Gene Symbol: HMT1
Description: protein-arginine omega-N methyltransferase HMT1
Alias: HCP1, ODP1, RMT1, protein-arginine omega-N methyltransferase HMT1
Species: Saccharomyces cerevisiae S288c
Products:     HMT1

Top Publications

  1. Chen Y, Milliman E, Goulet I, Cote J, Jackson C, Vollbracht J, et al. Protein arginine methylation facilitates cotranscriptional recruitment of pre-mRNA splicing factors. Mol Cell Biol. 2010;30:5245-56 pubmed publisher
    ..Our data show that Hmt1, the major type I arginine methyltransferase, methylates Snp1, a U1 small nuclear RNP (snRNP)-specific protein, and ..
  2. Gary J, Lin W, Yang M, Herschman H, Clarke S. The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae. J Biol Chem. 1996;271:12585-94 pubmed
    ..1.1.23) in proteins of the yeast Saccharomyces cerevisiae. The RMT1 (protein-arginine methyltransferase), formerly ODP1, gene product encodes a 348-residue polypeptide of 39...
  3. Yu M, Lamming D, Eskin J, Sinclair D, Silver P. The role of protein arginine methylation in the formation of silent chromatin. Genes Dev. 2006;20:3249-54 pubmed
    ..Here we demonstrate a role for the protein arginine methyltransferase Hmt1 in this process...
  4. Yun C, Fu X. Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae. J Cell Biol. 2000;150:707-18 pubmed
  5. Weiss V, McBride A, Soriano M, Filman D, Silver P, Hogle J. The structure and oligomerization of the yeast arginine methyltransferase, Hmt1. Nat Struct Biol. 2000;7:1165-71 pubmed
    ..Hmt1/Rmt1, the major arginine methyltransferase in yeast, catalyzes methylation of arginine residues in several mRNA-binding ..
  6. Wong C, Tang H, Kong K, Wong G, Qiu H, Jin D, et al. Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. Nucleic Acids Res. 2010;38:2217-28 pubmed publisher
    ..Consistent with this, hmt1 mutants displayed increased termination at the defective gal10-Delta56 terminator...
  7. McBride A, Weiss V, Kim H, Hogle J, Silver P. Analysis of the yeast arginine methyltransferase Hmt1p/Rmt1p and its in vivo function. Cofactor binding and substrate interactions. J Biol Chem. 2000;275:3128-36 pubmed
    ..However, cells missing HMT1 and also bearing mutations in the mRNA-binding proteins Npl3p or Cbp80p can no longer survive, providing genetic ..
  8. Xu C, Henry P, Setya A, Henry M. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 2003;9:746-59 pubmed
    ..We first developed an in vivo assay to identify proteins methylated by the Hmt1 arginine methyltransferase...
  9. Lacoste N, Utley R, Hunter J, Poirier G, Cote J. Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem. 2002;277:30421-4 pubmed
    ..When compared with Rmt1, another putative yeast HMT, Dot1 shows very distinct substrate specificity...

