Gene Symbol: HHT2
Description: histone H3
Alias: histone H3
Species: Saccharomyces cerevisiae S288c
Products:     HHT2

Top Publications

  1. Blackwell J, Wilkinson S, Mosammaparast N, Pemberton L. Mutational analysis of H3 and H4 N termini reveals distinct roles in nuclear import. J Biol Chem. 2007;282:20142-50 pubmed
    ..Acetylation may be important for modulating the interaction with transport factors and may play a role in the release of histones from karyopherins in the nucleus. ..
  2. Abshiru N, Ippersiel K, Tang Y, Yuan H, Marmorstein R, Verreault A, et al. Chaperone-mediated acetylation of histones by Rtt109 identified by quantitative proteomics. J Proteomics. 2013;81:80-90 pubmed publisher
    ..Rtt109 is a fungal-specific histone acetyltransferase (HAT) that associates with either Vps75 or Asf1 to acetylate histone H3. Recent biochemical and structural studies suggest that site-specific acetylation of H3 by Rtt109 is dictated by ..
  3. Adkins M, Carson J, English C, Ramey C, Tyler J. The histone chaperone anti-silencing function 1 stimulates the acetylation of newly synthesized histone H3 in S-phase. J Biol Chem. 2007;282:1334-40 pubmed
    ..We have found that budding yeast lacking Asf1 has greatly reduced levels of histone H3 acetylated at lysine 9...
  4. Feser J, Truong D, Das C, Carson J, KIEFT J, Harkness T, et al. Elevated histone expression promotes life span extension. Mol Cell. 2010;39:724-35 pubmed publisher
    ..Indeed, yeast lacking the histone chaperone Asf1 or acetylation of histone H3 on lysine 56 are short lived, and this appears to be at least partly due to their having decreased histone ..
  5. van Welsem T, Frederiks F, Verzijlbergen K, Faber A, Nelson Z, Egan D, et al. Synthetic lethal screens identify gene silencing processes in yeast and implicate the acetylated amino terminus of Sir3 in recognition of the nucleosome core. Mol Cell Biol. 2008;28:3861-72 pubmed publisher
    Dot1 methylates histone H3 lysine 79 (H3K79) on the nucleosome core and is involved in Sir protein-mediated silencing...
  6. Poveda A, Sendra R. Site specificity of yeast histone acetyltransferase B complex in vivo. FEBS J. 2008;275:2122-36 pubmed publisher
    ..Strikingly, histone H3 was not found in any of the immunoprecipitates obtained with the different components of the HAT-B enzyme, ..
  7. Driscoll R, Hudson A, Jackson S. Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56. Science. 2007;315:649-52 pubmed
    ..Furthermore, we show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo...
  8. Reis C, Campbell J. Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae. Genetics. 2007;175:993-1010 pubmed
    ..These results identify TRF4, TRF5, and RRP6 as new players in the regulation of histone mRNA levels in yeast. To our knowledge, the histone transcripts are the first mRNAs that are upregulated in Trf mutants. ..
  9. Camahort R, Shivaraju M, Mattingly M, Li B, Nakanishi S, Zhu D, et al. Cse4 is part of an octameric nucleosome in budding yeast. Mol Cell. 2009;35:794-805 pubmed publisher
    ..Taken together, our experimental evidence supports the model that the Cse4 nucleosome is an octamer, containing two copies each of Cse4, H2A, H2B, and H4. ..

More Information


  1. Tatum D, Li S. Evidence that the histone methyltransferase Dot1 mediates global genomic repair by methylating histone H3 on lysine 79. J Biol Chem. 2011;286:17530-5 pubmed publisher
    ..Dot1, a histone methyltransferase required for methylation of histone H3 lysine 79 (H3K79), has been shown to confer yeast cells with resistance to DNA-damaging agents and play a role ..
  2. English C, Maluf N, Tripet B, Churchill M, Tyler J. ASF1 binds to a heterodimer of histones H3 and H4: a two-step mechanism for the assembly of the H3-H4 heterotetramer on DNA. Biochemistry. 2005;44:13673-82 pubmed
    The first step in the formation of the nucleosome is commonly assumed to be the deposition of a histone H3-H4 heterotetramer onto DNA...
