GCN5

Summary

Gene Symbol: GCN5
Description: histone acetyltransferase GCN5
Alias: AAS104, ADA4, KAT2, SWI9, histone acetyltransferase GCN5
Species: Saccharomyces cerevisiae S288c
Products:     GCN5

Top Publications

  1. Hassan A, Awad S, Al Natour Z, Othman S, Mustafa F, Rizvi T. Selective recognition of acetylated histones by bromodomains in transcriptional co-activators. Biochem J. 2007;402:125-33 pubmed
    ..Our results reveal that the Swi2/Snf2 bromodomain interacts with various acetylated H3 and H4 peptides, whereas the Gcn5 bromodomain interacts only with acetylated H3 peptides and tetra-acetylated H4 peptides...
  2. Burgess R, Zhou H, Han J, Zhang Z. A role for Gcn5 in replication-coupled nucleosome assembly. Mol Cell. 2010;37:469-80 pubmed publisher
    ..Here we show that Gcn5, a KAT that functions in transcription, works in parallel with Rtt109, the H3 lysine 56 KAT, to promote RC ..
  3. Lee K, Florens L, Swanson S, Washburn M, Workman J. The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex. Mol Cell Biol. 2005;25:1173-82 pubmed
    ..Taken together, these data indicate that the expression of some genes, including ARG1, is regulated by a balance of histone H2B ubiquitylation in the cell. ..
  4. Syntichaki P, Thireos G. The Gcn5.Ada complex potentiates the histone acetyltransferase activity of Gcn5. J Biol Chem. 1998;273:24414-9 pubmed
    The Gcn5 histone acetyltransferase (HAT) is part of a large multimeric complex that is required for transcriptional activation in yeast...
  5. Powell D, Weaver C, Jennings J, McAfee K, He Y, Weil P, et al. Cluster analysis of mass spectrometry data reveals a novel component of SAGA. Mol Cell Biol. 2004;24:7249-59 pubmed
    ..Our data suggest that the role of SGF11 in transcription is independent of SAGA's histone acetyltransferase activity but may involve Ubp8p recruitment to or stabilization in SAGA. ..
  6. Imoberdorf R, Topalidou I, Strubin M. A role for gcn5-mediated global histone acetylation in transcriptional regulation. Mol Cell Biol. 2006;26:1610-6 pubmed
    ..However, in addition to being targeted to specific loci, HATs such as Gcn5 also modify histones genome-wide...
  7. Horiuchi J, Silverman N, Pina B, Marcus G, Guarente L. ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3. Mol Cell Biol. 1997;17:3220-8 pubmed
    ..Furthermore, ADA1 interacts with the other ADAs in the ADA/GCN5 complex as demonstrated by partial purification of the complex and immunoprecipitation experiments...
  8. Larschan E, Winston F. The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev. 2001;15:1946-56 pubmed
    Previous studies demonstrated that the SAGA (Spt-Ada-Gcn5-Acetyltransferase) complex facilitates the binding of TATA-binding protein (TBP) during transcriptional activation of the GAL1 gene of Saccharomyces cerevisiae...
  9. Kuo M, Brownell J, Sobel R, Ranalli T, Cook R, Edmondson D, et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature. 1996;383:269-72 pubmed

More Information

Publications76

  1. Roberts S, Winston F. Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes. Genetics. 1997;147:451-65 pubmed
    ..A related study demonstrates that this complex also contains the histone acetyltransferase, Gcn5, and Ada2. This complex has been named SAGA (Spt/Ada/Gcn5 acetyltransferase)...
  2. Howe L, Kusch T, Muster N, Chaterji R, Yates J, Workman J. Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Mol Cell Biol. 2002;22:5047-53 pubmed
  3. Wyce A, Xiao T, Whelan K, Kosman C, Walter W, Eick D, et al. H2B ubiquitylation acts as a barrier to Ctk1 nucleosomal recruitment prior to removal by Ubp8 within a SAGA-related complex. Mol Cell. 2007;27:275-88 pubmed
    ..These findings reveal a mechanism by which H2B ubiquitylation acts as a barrier to Ctk1 association with active genes, while subsequent deubiquitylation by Ubp8 triggers Ctk1 recruitment at the appropriate point in activation. ..
