Gene Symbol: GAT1
Description: Gat1p
Alias: MEP80, NIL1, Gat1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Coffman J, Rai R, Cunningham T, Svetlov V, Cooper T. Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:847-58 pubmed
    ..Here we demonstrate that another positive regulator, designated Gat1p, participates in the transcription of NCR-sensitive genes and is able to weakly activate transcription when ..
  2. Luzzani C, Cardillo S, Bermudez Moretti M, Correa Garcia S. New insights into the regulation of the Saccharomyces cerevisiae UGA4 gene: two parallel pathways participate in carbon-regulated transcription. Microbiology. 2007;153:3677-84 pubmed
    ..Furthermore, the results demonstrate the existence of a new factor operating in UGA4 regulation. ..
  3. Feller A, Georis I, Tate J, Cooper T, Dubois E. Alterations in the Ure2 ?Cap domain elicit different GATA factor responses to rapamycin treatment and nitrogen limitation. J Biol Chem. 2013;288:1841-55 pubmed publisher
    Ure2 is a phosphoprotein and central negative regulator of nitrogen-responsive Gln3/Gat1 localization and their ability to activate transcription...
  4. Beck T, Hall M. The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors. Nature. 1999;402:689-92 pubmed
    ..Thus, the TOR signalling pathway broadly controls nutrient metabolism by sequestering several transcription factors in the cytoplasm. ..
  5. Cunningham T, Andhare R, Cooper T. Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gat1p and Ure2p production in Saccharomyces cerevisiae. J Biol Chem. 2000;275:14408-14 pubmed
    GATA family activators (Gln3p and Gat1p) and repressors (Dal80p and Deh1p) regulate nitrogen catabolite repression (NCR)-sensitive transcription in Saccharomyces cerevisiae presumably via their competitive binding to the GATA sequences ..
  6. Stanbrough M, Rowen D, Magasanik B. Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes. Proc Natl Acad Sci U S A. 1995;92:9450-4 pubmed
    We have isolated the NIL1 gene, whose product is an activator of the transcription of nitrogen-regulated genes, by virtue of the homology of its zinc-finger domain to that of the previously identified activator, the product of GLN3...
  7. Georis I, Feller A, Vierendeels F, Dubois E. The yeast GATA factor Gat1 occupies a central position in nitrogen catabolite repression-sensitive gene activation. Mol Cell Biol. 2009;29:3803-15 pubmed publisher
    ..GATA factor, Gln3, was considered the major activator of NCR-sensitive gene expression, our work positions Gat1 as a key factor for the integrated control of NCR in yeast for the following reasons: (i) Gat1 appeared to be the ..
  8. Rai R, Tate J, Georis I, Dubois E, Cooper T. Constitutive and nitrogen catabolite repression-sensitive production of Gat1 isoforms. J Biol Chem. 2014;289:2918-33 pubmed publisher
    Nitrogen catabolite repression (NCR)-sensitive transcription is activated by Gln3 and Gat1. In nitrogen excess, Gln3 and Gat1 are cytoplasmic, and transcription is minimal...
  9. Georis I, Tate J, Cooper T, Dubois E. Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine. J Biol Chem. 2011;286:44897-912 pubmed publisher
    ..utilized nitrogen sources by governing the localization and function of transcription activators, Gln3 and Gat1. TorC1 inhibitor, rapamycin (Rap), and glutamine synthetase inhibitor, methionine sulfoximine (Msx), elicit ..

More Information


  1. Tate J, Georis I, Dubois E, Cooper T. Distinct phosphatase requirements and GATA factor responses to nitrogen catabolite repression and rapamycin treatment in Saccharomyces cerevisiae. J Biol Chem. 2010;285:17880-95 pubmed publisher
    ..TorC1 and intracellular nitrogen levels regulate the localization of Gln3 and Gat1, the activators of nitrogen catabolite repression (NCR)-sensitive genes whose products are required to utilize poor ..
  2. Georis I, Tate J, Cooper T, Dubois E. Tor pathway control of the nitrogen-responsive DAL5 gene bifurcates at the level of Gln3 and Gat1 regulation in Saccharomyces cerevisiae. J Biol Chem. 2008;283:8919-29 pubmed publisher
    ..gene expression that correlates with intracellular localization of GATA transcription activators Gln3 and Gat1/Nil1. Gln3-Myc(13) and Gat1-Myc(13) are restricted to the cytoplasm of cells provided with good nitrogen sources, e...
