GTR1

Summary

Gene Symbol: GTR1
Description: Rag GTPase GTR1
Alias: Rag GTPase GTR1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lagerstedt J, Reeve I, Voss J, Persson B. Structure and function of the GTP binding protein Gtr1 and its role in phosphate transport in Saccharomyces cerevisiae. Biochemistry. 2005;44:511-7 pubmed
    ..The soluble G protein, Gtr1, has previously been suggested to be involved in the derepressible Pho84 phosphate uptake function...
  2. Sekiguchi T, Hayashi N, Wang Y, Kobayashi H. Genetic evidence that Ras-like GTPases, Gtr1p, and Gtr2p, are involved in epigenetic control of gene expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2008;368:748-54 pubmed publisher
    ..We propose that Gtr1p and Gtr2p are involved in epigenetic control of gene expression in the TOR signaling pathway. ..
  3. Bun Ya M, Harashima S, Oshima Y. Putative GTP-binding protein, Gtr1, associated with the function of the Pho84 inorganic phosphate transporter in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:2958-66 pubmed
    ..In Saccharomyces cerevisiae, this gene is distal to the TUB3 locus on the left arm of chromosome XIII and is named GTR1. GTR1 encodes a protein consisting of 310 amino acid residues containing, in its N-terminal region, the ..
  4. Binda M, Péli Gulli M, Bonfils G, Panchaud N, Urban J, Sturgill T, et al. The Vam6 GEF controls TORC1 by activating the EGO complex. Mol Cell. 2009;35:563-73 pubmed publisher
    ..g., amino acids) and is deregulated in various cancers. Here, we report that the yeast Rag GTPase homolog Gtr1, a component of the vacuolar-membrane-associated EGO complex (EGOC), interacts with and activates TORC1 in an amino-..
  5. Gao X, Wang J, Keppler Ross S, Dean N. ERS1 encodes a functional homologue of the human lysosomal cystine transporter. FEBS J. 2005;272:2497-511 pubmed
    ..This genetic system has also led us to identify Gtr1 as an Meh1 interacting protein...
  6. Gong R, Li L, Liu Y, Wang P, Yang H, Wang L, et al. Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation. Genes Dev. 2011;25:1668-73 pubmed publisher
    ..Our results reveal a structural model for the mechanism of the Rag GTPases in TORC1 activation and amino acid signaling. ..
  7. Todaka Y, Wang Y, Tashiro K, Nakashima N, Nishimoto T, Sekiguchi T. Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae. Genetics. 2005;170:1515-24 pubmed
    ..Here, we propose that Gtr1p is involved in RNA polymerase I and III assembly by its association with Rpc19p and could be a mediator that links growth regulatory signals with ribosome biogenesis. ..
  8. Wang Y, Nakashima N, Sekiguchi T, Nishimoto T. Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p. Biochem Biophys Res Commun. 2005;336:639-45 pubmed
    ..The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p. ..
  9. Gao M, Kaiser C. A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol. 2006;8:657-67 pubmed
    ..Together, these studies provide evidence that the GSE complex has a key role in trafficking Gap1p out of the endosome and may serve as coat proteins in this process. ..