  3. Mosammaparast N, Guo Y, Shabanowitz J, Hunt D, Pemberton L. Pathways mediating the nuclear import of histones H3 and H4 in yeast. J Biol Chem. 2002;277:862-8 pubmed
    ..H3 and H4 are the first histones to be assembled onto DNA, and these results show that their import is mediated by at least two import pathways. ..
  4. Singh R, Kabbaj M, Paik J, Gunjan A. Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Nat Cell Biol. 2009;11:925-33 pubmed publisher
    ..We have identified the Tyr 99 residue of histone H3 as being critical for the efficient ubiquitylation and degradation of this histone...
  5. Morillo Huesca M, Maya D, Muñoz Centeno M, Singh R, Oreal V, Reddy G, et al. FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1. PLoS Genet. 2010;6:e1000964 pubmed publisher
  6. Duina A, Rufiange A, Bracey J, Hall J, Nourani A, Winston F. Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in Saccharomyces cerevisiae. Genetics. 2007;177:101-12 pubmed
    A previous study of histone H3 in Saccharomyces cerevisiae identified a mutant with a single amino acid change, leucine 61 to tryptophan, that confers several transcriptional defects...
  7. Han J, Zhou H, Li Z, Xu R, Zhang Z. The Rtt109-Vps75 histone acetyltransferase complex acetylates non-nucleosomal histone H3. J Biol Chem. 2007;282:14158-64 pubmed
    Acetylation of lysine 56 of histone H3 (H3-Lys-56) occurs in S phase and disappears during G(2)/M phase of the cell cycle. However, it is not clear how this modification is regulated during the progression of the cell cycle...
  8. Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T. Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4. Nature. 2007;446:338-41 pubmed
    ..7 A resolution, of CIA-I in complex with histones H3 and H4. The structure shows the histone H3-H4 dimer's mutually exclusive interactions with another histone H3-H4 dimer and CIA-I...
  9. Altaf M, Utley R, Lacoste N, Tan S, Briggs S, Cote J. Interplay of chromatin modifiers on a short basic patch of histone H4 tail defines the boundary of telomeric heterochromatin. Mol Cell. 2007;28:1002-14 pubmed
    Dot1 (Disruptor of telomeric silencing-1) is a histone H3 lysine 79 methyltransferase that contributes to the establishment of heterochromatin boundary and has been linked to transcription elongation...
  10. Kawashima S, Nakabayashi Y, Matsubara K, Sano N, Enomoto T, Tanaka K, et al. Global analysis of core histones reveals nucleosomal surfaces required for chromosome bi-orientation. EMBO J. 2011;30:3353-67 pubmed publisher
    ..Kinetochore assembly requires centromere-specific nucleosomes containing the histone H3 variant CenH3...
  11. van Leeuwen F, Gafken P, Gottschling D. Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell. 2002;109:745-56 pubmed
    ..cerevisiae. We now find that Dot1p methylates histone H3 on lysine 79, which maps to the top and bottom of the nucleosome core...
  12. Lin C, Yuan Y. Structural insights into histone H3 lysine 56 acetylation by Rtt109. Structure. 2008;16:1503-10 pubmed publisher
    ..of histone acetyltransferases (HATs), which promote genome stability by directly acetylating newly synthesized histone H3 lysine 56 (H3-K56) through an unknown mechanism. Here, we report the crystal structures of Rtt109 at 2...
  13. Norris A, Bianchet M, Boeke J. Compensatory interactions between Sir3p and the nucleosomal LRS surface imply their direct interaction. PLoS Genet. 2008;4:e1000301 pubmed publisher
    ..Conversely, suppressors of LRS alleles in either SIR3, histone H3, or H4 also tend to make their respective surfaces less electronegative...
  14. Grant P, Eberharter A, John S, Cook R, Turner B, Workman J. Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem. 1999;274:5895-900 pubmed
    ..histones is significantly reduced relative to its activity on free histones, where it predominantly modifies histone H3 at lysine 14...
