Gene Symbol: GLE2
Description: RNA export factor GLE2
Alias: RAE1, RNA export factor GLE2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bailer S, Siniossoglou S, Podtelejnikov A, Hellwig A, Mann M, Hurt E. Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p. EMBO J. 1998;17:1107-19 pubmed
    ..Thus, the in vivo function of Gle2p is strictly coupled to the short GLEBS within Nup116p which links this putative mRNA transport factor to the nuclear pores. ..
  2. Miao M, Ryan K, Wente S. The integral membrane protein Pom34p functionally links nucleoporin subcomplexes. Genetics. 2006;172:1441-57 pubmed
    ..We speculate that multiple integral membrane proteins, either within the nuclear pore domain or in the nuclear envelope, execute coordinated roles in NPC structure and function. ..
  3. Murphy R, Watkins J, Wente S. GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell. 1996;7:1921-37 pubmed
    ..In this report, the characterization of GLE2 is detailed. GLE2 encodes a 40.5-kDa polypeptide with striking similarity to that of Schizosaccharomyces pombe RAE1...
  4. Hung N, Lo K, Patel S, Helmke K, Johnson A. Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast. Mol Biol Cell. 2008;19:735-44 pubmed
    ..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast. ..
  5. Ho A, Raczniak G, Ives E, Wente S. The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function. Mol Biol Cell. 1998;9:355-73 pubmed
    ..With regard to genetic specificity, the nup116-C lethality was also suppressed by high-copy GLE2 and NIC96. Moreover, high-copy SNL1 suppressed the temperature sensitivity of gle2-1 and nic96-G3 mutant cells...
  6. Bucci M, Wente S. A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Mol Biol Cell. 1998;9:2439-61 pubmed
    ..cells expressing GFP-Nup49p, three complementation groups were identified: two correspond to mutant nup120 and gle2 alleles that result in clusters of NPCs...
  7. Izawa S, Takemura R, Inoue Y. Gle2p is essential to induce adaptation of the export of bulk poly(A)+ mRNA to heat shock in Saccharomyces cerevisiae. J Biol Chem. 2004;279:35469-78 pubmed
  8. Bailer S, Balduf C, Katahira J, Podtelejnikov A, Rollenhagen C, Mann M, et al. Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J Biol Chem. 2000;275:23540-8 pubmed
  9. Fischer T, Strasser K, Rácz A, Rodriguez Navarro S, Oppizzi M, Ihrig P, et al. The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. EMBO J. 2002;21:5843-52 pubmed
    ..Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC. ..

More Information


  1. Zander G, Kramer W, Seel A, Krebber H. Saccharomyces cerevisiae Gle2/Rae1 is involved in septin organization, essential for cell cycle progression. Yeast. 2017;34:459-470 pubmed publisher
    b>Gle2/Rae1 is highly conserved from yeast to humans and has been described as an mRNA export factor. Additionally, it is implicated in the anaphase-promoting complex-mediated cell cycle regulation in higher eukaryotes...
  2. Dawson T, Lazarus M, Hetzer M, Wente S. ER membrane-bending proteins are necessary for de novo nuclear pore formation. J Cell Biol. 2009;184:659-75 pubmed publisher
    ..We hypothesize that these ER membrane-bending proteins mediate early NPC assembly steps...
  3. Ahmed S, Brickner D, Light W, Cajigas I, McDonough M, Froyshteter A, et al. DNA zip codes control an ancient mechanism for gene targeting to the nuclear periphery. Nat Cell Biol. 2010;12:111-8 pubmed publisher
    ..Finally, GRS I also functions as a DNA zip code in Schizosaccharomyces pombe, suggesting that this mechanism of targeting to the nuclear periphery has been conserved over approximately one billion years of evolution. ..
  4. Fernandez Martinez J, Kim S, Shi Y, Upla P, Pellarin R, Gagnon M, et al. Structure and Function of the Nuclear Pore Complex Cytoplasmic mRNA Export Platform. Cell. 2016;167:1215-1228.e25 pubmed publisher
    ..We suggest that this configuration efficiently captures and remodels exporting mRNP particles immediately upon reaching the cytoplasmic side of the NPC. ..
  5. Fabre E, Hurt E. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet. 1997;31:277-313 pubmed
    ..We review here our current knowledge on the role of nucleoporins, and on the mechanism of nucleocytoplasmic transport, with emphasis on the yeast Saccharomyces cerevisiae. ..
  6. Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher
    ..Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S)...
  7. Miller A, Suntharalingam M, Johnson S, Audhya A, Emr S, Wente S. Cytoplasmic inositol hexakisphosphate production is sufficient for mediating the Gle1-mRNA export pathway. J Biol Chem. 2004;279:51022-32 pubmed
    ..Enhanced lethality was observed with a specific subset of mutants, including nup42, nup116, nup159, dbp5, and gle2, all of which had been previously connected to Gle1 function...
  8. Senissar M, Le Saux A, Belgareh Touzé N, Adam C, Banroques J, Tanner N. The DEAD-box helicase Ded1 from yeast is an mRNP cap-associated protein that shuttles between the cytoplasm and nucleus. Nucleic Acids Res. 2014;42:10005-22 pubmed publisher
    ..We conclude that Ded1 is an mRNP cofactor of the cap complex that may function to remodel the different mRNPs and thereby regulate the expression of the mRNAs. ..
  9. Light W, Brickner D, Brand V, Brickner J. Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell. 2010;40:112-25 pubmed publisher
    ..Thus, interaction of recently repressed INO1 with the NPC alters its chromatin structure and rate of reactivation...
  10. Iovine M, Wente S. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol. 1997;137:797-811 pubmed
    ..This finding highlights an important role for a subfamily of GLFG nucleoporins in nuclear export processes. ..