  3. Hong J, Gresham D. Molecular specificity, convergence and constraint shape adaptive evolution in nutrient-poor environments. PLoS Genet. 2014;10:e1004041 pubmed publisher
    ..observed the repeated independent selection of a multi-locus genotype, comprised of the functionally related genes GAT1, MEP2 and LST4...
  4. Lee K, Hahn J. Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae. Mol Microbiol. 2013;88:1120-34 pubmed publisher
    ..ARO9 and ARO10 are also under the control of nitrogen catabolite repression, but the direct roles for GATA factors, Gat1 and Gln3, in this regulation have not yet been elucidated...
  5. Soussi Boudekou S, Vissers S, Urrestarazu A, Jauniaux J, Andre B. Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol. 1997;23:1157-68 pubmed
    In Saccharomyces cerevisiae, two positive transcription factors of the GATA family, Gln3p and Nil1p/Gat1p, upregulate the expression of multiple nitrogen pathway genes via upstream 5'-GATA-3' sequences...
  6. Bernard A, Jin M, Xu Z, Klionsky D. A large-scale analysis of autophagy-related gene expression identifies new regulators of autophagy. Autophagy. 2015;11:2114-2122 pubmed publisher
    ..By providing a detailed analysis of the regulatory network of the ATG genes our study paves the way for future research on autophagy regulation and signaling. ..
  7. Oliveira E, Mansure J, Bon E. Gln3p and Nil1p regulation of invertase activity and SUC2 expression in Saccharomyces cerevisiae. FEMS Yeast Res. 2005;5:605-9 pubmed
    ..In the present work we showed that invertase levels displayed by the single nil1 and gln3 and by the double gln3nil1 mutant cells, cultivated in a sucrose-ammonium medium and collected at the ..
  8. Fayyadkazan M, Tate J, Vierendeels F, Cooper T, Dubois E, Georis I. Components of Golgi-to-vacuole trafficking are required for nitrogen- and TORC1-responsive regulation of the yeast GATA factors. Microbiologyopen. 2014;3:271-87 pubmed publisher
    ..Expression of NCR-sensitive genes is mediated by two transcription activators, Gln3 and Gat1, in response to provision of a poorly used nitrogen source or following treatment with the TORC1 inhibitor, ..
  9. Georis I, Isabelle G, Tate J, Vierendeels F, Cooper T, Dubois E. Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production. RNA Biol. 2015;12:824-37 pubmed publisher
    ..In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs ..
  10. Georis I, Feller A, Tate J, Cooper T, Dubois E. Nitrogen catabolite repression-sensitive transcription as a readout of Tor pathway regulation: the genetic background, reporter gene and GATA factor assayed determine the outcomes. Genetics. 2009;181:861-74 pubmed publisher
    ..Two GATA factors, Gln3 and Gat1, are responsible for NCR-sensitive transcription, but recent evidence demonstrates that Tor pathway regulation of ..
  11. Leverentz M, Campbell R, Connolly Y, Whetton A, Reece R. Mutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner. J Biol Chem. 2009;284:24115-22 pubmed publisher
    ..activation of the PUT genes requires both Put3p and the positively acting GATA factors, Gln3p and Gat1p. Conversely, the phosphorylation of Put3p is not dependent on GATA factor activity...
  12. Staschke K, Dey S, Zaborske J, Palam L, McClintick J, Pan T, et al. Integration of general amino acid control and target of rapamycin (TOR) regulatory pathways in nitrogen assimilation in yeast. J Biol Chem. 2010;285:16893-911 pubmed publisher
    ..This study highlights the mechanisms by which the GAAC and TOR pathways are integrated to recognize changing nitrogen availability and direct the transcriptome for optimal growth adaptation. ..
  13. Bertram P, Choi J, Carvalho J, Ai W, Zeng C, Chan T, et al. Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases. J Biol Chem. 2000;275:35727-33 pubmed
    ..In contrast to the free Gln3p, the Ure2p-bound Gln3p is signifcantly resistant to dephosphorylation. Taken together, these results reveal a tripartite regulatory mechanism by which the phosphorylation of Gln3p is regulated. ..
  14. Rubio Texeira M. Urmylation controls Nil1p and Gln3p-dependent expression of nitrogen-catabolite repressed genes in Saccharomyces cerevisiae. FEBS Lett. 2007;581:541-50 pubmed
    ..Altogether, the data presented here indicate an important role of the urmylation pathway in regulating the expression of genes involved in sensing and controlling amino acids levels. ..
  15. Coffman J, Rai R, Loprete D, Cunningham T, Svetlov V, Cooper T. Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J Bacteriol. 1997;179:3416-29 pubmed
    ..cerevisiae has been reported to be regulated by three GATA family proteins, the positive regulators Gln3p and Gat1p/Nil1p and the negative regulator Dal80p/Uga43p...
  16. Huang Y, Chen H, Teng S. Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast. RNA. 2010;16:2085-93 pubmed publisher
    ..Our results show for the first time that intragenic noncoding transcription promotes gene expression. ..
  17. Bianchi M, Costanzo G, Chelstowska A, Grabowska D, Mazzoni C, Piccinni E, et al. The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast. Mol Microbiol. 2004;53:953-68 pubmed
    ..suppressor genes, revealing that this phenotype can be suppressed by overdosing the transcription factors BDF1 and GAT1 in the yaf9Delta strain...
  18. Zhao X, Zou H, Chen J, Du G, Zhou J. The modification of Gat1p in nitrogen catabolite repression to enhance non-preferred nitrogen utilization in Saccharomyces cerevisiae. Sci Rep. 2016;6:21603 pubmed publisher
    ..Among these regulators, two positive regulators (Gln3p and Gat1p) could be phosphorylated and sequestered in the cytoplasm leading to the transcription of non-preferred nitrogen ..
  19. Crespo J, Daicho K, Ushimaru T, Hall M. The GATA transcription factors GLN3 and GAT1 link TOR to salt stress in Saccharomyces cerevisiae. J Biol Chem. 2001;276:34441-4 pubmed
    ..Second, the absence of the TOR-controlled GATA transcription factors GLN3 and GAT1 results in reduced basal and salt-induced expression of ENA1...
  20. Giannattasio S, Liu Z, Thornton J, Butow R. Retrograde response to mitochondrial dysfunction is separable from TOR1/2 regulation of retrograde gene expression. J Biol Chem. 2005;280:42528-35 pubmed
    ..DAL5 expression depends on the GATA transcription factors Gln3p and Gat1p. Gat1p is translocated to the nucleus only upon TOR inhibition by rapamycin...
  21. Marr N, Foglia J, Terebiznik M, Athenstaedt K, Zaremberg V. Controlling lipid fluxes at glycerol-3-phosphate acyltransferase step in yeast: unique contribution of Gat1p to oleic acid-induced lipid particle formation. J Biol Chem. 2012;287:10251-64 pubmed publisher
    ..Two isoforms, Gat1p/Gpt2p and Gat2p/Sct1p, are present in the yeast Saccharomyces cerevisiae...
  22. Ungar L, Harari Y, Toren A, Kupiec M. Tor complex 1 controls telomere length by affecting the level of Ku. Curr Biol. 2011;21:2115-20 pubmed publisher
    ..The TORC1 signal is transduced by the Gln3/Gat1/Ure2 pathway, which controls the levels of the Ku heterodimer, a telomere regulator...
  23. Soussi Boudekou S, Andre B. A co-activator of nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol. 1999;31:753-62 pubmed
    ..Ada1/Gan1p thus represents the first reported case of an accessory protein (a co-activator) linking the GATA-binding proteins Gln3p and Nil1p, mediating nitrogen-regulated transcription, to the basal transcription machinery. ..
  24. Fayyad Kazan M, Feller A, Bodo E, Boeckstaens M, Marini A, Dubois E, et al. Yeast nitrogen catabolite repression is sustained by signals distinct from glutamine and glutamate reservoirs. Mol Microbiol. 2016;99:360-79 pubmed publisher
    ..Providing additional evidence uncoupling TORC1 activity and NCR, our work revisits the dogmas underlying NCR regulation. ..
  25. Schmelzle T, Beck T, Martin D, Hall M. Activation of the RAS/cyclic AMP pathway suppresses a TOR deficiency in yeast. Mol Cell Biol. 2004;24:338-51 pubmed
    ..Our findings suggest that TOR signals through the RAS/cAMP pathway, independently of TAP42/SIT4. Therefore, the RAS/cAMP pathway may be a novel TOR effector branch. ..