  15. Ng H, Feng Q, Wang H, Erdjument Bromage H, Tempst P, Zhang Y, et al. Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association. Genes Dev. 2002;16:1518-27 pubmed
    ..Here, we report that lysine (Lys) 79 of histone H3, which resides in the globular domain, is methylated in eukaryotic organisms...
  16. Agez M, Chen J, Guerois R, van Heijenoort C, Thuret J, Mann C, et al. Structure of the histone chaperone ASF1 bound to the histone H3 C-terminal helix and functional insights. Structure. 2007;15:191-9 pubmed
    Asf1 is a histone chaperone that favors histone H3/H4 assembly and disassembly...
  17. Katan Khaykovich Y, Struhl K. Splitting of H3-H4 tetramers at transcriptionally active genes undergoing dynamic histone exchange. Proc Natl Acad Sci U S A. 2011;108:1296-301 pubmed publisher
    ..In contrast, tetramer splitting, dimer exchange, and nucleosomes with mixed H3-H4 tetramers occur at highly active genes, presumably linked to rapid histone exchange associated with robust transcription. ..
  18. Suter B, Pogoutse O, Guo X, Krogan N, Lewis P, Greenblatt J, et al. Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2p. BMC Biol. 2007;5:38 pubmed
    ..Additional genetic and biochemical evidence points to the existence of partly overlapping histone H3 acetyltransferase activities in addition to Hat1p/Hat2p for proper DNA replication efficiency...
  19. Kim H, Seol J, Han J, Youn H, Cho E. Histone chaperones regulate histone exchange during transcription. EMBO J. 2007;26:4467-74 pubmed
    ..This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone ..
  20. Verzijlbergen K, van Welsem T, Sie D, Lenstra T, Turner D, Holstege F, et al. A barcode screen for epigenetic regulators reveals a role for the NuB4/HAT-B histone acetyltransferase complex in histone turnover. PLoS Genet. 2011;7:e1002284 pubmed publisher
    ..Thus, systematic and direct interrogation of chromatin structure on DNA barcodes can lead to the discovery of genes and pathways involved in chromatin modification and dynamics. ..
  21. Evans M, Bostelman L, Albrecht A, Keller A, Strande N, Thompson J. UV sensitive mutations in histone H3 in Saccharomyces cerevisiae that alter specific K79 methylation states genetically act through distinct DNA repair pathways. Curr Genet. 2008;53:259-74 pubmed publisher
    ..We have previously shown that histone H3 K79 methylation is important for repair of UV-induced DNA damage in Saccharomyces cerevisiae, acting through ..
  22. Hyland E, Cosgrove M, Molina H, Wang D, Pandey A, Cottee R, et al. Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:10060-70 pubmed
    ..Furthermore, we provide direct mass spectrometry evidence for the existence of histone H3 K56 acetylation in yeast...
  23. Gunjan A, Verreault A. A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae. Cell. 2003;115:537-49 pubmed
    ..Our results argue that Rad53 contributes to genome stability independently of Mec1 by preventing the damaging effects of excess histones both during normal cell cycle progression and in response to DNA damage. ..
  24. Lacoste N, Utley R, Hunter J, Poirier G, Cote J. Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem. 2002;277:30421-4 pubmed
    ..While Rmt1 methylates histone H4, Dot1 targets histone H3. In contrast to Rmt1, which can only modify free histones, Dot1 activity is specific to nucleosomal substrates...
  25. Park Y, Sudhoff K, Andrews A, Stargell L, Luger K. Histone chaperone specificity in Rtt109 activation. Nat Struct Mol Biol. 2008;15:957-64 pubmed
    ..Nap1 and Vps75 interact with histones and Rtt109 with comparable affinities. However, only Vps75 stimulates Rtt109 enzymatic activity. Our data highlight the functional specificity of Vps75 in Rtt109 activation. ..
