Gene Symbol: GAS1
Description: 1,3-beta-glucanosyltransferase GAS1
Alias: CWH52, GGP1, 1,3-beta-glucanosyltransferase GAS1
Species: Saccharomyces cerevisiae S288c
Products:     GAS1

Top Publications

  1. Rolli E, Ragni E, Rodriguez Peña J, Arroyo J, Popolo L. GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae. Yeast. 2010;27:597-610 pubmed publisher
    ..b>GAS1 and GAS5 are expressed in vegetative growth and repressed during sporulation, whereas GAS2 and GAS4 exhibit the ..
  2. Rolli E, Ragni E, Calderon J, Porello S, Fascio U, Popolo L. Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast. Mol Biol Cell. 2009;20:4856-70 pubmed publisher
    ..We present evidence that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar. These results reveal a new mechanism of protein immobilization at specific sites of the cell envelope. ..
  3. Fujita M, Yoko o T, Jigami Y. Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins. Mol Biol Cell. 2006;17:834-50 pubmed
    ..We have constructed a mutant form of the beta-1,3-glucanosyltransferase Gas1p (Gas1*p) as a model misfolded GPI-anchored protein...
  4. Watanabe R, Castillon G, Meury A, Riezman H. The presence of an ER exit signal determines the protein sorting upon ER exit in yeast. Biochem J. 2008;414:237-45 pubmed publisher
    ..We attempted to identify sorting determinants for this protein sorting event in the ER. We found that mutant Gas1 proteins that lack a GPI anchor and/or S/T region (serine- and threonine-rich region), two common characteristic ..
  5. Ragni E, Fontaine T, Gissi C, Latge J, Popolo L. The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast. 2007;24:297-308 pubmed
    The GAS multigene family of Saccharomyces cerevisiae is constituted by five genes (GAS1-GAS5), but GAS1 was the only one to have been characterized to date...
  6. Yin Q, De Groot P, Dekker H, De Jong L, Klis F, de Koster C. Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages. J Biol Chem. 2005;280:20894-901 pubmed
    ..Therefore, it will probably be equally effective in many other fungi. ..
  7. Carotti C, Ragni E, Palomares O, Fontaine T, Tedeschi G, Rodriguez R, et al. Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding. Eur J Biochem. 2004;271:3635-45 pubmed
    ..Purified soluble Gas1 protein showed beta-(1,3)-glucanosyltransferase activity that was abolished by replacement of the putative ..
  8. Bagnat M, Keranen S, Shevchenko A, Simons K. Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast. Proc Natl Acad Sci U S A. 2000;97:3254-9 pubmed
    ..Using the sec18-1 mutant we found that DIGs are present already in the ER. Taken together, our results suggest that lipid rafts are involved in the biosynthetic delivery of proteins to the yeast plasma membrane. ..
  9. Nuoffer C, Jeno P, Conzelmann A, Riezman H. Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol. 1991;11:27-37 pubmed
    ..We have cloned the gene (GAS1) encoding the 125-kDa protein (Gas1p) and found that the function of Gas1p is not essential for cell viability...

More Information


  1. Mouyna I, Fontaine T, Vai M, Monod M, Fonzi W, Diaquin M, et al. Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall. J Biol Chem. 2000;275:14882-9 pubmed
    ..Our results show that transglycosidases anchored to the plasma membrane via glycosylphosphatidylinositols can play an active role in fungal cell wall synthesis. ..
  2. Muniz M, Nuoffer C, Hauri H, Riezman H. The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles. J Cell Biol. 2000;148:925-30 pubmed
    ..Gap1p, which was not affected by emp24 mutation, was not cross-linked. These results suggest that the Emp24 complex acts as a cargo receptor in vesicle biogenesis from the ER. ..
  3. Sacristán Reviriego A, Madrid M, Cansado J, Martín H, Molina M. A conserved non-canonical docking mechanism regulates the binding of dual specificity phosphatases to cell integrity mitogen-activated protein kinases (MAPKs) in budding and fission yeasts. PLoS ONE. 2014;9:e85390 pubmed publisher
    ..These results provide mechanistic insight into MKP-MAPK interplay and stress the relevance of this conserved non-canonical docking site in the regulation of the CWI pathway in fungi. ..
  4. Hsu J, Chang L, Jang L, Huang C, Lee F. The N-terminus of Vps74p is essential for the retention of glycosyltransferases in the Golgi but not for the modulation of apical polarized growth in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e74715 pubmed publisher
    ..Thus, we propose that Vps74p may use different domains to interact with specific effectors thereby differentially modulating a variety of cellular functions. ..
  5. Morsomme P, Prescianotto Baschong C, Riezman H. The ER v-SNAREs are required for GPI-anchored protein sorting from other secretory proteins upon exit from the ER. J Cell Biol. 2003;162:403-12 pubmed
    ..Therefore, we propose that v-SNAREs are part of the cargo protein sorting machinery upon exit from the ER and that a correct sorting process is necessary for proper maturation of GPI-anchored proteins. ..
