GEA1

Summary

Gene Symbol: GEA1
Description: Arf family guanine nucleotide exchange factor GEA1
Alias: Arf family guanine nucleotide exchange factor GEA1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Peyroche A, Paris S, Jackson C. Nucleotide exchange on ARF mediated by yeast Gea1 protein. Nature. 1996;384:479-81 pubmed
    ..This nucleotide exchange is inhibited by the fungal metabolite brefeldin A (BFA). Here we identify a protein (Gea1) from Saccharomyces cerevisiae that is a component of a complex possessing guanine-nucleotide-exchange activity for ..
  2. Peyroche A, Courbeyrette R, Rambourg A, Jackson C. The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast. J Cell Sci. 2001;114:2241-53 pubmed
    ..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast. ..
  3. Jones S, Jedd G, Kahn R, Franzusoff A, Bartolini F, Segev N. Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics. 1999;152:1543-56 pubmed
    ..The genetic interactions observed between Arf GEFs and Ypt GTPases suggest the existence of a Ypt-Arf GTPase cascade in the secretory pathway. ..
  4. Chantalat S, Courbeyrette R, Senic Matuglia F, Jackson C, Goud B, Peyroche A. A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p. Mol Biol Cell. 2003;14:2357-71 pubmed
    ..Hence, we propose that Gmh1p acts as a positive Golgi-membrane partner for Gea function. These results are of general interest given the evolutionary conservation of both ARF-GEFs and the Gmh proteins. ..
  5. Deng Y, Golinelli Cohen M, Smirnova E, Jackson C. A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factor. EMBO Rep. 2009;10:58-64 pubmed publisher
    ..In this way, a specific output on Arf1 activation can be programmed before the exchange reaction by the GEF itself. ..
  6. van der Vaart A, Griffith J, Reggiori F. Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2270-84 pubmed publisher
    ..autophagy and have determined that in yeast, activation of ADP-ribosylation factor (Arf)1 and Arf2 GTPases by Sec7, Gea1, and Gea2 is essential for this catabolic process...
  7. Chen S, Cai H, Park S, Menon S, Jackson C, Ferro Novick S. Trs65p, a subunit of the Ypt1p GEF TRAPPII, interacts with the Arf1p exchange factor Gea2p to facilitate COPI-mediated vesicle traffic. Mol Biol Cell. 2011;22:3634-44 pubmed publisher
    ..In support of this proposal, we show that TRAPPII is more soluble in an arf1? mutant. ..
  8. Spang A, Herrmann J, Hamamoto S, Schekman R. The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum. Mol Biol Cell. 2001;12:1035-45 pubmed
    ..b>GEA1 and GEA2 have at least partially overlapping functions, because deletion of either gene results in no obvious ..
  9. Zakrzewska E, Perron M, Laroche A, Pallotta D. A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae. Genetics. 2003;165:985-95 pubmed
    ..The GEA1 and GEA2 genes, which code for paralog guanosine exchange factors for Arf proteins, were identified as multicopy ..

More Information

Publications15

  1. Tsai P, Hsu J, Liu Y, Chen K, Lee F. Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p. Proc Natl Acad Sci U S A. 2013;110:E668-77 pubmed publisher
    ..Our results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p. ..
  2. Chapa y Lazo B, Ayscough K. Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites. Commun Integr Biol. 2014;7:e28522 pubmed publisher
  3. Mossessova E, Corpina R, Goldberg J. Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism. Mol Cell. 2003;12:1403-11 pubmed
    ..Thus, the Sec7 domain may act as a dual catalyst, facilitating both nucleotide release and conformational switching on ARF proteins. ..
  4. Park S, Hartnell L, Jackson C. Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes. Mol Biol Cell. 2005;16:3786-99 pubmed
    ..Hence this region of Gea2p upstream of the Sec7 domain plays a role in anterograde transport that is independent of its role in recruiting COPI for retrograde transport, at least of a subset of Golgi-ER cargo. ..
  5. Anders N, Nielsen M, Keicher J, Stierhof Y, Furutani M, Tasaka M, et al. Membrane association of the Arabidopsis ARF exchange factor GNOM involves interaction of conserved domains. Plant Cell. 2008;20:142-51 pubmed publisher
    ..Our results suggest a general model of large ARF-GEF function in which regulated changes in protein conformation control membrane association of the exchange factor and, thus, activation of ARFs. ..
  6. Aucher W, Becker E, Ma E, Miron S, Martel A, Ochsenbein F, et al. A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data. Mol Cell Proteomics. 2010;9:2745-59 pubmed publisher
    ..The STRIP method relies on a combination of conservation, phosphorylation likelihood, and binding specificity criteria and can be accessed via a web interface at http://biodev.extra.cea.fr/strip/. ..