  4. Rosaleny L, Ruiz García A, García Martínez J, Pérez Ortín J, Tordera V. The Sas3p and Gcn5p histone acetyltransferases are recruited to similar genes. Genome Biol. 2007;8:R119 pubmed
    ..Finally, a positive correlation between the decrease of H3 Lys14 acetylation in a GCN5 deleted strain and the Gcn5p genome occupancy is shown...
  5. Pollard K, Peterson C. Role for ADA/GCN5 products in antagonizing chromatin-mediated transcriptional repression. Mol Cell Biol. 1997;17:6212-22 pubmed
    ..We show here that mutations in ADA2, ADA3, and GCN5, which are believed to encode subunits of a nuclear histone acetyltransferase complex, cause phenotypes strikingly ..
  6. Pray Grant M, Schieltz D, McMahon S, Wood J, Kennedy E, Cook R, et al. The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol. 2002;22:8774-86 pubmed
    ..SAGA contains a number of subunits known to function in transcription including Spt and Ada proteins, the Gcn5 acetyltransferase, a subset of TATA-binding-protein-associated factors (TAF(II)s), and Tra1...
  7. Grant P, Eberharter A, John S, Cook R, Turner B, Workman J. Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem. 1999;274:5895-900 pubmed
    The coactivator/adaptor protein Gcn5 is a conserved histone acetyltransferase, which functions as the catalytic subunit in multiple yeast transcriptional regulatory complexes...
  8. Liu Y, Xu X, Kuo M. Snf1p regulates Gcn5p transcriptional activity by antagonizing Spt3p. Genetics. 2010;184:91-105 pubmed publisher
    ..Gcn5p function is to antagonize Spt3p, because the HIS3 expression defects caused by snf1 knockout, or by the TSTY gcn5 mutations, can be suppressed by deleting SPT3...
  9. Lee K, Swanson S, Florens L, Washburn M, Workman J. Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Epigenetics Chromatin. 2009;2:2 pubmed publisher
  10. Hoke S, Genereaux J, Liang G, Brandl C. A conserved central region of yeast Ada2 regulates the histone acetyltransferase activity of Gcn5 and interacts with phospholipids. J Mol Biol. 2008;384:743-55 pubmed publisher
    The SAGA (Spt-Ada-Gcn5 acetyltransferase) complex of Saccharomyces cerevisiae contains more than 20 components that acetylate and deubiquitylate nucleosomal histones...
  11. Marcus G, Silverman N, Berger S, Horiuchi J, Guarente L. Functional similarity and physical association between GCN5 and ADA2: putative transcriptional adaptors. EMBO J. 1994;13:4807-15 pubmed
    ..Here we identify two new genes by the same selection, one of which is identical to GCN5. We show that gcn5 mutants share properties with ada mutants, including slow growth, temperature sensitivity and ..
  12. Adkins M, Carson J, English C, Ramey C, Tyler J. The histone chaperone anti-silencing function 1 stimulates the acetylation of newly synthesized histone H3 in S-phase. J Biol Chem. 2007;282:1334-40 pubmed
    ..We show that Gcn5 is the histone acetyltransferase responsible for the S-phase-specific peak of H3 lysine 9 acetylation...
  13. Keogh M, Mennella T, Sawa C, Berthelet S, Krogan N, Wolek A, et al. The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4. Genes Dev. 2006;20:660-5 pubmed
    ..Function-specific modifications may help explain how the same component of chromatin can function in diverse pathways. ..
  14. Daniel J, Torok M, Sun Z, Schieltz D, Allis C, Yates J, et al. Deubiquitination of histone H2B by a yeast acetyltransferase complex regulates transcription. J Biol Chem. 2004;279:1867-71 pubmed
    ..we show that a putative deubiquitinating enzyme, Ubp8, is a structurally nonessential component of both the Spt-Ada-Gcn5-acetyltransferase (SAGA) and SAGA-like (SLIK) histone acetyltransferase (HAT) complexes in yeast...
  15. van Oevelen C, van Teeffelen H, Timmers H. Differential requirement of SAGA subunits for Mot1p and Taf1p recruitment in gene activation. Mol Cell Biol. 2005;25:4863-72 pubmed
    ..The deletion of either SPT3 or SPT8 reduces Mot1p binding to HXT2 and HXT4. Surprisingly, the deletion of GCN5 reduces Taf1p binding to both promoters...
