Genomes and Genes
Gene Symbol: GAS1
Description: 1,3-beta-glucanosyltransferase GAS1
Alias: CWH52, GGP1, 1,3-beta-glucanosyltransferase GAS1
Species: Saccharomyces cerevisiae S288c
- Mouyna I, Fontaine T, Vai M, Monod M, Fonzi W, Diaquin M, et al. Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall. J Biol Chem. 2000;275:14882-9 pubmed..Our results show that transglycosidases anchored to the plasma membrane via glycosylphosphatidylinositols can play an active role in fungal cell wall synthesis. ..
- Muniz M, Nuoffer C, Hauri H, Riezman H. The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles. J Cell Biol. 2000;148:925-30 pubmed..Gap1p, which was not affected by emp24 mutation, was not cross-linked. These results suggest that the Emp24 complex acts as a cargo receptor in vesicle biogenesis from the ER. ..
- Sacristán Reviriego A, Madrid M, Cansado J, Martín H, Molina M. A conserved non-canonical docking mechanism regulates the binding of dual specificity phosphatases to cell integrity mitogen-activated protein kinases (MAPKs) in budding and fission yeasts. PLoS ONE. 2014;9:e85390 pubmed publisher..These results provide mechanistic insight into MKP-MAPK interplay and stress the relevance of this conserved non-canonical docking site in the regulation of the CWI pathway in fungi. ..
- Hsu J, Chang L, Jang L, Huang C, Lee F. The N-terminus of Vps74p is essential for the retention of glycosyltransferases in the Golgi but not for the modulation of apical polarized growth in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e74715 pubmed publisher..Thus, we propose that Vps74p may use different domains to interact with specific effectors thereby differentially modulating a variety of cellular functions. ..
- Morsomme P, Prescianotto Baschong C, Riezman H. The ER v-SNAREs are required for GPI-anchored protein sorting from other secretory proteins upon exit from the ER. J Cell Biol. 2003;162:403-12 pubmed..Therefore, we propose that v-SNAREs are part of the cargo protein sorting machinery upon exit from the ER and that a correct sorting process is necessary for proper maturation of GPI-anchored proteins. ..
- De Sampaïo G, Bourdineaud J, Lauquin G. A constitutive role for GPI anchors in Saccharomyces cerevisiae: cell wall targeting. Mol Microbiol. 1999;34:247-56 pubmed..Moreover, TIR1 proved to be a multicopy suppressor of Deltagas1 mutation. The present findings suggest a constitutive role for GPI anchors in yeast: the cell wall targeting of proteins. ..
- Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmedThe GAS1-related genes of fungi encode GPI-anchored proteins with beta-1,3-glucanosyltransferase activity. Loss of this activity results in defects in the assembly of the cell wall...
- Kang C, Jiang Y. Genome-wide survey of non-essential genes required for slowed DNA synthesis-induced filamentous growth in yeast. Yeast. 2005;22:79-90 pubmed..known BNI1, BUD2, PEA2, SPA2 and TPM1; (b) genes that are likely involved in cell wall biosynthesis, ECM25, GAS1 and PRS3; (c) genes encoding possible regulators of protein secretion, SEC66, RPL21A and RPL34B; (d) genes encoding ..
- Friedmann E, Salzberg Y, Weinberger A, Shaltiel S, Gerst J. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J Biol Chem. 2002;277:35274-81 pubmed..Yos9 binds directly to Gas1 and Mkc7 and accelerates Gas1 transport and processing in cells overexpressing YOS9...
- Cabib E, Blanco N, Grau C, Rodriguez Peña J, Arroyo J. Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall. Mol Microbiol. 2007;63:921-35 pubmed..Crh1p and Crh2p are the first proteins directly implicated in the formation of cross-links between cell wall components in fungi. ..
- Ram A, Kapteyn J, Montijn R, Caro L, Douwes J, Baginsky W, et al. Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. J Bacteriol. 1998;180:1418-24 pubmedDeletion of GAS1/GGP1/CWH52 results in a lower beta-glucan content of the cell wall and swollen, more spherical cells (L. Popolo, M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina, J. Bacteriol...
- Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmedThe fungal GAS1-related genes encode GPI-anchored beta-1,3-glucanosyltransferase, and their loss causes a defect in the assembly of the cell wall...
- Turchini A, Ferrario L, Popolo L. Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae. J Bacteriol. 2000;182:1167-71 pubmed..b>GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.
- Rolli E, Ragni E, de Medina Redondo M, Arroyo J, de Aldana C, Popolo L. Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:1585-98 pubmed publisher..b>GAS1 and GAS5 are expressed in vegetative cells and repressed during sporulation while GAS2 and GAS4 exhibit a ..
- He X, Qian W, Wang Z, Li Y, Zhang J. Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks. Nat Genet. 2010;42:272-6 pubmed publisher..We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs. ..
- Gualtieri T, Ragni E, Mizzi L, Fascio U, Popolo L. The cell wall sensor Wsc1p is involved in reorganization of actin cytoskeleton in response to hypo-osmotic shock in Saccharomyces cerevisiae. Yeast. 2004;21:1107-20 pubmed..In the gas1 null mutant cells, which lack a beta(1,3)-glucanosyltransferase activity required for cell wall assembly, a ..
- Liu Y, Lee S, Lee F. Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane. J Cell Sci. 2006;119:3845-55 pubmed..Thus, we infer that the signaling cascade Sys1p-Arl3p-Arl1p-Imh1p specifically participates in the transport of a GPI-anchored protein from the late Golgi to the plasma membrane. ..
- Ast T, Cohen G, Schuldiner M. A network of cytosolic factors targets SRP-independent proteins to the endoplasmic reticulum. Cell. 2013;152:1134-45 pubmed publisher..These findings highlight the underappreciated complexity of SRP-independent translocation, which enables this pathway to efficiently cope with its extensive substrate flux...
- Popolo L, Gilardelli D, Bonfante P, Vai M. Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae. J Bacteriol. 1997;179:463-9 pubmedThe GGP1/GAS1 gene codes for a glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of Saccharomyces cerevisiae. The ggp1delta mutant shows morphogenetic defects which suggest changes in the cell wall matrix...
- Luo G, Costanzo M, Boone C, Dickson R. Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast. J Biol Chem. 2011;286:8759-70 pubmed publisher..Mammals may use similar regulatory mechanisms because components of the decay apparatus and signaling pathways are conserved. ..
- Loukin S, Zhou X, Kung C, Saimi Y. A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast. FASEB J. 2008;22:2405-15 pubmed publisher..It appears that stress on the cell wall induces Ca(2+) accumulation, adaptively anticipating the need in defense or repair against future stress, including osmotic stress. ..
- Eustice M, Pillus L. Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiae. Genetics. 2014;196:1029-39 pubmed publisher..Although initially characterized in remodeling cell wall glucans, the ?-1,3-glucanosyltransferase Gas1 was recently discovered to regulate transcriptional silencing in a manner separable from its activity at the cell ..
- Valdivieso M, Ferrario L, Vai M, Duran A, Popolo L. Chitin synthesis in a gas1 mutant of Saccharomyces cerevisiae. J Bacteriol. 2000;182:4752-7 pubmed..synthesis, we tested chitin synthase I (CSI), CSII, and CSIII in vitro activities in the cell-wall-defective mutant gas1 delta...
- Ha C, Kim K, Chang Y, Kim B, Huh W. The ?-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae. Nucleic Acids Res. 2014;42:8486-99 pubmed publisher..Recently, a ?-1,3-glucanosyltransferase Gas1 has been shown to play a significant role in the regulation of transcriptional silencing in S. cerevisiae...
- Tabuchi M, Audhya A, Parsons A, Boone C, Emr S. The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol Cell Biol. 2006;26:5861-75 pubmed..Together, our data suggest that Slm1 and Slm2 define a molecular link between phosphoinositide and sphingolipid signaling and thereby regulate actin cytoskeleton organization. ..
- Huang C, Chang A. pH-dependent cargo sorting from the Golgi. J Biol Chem. 2011;286:10058-65 pubmed publisher..Because the missorted cargos have distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the Golgi. ..
- Jorgensen P, Nelson B, Robinson M, Chen Y, Andrews B, Tyers M, et al. High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants. Genetics. 2002;162:1091-9 pubmed..In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach. ..
- Plotnikova T, Selyakh I, Kalebina T, Kulaev I. Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall. Dokl Biochem Biophys. 2006;409:244-7 pubmed
- Aimanianda V, Simenel C, Garnaud C, Clavaud C, Tada R, Barbin L, et al. The Dual Activity Responsible for the Elongation and Branching of Î²-(1,3)-Glucan in the Fungal Cell Wall. MBio. 2017;8: pubmed publisher..at identifying deletion mutants with reduced branching among them revealed only two, the bgl2Î” and gas1Î” mutants, showing 15% and 70% reductions in the branching, respectively, compared to the wild-type strain...
- Surma M, Klose C, Klemm R, Ejsing C, Simons K. Generic sorting of raft lipids into secretory vesicles in yeast. Traffic. 2011;12:1139-47 pubmed publisher..This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation...
- Castillon G, Aguilera Romero A, Manzano López J, Epstein S, Kajiwara K, Funato K, et al. The yeast p24 complex regulates GPI-anchored protein transport and quality control by monitoring anchor remodeling. Mol Biol Cell. 2011;22:2924-36 pubmed publisher..Therefore the p24 complex, by sensing the status of the GPI anchor, regulates GPI-anchored protein intracellular transport and coordinates this with correct anchor remodeling. ..
- Tomishige N, Noda Y, Adachi H, Yoda K. SKG6, a suppressor gene of synthetic lethality of kex2Delta gas1Delta mutations, encodes a novel membrane protein showing polarized intracellular localization. J Gen Appl Microbiol. 2005;51:323-6 pubmed
- Carotti C, Ferrario L, Roncero C, Valdivieso M, Duran A, Popolo L. Maintenance of cell integrity in the gas1 mutant of Saccharomyces cerevisiae requires the Chs3p-targeting and activation pathway and involves an unusual Chs3p localization. Yeast. 2002;19:1113-24 pubmed..activation pathway of Chs3p or through an alternative one, here we studied the effects of the inactivation of the GAS1 and CHS4-5-6-7 genes...
- Koch M, Pillus L. The glucanosyltransferase Gas1 functions in transcriptional silencing. Proc Natl Acad Sci U S A. 2009;106:11224-9 pubmed publisher..Here, we report an unsuspected role for the enzyme Gas1 in locus-specific transcriptional silencing...
- Bickle M, Delley P, Schmidt A, Hall M. Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J. 1998;17:2235-45 pubmed..We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts)...