Gene Symbol: HEM15
Description: ferrochelatase HEM15
Alias: ferrochelatase HEM15
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. He Y, Alam S, Proteasa S, Zhang Y, Lesuisse E, Dancis A, et al. Yeast frataxin solution structure, iron binding, and ferrochelatase interaction. Biochemistry. 2004;43:16254-62 pubmed
    ..NMR spectroscopy was further used to identify the intermolecular binding interface between ferrochelatase and frataxin. Ferrochelatase appears to bind to frataxin's helical plane in a manner that includes its iron-binding interface. ..
  2. Labbe Bois R. The ferrochelatase from Saccharomyces cerevisiae. Sequence, disruption, and expression of its structural gene HEM15. J Biol Chem. 1990;265:7278-83 pubmed
    The HEM15 gene in Saccharomyces cerevisiae encodes ferrochelatase (EC 4.99.1...
  3. Eldridge M, Dailey H. Yeast ferrochelatase: expression in a baculovirus system and purification of the expression protein. Protein Sci. 1992;1:271-7 pubmed
    ..To our knowledge this is the first example of a mitochondrial membrane protein that has been expressed in a baculovirus system. ..
  4. Panni S, Montecchi Palazzi L, Kiemer L, Cabibbo A, Paoluzi S, Santonico E, et al. Combining peptide recognition specificity and context information for the prediction of the 14-3-3-mediated interactome in S. cerevisiae and H. sapiens. Proteomics. 2011;11:128-43 pubmed publisher
    ..Our approach provides an orthogonal reliability assessment and maps with high confidence the 14-3-3 peptide target on the partner proteins. ..
  5. Karlberg T, Lecerof D, Gora M, Silvegren G, Labbe Bois R, Hansson M, et al. Metal binding to Saccharomyces cerevisiae ferrochelatase. Biochemistry. 2002;41:13499-506 pubmed
    ..The latter site is also located at the surface of the molecule and thought to be involved in initial metal binding and regulation. ..
  6. Khoshnevis S, Gross T, Rotte C, Baierlein C, Ficner R, Krebber H. The iron-sulphur protein RNase L inhibitor functions in translation termination. EMBO Rep. 2010;11:214-9 pubmed publisher
  7. Prasad A, Dailey H. Effect of cellular location on the function of ferrochelatase. J Biol Chem. 1995;270:18198-200 pubmed
    ..Interestingly in cells with improperly localized ferrochelatase the amount of b-type cytochrome decreased by 80% as opposed to c- and a-type cytochromes where the decreases were only 60 and 40%, respectively. ..
  8. Söderberg C, Gillam M, Ahlgren E, Hunter G, Gakh O, Isaya G, et al. The Structure of the Complex between Yeast Frataxin and Ferrochelatase: CHARACTERIZATION AND PRE-STEADY STATE REACTION OF FERROUS IRON DELIVERY AND HEME SYNTHESIS. J Biol Chem. 2016;291:11887-98 pubmed publisher
    ..Furthermore, they support the proposal that frataxin-mediated delivery of this potentially toxic substrate overcomes formation of reactive oxygen species. ..
  9. Kim H, Jeong M, Parnell T, Babst M, Phillips J, Winge D. The Plasma Membrane Protein Nce102 Implicated in Eisosome Formation Rescues a Heme Defect in Mitochondria. J Biol Chem. 2016;291:17417-26 pubmed publisher
    ..of this trafficking pathway, we created a synthetic lesion in the heme biosynthetic pathway by deleting the gene HEM15 encoding the enzyme ferrochelatase in S. cerevisiae and performed a genetic suppressor screen...

More Information


  1. Grzybowska E, Gora M, Plochocka D, Rytka J. Saccharomyces cerevisiae ferrochelatase forms a homodimer. Arch Biochem Biophys. 2002;398:170-8 pubmed
    ..We propose a structural model of yeast ferrochelatase, based on the known structure of the human enzyme, which helps us to understand the differences in dimerization between the wild-type and mutant proteins. ..
  2. Urban Grimal D, Labbe Bois R. Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Mol Gen Genet. 1981;183:85-92 pubmed
    ..These results are discussed in terms of possible modes of regulation of heme synthesis pathway in yeast. ..
  3. Lesuisse E, Santos R, Matzanke B, Knight S, Camadro J, Dancis A. Iron use for haeme synthesis is under control of the yeast frataxin homologue (Yfh1). Hum Mol Genet. 2003;12:879-89 pubmed
    ..Deltayfh1 strains, the level of ferrochelatase (Hem15p) was very low, as a result of transcriptional repression of HEM15. However, the low amount of Hem15p was not the cause of haeme deficiency in Deltayfh1 cells...
  4. Kilmartin J. Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. J Cell Biol. 2003;162:1211-21 pubmed
    ..The centrosomal human protein containing Sfi1 repeats also binds centrin in the repeat region, showing that this centrin-binding motif is conserved. ..