  26. Li Q, Zhou H, Wurtele H, Davies B, Horazdovsky B, Verreault A, et al. Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly. Cell. 2008;134:244-55 pubmed publisher
    ..Here we show that histone H3 acetylated at lysine 56 (H3K56Ac) is incorporated onto replicating DNA and, by increasing the binding affinity ..
  27. Chu Y, Sutton A, Sternglanz R, Prelich G. The BUR1 cyclin-dependent protein kinase is required for the normal pattern of histone methylation by SET2. Mol Cell Biol. 2006;26:3029-38 pubmed
    ..This selection identified mutations in SET2, which encodes a histone methylase that targets lysine 36 of histone H3 and, like BUR1, has a poorly characterized role during transcription elongation...
  28. Burgess R, Zhou H, Han J, Zhang Z. A role for Gcn5 in replication-coupled nucleosome assembly. Mol Cell. 2010;37:469-80 pubmed publisher
    ..These results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 association with CAF-1 via H3 acetylation. ..
  29. Ge Z, Wang H, Parthun M. Nuclear Hat1p complex (NuB4) components participate in DNA repair-linked chromatin reassembly. J Biol Chem. 2011;286:16790-9 pubmed publisher
    ..reassembly accompanying DNA double strand break repair, ChIP analysis can be used to monitor the presence of histone H3 near the lesion...
  30. Gradolatto A, Rogers R, Lavender H, Taverna S, Allis C, Aitchison J, et al. Saccharomyces cerevisiae Yta7 regulates histone gene expression. Genetics. 2008;179:291-304 pubmed publisher
    ..gene loci appeared to occur through binding of the bromodomain-like region of Yta7 with the N-terminal tail of histone H3. Our work suggests a mechanism in which Yta7 is localized to chromatin to establish regions of transcriptional ..
  31. Campos E, Fillingham J, Li G, Zheng H, Voigt P, Kuo W, et al. The program for processing newly synthesized histones H3.1 and H4. Nat Struct Mol Biol. 2010;17:1343-51 pubmed publisher
    ..histones are imported into the nucleus, we biochemically purified and characterized the full gamut of histone H3.1-containing complexes from human cytoplasmic fractions and identified their associated histone post-..
  32. Takahashi Y, Lee J, Swanson S, Saraf A, Florens L, Washburn M, et al. Regulation of H3K4 trimethylation via Cps40 (Spp1) of COMPASS is monoubiquitination independent: implication for a Phe/Tyr switch by the catalytic domain of Set1. Mol Cell Biol. 2009;29:3478-86 pubmed publisher
    The multiprotein complex Set1/COMPASS is the founding member of the histone H3 lysine 4 (H3K4) methyltransferases, whose human homologs include the MLL and hSet1 complexes...
  33. Sun B, Hong J, Zhang P, Dong X, Shen X, Lin D, et al. Molecular basis of the interaction of Saccharomyces cerevisiae Eaf3 chromo domain with methylated H3K36. J Biol Chem. 2008;283:36504-12 pubmed publisher
    ..Eaf3 contains a chromo domain at the N terminus that can bind to methylated Lys-36 of histone H3 (H3K36). We report here the crystal structures of the Eaf3 chromo domain in two truncation forms...
  34. Clarke A, Lowell J, Jacobson S, Pillus L. Esa1p is an essential histone acetyltransferase required for cell cycle progression. Mol Cell Biol. 1999;19:2515-26 pubmed
    ..These observations therefore link an essential HAT activity to cell cycle progression, potentially through discrete transcriptional regulatory events. ..
  35. Masumoto H, Hawke D, Kobayashi R, Verreault A. A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response. Nature. 2005;436:294-8 pubmed
    ..Here we show that lysine 56 (K56) acetylation is an abundant modification of newly synthesized histone H3 molecules that are incorporated into chromosomes during S phase...
  36. VanDemark A, Blanksma M, Ferris E, Heroux A, Hill C, Formosa T. The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol Cell. 2006;22:363-74 pubmed
    ..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. ..