More Information

Publications34

  1. Nakashima N, Hayashi N, Noguchi E, Nishimoto T. Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae. J Cell Sci. 1996;109 ( Pt 9):2311-8 pubmed
    ..One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S...
  2. Nakashima N, Noguchi E, Nishimoto T. Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p. Genetics. 1999;152:853-67 pubmed
    ..of Gsp1p, respectively, and their mutations, prp20-1 and rna1-1, can both be suppressed by Saccharomyces cerevisiae gtr1-11...
  3. Zurita Martinez S, Puria R, Pan X, Boeke J, Cardenas M. Efficient Tor signaling requires a functional class C Vps protein complex in Saccharomyces cerevisiae. Genetics. 2007;176:2139-50 pubmed
  4. Kingsbury J, Sen N, Cardenas M. Branched-Chain Aminotransferases Control TORC1 Signaling in Saccharomyces cerevisiae. PLoS Genet. 2015;11:e1005714 pubmed publisher
  5. Kim A, Cunningham K. A LAPF/phafin1-like protein regulates TORC1 and lysosomal membrane permeabilization in response to endoplasmic reticulum membrane stress. Mol Biol Cell. 2015;26:4631-45 pubmed publisher
    ..kinase that is sensitive to rapamycin, in a pathway parallel to the Ragulator/EGO complex containing the GTPases Gtr1 and Gtr2...
  6. Hurto R, Tong A, Boone C, Hopper A. Inorganic phosphate deprivation causes tRNA nuclear accumulation via retrograde transport in Saccharomyces cerevisiae. Genetics. 2007;176:841-52 pubmed
  7. Stracka D, Jozefczuk S, Rudroff F, Sauer U, Hall M. Nitrogen source activates TOR (target of rapamycin) complex 1 via glutamine and independently of Gtr/Rag proteins. J Biol Chem. 2014;289:25010-20 pubmed publisher
    ..Leucine, and perhaps other amino acids, activates TORC1 via the small GTPases Gtr1 and Gtr2, orthologs of the mammalian Rag GTPases...
  8. Takahara T, Maeda T. Transient sequestration of TORC1 into stress granules during heat stress. Mol Cell. 2012;47:242-52 pubmed publisher
    ..Furthermore, this mechanism contributes to reduction of heat-induced mutations. Thus, TORC1 signaling is coupled to heat-induced SGs to protect cells from DNA damage...
  9. Kira S, Kumano Y, Ukai H, Takeda E, Matsuura A, Noda T. Dynamic relocation of the TORC1-Gtr1/2-Ego1/2/3 complex is regulated by Gtr1 and Gtr2. Mol Biol Cell. 2016;27:382-96 pubmed publisher
    ..integrating various signals, including nutrient availability, through the small GTPases RagA/B/C/D in mammals and Gtr1/2 in budding yeast...
  10. Numrich J, Péli Gulli M, Arlt H, Sardu A, Griffith J, Levine T, et al. The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis. J Cell Sci. 2015;128:2278-92 pubmed publisher
    ..Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling. ..
  11. Zhang T, Péli Gulli M, Yang H, De Virgilio C, Ding J. Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the ego complex to activate TORC1. Structure. 2012;20:2151-60 pubmed publisher
    The yeast EGO complex, consisting of Gtr1, Gtr2, Ego1, and Ego3, localizes to the endosomal and vacuolar membranes and plays a pivotal role in cell growth and autophagy regulation through relaying amino acid signals to activate TORC1...
  12. MacDonald C, Piper R. Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases. J Cell Biol. 2017;216:3275-3290 pubmed publisher
    ..exclusively follows this pathway revealed that recycling was subject to metabolic control through the Rag GTPases Gtr1 and Gtr2, which work downstream of the exchange factor Vam6...
  13. Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C, Ding J. Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling. Cell Res. 2015;25:1043-59 pubmed publisher
    ..Amino acid-dependent activation of TORC1 is mediated via the yeast EGO complex (EGOC) consisting of Gtr1, Gtr2, Ego1, and Ego3...
  14. Kira S, Tabata K, Shirahama Noda K, Nozoe A, Yoshimori T, Noda T. Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy. Autophagy. 2014;10:1565-78 pubmed publisher
    ..Npr2-Npr3 function upstream of Gtr1-Gtr2, homologs of the mammalian RRAG GTPase complex, which is crucial for TORC1 regulation...