More Information


  1. Henry M, Silver P. A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Mol Cell Biol. 1996;16:3668-78 pubmed
    ..Cloning of the cognate gene revealed an encoded protein with similarity to methyltransferases that was termed HMT1 for hnRNP methyltransferase. HMT1 is not required for normal cell viability except when NPL3 is also defective...
  2. Green D, Marfatia K, Crafton E, Zhang X, Cheng X, Corbett A. Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem. 2002;277:7752-60 pubmed
    ..Overall, these experiments provide evidence that Nab2p is an hnRNP protein that is required for poly(A) RNA export and whose export from the nucleus is regulated by Hmt1p...
  3. Shen E, Henry M, Weiss V, Valentini S, Silver P, Lee M. Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 1998;12:679-91 pubmed
    ..Together, these findings establish that one biological role for arginine methylation is in facilitating the export of certain hnRNPs out of the nucleus. ..
  4. Kuo M, Xu X, Bolck H, Guo D. Functional connection between histone acetyltransferase Gcn5p and methyltransferase Hmt1p. Biochim Biophys Acta. 2009;1789:395-402 pubmed publisher
    ..Together, these results reveal a functional connection between histone acetylation, methylation, and two of the responsible enzymes, Gcn5p and Hmt1p. ..
  5. Low J, Im H, Erce M, Hart Smith G, Snyder M, Wilkins M. Protein substrates of the arginine methyltransferase Hmt1 identified by proteome arrays. Proteomics. 2016;16:465-76 pubmed publisher
    ..By treating the arrays with recombinant arginine methyltransferase Hmt1, 42 proteins were found to be possible substrates of this enzyme...
  6. Schiza V, Molina Serrano D, Kyriakou D, Hadjiantoniou A, Kirmizis A. N-alpha-terminal acetylation of histone H4 regulates arginine methylation and ribosomal DNA silencing. PLoS Genet. 2013;9:e1003805 pubmed publisher
    ..Consistent with this, H4 N-terminal acetylation impairs the activity of the Hmt1 methyltransferase towards H4R3 in vitro...
  7. Sayegh J, Clarke S. Hsl7 is a substrate-specific type II protein arginine methyltransferase in yeast. Biochem Biophys Res Commun. 2008;372:811-5 pubmed publisher
    ..This enzyme thus may only recognize only a small subset of potential substrate proteins in yeast, in contrast to the situation with Rmt1, the major type I methyltransferase.
  8. Milliman E, Hu Z, Yu M. Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions. BMC Genomics. 2012;13:728 pubmed publisher
    ..One of the most conserved type I PRMTs is PRMT1, the homolog of which is Hmt1 in Saccharomyces cerevisiae...
  9. Kerr S, Azzouz N, Fuchs S, Collart M, Strahl B, Corbett A, et al. The Ccr4-Not complex interacts with the mRNA export machinery. PLoS ONE. 2011;6:e18302 pubmed publisher
    ..Here we demonstrate using co-immunoprecipitation approaches that Ccr4-Not subunits interact with Hmt1, the budding yeast ortholog of PRMT1...
  10. Yagoub D, Hart Smith G, Moecking J, Erce M, Wilkins M. Yeast proteins Gar1p, Nop1p, Npl3p, Nsr1p, and Rps2p are natively methylated and are substrates of the arginine methyltransferase Hmt1p. Proteomics. 2015;15:3209-18 pubmed publisher
    The Hmt1 methyltransferase is the predominant arginine methyltransferase in Saccharomyces cerevisiae...
  11. Li H, Gong T, Zhou Z, Liu Y, Cao X, He Y, et al. Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro. Biochem J. 2015;467:507-15 pubmed publisher
    ..PRMTs' substrates include histones and a variety of non-histone proteins. Previous studies have shown that yeast Hmt1 is a type I PRMT and methylates histone H4 arginine 3 and several mRNA-binding proteins...
  12. Lien P, Izumikawa K, Muroi K, Irie K, Suda Y, Irie K. Analysis of the Physiological Activities of Scd6 through Its Interaction with Hmt1. PLoS ONE. 2016;11:e0164773 pubmed publisher
    ..Herein, we report that Scd6 is a substrate of the intrinsic protein arginine methyltransferase, Hmt1, in budding yeast Saccharomyces cerevisiae...
  13. Lipson R, Webb K, Clarke S. Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2010;391:1658-62 pubmed publisher
    ..We find that this modification of Rps2 is dependent upon the major arginine methyltransferase 1 (Rmt1) in S. cerevisiae...
  14. Duttler S, Pechmann S, Frydman J. Principles of cotranslational ubiquitination and quality control at the ribosome. Mol Cell. 2013;50:379-93 pubmed publisher
    ..We find that quality control at the ribosome is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becoming accessible to ubiquitination. ..
  15. Hsieh C, Huang S, Wu Y, Liu L, Han C, Liu Y, et al. Expression of proteins with dimethylarginines in Escherichia coli for protein-protein interaction studies. Protein Sci. 2007;16:919-28 pubmed
    ..A yeast type I arginine methyltransferase gene (HMT1) is put on a plasmid under the control of the Escherichia coli methionine aminopeptidase promoter for constitutive ..
  16. Hsieh C, Tam M. Detection of dimethylarginines in protein hydrolysates by matrix-assisted laser desorption/ionization mass spectrometry. Anal Biochem. 2006;350:151-5 pubmed
    ..MALDI-TOF analysis of the acid hydrolysates confirmed the presence of dimethylarginines. The detection limit of the method is estimated at approximately 1pmol of protein. ..
  17. Inoue K, Mizuno T, Wada K, Hagiwara M. Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p. J Biol Chem. 2000;275:32793-9 pubmed
    ..Thus, Air1p and Air2p may affect mRNA transport by regulating the arginine methylation state of heterogeneous nuclear ribonucleoproteins...
  18. Klein S, Carroll J, Chen Y, Henry M, Henry P, Ortonowski I, et al. Biochemical analysis of the arginine methylation of high molecular weight fibroblast growth factor-2. J Biol Chem. 2000;275:3150-7 pubmed
    ..These findings indicate that HMW FGF-2, with the presence of five or more dimethylated Gly-Arg-Gly repeats, contains an RGG box-like domain, which may be important for protein-protein and/or protein-RNA interactions. ..
  19. Hart Smith G, Low J, Erce M, Wilkins M. Enhanced methylarginine characterization by post-translational modification-specific targeted data acquisition and electron-transfer dissociation mass spectrometry. J Am Soc Mass Spectrom. 2012;23:1376-89 pubmed publisher
    ..016) in average Mascot ion scores of methylarginine-carrying peptides. The present PTM-specific targeted data acquisition approach, though described using methylarginine, is applicable to any ionizable PTM of known mass. ..
  20. Balzer R, Henry M. Snu56p is required for Mer1p-activated meiotic splicing. Mol Cell Biol. 2008;28:2497-508 pubmed publisher
    ..Interestingly, these two proteins do not interact, suggesting that Snu56p links pre-mRNA-bound Mer1p to Nam8p in the U1 snRNP. This work demonstrates that the Snu56 protein is required for splicing only during meiosis. ..