  37. Lochmann B, Ivanov D. Histone H3 localizes to the centromeric DNA in budding yeast. PLoS Genet. 2012;8:e1002739 pubmed publisher
    ..as in other eukaryotes, the Cse4 histone variant (known in vertebrates as CENP-A) is believed to substitute for histone H3 at the centromeric nucleosome. However, the exact composition of the CEN nucleosome remains a subject of debate...
  38. Hong J, Feng H, Zhou Z, Ghirlando R, Bai Y. Identification of functionally conserved regions in the structure of the chaperone/CenH3/H4 complex. J Mol Biol. 2013;425:536-45 pubmed publisher
    In eukaryotes, a variant of conventional histone H3 termed CenH3 epigenetically marks the centromere. The conserved CenH3 chaperone specifically recognizes CenH3 and is required for CenH3 deposition at the centromere...
  39. Wu W, Alami S, Luk E, Wu C, Sen S, Mizuguchi G, et al. Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange. Nat Struct Mol Biol. 2005;12:1064-71 pubmed
    ..Finally, the C-terminal alpha-helix of H2AZ is crucial for its recognition by SWR1. These findings provide insight on the initial events of histone exchange. ..
  40. Fry C, Norris A, Cosgrove M, Boeke J, Peterson C. The LRS and SIN domains: two structurally equivalent but functionally distinct nucleosomal surfaces required for transcriptional silencing. Mol Cell Biol. 2006;26:9045-59 pubmed
    ..Each of these domains consists of histone H3 and H4 L1 and L2 loops that form a DNA-binding surface at either superhelical location (SHL) +/-2...
  41. Shi X, Kachirskaia I, Walter K, Kuo J, Lake A, Davrazou F, et al. Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36. J Biol Chem. 2007;282:2450-5 pubmed
    ..We provide evidence on the genomic scale that PHD fingers constitute a general class of effector modules for histone H3 trimethylated at lysine 4 (H3K4me3) and histone H3 trimethylated at lysine 36 (H3K36me3)...
  42. Tang Y, Holbert M, Delgoshaie N, Wurtele H, Guillemette B, Meeth K, et al. Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Structure. 2011;19:221-31 pubmed publisher
    Yeast Rtt109 promotes nucleosome assembly and genome stability by acetylating K9, K27, and K56 of histone H3 through interaction with either of two distinct histone chaperones, Vps75 or Asf1...
  43. Yu Y, Srinivasan M, Nakanishi S, Leatherwood J, Shilatifard A, Sternglanz R. A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast. Mol Cell Biol. 2011;31:2311-25 pubmed publisher
  44. Lee J, Shukla A, Schneider J, Swanson S, Washburn M, Florens L, et al. Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS. Cell. 2007;131:1084-96 pubmed
    COMPASS, the yeast homolog of the mammalian MLL complex, is a histone H3 lysine 4 (H3K4) methylase consisting of Set1 (KMT2) and seven other polypeptides, including Cps35, the only essential subunit...
  45. Sommermeyer V, Béneut C, Chaplais E, Serrentino M, Borde V. Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes. Mol Cell. 2013;49:43-54 pubmed publisher
    ..This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination. ..
  46. Duina A, Winston F. Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin. Mol Cell Biol. 2004;24:561-72 pubmed
    We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects...
  47. Onishi M, Liou G, Buchberger J, Walz T, Moazed D. Role of the conserved Sir3-BAH domain in nucleosome binding and silent chromatin assembly. Mol Cell. 2007;28:1015-28 pubmed
    ..its binding to nucleosomes is regulated by residues in the N terminus of histone H4 and the globular domain of histone H3 on the exposed surface of the nucleosome...
  48. Castellano Pozo M, Santos Pereira J, Rondon A, Barroso S, Andújar E, Pérez Alegre M, et al. R loops are linked to histone H3 S10 phosphorylation and chromatin condensation. Mol Cell. 2013;52:583-90 pubmed publisher
    ..Here we show that R loops are tightly linked to histone H3 S10 phosphorylation (H3S10P), a mark of chromatin condensation...