  15. Sengottaiyan P, Spetea C, Lagerstedt J, Samyn D, Andersson M, Ruiz Pavón L, et al. The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae. BMC Biochem. 2012;13:11 pubmed publisher
    The Gtr1 protein of Saccharomyces cerevisiae is a member of the RagA subfamily of the Ras-like small GTPase superfamily. Gtr1 has been implicated in various cellular processes...
  16. Sekiguchi T, Kamada Y, Furuno N, Funakoshi M, Kobayashi H. Amino acid residues required for Gtr1p-Gtr2p complex formation and its interactions with the Ego1p-Ego3p complex and TORC1 components in yeast. Genes Cells. 2014;19:449-63 pubmed publisher
    ..The GTP-bound Gtr2p suppressed a Kog1p mutation. Our findings indicate that the interactions of the Gtr1p-Gtr2p complex with the Ego1p-Ego3p complex and TORC1 components Kog1p and Tco89p play a role in TORC1 function. ..
  17. Dechant R, Saad S, Ibáñez A, Peter M. Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Mol Cell. 2014;55:409-21 pubmed publisher
    ..V-ATPase interacts with two distinct GTPases, Arf1 and Gtr1, which are required for Ras and TORC1 activation, respectively...
  18. Jeong J, Lee K, Kim Y, Kim D, Oh B, Kim Y. Crystal structure of the Gtr1p(GTP)-Gtr2p(GDP) protein complex reveals large structural rearrangements triggered by GTP-to-GDP conversion. J Biol Chem. 2012;287:29648-53 pubmed publisher
    ..These features explain how the nucleotide-bound statuses of the two GTPases subunits switch the Raptor binding affinity on and off. ..
  19. Hess D, Winston F. Evidence that Spt10 and Spt21 of Saccharomyces cerevisiae play distinct roles in vivo and functionally interact with MCB-binding factor, SCB-binding factor and Snf1. Genetics. 2005;170:87-94 pubmed
    ..Taken together, these genetic interactions suggest distinct roles for Spt21 and Spt10 in vivo that are sensitive to multiple perturbations in transcription networks. ..
  20. Kingsbury J, Sen N, Maeda T, Heitman J, Cardenas M. Endolysosomal membrane trafficking complexes drive nutrient-dependent TORC1 signaling to control cell growth in Saccharomyces cerevisiae. Genetics. 2014;196:1077-89 pubmed publisher
    ..Expression of the activated EGOC GTPase subunits Gtr1(GTP) and Gtr2(GDP) partially suppressed vps-c mutant rapamycin recovery defects, and this suppression was enhanced ..
  21. Panchaud N, Péli Gulli M, De Virgilio C. Amino acid deprivation inhibits TORC1 through a GTPase-activating protein complex for the Rag family GTPase Gtr1. Sci Signal. 2013;6:ra42 pubmed publisher
    ..In yeast, amino acids promote binding of GTP (guanosine 5'-triphosphate) to the Rag family GTPase Gtr1, which, in combination with a GDP (guanosine diphosphate)-bound Gtr2, forms the active, TORC1-stimulating GTPase ..
  22. Bonfils G, Jaquenoud M, Bontron S, Ostrowicz C, Ungermann C, De Virgilio C. Leucyl-tRNA synthetase controls TORC1 via the EGO complex. Mol Cell. 2012;46:105-10 pubmed publisher
    ..We find that the leucyl-tRNA synthetase (LeuRS) Cdc60 interacts with the Rag GTPase Gtr1 of the EGOC in a leucine-dependent manner...
  23. Copic A, Latham C, Horlbeck M, D Arcangelo J, Miller E. ER cargo properties specify a requirement for COPII coat rigidity mediated by Sec13p. Science. 2012;335:1359-62 pubmed publisher
    ..Thus, Sec13p may rigidify the COPII cage and increase its membrane-bending capacity; this function could be bypassed when a bst mutation renders the membrane more deformable. ..
  24. Péli Gulli M, Sardu A, Panchaud N, Raucci S, De Virgilio C. Amino Acids Stimulate TORC1 through Lst4-Lst7, a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr2. Cell Rep. 2015;13:1-7 pubmed publisher
    ..GTPases assemble into heterodimeric complexes consisting of RagA or RagB and RagC or RagD in higher eukaryotes, or Gtr1 and Gtr2 in yeast, to relay amino acid signals toward the growth-regulating target of rapamycin complex 1 (TORC1)...
  25. Wang Y, Kurihara Y, Sato T, Toh H, Kobayashi H, Sekiguchi T. Gtr1p differentially associates with Gtr2p and Ego1p. Gene. 2009;437:32-8 pubmed
    ..Gtr2p mutants S23N and T44N, but not Q66L, rescued the gtr2 disruptant. Our findings indicate that the formation of heterodimers by Gtr1p differs between Gtr2p and Ego1p. ..