Gene Symbol: HEK2
Description: Hek2p
Alias: KHD1, Hek2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Paquin N, Ménade M, Poirier G, Donato D, Drouet E, Chartrand P. Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p. Mol Cell. 2007;26:795-809 pubmed
    ..We now report that the RNA binding protein Khd1 interacts with the ASH1 mRNA localization element E1 and with the C-terminal domain of eIF4G1 to regulate the ..
  2. Irie K, Tadauchi T, Takizawa P, Vale R, Matsumoto K, Herskowitz I. The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast. EMBO J. 2002;21:1158-67 pubmed
    ..We show here that a novel RNA-binding protein, Khd1 (KH-domain protein 1), is required for efficient localization of ASH1 mRNA to the distal tip of the daughter cell...
  3. Tadauchi T, Inada T, Matsumoto K, Irie K. Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Mol Cell Biol. 2004;24:3670-81 pubmed
    ..These observations suggest that a complex of Mkt1 and Pbp1 regulates the translation of HO mRNA. ..
  4. Denisenko O, Bomsztyk K. Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast. J Mol Biol. 2008;375:1178-87 pubmed
    ..To explore the mechanisms of Hek2p action in gene silencing, we examined its relationship with Sir proteins...
  5. Mauchi N, Ohtake Y, Irie K. Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast. Cell Struct Funct. 2010;35:95-105 pubmed
    ..that partial deletion of the coding sequences of MTL1 mRNA restored the decreased MTL1 mRNA and protein levels in khd1? mutants...
  6. Ito W, Li X, Irie K, Mizuno T, Irie K. RNA-binding protein Khd1 and Ccr4 deadenylase play overlapping roles in the cell wall integrity pathway in Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:1340-7 pubmed publisher
    The Saccharomyces cerevisiae RNA-binding protein Khd1/Hek2 associates with hundreds of potential mRNA targets preferentially, including the mRNAs encoding proteins localized to the cell wall and plasma membrane...
  7. McDonald R, Guilinger J, Mukherji S, Curtis E, Lee W, Liu D. Electrophilic activity-based RNA probes reveal a self-alkylating RNA for RNA labeling. Nat Chem Biol. 2014;10:1049-54 pubmed publisher
    ..This strategy enabled up to 500-fold enrichment of target RNA from total mammalian RNA or from cell lysate. We demonstrated the utility of this approach by selectively capturing proteins in yeast cell lysate that bind the ASH1 mRNA. ..
  8. Li X, Ohmori T, Irie K, Kimura Y, Suda Y, Mizuno T, et al. Different Regulations of ROM2 and LRG1 Expression by Ccr4, Pop2, and Dhh1 in the Saccharomyces cerevisiae Cell Wall Integrity Pathway. mSphere. 2016;1: pubmed
    ..Previously, we showed that Ccr4 functions in the CWI pathway together with Khd1 RNA binding protein...
  9. Denisenko O, Bomsztyk K. Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length. Mol Cell Biol. 2002;22:286-97 pubmed
    ..b>Hek2p was detected in a complex with chromosome regions proximal to the affected telomere, suggesting a direct ..

More Information


  1. Wolf J, Dowell R, Mahony S, Rabani M, Gifford D, Fink G. Feed-forward regulation of a cell fate determinant by an RNA-binding protein generates asymmetry in yeast. Genetics. 2010;185:513-22 pubmed publisher
    ..We show that the RNA-binding protein Khd1 regulates asymmetric expression of FLO11 to determine daughter cell fate during filamentous growth...
  2. Tsvetanova N, Klass D, Salzman J, Brown P. Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae. PLoS ONE. 2010;5: pubmed publisher
    ..Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation. ..
  3. Kimura Y, Irie K, Irie K. Pbp1 is involved in Ccr4- and Khd1-mediated regulation of cell growth through association with ribosomal proteins Rpl12a and Rpl12b. Eukaryot Cell. 2013;12:864-74 pubmed publisher
    ..In this study, we showed that PBP1 genetically interacts with CCR4 and KHD1, which encode a cytoplasmic deadenylase and an RNA-binding protein, respectively...
  4. Hirschmann W, Westendorf H, Mayer A, Cannarozzi G, Cramer P, Jansen R. Scp160p is required for translational efficiency of codon-optimized mRNAs in yeast. Nucleic Acids Res. 2014;42:4043-55 pubmed publisher
    ..Our data indicate that Scp160p might increase the efficiency of tRNA recharge, or prevent diffusion of discharged tRNAs, both of which were also proposed to be the likely basis for the translational fitness effect of tRNA pairing...