  16. Wittschieben B, Fellows J, Du W, Stillman D, Svejstrup J. Overlapping roles for the histone acetyltransferase activities of SAGA and elongator in vivo. EMBO J. 2000;19:3060-8 pubmed
    Elp3 and Gcn5 are histone acetyltransferases (HATs) that function in transcription as subunits of Elongator and SAGA/ADA, respectively...
  17. Sudarsanam P, Cao Y, Wu L, Laurent B, Winston F. The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5. EMBO J. 1999;18:3101-6 pubmed
    ..Finally, Snf/Swi and Gcn5, a histone acetyltransferase, have partially redundant roles in the control of SUC2 transcription, suggesting a ..
  18. Candau R, Berger S. Structural and functional analysis of yeast putative adaptors. Evidence for an adaptor complex in vivo. J Biol Chem. 1996;271:5237-45 pubmed
    ..To study their functional interactions, deletion mutations in the yeast adaptors ADA2, GCN5, and ADA3 were created. We defined a region within the middle of GCN5 required for interaction with ADA2 in vitro...
  19. Clarke A, Lowell J, Jacobson S, Pillus L. Esa1p is an essential histone acetyltransferase required for cell cycle progression. Mol Cell Biol. 1999;19:2515-26 pubmed
    ..These observations therefore link an essential HAT activity to cell cycle progression, potentially through discrete transcriptional regulatory events. ..
  20. Xue Franzén Y, Johnsson A, Brodin D, Henriksson J, Burglin T, Wright A. Genome-wide characterisation of the Gcn5 histone acetyltransferase in budding yeast during stress adaptation reveals evolutionarily conserved and diverged roles. BMC Genomics. 2010;11:200 pubmed publisher
    b>Gcn5 is a transcriptional coactivator with histone acetyltransferase activity that is conserved with regard to structure as well as its histone substrates throughout the eukaryotes...
  21. Candau R, Zhou J, Allis C, Berger S. Histone acetyltransferase activity and interaction with ADA2 are critical for GCN5 function in vivo. EMBO J. 1997;16:555-65 pubmed
    Yeast GCN5 is one component of a putative adaptor complex that includes ADA2 and ADA3 and functionally connects DNA-bound transcriptional activators with general transcription factors...
  22. Pray Grant M, Daniel J, Schieltz D, Yates J, Grant P. Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. Nature. 2005;433:434-8 pubmed
    ..Yeast SAGA (Spt-Ada-Gcn5 acetyltransferase) and SLIK (SAGA-like) are two highly homologous and conserved multi-subunit HAT complexes, which ..
  23. Choi J, Grimes D, Rowe K, Howe L. Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylation. Mol Cell Biol. 2008;28:6967-72 pubmed publisher
    ..These results demonstrate for the first time the vital and yet redundant functions of histone H3 and Rsc4p acetylation in maintaining cell viability. ..
  24. Vernarecci S, Ornaghi P, Bâgu A, Cundari E, Ballario P, Filetici P. Gcn5p plays an important role in centromere kinetochore function in budding yeast. Mol Cell Biol. 2008;28:988-96 pubmed
    ..and the kinetochore, a protein complex hierarchically assembled in the centromeric DNA region, while disruption of GCN5 in mutants of inner components results in sick phenotype...
  25. Candau R, Moore P, Wang L, Barlev N, Ying C, Rosen C, et al. Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5. Mol Cell Biol. 1996;16:593-602 pubmed
    ..Consistent with this view, we have identified possible human homologs of yeast ADA2 (yADA2) and yeast GCN5 (yGCN5), components of a putative adaptor complex...
  26. Ricci A, Genereaux J, Brandl C. Components of the SAGA histone acetyltransferase complex are required for repressed transcription of ARG1 in rich medium. Mol Cell Biol. 2002;22:4033-42 pubmed
    ..Using gcn5 deletion strains and a Gcn5 protein carrying the E173Q mutation in the histone acetyltransferase (HAT) region, we ..
  27. Zhang W, Bone J, Edmondson D, Turner B, Roth S. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J. 1998;17:3155-67 pubmed
    ..of histones H3 and H4 and examined the effects of these mutations in yeast strains with and without functional GCN5. We found that in vivo, GCN5 is required either directly or indirectly for the acetylation of several sites in H3 ..
  28. Kasten M, Szerlong H, Erdjument Bromage H, Tempst P, Werner M, Cairns B. Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14. EMBO J. 2004;23:1348-59 pubmed
    ..Replacements involving Lys14 of histone H3 (the main target of Gcn5), but not other H3 or H4 lysine residues, also conferred severe growth defects to rsc4 mutant strains...
