Gene Symbol: GPB2
Alias: KRH1, Gpb2p
Species: Saccharomyces cerevisiae S288c
Harashima T, Heitman J. The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell. 2002;10:163-73 pubmed
..Gpb1 and Gpb2 lack the seven WD-40 repeats found in Gbeta subunits and instead contain seven kelch repeats implicated in protein-..
Batlle M, Lu A, Green D, Xue Y, Hirsch J. Krh1p and Krh2p act downstream of the Gpa2p G(alpha) subunit to negatively regulate haploid invasive growth. J Cell Sci. 2003;116:701-10 pubmed
..A two-hybrid screen using a constitutively active allele of GPA2 identified the KRH1 gene as encoding a potential binding partner of Gpa2p...
Lu A, Hirsch J. Cyclic AMP-independent regulation of protein kinase A substrate phosphorylation by Kelch repeat proteins. Eukaryot Cell. 2005;4:1794-800 pubmed
..growth in the yeast Saccharomyces cerevisiae is regulated by the kelch repeat-containing proteins Gpb1p and Gpb2p, which act downstream of the G protein alpha-subunit Gpa2p...
Harashima T, Heitman J. Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:4557-71 pubmed
..Galpha subunits, Gpa2 forms an atypical G protein complex with the kelch repeat Gbeta mimic proteins Gpb1 and Gpb2. Gpb1/2 negatively regulate cAMP signaling by inhibiting Gpa2 and an as yet unidentified target...
Harashima T, Anderson S, Yates J, Heitman J. The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2. Mol Cell. 2006;22:819-30 pubmed
..Because the Gpb1/2 binding domain on Ira1/2 is conserved in the human neurofibromin protein, an analogous signaling network may contribute to the neoplastic development of neurofibromatosis type 1. ..
Niranjan T, Guo X, Victor J, Lu A, Hirsch J. Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange. J Biol Chem. 2007;282:24231-8 pubmed
..The results suggest that binding of Gpa2p to Krh1p does not resemble the binding of Galpha subunits to either Gbeta subunits or effectors, but it instead represents a novel type of functional interaction. ..
Peeters T, Louwet W, Geladé R, Nauwelaers D, Thevelein J, Versele M. Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast. Proc Natl Acad Sci U S A. 2006;103:13034-9 pubmed
..Two related kelch-repeat proteins, Krh1/Gpb2 and Krh2/Gpb1, are associated with Gpa2 and were suggested to act as G(beta) mimics for Gpa2, based on their ..
Enyenihi A, Saunders W. Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae. Genetics. 2003;163:47-54 pubmed
..The set of genes identified in this phenotypic screen shows only limited overlap with those identified by expression-based studies. ..
Budhwar R, Lu A, Hirsch J. Nutrient control of yeast PKA activity involves opposing effects on phosphorylation of the Bcy1 regulatory subunit. Mol Biol Cell. 2010;21:3749-58 pubmed publisher
GPB1 and GPB2 encode kelch repeat-containing proteins that regulate protein kinase A (PKA) in yeast by a cAMP-independent process...