  49. Myers C, Berner G, Holthoff J, Martinez Fonts K, Harper J, Alford S, et al. Mutant versions of the S. cerevisiae transcription elongation factor Spt16 define regions of Spt16 that functionally interact with histone H3. PLoS ONE. 2011;6:e20847 pubmed publisher
    ..the middle domain of the FACT subunit Spt16--the Spt16-M domain--is involved in functional interactions with histone H3. Our results show that the Spt16-M domain plays a role in the prevention of cryptic intragenic transcription ..
  50. Stulemeijer I, De Vos D, van Harten K, Joshi O, Blomberg O, van Welsem T, et al. Dot1 histone methyltransferases share a distributive mechanism but have highly diverged catalytic properties. Sci Rep. 2015;5:9824 pubmed publisher
    ..Dot1 establishes an H3K79 methylation pattern consisting of mono-, di- and trimethylation states on histone H3 via a distributive mechanism...
  51. Pryde F, Jain D, Kerr A, Curley R, Mariotti F, Vogelauer M. H3 k36 methylation helps determine the timing of cdc45 association with replication origins. PLoS ONE. 2009;4:e5882 pubmed publisher
    ..Here we identify histone H3 K36 methylation (H3 K36me) by Set2 as a novel regulator of the time of Cdc45 association with replication ..
  52. Enomoto S, Johnston S, Berman J. Identification of a novel allele of SIR3 defective in the maintenance, but not the establishment, of silencing in Saccharomyces cerevisiae. Genetics. 2000;155:523-38 pubmed
    ..Furthermore, HM silencing is most vulnerable to disruption by the Sir3-P898R C terminus immediately after S-phase, the time when new silent chromatin is assembled onto newly replicated DNA. ..
  53. Nair D, Ge Z, Mersfelder E, Parthun M. Genetic interactions between POB3 and the acetylation of newly synthesized histones. Curr Genet. 2011;57:271-86 pubmed publisher
    ..For histone H3, lysine residues 14 and 23 were particularly important when POB3 activity is compromised...
  54. Su X, Pillus L. Functions for diverse metabolic activities in heterochromatin. Proc Natl Acad Sci U S A. 2016;113:E1526-35 pubmed publisher
    ..Enhanced N-terminal histone H3 proteolysis is observed in GDH1 mutants, consistent with telomeric silencing defects...
  55. Jiao Y, Seeger K, Lautrette A, Gaubert A, Mousson F, Guerois R, et al. Surprising complexity of the Asf1 histone chaperone-Rad53 kinase interaction. Proc Natl Acad Sci U S A. 2012;109:2866-71 pubmed publisher
    ..We identified a rad53 mutation that destabilized the Asf1-Rad53 complex and increased the viability of rad9 and rad24 mutants in conditions of genotoxic stress, suggesting that complex stability impacts the DNA damage response. ..
  56. Ehrentraut S, Hassler M, Oppikofer M, Kueng S, Weber J, Mueller J, et al. Structural basis for the role of the Sir3 AAA+ domain in silencing: interaction with Sir4 and unmethylated histone H3K79. Genes Dev. 2011;25:1835-46 pubmed publisher
    ..In summary, the unique folding of this conserved Sir3 AAA(+) domain generates novel surface regions that mediate Sir3-Sir4 and Sir3-nucleosome interactions, both being required for the proper assembly of heterochromatin in living cells. ..
  57. Chang J, Winston F. Spt10 and Spt21 are required for transcriptional silencing in Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:118-29 pubmed publisher
    ..These results suggest that Spt10 and Spt21 control silencing in S. cerevisiae by altering chromatin structure through roles beyond the control of histone gene expression. ..
  58. Zhang W, Bone J, Edmondson D, Turner B, Roth S. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J. 1998;17:3155-67 pubmed
  59. Schibler A, Koutelou E, Tomida J, Wilson Pham M, Wang L, Lu Y, et al. Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2. Genes Dev. 2016;30:1187-97 pubmed publisher
    b>Histone H3 methylation on Lys4 (H3K4me) is associated with active gene transcription in all eukaryotes. In Saccharomyces cerevisiae, Set1 is the sole lysine methyltransferase required for mono-, di-, and trimethylation of this site...