  29. VanDemark A, Blanksma M, Ferris E, Heroux A, Hill C, Formosa T. The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol Cell. 2006;22:363-74 pubmed
    ..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. ..
  30. Cairns B, Schlichter A, Erdjument Bromage H, Tempst P, Kornberg R, Winston F. Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. Mol Cell. 1999;4:715-23 pubmed
    ..Therefore, these domains are required for RSC function. Additional genetic analysis provides further evidence that RSC function is related to transcriptional control. ..
  31. Wang L, Mizzen C, Ying C, Candau R, Barlev N, Brownell J, et al. Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activation. Mol Cell Biol. 1997;17:519-27 pubmed
    Yeast and human ADA2 and GCN5 (y- and hADA2 and y- and hGCN5, respectively) have been shown to potentiate transcription in vivo and may function as adaptors to bridge physical interactions between DNA-bound activators and the basal ..
  32. Babiarz J, Halley J, Rine J. Telomeric heterochromatin boundaries require NuA4-dependent acetylation of histone variant H2A.Z in Saccharomyces cerevisiae. Genes Dev. 2006;20:700-10 pubmed
    ..Taken together, these results show a role for H2A.Z acetylation in restricting silent chromatin, and reveal that acetylation of H2A.Z and H4 can contribute to a common function essential to life. ..
  33. Horiuchi J, Silverman N, Marcus G, Guarente L. ADA3, a putative transcriptional adaptor, consists of two separable domains and interacts with ADA2 and GCN5 in a trimeric complex. Mol Cell Biol. 1995;15:1203-9 pubmed
    Mutations in yeast ADA2, ADA3, and GCN5 weaken the activation potential of a subset of acidic activation domains...
  34. Choy J, Kron S. NuA4 subunit Yng2 function in intra-S-phase DNA damage response. Mol Cell Biol. 2002;22:8215-25 pubmed
    ..In turn, mutants lacking the histone H3-specific acetyltransferase GCN5 are similarly sensitive to intra-S-phase DNA damage...
  35. Grant P, Duggan L, Cote J, Roberts S, Brownell J, Candau R, et al. Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex. Genes Dev. 1997;11:1640-50 pubmed
    The transcriptional adaptor protein Gcn5 has been identified as a nuclear histone acetyltransferase (HAT)...
  36. Balasubramanian R, Pray Grant M, Selleck W, Grant P, Tan S. Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylation. J Biol Chem. 2002;277:7989-95 pubmed
    Previous studies have shown that the transcriptional coactivator protein Gcn5 functions as a catalytic histone acetyltransferase (HAT)...
  37. Howe L, Auston D, Grant P, John S, Cook R, Workman J, et al. Histone H3 specific acetyltransferases are essential for cell cycle progression. Genes Dev. 2001;15:3144-54 pubmed
    ..Simultaneous disruption of SAS3, the homolog of the MOZ leukemia gene, and GCN5, the hGCN5/PCAF homolog, is synthetically lethal due to loss of acetyltransferase activity...
  38. Eberharter A, Sterner D, Schieltz D, Hassan A, Yates J, Berger S, et al. The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:6621-31 pubmed
    We have identified two Gcn5-dependent histone acetyltransferase (HAT) complexes from Saccharomyces cerevisiae, the 0.8-MDa ADA complex and the 1.8-MDa SAGA complex...
  39. Turner E, Malo M, Pisclevich M, Dash M, Davies G, Arnason T, et al. The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression. Eukaryot Cell. 2010;9:1418-31 pubmed publisher
    ..HDAC) mutants that genetically interact with the apc5(CA) (chromatin assembly) mutant, we found that deletion of GCN5 or ELP3 severely hampered apc5(CA) temperature-sensitive (ts) growth...
  40. Formosa T, Ruone S, Adams M, Olsen A, Eriksson P, Yu Y, et al. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Genetics. 2002;162:1557-71 pubmed
    ..Mutations that impair the reassembly activity cause chromatin to accumulate in an abnormally disrupted state, imposing a requirement for a nucleosome reassembly function that we propose is provided by Hir/Hpc proteins. ..
  41. Sklenar A, Parthun M. Characterization of yeast histone H3-specific type B histone acetyltransferases identifies an ADA2-independent Gcn5p activity. BMC Biochem. 2004;5:11 pubmed
    ..Deletion of the GCN5 and ADA3 genes resulted in the loss of HatB3.1 activity while deletion of ADA2 had no effect...
