HIR2

Summary

Gene Symbol: HIR2
Description: Hir2p
Alias: SPT1, Hir2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Spector M, Raff A, deSilva H, Lee K, Osley M. Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle. Mol Cell Biol. 1997;17:545-52 pubmed
    ..When Hir1p and Hir2p were artificially tethered to yeast promoters, each protein repressed transcription, suggesting that they ..
  2. Fillingham J, Kainth P, Lambert J, van Bakel H, Tsui K, Pena Castillo L, et al. Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. Mol Cell. 2009;35:340-51 pubmed publisher
    ..Our data suggest that this pathway may represent a chromatin regulatory mechanism that is broadly used across the genome. ..
  3. Biswas D, Takahata S, Xin H, Dutta Biswas R, Yu Y, Formosa T, et al. A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae. Genetics. 2008;178:649-59 pubmed publisher
    ..The pob3 defect in S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication. ..
  4. Green E, Antczak A, Bailey A, Franco A, Wu K, Yates J, et al. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005;15:2044-9 pubmed
    ..Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 comprise the HIR complex, which copurifies with the histone deposition protein Asf1...
  5. Prochasson P, Florens L, Swanson S, Washburn M, Workman J. The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev. 2005;19:2534-9 pubmed
    ..Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex...
  6. Dimova D, Nackerdien Z, Furgeson S, Eguchi S, Osley M. A role for transcriptional repressors in targeting the yeast Swi/Snf complex. Mol Cell. 1999;4:75-83 pubmed
    ..show that Swi/Snf is required for expression of the yeast histone HTA1-HTB1 locus because of the role of Hir1p and Hir2p corepressors in negatively regulating transcription...
  7. deSilva H, Lee K, Osley M. Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics. 1998;148:657-67 pubmed
    ..Although Hir1p and Hir2p are physically associated in yeast, Hir1p is able to function independently of Hir2p when it is artificially ..
  8. Gunjan A, Verreault A. A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae. Cell. 2003;115:537-49 pubmed
    ..Our results argue that Rad53 contributes to genome stability independently of Mec1 by preventing the damaging effects of excess histones both during normal cell cycle progression and in response to DNA damage. ..
  9. Harkness T, Arnason T, Legrand C, Pisclevich M, Davies G, Turner E. Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:673-84 pubmed
    ..Furthermore, increased expression of genes encoding Asf1p, Hir1p, and Hir2p suppressed the apc5(CA) Ts- defect in a CAF-I-dependent manner...

More Information

Publications19

  1. Nourani A, Robert F, Winston F. Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1496-509 pubmed
    ..Taken together, our results suggest a direct link for Spt2 with transcription elongation, chromatin dynamics, and genome stability. ..
  2. Ferreira M, Flaherty K, Prochasson P. The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes. PLoS ONE. 2011;6:e21113 pubmed publisher
  3. Amin A, Dimova D, Ferreira M, Vishnoi N, Hancock L, Osley M, et al. The mitotic Clb cyclins are required to alleviate HIR-mediated repression of the yeast histone genes at the G1/S transition. Biochim Biophys Acta. 2012;1819:16-27 pubmed publisher
    ..The HIR complex, comprised of Hir1, Hir2, Hir3 and Hpc2, regulates three of the four histone gene loci...
  4. Stevens J, O Donnell A, Perry T, Benjamin J, Barnes C, Johnston G, et al. FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation. PLoS ONE. 2011;6:e25644 pubmed publisher
  5. Sharp J, Fouts E, Krawitz D, Kaufman P. Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol. 2001;11:463-73 pubmed
    ..Also, Asf1p bound to the Hir1p and Hir2p proteins in vitro and in cell extracts...
  6. Kaufman P, Cohen J, Osley M. Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I. Mol Cell Biol. 1998;18:4793-806 pubmed
  7. Gradolatto A, Rogers R, Lavender H, Taverna S, Allis C, Aitchison J, et al. Saccharomyces cerevisiae Yta7 regulates histone gene expression. Genetics. 2008;179:291-304 pubmed publisher
    ..Our work suggests a mechanism in which Yta7 is localized to chromatin to establish regions of transcriptional silencing, and that one facet of this cellular mechanism is to modulate transcription of histone genes. ..
  8. Lin L, Minard L, Johnston G, Singer R, Schultz M. Asf1 can promote trimethylation of H3 K36 by Set2. Mol Cell Biol. 2010;30:1116-29 pubmed publisher
    ..This function of Asf1 promotes the switch from di- to trimethylation of H3 K36 at that gene. These results support the view that Set2 function in chromatin metabolism can intimately involve histone chaperone Asf1. ..
  9. Sherwood P, Tsang S, Osley M. Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:28-38 pubmed
    The products of the HIR1 and HIR2 genes have been defined genetically as repressors of histone gene transcription in S. cerevisiae...
  10. Vishnoi N, Flaherty K, Hancock L, Ferreira M, Amin A, Prochasson P. Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes. Biochim Biophys Acta. 2011;1809:557-66 pubmed publisher
    The HIR complex, which is comprised of the four proteins Hir1, Hir2, Hir3 and Hpc2, was first characterized as a repressor of three of the four histone gene loci in Saccharomyces cerevisiae...