  6. De Sampaïo G, Bourdineaud J, Lauquin G. A constitutive role for GPI anchors in Saccharomyces cerevisiae: cell wall targeting. Mol Microbiol. 1999;34:247-56 pubmed
    ..Moreover, TIR1 proved to be a multicopy suppressor of Deltagas1 mutation. The present findings suggest a constitutive role for GPI anchors in yeast: the cell wall targeting of proteins. ..
  7. Jamaluddin M, Bailey U, Schulz B. Oligosaccharyltransferase subunits bind polypeptide substrate to locally enhance N-glycosylation. Mol Cell Proteomics. 2014;13:3286-93 pubmed publisher
  8. Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmed
    The GAS1-related genes of fungi encode GPI-anchored proteins with beta-1,3-glucanosyltransferase activity. Loss of this activity results in defects in the assembly of the cell wall...
  9. Kang C, Jiang Y. Genome-wide survey of non-essential genes required for slowed DNA synthesis-induced filamentous growth in yeast. Yeast. 2005;22:79-90 pubmed
    ..known BNI1, BUD2, PEA2, SPA2 and TPM1; (b) genes that are likely involved in cell wall biosynthesis, ECM25, GAS1 and PRS3; (c) genes encoding possible regulators of protein secretion, SEC66, RPL21A and RPL34B; (d) genes encoding ..
  10. Friedmann E, Salzberg Y, Weinberger A, Shaltiel S, Gerst J. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J Biol Chem. 2002;277:35274-81 pubmed
    ..Yos9 binds directly to Gas1 and Mkc7 and accelerates Gas1 transport and processing in cells overexpressing YOS9...
  11. Cabib E, Blanco N, Grau C, Rodriguez Peña J, Arroyo J. Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall. Mol Microbiol. 2007;63:921-35 pubmed
    ..Crh1p and Crh2p are the first proteins directly implicated in the formation of cross-links between cell wall components in fungi. ..
  12. Ram A, Kapteyn J, Montijn R, Caro L, Douwes J, Baginsky W, et al. Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. J Bacteriol. 1998;180:1418-24 pubmed
    Deletion of GAS1/GGP1/CWH52 results in a lower beta-glucan content of the cell wall and swollen, more spherical cells (L. Popolo, M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina, J. Bacteriol...
  13. Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmed
    The fungal GAS1-related genes encode GPI-anchored beta-1,3-glucanosyltransferase, and their loss causes a defect in the assembly of the cell wall...
  14. Turchini A, Ferrario L, Popolo L. Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae. J Bacteriol. 2000;182:1167-71 pubmed
    ..b>GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.
  15. Rolli E, Ragni E, de Medina Redondo M, Arroyo J, de Aldana C, Popolo L. Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:1585-98 pubmed publisher
    ..b>GAS1 and GAS5 are expressed in vegetative cells and repressed during sporulation while GAS2 and GAS4 exhibit a ..
  16. He X, Qian W, Wang Z, Li Y, Zhang J. Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks. Nat Genet. 2010;42:272-6 pubmed publisher
    ..We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs. ..
  17. Gualtieri T, Ragni E, Mizzi L, Fascio U, Popolo L. The cell wall sensor Wsc1p is involved in reorganization of actin cytoskeleton in response to hypo-osmotic shock in Saccharomyces cerevisiae. Yeast. 2004;21:1107-20 pubmed
    ..In the gas1 null mutant cells, which lack a beta(1,3)-glucanosyltransferase activity required for cell wall assembly, a ..
  18. Liu Y, Lee S, Lee F. Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane. J Cell Sci. 2006;119:3845-55 pubmed
    ..Thus, we infer that the signaling cascade Sys1p-Arl3p-Arl1p-Imh1p specifically participates in the transport of a GPI-anchored protein from the late Golgi to the plasma membrane. ..
  19. Kováčová K, Farkaš V. Two Variants of a High-Throughput Fluorescent Microplate Assay of Polysaccharide Endotransglycosylases. Appl Biochem Biotechnol. 2016;178:1652-65 pubmed publisher
  20. Ast T, Cohen G, Schuldiner M. A network of cytosolic factors targets SRP-independent proteins to the endoplasmic reticulum. Cell. 2013;152:1134-45 pubmed publisher
    ..These findings highlight the underappreciated complexity of SRP-independent translocation, which enables this pathway to efficiently cope with its extensive substrate flux...
  21. Popolo L, Gilardelli D, Bonfante P, Vai M. Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae. J Bacteriol. 1997;179:463-9 pubmed
    The GGP1/GAS1 gene codes for a glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of Saccharomyces cerevisiae. The ggp1delta mutant shows morphogenetic defects which suggest changes in the cell wall matrix...