  42. Emre N, Ingvarsdottir K, Wyce A, Wood A, Krogan N, Henry K, et al. Maintenance of low histone ubiquitylation by Ubp10 correlates with telomere-proximal Sir2 association and gene silencing. Mol Cell. 2005;17:585-94 pubmed
    ..Our results suggest that these H2B-deubiquitylating enzymes have distinct genomic functions. ..
  43. Sterner D, Grant P, Roberts S, Duggan L, Belotserkovskaya R, Pacella L, et al. Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol. 1999;19:86-98 pubmed
    ..Specifically, null mutations in the Gcn5/Ada2/Ada3 or Spt3/Spt8 classes cause moderate phenotypes and subtle structural alterations, while mutations in a ..
  44. Burgess S, Ajimura M, Kleckner N. GCN5-dependent histone H3 acetylation and RPD3-dependent histone H4 deacetylation have distinct, opposing effects on IME2 transcription, during meiosis and during vegetative growth, in budding yeast. Proc Natl Acad Sci U S A. 1999;96:6835-40 pubmed
    ..IME1 is a positive activator of IME2, which activates downstream genes. We report that Gcn5, a histone H3 acetylase, plays a central role in initiation of meiosis via effects on IME2 expression...
  45. Hassan A, Prochasson P, Neely K, Galasinski S, Chandy M, Carrozza M, et al. Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes. Cell. 2002;111:369-79 pubmed
    ..by SWI/SNF and SAGA in the absence of transcription activators requires the bromodomains of the Swi2/Snf2 and Gcn5 subunits, respectively, and nucleosome acetylation...
  46. Wu P, Winston F. Analysis of Spt7 function in the Saccharomyces cerevisiae SAGA coactivator complex. Mol Cell Biol. 2002;22:5367-79 pubmed
    ..Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGA(alt)/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions. ..
  47. Sterner D, Belotserkovskaya R, Berger S. SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proc Natl Acad Sci U S A. 2002;99:11622-7 pubmed
    Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast...
  48. Orozco H, Matallana E, Aranda A. Wine yeast sirtuins and Gcn5p control aging and metabolism in a natural growth medium. Mech Ageing Dev. 2012;133:348-58 pubmed publisher
    ..Deletions of sirtuin HST2 and acetyltransferase GCN5 have the opposite effect to SIR2 mutation in both media...
  49. Brownell J, Zhou J, Ranalli T, Kobayashi R, Edmondson D, Roth S, et al. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation. Cell. 1996;84:843-51 pubmed
    ..This Tetrahymena enzyme is strikingly homologous to the yeast protein Gcn5, a putative transcriptional adaptor, and we demonstrate that recombinant Gcn5p possesses HAT activity...
  50. Sun Z, Hampsey M. A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae. Genetics. 1999;152:921-32 pubmed
    ..Strikingly, deletion of GCN5, which encodes a histone acetyltransferase, enhances silencing in a manner similar to deletion of RPD3...
  51. Han Q, Lu J, Duan J, Su D, Hou X, Li F, et al. Gcn5- and Elp3-induced histone H3 acetylation regulates hsp70 gene transcription in yeast. Biochem J. 2008;409:779-88 pubmed
    ..The HATs (histone acetyltransferases) Gcn5 (general control non-derepressible 5) and Elp3 (elongation protein 3) modulated hsp70 gene transcription by ..
  52. Liu Y, Xu X, Singh Rodriguez S, Zhao Y, Kuo M. Histone H3 Ser10 phosphorylation-independent function of Snf1 and Reg1 proteins rescues a gcn5- mutant in HIS3 expression. Mol Cell Biol. 2005;25:10566-79 pubmed
    b>Gcn5 protein is a prototypical histone acetyltransferase that controls transcription of multiple yeast genes...
  53. Wu P, Ruhlmann C, Winston F, Schultz P. Molecular architecture of the S. cerevisiae SAGA complex. Mol Cell. 2004;15:199-208 pubmed
    ..Three components that perform distinct regulatory functions, Spt3, Gcn5, and Tra1, are spatially separated, underscoring the modular nature of the complex...
