Gene Symbol: GDI1
Description: Gdi1p
Alias: SEC19, Gdi1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  2. Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, et al. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase. Science. 2003;302:646-50 pubmed
    ..Analysis of the structure provides a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans. ..
  3. Pylypenko O, Rak A, Durek T, Kushnir S, Dursina B, Thomae N, et al. Structure of doubly prenylated Ypt1:GDI complex and the mechanism of GDI-mediated Rab recycling. EMBO J. 2006;25:13-23 pubmed
    ..Based on structural information and biophysical measurements, we propose mechanistic and thermodynamic models for GDI and Rab escort protein-mediated interaction of RabGTPase with intracellular membranes. ..
  4. Collins R, Brennwald P, Garrett M, Lauring A, Novick P. Interactions of nucleotide release factor Dss4p with Sec4p in the post-Golgi secretory pathway of yeast. J Biol Chem. 1997;272:18281-9 pubmed
    ..We examined the relationship of GDI1 and DSS4 with SEC4 both genetically and biochemically...
  5. Finger F, Novick P. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:647-62 pubmed
    ..This suggests that SEC gene products are involved in determining the bud site and is consistent with a role for Sec3p in determining the correct site of exocytosis. ..
  6. Chen C, Collins R. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Biochem Soc Trans. 2005;33:614-8 pubmed
    ..e. independent or dependent activities of Yip1p. ..
  7. Alory C, Balch W. Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily. Mol Biol Cell. 2003;14:3857-67 pubmed
    ..We have now isolated REP mutant proteins that are able to partially function as both REP and GDI. These results provide molecular insight into the functional and evolutionary organization of the REP/GDI superfamily. ..
  8. Garrett M, Zahner J, Cheney C, Novick P. GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway. EMBO J. 1994;13:1718-28 pubmed
    ..Analogous to the bovine protein, purified Gdi1p slows the dissociation of GDP from Sec4p and releases the GDP-bound form from yeast membranes...
  9. Antebi A, Fink G. The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell. 1992;3:633-54 pubmed
    ..Some of these interactions are modulated by changes in external Ca2+ concentrations. These results imply a global role for Ca2+ in the proper function of components governing transit and processing through the secretory pathway. ..

More Information


  1. Calero M, Collins R. Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. Biochem Biophys Res Commun. 2002;290:676-81 pubmed
    ..The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. ..
  2. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  3. Luan P, Balch W, Emr S, Burd C. Molecular dissection of guanine nucleotide dissociation inhibitor function in vivo. Rab-independent binding to membranes and role of Rab recycling factors. J Biol Chem. 1999;274:14806-17 pubmed
    ..Furthermore, we find that membrane association of Gdi1p is Rab-independent...
  4. Ignatev A, Kravchenko S, Rak A, Goody R, Pylypenko O. A structural model of the GDP dissociation inhibitor rab membrane extraction mechanism. J Biol Chem. 2008;283:18377-84 pubmed publisher
    ..We suggest a model for the interaction of GDI with prenylated Rab proteins that incorporates a stepwise increase in affinity as the three different partial interactions are successively formed. ..
  5. Gilbert P, Burd C. GDP dissociation inhibitor domain II required for Rab GTPase recycling. J Biol Chem. 2001;276:8014-20 pubmed
    ..In this study, Domain II of yeast GDI, encoded by the essential GDI1/SEC19 gene, was targeted in a genetic screen to obtain mutants that might lend insight into the function of this domain...
  6. Cabrera M, Ungermann C. Guanine nucleotide exchange factors (GEFs) have a critical but not exclusive role in organelle localization of Rab GTPases. J Biol Chem. 2013;288:28704-12 pubmed publisher
    ..Our data agree with the emerging model that GEFs are critical for Rab localization but raise the possibility that additional factors can contribute to this process...
  7. Heger C, Wrann C, Collins R. Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. PLoS ONE. 2011;6:e24332 pubmed publisher
  8. Ezaki B, Nakakihara E. Possible involvement of GDI1 protein, a GDP dissociation inhibitor related to vesicle transport, in an amelioration of zinc toxicity in Saccharomyces cerevisiae. Yeast. 2012;29:17-24 pubmed publisher
    ..A temperature-sensitive gdi1 mutant (originally called sec19), in which the GDP dissociation inhibitor becomes inactive at the non-permissive temperature (37?°C), was more ..
  9. Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
  10. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  11. Ignatev A, Piatkov K, Pylypenko O, Rak A. A size filtration approach to purify low affinity complexes for crystallization. J Struct Biol. 2007;159:154-7 pubmed
    ..The resulting model of the low affinity unprenylated Rab-GDI complex should reflect a transient Rab-GDI complex when GDI is bound to the membrane-anchored Rab protein and is poised to extract Rab to cytosol. ..