GET2

Summary

Gene Symbol: GET2
Description: GET complex subunit GET2
Alias: HUR2, RMD7, GET complex subunit GET2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Auld K, Hitchcock A, Doherty H, Frietze S, Huang L, Silver P. The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae. Genetics. 2006;174:215-27 pubmed
    ..We have further found that Get3 biochemically interacts with the trans-membrane domain proteins Get1/Mdm39 and Get2/Rmd7 and that Deltaget3 is able to suppress phenotypes of get1 and get2 mutants, including sporulation defects...
  2. Mariappan M, Mateja A, Dobosz M, Bove E, Hegde R, Keenan R. The mechanism of membrane-associated steps in tail-anchored protein insertion. Nature. 2011;477:61-6 pubmed publisher
    ..a TA protein bound to the chaperone ATPase Get3, is initially recruited to the membrane through an interaction with Get2. Once the targeting complex has been recruited, Get1 interacts with Get3 to drive TA protein release in an ATPase-..
  3. Kiktev D, Patterson J, Muller S, Bariar B, Pan T, Chernoff Y. Regulation of chaperone effects on a yeast prion by cochaperone Sgt2. Mol Cell Biol. 2012;32:4960-70 pubmed publisher
    ..Our data implicate Sgt2 as an amyloid "sensor" and a regulator of chaperone targeting to different types of aggregation-prone proteins. ..
  4. Wang F, Chan C, Weir N, Denic V. The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase. Nature. 2014;512:441-4 pubmed publisher
  5. Holbein S, Wengi A, Decourty L, Freimoser F, Jacquier A, Dichtl B. Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation. RNA. 2009;15:837-49 pubmed publisher
    ..Finally, chemical-genetic profiling revealed genome-wide pathways linked to cordycepin activity and identified novel genes involved in poly(A) homeostasis. ..
  6. Stefer S, Reitz S, Wang F, Wild K, Pang Y, Schwarz D, et al. Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science. 2011;333:758-62 pubmed publisher
    ..We have determined crystal structures of Get3 in complex with the cytosolic domains of Get1 and Get2 in different functional states at 3.0, 3.2, and 4.6 angstrom resolution...
  7. Schuldiner M, Metz J, Schmid V, Denic V, Rakwalska M, Schmitt H, et al. The GET complex mediates insertion of tail-anchored proteins into the ER membrane. Cell. 2008;134:634-45 pubmed publisher
    ..Get3-TA protein complexes are recruited for endoplasmic reticulum (ER) membrane insertion by the Get1/Get2 receptor...
  8. Garbarino J, Padamsee M, Wilcox L, Oelkers P, D Ambrosio D, Ruggles K, et al. Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death. J Biol Chem. 2009;284:30994-1005 pubmed publisher
    ..In metazoans, many of these processes are implicated in insulin secretion thus linking lipotoxicity with early aspects of pancreatic beta-cell dysfunction, diabetes, and the metabolic syndrome. ..
  9. Wang F, Whynot A, Tung M, Denic V. The mechanism of tail-anchored protein insertion into the ER membrane. Mol Cell. 2011;43:738-50 pubmed publisher
    ..Get3 is an ATPase that delivers TA proteins to the ER by interacting with the Get1-Get2 transmembrane complex, but how Get3's nucleotide cycle drives TA protein insertion remains unclear...

More Information

Publications15

  1. Takahashi H, McCaffery J, Irizarry R, Boeke J. Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription. Mol Cell. 2006;23:207-17 pubmed
    ..Thus, acetyl-CoA metabolism is directly linked to chromatin regulation and may affect diverse cellular processes in which acetylation and metabolism intersect, such as disease states and aging. ..
  2. Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
    ..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
  3. Okreglak V, Walter P. The conserved AAA-ATPase Msp1 confers organelle specificity to tail-anchored proteins. Proc Natl Acad Sci U S A. 2014;111:8019-24 pubmed publisher
    ..Our data suggest that Msp1 functions in local organelle surveillance by extracting mistargeted proteins, ensuring the fidelity of organelle specific-localization of TA proteins. ..
  4. Lafourcade C, Galan J, Gloor Y, Haguenauer Tsapis R, Peter M. The GTPase-activating enzyme Gyp1p is required for recycling of internalized membrane material by inactivation of the Rab/Ypt GTPase Ypt1p. Mol Cell Biol. 2004;24:3815-26 pubmed
    ..Interestingly, the deletions of GYP1 and YPT6 were synthetic lethal, raising the possibility that at least two distinct pathways are involved in recycling of membrane material. ..
  5. Chen Y, Umanah G, Dephoure N, Andrabi S, Gygi S, Dawson T, et al. Msp1/ATAD1 maintains mitochondrial function by facilitating the degradation of mislocalized tail-anchored proteins. EMBO J. 2014;33:1548-64 pubmed publisher
  6. Joshi A, Fei N, Greenberg M. Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels. FEMS Yeast Res. 2016;16: pubmed publisher
    ..The genetic interactions observed in this study identify novel physiological modifiers that are required for maintenance of CL levels and mitochondrial morphology. ..