  54. Johnsson A, Xue Franzén Y, Lundin M, Wright A. Stress-specific role of fission yeast Gcn5 histone acetyltransferase in programming a subset of stress response genes. Eukaryot Cell. 2006;5:1337-46 pubmed
    b>Gcn5 is a coactivator protein that contributes to gene activation by acetylating specific lysine residues within the N termini of histone proteins...
  55. Henry K, Wyce A, Lo W, Duggan L, Emre N, Kao C, et al. Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev. 2003;17:2648-63 pubmed
    ..lowered transcription of SAGA-regulated genes, and the severity of this defect was exacerbated by codeletion of the Gcn5 acetyltransferase within SAGA...
  56. Barbaric S, Reinke H, Hörz W. Multiple mechanistically distinct functions of SAGA at the PHO5 promoter. Mol Cell Biol. 2003;23:3468-76 pubmed
    ..rate of chromatin remodeling and consequently the rate of PHO5 activation are strongly decreased in the absence of Gcn5 histone acetyltransferase activity...
  57. Pascual García P, Govind C, Queralt E, Cuenca Bono B, Llopis A, Chávez S, et al. Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2. Genes Dev. 2008;22:2811-22 pubmed publisher
    ..Our results reveal that Sus1 plays a key role in coordinating gene transcription and mRNA export by working at the interface between the SAGA and TREX2 complexes during transcription elongation. ..
  58. Köhler A, Zimmerman E, Schneider M, Hurt E, Zheng N. Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module. Cell. 2010;141:606-17 pubmed publisher
    ..Our structural and functional analyses reveal a central role of Sgf11 and Sgf73 in activating Ubp8 for deubiquitinating histone H2B and demonstrate how a DUB can be allosterically regulated by its nonsubstrate partners. ..
  59. Ruiz García A, Sendra R, Pamblanco M, Tordera V. Gcn5p is involved in the acetylation of histone H3 in nucleosomes. FEBS Lett. 1997;403:186-90 pubmed
    Enzymatic extracts from a gcn5 mutant and wild-type strains of Saccharomyces cerevisiae were chromatographically fractionated and the histone acetyltransferase activities compared...
  60. Grant P, Schieltz D, Pray Grant M, Steger D, Reese J, Yates J, et al. A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell. 1998;94:45-53 pubmed
    ..The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity...
  61. Grant P, Schieltz D, Pray Grant M, Yates J, Workman J. The ATM-related cofactor Tra1 is a component of the purified SAGA complex. Mol Cell. 1998;2:863-7 pubmed
    ..These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter. ..
  62. Sermwittayawong D, Tan S. SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment. EMBO J. 2006;25:3791-800 pubmed
    In yeast, the multisubunit SAGA (Spt-Ada-Gcn5-acetyltransferase) complex acts as a coactivator to recruit the TATA-binding protein (TBP) to the TATA box, a critical step in eukaryotic gene regulation...
  63. Köhler A, Schneider M, Cabal G, Nehrbass U, Hurt E. Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat Cell Biol. 2008;10:707-15 pubmed publisher
    ..Thus, Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA. ..
  64. Krebs J, Fry C, Samuels M, Peterson C. Global role for chromatin remodeling enzymes in mitotic gene expression. Cell. 2000;102:587-98 pubmed
    ..We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin. ..
  65. VanDemark A, Kasten M, Ferris E, Heroux A, Hill C, Cairns B. Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation. Mol Cell. 2007;27:817-28 pubmed
    ..Endogenous Rsc4 is acetylated only at K25, and Gcn5 is identified as necessary and sufficient for Rsc4 K25 acetylation in vivo and in vitro...
  66. Papamichos Chronakis M, Petrakis T, Ktistaki E, Topalidou I, Tzamarias D. Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1. Mol Cell. 2002;9:1297-305 pubmed
    ..At the GAL1 promoter, Cyc8-Tup1 facilitates recruitment of SAGA (Spt-Ada-Gcn5-acetyltranferase) via Cti6, a PHD domain protein that physically links the Cyc8-Tup1 and SAGA complexes...
  67. Govind C, Zhang F, Qiu H, Hofmeyer K, Hinnebusch A. Gcn5 promotes acetylation, eviction, and methylation of nucleosomes in transcribed coding regions. Mol Cell. 2007;25:31-42 pubmed
    ..Gcn5p also enhances H3-K4 trimethylation in the ARG1 ORF and bulk histones. Thus, Gcn5p, most likely in SAGA, stimulates modification and eviction of nucleosomes in transcribed coding sequences and promotes Pol II elongation. ..