Genomes and Genes
Gene Symbol: GCS1
Species: Saccharomyces cerevisiae S288c
- Wong T, Fairn G, Poon P, Shmulevitz M, McMaster C, Singer R, et al. Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proc Natl Acad Sci U S A. 2005;102:12777-82 pubmed..cerevisiae contains a family of Arf (ADP-ribosylation factor) GTPase activating protein (GAP) proteins with the Gcs1 + Age2 ArfGAP pair providing essential overlapping function for the movement of transport vesicles from the trans-..
- Zhang C, Bowzard J, Greene M, Anido A, Stearns K, Kahn R. Genetic interactions link ARF1, YPT31/32 and TRS130. Yeast. 2002;19:1075-86 pubmed..defects in several alleles of ARF1, increasing the gene dosage of YPT31/32 suppressed the cold sensitivity of gcs1(-), an Arf GTPase-activating protein (GAP)...
- Drebot M, Johnston G, Singer R. A yeast mutant conditionally defective only for reentry into the mitotic cell cycle from stationary phase. Proc Natl Acad Sci U S A. 1987;84:7948-52 pubmed..showed that the mutant phenotype is due to the interaction between two mutations, a cold-sensitive mutation gcs1 and a suppressor mutation sed1...
- Poon P, Wang X, Rotman M, Huber I, Cukierman E, Cassel D, et al. Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc Natl Acad Sci U S A. 1996;93:10074-7 pubmed..A previously identified yeast protein, Gcs1, exhibits structural similarity to a mammalian protein with ARF-GAP activity in vitro...
- Zhang C, Cavenagh M, Kahn R. A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae. J Biol Chem. 1998;273:19792-6 pubmed..Included in this family is Gcs1, a protein previously described (Poon, P. P., Wang, X., Rotman, M., Huber, I., Cukierman, E., Cassel, D., Singer, R...
- Yanagisawa L, Marchena J, Xie Z, Li X, Poon P, Singer R, et al. Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast. Mol Biol Cell. 2002;13:2193-206 pubmed..We now report genetic evidence to suggest that two ADP ribosylation factor-GTPase-activating proteins (ARFGAPs), Gcs1p and Age2p, may represent these lipid-responsive elements, and that Gcs1p/Age2p act downstream of Sec14p and ..
- Suckling R, Poon P, Travis S, Majoul I, Hughson F, Evans P, et al. Structural basis for the binding of tryptophan-based motifs by Î´-COP. Proc Natl Acad Sci U S A. 2015;112:14242-7 pubmed publisher..We also show that the Saccharomyces cerevisiae Arf GTPase-activating protein (GAP) homolog Gcs1p uses a related WxxF motif at its extreme C terminus to bind to Î´-COP at the same site in the same way...
- Zhang C, Bowzard J, Anido A, Kahn R. Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions. Yeast. 2003;20:315-30 pubmed..The description of the specificities of the ARF GAPs for the different responses is viewed as a necessary first step in dissecting biologically relevant pathways through a functionally overlapping family of signalling proteins. ..
- Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..
- Chen K, Tsai P, Liu Y, Lee F. Competition between the golgin Imh1p and the GAP Gcs1p stabilizes activated Arl1p at the late-Golgi. J Cell Sci. 2012;125:4586-96 pubmed publisher..Biochemically, purified Imh1p competes with Gcs1p, an Arl1p GTPase-activating protein (GAP), for binding to Arl1p, thus interfering with the GAP activity of Gcs1p ..
- Eugster A, Frigerio G, Dale M, Duden R. COP I domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP. EMBO J. 2000;19:3905-17 pubmed..subunits with regulatory proteins: beta'- and gamma-COP interact with the ARF-GTP-activating protein (GAP) Glo3p, but not Gcs1p, and beta- and epsilon-COP interact with ARF-GTP. Glo3p also interacts with intact coatomer in vitro.
- Schindler C, Spang A. Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF. Mol Biol Cell. 2007;18:2852-63 pubmed..Here, we show that the ArfGAP Gcs1p accelerates vesicle (v)-target membrane (t)-SNARE complex formation in vitro, indicating that ArfGAPs may act as ..
- Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
- Benjamin J, Poon P, Drysdale J, Wang X, Singer R, Johnston G. Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast. Mol Biol Cell. 2011;22:2337-47 pubmed publisher..The yeast Gcs1 protein is a dual-specificity GAP for ADP-ribosylation factor (Arf) and Arf-like (Arl)1 G proteins, and also has ..
- Hsu J, Chen Z, Liu Y, Lee F. Mechanism of action of the flippase Drs2p in modulating GTP hydrolysis of Arl1p. J Cell Sci. 2014;127:2615-20 pubmed publisher..The GTP-hydrolysis activity of Arl1p is stimulated by its GTPase-activating protein Gcs1p, and binding with its effector Imh1p protects Arl1p from premature inactivation...
- Yahara N, Sato K, Nakano A. The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J Cell Sci. 2006;119:2604-12 pubmed..By domain swapping analysis of Glo3p and another ArfGAP, Gcs1p, we have shown that the non-catalytic C-terminal region of Glo3p is required for the suppression of the growth ..
- Connolly J, Engebrecht J. The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p. Eukaryot Cell. 2006;5:112-24 pubmed..An examination of Gcs1p-green fluorescent protein revealed that it is a soluble protein...
- Filipak M, Drebot M, Ireland L, Singer R, Johnston G. Mitochondrial DNA loss by yeast reentry-mutant cells conditionally unable to proliferate from stationary phase. Curr Genet. 1992;22:471-7 pubmedDouble-mutant cells of the budding yeast Saccharomyces cerevisiae harboring the gcs1-1 and sed1-1 mutations are conditionally defective (cold-sensitive) only for reentry into the mitotic cycle from stationary phase...
- Zendeh Boodi Z, Yamamoto T, Sakane H, Tanaka K. Identification of a second amphipathic lipid-packing sensor-like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway. J Biochem. 2013;153:573-87 pubmed publisher..sensor (ALPS) motif was first identified in the C-terminal regions of ArfGAP1 and its yeast homologue Gcs1p as a region that adsorbs preferentially onto highly curved membranes by folding into an amphipathic ?-helix (AH)...
- Li X, Rivas M, Fang M, Marchena J, Mehrotra B, Chaudhary A, et al. Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex. J Cell Biol. 2002;157:63-77 pubmed
- Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5021-37 pubmed..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER. ..
- Blader I, Cope M, Jackson T, Profit A, Greenwood A, Drubin D, et al. GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. Mol Biol Cell. 1999;10:581-96 pubmed..In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene...