, also display strong synthetic defects with hir/hpc mutations...
Wohlschlegel J, Johnson E, Reed S, Yates J. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:45662-8 pubmed
..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes. ..
Nourani A, Robert F, Winston F. Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1496-509 pubmed
..Taken together, our results suggest a direct link for Spt2 with transcription elongation, chromatin dynamics, and genome stability. ..
Vishnoi N, Flaherty K, Hancock L, Ferreira M, Amin A, Prochasson P. Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes. Biochim Biophys Acta. 2011;1809:557-66 pubmed publisher
The HIR complex, which is comprised of the four proteins Hir1, Hir2, Hir3 and Hpc2, was first characterized as a repressor of three of the four histone gene loci in Saccharomyces cerevisiae...
Denison C, Rudner A, Gerber S, Bakalarski C, Moazed D, Gygi S. A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics. 2005;4:246-54 pubmed
..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes. ..