  22. Luo G, Costanzo M, Boone C, Dickson R. Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast. J Biol Chem. 2011;286:8759-70 pubmed publisher
    ..Mammals may use similar regulatory mechanisms because components of the decay apparatus and signaling pathways are conserved. ..
  23. Loukin S, Zhou X, Kung C, Saimi Y. A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast. FASEB J. 2008;22:2405-15 pubmed publisher
    ..It appears that stress on the cell wall induces Ca(2+) accumulation, adaptively anticipating the need in defense or repair against future stress, including osmotic stress. ..
  24. Eustice M, Pillus L. Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiae. Genetics. 2014;196:1029-39 pubmed publisher
    ..Although initially characterized in remodeling cell wall glucans, the ?-1,3-glucanosyltransferase Gas1 was recently discovered to regulate transcriptional silencing in a manner separable from its activity at the cell ..
  25. Mohd Yusuf S, Bailey U, Tan N, Jamaluddin M, Schulz B. Mixed disulfide formation in vitro between a glycoprotein substrate and yeast oligosaccharyltransferase subunits Ost3p and Ost6p. Biochem Biophys Res Commun. 2013;432:438-43 pubmed publisher
  26. Valdivieso M, Ferrario L, Vai M, Duran A, Popolo L. Chitin synthesis in a gas1 mutant of Saccharomyces cerevisiae. J Bacteriol. 2000;182:4752-7 pubmed
    ..synthesis, we tested chitin synthase I (CSI), CSII, and CSIII in vitro activities in the cell-wall-defective mutant gas1 delta...
  27. Ha C, Kim K, Chang Y, Kim B, Huh W. The ?-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae. Nucleic Acids Res. 2014;42:8486-99 pubmed publisher
    ..Recently, a ?-1,3-glucanosyltransferase Gas1 has been shown to play a significant role in the regulation of transcriptional silencing in S. cerevisiae...
  28. Tabuchi M, Audhya A, Parsons A, Boone C, Emr S. The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol Cell Biol. 2006;26:5861-75 pubmed
    ..Together, our data suggest that Slm1 and Slm2 define a molecular link between phosphoinositide and sphingolipid signaling and thereby regulate actin cytoskeleton organization. ..
  29. Huang C, Chang A. pH-dependent cargo sorting from the Golgi. J Biol Chem. 2011;286:10058-65 pubmed publisher
    ..Because the missorted cargos have distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the Golgi. ..
  30. Jorgensen P, Nelson B, Robinson M, Chen Y, Andrews B, Tyers M, et al. High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants. Genetics. 2002;162:1091-9 pubmed
    ..In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach. ..
  31. Plotnikova T, Selyakh I, Kalebina T, Kulaev I. Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall. Dokl Biochem Biophys. 2006;409:244-7 pubmed
  32. Aimanianda V, Simenel C, Garnaud C, Clavaud C, Tada R, Barbin L, et al. The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall. MBio. 2017;8: pubmed publisher identifying deletion mutants with reduced branching among them revealed only two, the bgl2Δ and gas1Δ mutants, showing 15% and 70% reductions in the branching, respectively, compared to the wild-type strain...
  33. Surma M, Klose C, Klemm R, Ejsing C, Simons K. Generic sorting of raft lipids into secretory vesicles in yeast. Traffic. 2011;12:1139-47 pubmed publisher
    ..This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation...
  34. Castillon G, Aguilera Romero A, Manzano López J, Epstein S, Kajiwara K, Funato K, et al. The yeast p24 complex regulates GPI-anchored protein transport and quality control by monitoring anchor remodeling. Mol Biol Cell. 2011;22:2924-36 pubmed publisher
    ..Therefore the p24 complex, by sensing the status of the GPI anchor, regulates GPI-anchored protein intracellular transport and coordinates this with correct anchor remodeling. ..
  35. Tomishige N, Noda Y, Adachi H, Yoda K. SKG6, a suppressor gene of synthetic lethality of kex2Delta gas1Delta mutations, encodes a novel membrane protein showing polarized intracellular localization. J Gen Appl Microbiol. 2005;51:323-6 pubmed
  36. Carotti C, Ferrario L, Roncero C, Valdivieso M, Duran A, Popolo L. Maintenance of cell integrity in the gas1 mutant of Saccharomyces cerevisiae requires the Chs3p-targeting and activation pathway and involves an unusual Chs3p localization. Yeast. 2002;19:1113-24 pubmed
    ..activation pathway of Chs3p or through an alternative one, here we studied the effects of the inactivation of the GAS1 and CHS4-5-6-7 genes...
  37. Koch M, Pillus L. The glucanosyltransferase Gas1 functions in transcriptional silencing. Proc Natl Acad Sci U S A. 2009;106:11224-9 pubmed publisher
    ..Here, we report an unsuspected role for the enzyme Gas1 in locus-specific transcriptional silencing...
  38. Bickle M, Delley P, Schmidt A, Hall M. Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J. 1998;17:2235-45 pubmed
    ..We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts)...