Gene Symbol: GEA2
Description: Arf family guanine nucleotide exchange factor GEA2
Alias: Arf family guanine nucleotide exchange factor GEA2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Deng Y, Golinelli Cohen M, Smirnova E, Jackson C. A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factor. EMBO Rep. 2009;10:58-64 pubmed publisher
    ..In this way, a specific output on Arf1 activation can be programmed before the exchange reaction by the GEF itself. ..
  2. Natarajan P, Liu K, Patil D, Sciorra V, Jackson C, Graham T. Regulation of a Golgi flippase by phosphoinositides and an ArfGEF. Nat Cell Biol. 2009;11:1421-6 pubmed publisher
    ..These interactions suggest the presence of a coincidence detection system used to activate phospholipid translocation at sites of vesicle formation. ..
  3. Peyroche A, Courbeyrette R, Rambourg A, Jackson C. The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast. J Cell Sci. 2001;114:2241-53 pubmed
    ..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast. ..
  4. Chantalat S, Courbeyrette R, Senic Matuglia F, Jackson C, Goud B, Peyroche A. A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p. Mol Biol Cell. 2003;14:2357-71 pubmed
    ..Hence, we propose that Gmh1p acts as a positive Golgi-membrane partner for Gea function. These results are of general interest given the evolutionary conservation of both ARF-GEFs and the Gmh proteins. ..
  5. Chantalat S, Park S, Hua Z, Liu K, Gobin R, Peyroche A, et al. The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae. J Cell Sci. 2004;117:711-22 pubmed
    ..We characterize one such mutant that has a thermosensitive phenotype, and show that it has morphological defects along the secretory pathway in the formation of secretory granules/vesicles. ..
  6. Jones S, Jedd G, Kahn R, Franzusoff A, Bartolini F, Segev N. Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics. 1999;152:1543-56 pubmed
    ..The genetic interactions observed between Arf GEFs and Ypt GTPases suggest the existence of a Ypt-Arf GTPase cascade in the secretory pathway. ..
  7. Chen K, Tsai P, Hsu J, Hsu H, Fang C, Chang L, et al. Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p. J Cell Sci. 2010;123:3478-89 pubmed publisher
    ..These findings reveal that activation of Arl1p is regulated in part by Syt1p, and imply that Arl1p activation, by using more than one GEF, exerts distinct biological activities at the Golgi compartment. ..
  8. Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5021-37 pubmed
    ..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER. ..
  9. Trautwein M, Schindler C, Gauss R, Dengjel J, Hartmann E, Spang A. Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi. EMBO J. 2006;25:943-54 pubmed
    ..In addition, they are all at least partially localized to the TGN in a Chs5p-dependent manner. Most importantly, several ChAPs can interact physically with Chs3p. We propose that the ChAPs facilitate export of cargo out of the Golgi...

More Information


  1. Brett C, Plemel R, Lobingier B, Lobinger B, Vignali M, Fields S, et al. Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase. J Cell Biol. 2008;182:1141-51 pubmed publisher
    ..We propose that signal propagation through the Ypt7 pathway is controlled by integrated feedback and feed-forward loops. In this model, Yck3 enforces a requirement for the activated Rab in docking and fusion. ..
  2. Tsai P, Hsu J, Liu Y, Chen K, Lee F. Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p. Proc Natl Acad Sci U S A. 2013;110:E668-77 pubmed publisher
    ..Our results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p. ..
  3. Anders N, Nielsen M, Keicher J, Stierhof Y, Furutani M, Tasaka M, et al. Membrane association of the Arabidopsis ARF exchange factor GNOM involves interaction of conserved domains. Plant Cell. 2008;20:142-51 pubmed publisher
    ..Our results suggest a general model of large ARF-GEF function in which regulated changes in protein conformation control membrane association of the exchange factor and, thus, activation of ARFs. ..
  4. Gloor Y, Schöne M, Habermann B, Ercan E, Beck M, Weselek G, et al. Interaction between Sec7p and Pik1p: the first clue for the regulation of a coincidence detection signal. Eur J Cell Biol. 2010;89:575-83 pubmed publisher
  5. Lambert A, Perron M, Lavoie E, Pallotta D. The Saccharomyces cerevisiae Arf3 protein is involved in actin cable and cortical patch formation. FEMS Yeast Res. 2007;7:782-95 pubmed
    ..arf3Delta cells show a random budding pattern. Overexpression of BNI1, GEA2 or SYP1, three genes involved in actin cytoskeleton formation, restores the normal axial budding pattern of ..
  6. Chen S, Cai H, Park S, Menon S, Jackson C, Ferro Novick S. Trs65p, a subunit of the Ypt1p GEF TRAPPII, interacts with the Arf1p exchange factor Gea2p to facilitate COPI-mediated vesicle traffic. Mol Biol Cell. 2011;22:3634-44 pubmed publisher
    ..In support of this proposal, we show that TRAPPII is more soluble in an arf1? mutant. ..
  7. van der Vaart A, Griffith J, Reggiori F. Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2270-84 pubmed publisher
    ..and have determined that in yeast, activation of ADP-ribosylation factor (Arf)1 and Arf2 GTPases by Sec7, Gea1, and Gea2 is essential for this catabolic process...
  8. Park S, Hartnell L, Jackson C. Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes. Mol Biol Cell. 2005;16:3786-99 pubmed
    ..These gea2-ts mutants block or slow transport of proteins traversing the secretory pathway at exit from the endoplasmic ..
  9. Zakrzewska E, Perron M, Laroche A, Pallotta D. A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae. Genetics. 2003;165:985-95 pubmed
    ..The GEA1 and GEA2 genes, which code for paralog guanosine exchange factors for Arf proteins, were identified as multicopy suppressors ..
  10. Spang A, Herrmann J, Hamamoto S, Schekman R. The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum. Mol Biol Cell. 2001;12:1035-45 pubmed
    ..We identified GEA2 as a multicopy suppressor of a sec21-3 temperature-sensitive mutant...