GCD6

Summary

Gene Symbol: GCD6
Description: Gcd6p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genes Dev 12:514-26. 1998
  2. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
  3. pmc Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation
    Edith Gomez
    Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, UK
    EMBO J 21:5292-301. 2002
  4. pmc Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:1920-32. 1993
  5. pmc Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:4618-31. 1993
  6. pmc Modulation of tRNA(iMet), eIF-2, and eIF-2B expression shows that GCN4 translation is inversely coupled to the level of eIF-2.GTP.Met-tRNA(iMet) ternary complexes
    T E Dever
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 2785, USA
    Mol Cell Biol 15:6351-63. 1995
  7. pmc Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation
    E Gomez
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 20:3965-76. 2000
  8. pmc Critical contacts between the eukaryotic initiation factor 2B (eIF2B) catalytic domain and both eIF2beta and -2gamma mediate guanine nucleotide exchange
    Sarah S Mohammad-Qureshi
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, United Kingdom
    Mol Cell Biol 27:5225-34. 2007
  9. ncbi Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae
    J Nika
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080, USA
    J Biol Chem 275:26011-7. 2000
  10. pmc Identification of intersubunit domain interactions within eukaryotic initiation factor (eIF) 2B, the nucleotide exchange factor for translation initiation
    Peter J Reid
    Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 287:8275-85. 2012

Detail Information

Publications19

  1. pmc eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange
    G D Pavitt
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genes Dev 12:514-26. 1998
    ..In contrast, the GCD1 and GCD6 subunits form an eIF2B subcomplex that binds equally to eIF2 and eIF2(alphaP)...
  2. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
    ..While eIF2B contains five subunits, the ε/Gcd6 subunit is sufficient for GEF activity in vitro...
  3. pmc Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation
    Edith Gomez
    Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, UK
    EMBO J 21:5292-301. 2002
    ..Here we report the results of a molecular dissection of the yeast epsilon subunit encoded by GCD6 in which we have identified the catalytic domain...
  4. pmc Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:1920-32. 1993
    ..We have isolated and characterized the GCD6 and GCD7 genes and shown that their products are required to repress GCN4 translation under nonstarvation ..
  5. pmc Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3
    J L Bushman
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 13:4618-31. 1993
    ..Genetic evidence that GCD6 and GCD7 encode additional subunits of the GCD-eIF-2B complex was provided by the fact that reduced-function ..
  6. pmc Modulation of tRNA(iMet), eIF-2, and eIF-2B expression shows that GCN4 translation is inversely coupled to the level of eIF-2.GTP.Met-tRNA(iMet) ternary complexes
    T E Dever
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 2785, USA
    Mol Cell Biol 15:6351-63. 1995
    ..Met-tRNA(iMet) ternary complexes is the cardinal parameter determining the site of reinitiation on GCN4 mRNA and support the idea that reinitiation at GCN4 is inversely related to the concentration of ternary complexes in the cell...
  7. pmc Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation
    E Gomez
    Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee, United Kingdom
    Mol Cell Biol 20:3965-76. 2000
    ..and in binding to eIF2 by characterizing mutations in the Saccharomyces cerevisiae gene encoding eIF2Bepsilon (GCD6) that impair the essential function of eIF2B...
  8. pmc Critical contacts between the eukaryotic initiation factor 2B (eIF2B) catalytic domain and both eIF2beta and -2gamma mediate guanine nucleotide exchange
    Sarah S Mohammad-Qureshi
    Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, United Kingdom
    Mol Cell Biol 27:5225-34. 2007
    ..In contrast, all three residues are critical for interaction with eIF2gamma. These data show that multiple contacts between eIF2gamma and eIF2Bepsilon mediate nucleotide exchange...
  9. ncbi Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae
    J Nika
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080, USA
    J Biol Chem 275:26011-7. 2000
    ..In support of this model, our yeast eIF2B preparations bind guanine nucleotides, with an apparent dissociation constant for GTP in the low micromolar range...
  10. pmc Identification of intersubunit domain interactions within eukaryotic initiation factor (eIF) 2B, the nucleotide exchange factor for translation initiation
    Peter J Reid
    Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 287:8275-85. 2012
    ..We have identified extensive interactions between the PLDs and left-handed β helix domains that form the eIF2Bγε subcomplex and propose a model for domain interactions between eIF2B subunits...
  11. pmc The yeast eukaryotic translation initiation factor 2B translation initiation complex interacts with the fatty acid synthesis enzyme YBR159W and endoplasmic reticulum membranes
    Christopher M Browne
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Mol Cell Biol 33:1041-56. 2013
    ..We show that a ybr159wΔ yeast strain and other strains with null mutations in the VLCFA pathway cause eIF2B to appear as numerous foci throughout the cytoplasm...
  12. pmc The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus
    Rachel Elsby
    Department of Cell Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    J Virol 85:9716-25. 2011
    ..Our data emphasize the importance of eIF2Bα in mediating the eIF2 kinase translation-inhibitory activity and may provide insight into the complex nature of viral oncolysis...
  13. pmc A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast
    A M Cigan
    Section on Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:5350-4. 1993
    ..We present biochemical evidence that five translational regulators of GCN4 encoded by GCD1, GCD2, GCD6, GCD7, and GCN3 are components of a protein complex that stably interacts with eIF-2 and represents the yeast ..
  14. doi The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε
    Zuoqi Gai
    Graduate School of Life Science, Faculty of Advanced Life Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochem Biophys Res Commun 423:515-9. 2012
    ..Such flexibility of eIF2β-NTD is expected to be responsible for its binding capability...
  15. ncbi Direct binding of translation initiation factor eIF2gamma-G domain to its GTPase-activating and GDP-GTP exchange factors eIF5 and eIF2B epsilon
    Pankaj V Alone
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:12636-44. 2006
    ....
  16. pmc An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation
    Chingakham Ranjit Singh
    Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, 66506, USA
    EMBO J 25:4537-46. 2006
    ..We propose that the eIF2/eIF5 complex represents a cytoplasmic reservoir for eIF2 that antagonizes eIF2B-promoted guanine nucleotide exchange, enabling coordinated regulation of translation initiation...
  17. doi Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics
    Keiji Kito
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan
    Proteomics 8:2366-70. 2008
    ..We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28...
  18. pmc Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2
    K Asano
    Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 18:1673-88. 1999
    ..Thus, the bipartite motif in eIF5 appears to be multifunctional, stimulating its recruitment to the 40S pre-initiation complex through interaction with eIF3 in addition to binding of its substrate eIF2...
  19. ncbi Identification and characterization of four new GCD genes in Saccharomyces cerevisiae
    P Niederberger
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Curr Genet 10:657-64. 1986
    ..including strain RH558 gcd2-1 isolated earlier, yielded five complementation groups: GCD2, GCD3, GCD4, GCD5, and GCD6. All mutant strains showed a dual phenotype, which was not separable by wild type backcrosses: "constitutive ..